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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1775-1782.doi: 10.3724/SP.J.1006.2013.01775

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of NPR1-like Genes and their Response to Fusarium graminearum Infection in Wheat

YANG Zai-Dong,MA Xin,WU Shi-Wen,WANG Hong-Wei,SU Xin,JI Xian-Ling,LI An-Fei,KONG Ling-Rang*   

  1. State Key Laboratory of Crop Biology / College of Agronomy, Shandong Agricultural University, Tai’an 271018, China
  • Received:2013-03-07 Revised:2013-05-24 Online:2013-10-12 Published:2013-08-01
  • Contact: 孔令让, E-mail:lkong@sdau.edu.cn

Abstract:

NPR1 gene plays a key role for systemic acquired resistance (SAR) and provides broad-spectrum resistance in Arabidopsis. Fusarium head blight (FHB), which is caused by Fusarium graminearum Schwabe, is a devastating disease of wheat worldwide. Based on the analysis of the gene expression profiling, we clonedthree NPR1-likegenes from wheat FHB near-isogenic lines induced by F. graminearum using RT-PCR protocol, and they were designated as TaNPR1, TaNPR2,and TaNPR3. The open reading frames of the three genes encoded 580, 607, and 601 amino acid residues, respectively. The three wheat NPR1-like proteins had conversed BTB/POZ, ANK, and NPR1_like_C domain as well as conversed amino acid residues with important functions. However, only TaNPR1 had two conversed cysteine residues that are essential for the NPR1 oligomer formation. Phylogenetic analysis showed that TaNPR1 was involved in the NPR1 protein group, while TaNPR2 and TaNPR3 were close to NPR1 homologues. Quantitative RT-PCR assay revealed that the three NPR1-like genes could be induced by defense related signal molecules, such as salicylic acid and methyl jasmonate. TaNPR1 and TaNPR3 were induced earlier and up-regulated more significantly in response to F. graminearum infection in the resistant line Apogee73S2 than in the susceptible line Apogee. However, the transcription of TaNPR2 was not obviously changed in either the resistant or susceptible near-isogenic lines after inoculation with F. graminearum. These results suggest that TaNPR1 and TaNPR3 may be involved in the defense response to F. graminearum.

Key words: Triticum aestivum, NPR1-like genes, Fusarium head blight (FHB), Expression analysis

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