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Cloning and expression analysis of transcription factor AhWRI1s in peanut

YIN Xiang-Zhen1,ZHAO Jian-Xin2,HAO Cui-Cui2,PAN Li-Juan1,CHEN Na1,XU Jing1,JIANG Xiao1,ZHAO Xu-Hong1,WANG En-Qi2,CAO Huan2,YU Shan-Lin1,CHI Xiao-Yuan1,*   

  1. 1 Shandong Peanut Research Institute, Qingdao 266100, Shandong, China;2 College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, China
  • Received:2024-03-29 Revised:2024-08-15 Accepted:2024-08-15 Published:2024-09-03
  • Supported by:
    This study was supported by the China Agriculture Research System of MOF and MARA (CARS-13), the Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2023F20, CXGC2024F20), the Key-Area Research and Development Program of Guangdong Province (2020B020219003), the Major Scientific and Technological Project in Xinjiang (2022A02008-3), the Taishan Scholar Project Funding, the Research and Development Program of Shandong Province (the Improved Variety Engineering Project) (2022LZGC007), the Natural Science Foundation of Shandong Province (ZR2021QC172, ZR2023QC146), and the Key Research and Development Plan of Shandong Province (Action Plan to Boost Scientific and Technological Innovation in Rural Revitalization) (2022TZXD0031).

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Abstract:

Peanut is one of the widely cultivated oil and economic crops worldwide and has become a major source of oil and protein for humans due to its high oil and protein content. With the increasing global demand for vegetable oil, improving the fatty acid composition and increasing the lipid content of peanut seeds has become a top priority in peanut breeding. Transcriptional regulators can modulate the expression of a series of genes in metabolic pathways related to lipid synthesis, significantly affecting lipid synthesis and metabolism. In this study, two transcription factors, AhWRI1-1 and AhWRI1-2, were cloned from the leaves of Huayu 33. The ORF of AhWRI1-1 was 1101 bp, encoding 366 amino acids, and the ORF of AhWRI1-2 was 1128 bp, encoding 375 amino acids. Bioinformatics analysis revealed that both AhWRI1-1 and AhWRI1-2 contained two AP2/EREBP conserved domains. The expression patterns of AhWRI1-1 and AhWRI1-2 in different tissues were detected by qRT-PCR. The results showed that AhWRI1-1 had the highest expression in seeds, suggesting its involvement in the regulation of fatty acid synthesis and oil accumulation, while AhWRI1-2 had the highest expression in hypocotyls, indicating its role in hypocotyl development. Additionally, the differences in the responses of AhWRI1-1 and AhWRI1-2 to abiotic stresses suggested that these transcription factors may play different roles under such conditions. Transcriptional activation experiments in yeast showed that both AhWRI1-1 and AhWRI1-2 possess transcriptional activation activities. This study lays the foundation for future in-depth functional studies of AhWRI1-1 and AhWRI1-2.

Key words: peanut, AP2/EREBP transcription factor, abiotic stress, gene expression analysis, transcriptional activation

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