Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1775-1782.doi: 10.3724/SP.J.1006.2013.01775

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of NPR1-like Genes and their Response to Fusarium graminearum Infection in Wheat

YANG Zai-Dong,MA Xin,WU Shi-Wen,WANG Hong-Wei,SU Xin,JI Xian-Ling,LI An-Fei,KONG Ling-Rang*   

  1. State Key Laboratory of Crop Biology / College of Agronomy, Shandong Agricultural University, Tai’an 271018, China
  • Received:2013-03-07 Revised:2013-05-24 Online:2013-10-12 Published:2013-08-01
  • Contact: 孔令让, E-mail:lkong@sdau.edu.cn

Abstract:

NPR1 gene plays a key role for systemic acquired resistance (SAR) and provides broad-spectrum resistance in Arabidopsis. Fusarium head blight (FHB), which is caused by Fusarium graminearum Schwabe, is a devastating disease of wheat worldwide. Based on the analysis of the gene expression profiling, we clonedthree NPR1-likegenes from wheat FHB near-isogenic lines induced by F. graminearum using RT-PCR protocol, and they were designated as TaNPR1, TaNPR2,and TaNPR3. The open reading frames of the three genes encoded 580, 607, and 601 amino acid residues, respectively. The three wheat NPR1-like proteins had conversed BTB/POZ, ANK, and NPR1_like_C domain as well as conversed amino acid residues with important functions. However, only TaNPR1 had two conversed cysteine residues that are essential for the NPR1 oligomer formation. Phylogenetic analysis showed that TaNPR1 was involved in the NPR1 protein group, while TaNPR2 and TaNPR3 were close to NPR1 homologues. Quantitative RT-PCR assay revealed that the three NPR1-like genes could be induced by defense related signal molecules, such as salicylic acid and methyl jasmonate. TaNPR1 and TaNPR3 were induced earlier and up-regulated more significantly in response to F. graminearum infection in the resistant line Apogee73S2 than in the susceptible line Apogee. However, the transcription of TaNPR2 was not obviously changed in either the resistant or susceptible near-isogenic lines after inoculation with F. graminearum. These results suggest that TaNPR1 and TaNPR3 may be involved in the defense response to F. graminearum.

Key words: Triticum aestivum, NPR1-like genes, Fusarium head blight (FHB), Expression analysis

[1]Durrant W E, Dong X N. Systemic acquired resistance. Annu Rev Phytopathol, 2004, 42: 185–209



[2]Dong X N. NPR1, all things considered. Curr Opin Plant Biol, 2004, 7: 547–552



[3]Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, Uknes S, Ward E, Kessmann H, Ryals J. Requirement of salicylic acid for the induction of systemic acquired resistance. Science, 1993, 261: 754–756



[4]Mukhtar M S, Nishimura M T, Dangl J. NPR1 in Plant Defense: It’s not over’til it’s turned over. Cell, 2009, 137: 804–806



[5]Cao H, Glazebrook J, Clarke J D, Volko S, Dong X N. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell, 1997, 88: 57–63



[6]Aravind L, Koonin E V.Fold prediction and evolutionary analysis of the POZ domain: structural and evolutionary relationship with the potassium channel telramefization domain. J Mol Biol, 1999, 285: 1353–1361



[7]Kinkema M, Fan W H, Dong X N. Nuclear localization of NPR1 is required for activation of PR gene expression. Plant Cell, 2000, 12: 2339–2350



[8]Mou Z L, Fan W H, Dong X N. Inducers of plant systemic acquired resistance regulate NPR1 function through Redox changes. Cell, 2003, 113: 935–944



[9]Pieterse C M J, Van Wees S C M, Van Pelt J A, Knoester M, Laan R, Gerrits H, Weisbeek P J, d Van Loon L C. A novel signaling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell, 1998, 10: 1571–1580



[10]Chern M, Fitzgerald H A, Canlas P E, Navarre D A, Ronald P C. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant-Microbe Interact, 2005, 18: 511–520



[11]Zhang L, Zhang Y, Shi J, Zhang L, Guo X Q. Molecular cloning and characterization of a novel NPR1 gene from Nicotiana glutinosa. Acta Phytopathol Sin, 2009, 39: 482–490



[12]Tang Y-M(唐益苗), Zhang Z-Y(张增艳), Xin Z-Y(辛志勇). Isolation and characterization of NPR1 homolog gene TiNH1 in Thinopyrum intermedium. Sci Agric Sin (中国农业科学), 2007, 40(6): 1101–1107 (in Chinese with English abstract)



[13]Zhang Y, Wang X, Cheng C, Gao Q, Liu J, Guo X. Molecular cloning and characterization of GhNPR1, a gene implicated in pathogen responses from cotton (Gossypium hirsutum L.). Biosci Rep, 2008, 28: 7–14



[14]Malnoy M, Jin Q, Borejsza-Wysocka E E, He S Y, Aldwinckle H S. Overexpression of the apple MpNPR1 gene confers increased disease resistance in Malus × domestica. Mol Plant-Microbe Interact, 2007, 20: 1568–1580



[15]Chern M S, Fitzgerald H A, Yadav R C, Canlas P E, Dong X N, Ronald P C. Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. Plant J, 2001, 27: 101–113



[16]Cao H, Li X, Dong X N. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance. Proc Natl Acad Sci USA, 1998, 95: 6531–6536



[17]Desjardins A E, Hohn T M. Mycotoxins in plant pathogenesis. Mol Plant-Microbe Interact, 1997, 10: 147–152



[18]Makandar R, Essig J S, Schapaugh M A, Trick H N, Shah J. Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1. Mol Plant-Microbe Interact, 2006, 19: 123–129



[19]Fu Z Q, Yan S P, Saleh A, Wang W, Ruble J, Oka N, Mohan R, Spoel S H, Tada Y, Zheng N, Dong X N. NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants. Nature, 2012, 486: 228–233



[20]Jia H Y, Cho S, Muehlbauer G J. Transcriptome analysis of a wheat near-isogenic line pair carrying Fusarium head blight resistant and susceptible alleles. Mol Plant-Microbe Interact, 2009, 22: 1366–1378



[21]Glazebrook J, Rogers E E, Ausubel F M. Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening. Genetics, 1996, 143: 973–982



[22]Wastemack C, Parthier B. Jasmonate signaled plant gene expression. Trends Plant Sci, 1997, 2: 302–307



[23]Zhang Y, Fan W H, Kinkema M, LI X, Dong X N. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc Natl Acad Sci USA, 1999, 96: 6523–6528



[24]Yuan Y X, Zhong S H, Li Q, Zhu Z R, Lou Y G, Wang L Y, Wang J J, Wang M Y, Li Q L, Yang D L, He Z H. Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility. Plant Biotechnol J, 2007, 5: 313–324



[25]Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol, 2005,43: 205–227

[1] Zuo Tong-Hong, Zhang He-Cui, Zeng Jing, Zhu Li-Quan. Molecular cloning and expression analysis of BoPUB3L associated with self-incompatibility in Brasscia oleracea [J]. Acta Agronomica Sinica, 2026, 52(6): 1698-1710.
[2] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[3] Tian Li-Tao, Ding Ning, Wang Shu-Lin, Qi En-Fang, Zhang Rong, Wang Rui-Rui, Ma Li-Wen, Li Jian-Wu, Yang Jiang-Wei. Genome-wide identification of the Argonaute gene family and its induction by late blight in potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2026, 52(4): 1116-1126.
[4] Yang Zong-Tao, Yang Ting, Wang Yu-Tong, Ai Jing, Li Yan-Ye, Liu Jia-Yong, Deng Jun, Zhao Yong, Zhang Yue-Bin. Identification and expression analysis of the CLC gene family in sugarcane [J]. Acta Agronomica Sinica, 2026, 52(3): 722-734.
[5] Meng Cheng, Wang Zhe. Genome-wide identification and expression analysis of the ZmPFK gene family under biotic and abiotic stresses in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 764-779.
[6] Zhang Li-Lan, Yang Jun, Wang Rang-Jian. Identification of candidate genes related to glycoside aroma precursor content in tea plant using WGCNA [J]. Acta Agronomica Sinica, 2026, 52(2): 494-513.
[7] WANG Bin, MENG Jiang-Yu, QIU Hao-Liang, HE Ya-Jun, QIAN Wei. Identification and expression pattern analysis of the BnaDUF579 gene family in Brassica napus [J]. Acta Agronomica Sinica, 2025, 51(8): 2100-2110.
[8] GUO Teng-Da, CUI Meng-Jie, CHEN Lin-Jie, HAN Suo-Yi, GUO Jing-Kun, WU Chen-Di, FU Liu-Yang, HUANG Bing-Yan, DONG Wen-Zhao, ZHANG Xin-You. Cloning and expression analysis of the phosphatidylinositol transfer protein AhSFH gene in peanuts responsive to Aspergillus flavus infection [J]. Acta Agronomica Sinica, 2025, 51(6): 1489-1500.
[9] PAN Ju-Zhong, WEI Ping, ZHU De-Ping, SHAO Sheng-Xue, CHEN Shan-Shan, WEI Ya-Qian, GAO Wei-Wei. Cloning and functional analysis of OsERF104 transcription factor in rice [J]. Acta Agronomica Sinica, 2025, 51(4): 900-913.
[10] QI Jia-Min, XU Chun-Miao, XIAO Bin. Genome-wide identification and expression analysis of TIFY gene family in potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2024, 50(9): 2297-2309.
[11] CAO Song, YAO Min, REN Rui, JIA Yuan, XIANG Xing-Ru, LI Wen, HE Xin, LIU Zhong-Song, GUAN Chun-Yun, QIAN Lun-Wen, XIONG Xing-Hua. A combination of genome-wide association and transcriptome analysis reveal candidate genes affecting seed oil accumulation in Brassica napus [J]. Acta Agronomica Sinica, 2024, 50(5): 1136-1146.
[12] LI Hai-Fen, LU Qing, LIU Hao, WEN Shi-Jie, WANG Run-Feng, HUANG Lu, CHEN Xiao-Ping, HONG Yan-Bin, LIANG Xuan-Qiang. Genome-wide identification and expression analysis of AhGA3ox gene family in peanut (Arachis hypogaea L.) [J]. Acta Agronomica Sinica, 2024, 50(4): 932-943.
[13] DAI Hong-Wei, LIU Jie-Qiang, ZHANG Li, TONG Hua-Rong, YUAN Lian-Yu. Cloning and relative expression pattern analysis of CsMCC1 and CsMCC2 in tea plant (Camellia sinensis) [J]. Acta Agronomica Sinica, 2024, 50(3): 656-668.
[14] YIN Xiang-Zhen, ZHAO Jian-Xin, HAO Cui-Cui, PAN Li-Juan, CHEN Na, XU Jing, JIANG Xiao, ZHAO Xu-Hong, WANG En-Qi, CAO Huan, YU Shan-Lin, CHI Xiao-Yuan. Cloning and expression analysis of transcription factor AhWRI1s in peanut [J]. Acta Agronomica Sinica, 2024, 50(12): 3155-3164.
[15] DAI Shu-Tao, ZHU Can-Can, MA Xiao-Qian, QIN Na, SONG Ying-Hui, WEI Xin, WANG Chun-Yi, LI Jun-Xia. Genome-wide identification of the HAK/KUP/KT potassium transporter family in foxtail millet and its response to K+ deficiency and high salt stress [J]. Acta Agronomica Sinica, 2023, 49(8): 2105-2121.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!