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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 563-569.doi: 10.3724/SP.J.1006.2013.00563

• RESEARCH NOTES • Previous Articles    

Expression of Cotton GhPRP5 Gene in Arabidopsis Enhances Susceptibility to ABA and Salt Stresses

ZHANG De-Jing,QIN Li-Xia,LI Long,RAO Yue,LI Xue-Bao,XU Wen-Liang*   

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology / College of Life Sciences, Central China Normal University, Wuhan 430079, China
  • Received:2012-07-10 Revised:2012-10-09 Online:2013-03-12 Published:2012-12-11
  • Contact: 许文亮, E-mail: wenliangxu@yahoo.com.cn, Tel: 027-67867814

Abstract:

Pro-rich proteins (PRPs) represent one family of Pro- and Hyp-rich structural cell wall proteins that are initially identified as wound-induced gene products in carrot storage roots. Accumulated evidences demonstrate that PRP genes are regulated by various abiotic and biotic stresses and may play a role in plant responses to changes in living conditions. In our previous study, a gene encoding a proline-rich protein designated as GhPRP5 was isolated from cotton cDNA libraries. To validate its function, in this study, we introduced the coding region of GhPRP5into the vector pBI121 under the control of the CaMV 35S promoter and then transformed the vector into Arabidopsis thaliana. Eight independent T4 homozygous lines with high expression of GhPRP5 were obtained. Germination rate of transgenic lines overexpressing GhPRP5 was not affected under normal conditions; however, salt stress and ABA significantly inhibited the germination of the transgenic lines. When growing on media with NaCl, the GhPRP5-overexpressed plants displayed much less cotyledon greening rate compared with the wild type. In contrast to the normal growth conditions, ABA inhibited the elongation of primary root more severely in the transgenic lines. Quantitative RT-PCR technique was used to analyze the transcription of several stress gene markers (RD29A, RD29B, KIN1,and ABI1) in the transgenic lines and the wild type plants under salt stress and ABA treatments. Expressions of RD29A, RD29B, and KIN1 were induced by ABA and NaCl in the transgenic and the wild type plants, though the induction levels in the transgenic lines were different from those in the wild type. This finding suggests that GhPRP5 is implicated in the regulation of stress gene expression in Arabidopsis. The plant stress signal transduction pathway in which GhPRP5 may be involved needs to be further studied.

Key words: GhPRP5, Transgenic Arabidopsis, Salt stress, ABA

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