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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1783-1790.doi: 10.3724/SP.J.1006.2013.01783

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Evaluation for Resistance to Sclerotinia Stem Rot in 44 Soybean Mini Core Collections

HAN Fen-Xia,HAN Guang-Zhen,SUN Jun-Ming,ZHANG Jin-Wei,YU Shao-Xuan,YAN Shu-Rong,YANG Hua   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology (Beijing), Ministry of Agriculture, Beijing 100081, China
  • Received:2013-03-25 Revised:2013-06-09 Online:2013-10-12 Published:2013-07-31

Abstract:

Sclerotinia stem rot, also called white mould, is a fungal pathogen disease worldwide in soybean caused by Sclerotinia sclerotiorum (Lib.) de Bary. A total of 44 soybean cultivars were inoculated with four isolates of S. Sclerotiorum from different geographical regions and host plants in the two consecutive years experiment in the field to screen excellent germplasm for its resistance breeding. The results were as follows: (1) Different soybean cultivars had different resistance level to S. sclerotiorum. Among them, six cultivar (13.64%) were moderate resistant; twenty-seven (61.36%) were moderate susceptible; nine (20.45%) were susceptible; two (4.55%) were highly susceptible, showing that soybean cultivars with resistance to S. sclerotiorum are rare. Six soybean cultivars with moderate resistance, i.e. Hefeng 24, Big Swan egg, Nidinghuamei soybean, Niumaohuang, Big soybean and May soybean, could be used for soybean breeding for S. sclerotiorum resistance. (2) The pathogenicity of S. sclerotiorum isolates from different geographical regions and host plants is different. Isolate Heixi 5 from the soybean field of Heilongjiang had the strongest pathogenicity with 49.32 in DSI and 5.93 mm in lesion length. Isolate Heirao 24 from the soybean field of Heilongjiang and isolate Qin 24 from the oilseed rape field of Qinghai province had the moderate pathogenicity. Isolate Hef 50 from the oilseed rape field of Anhui province was the weakest in infection, with 39.02 in DSI and 3.65 mm in the lesion length. In conclusion, isolate Heixi 5 is the best one for effectively screening for the resistance to S. sclerotiorum in soybean breeding.

Key words: Soybean [Glycine max (L.) Merr.], Sclerotinia Sclerotiorum, Identification for resistance

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