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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 2039-2045.doi: 10.3724/SP.J.1006.2013.02039

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Short-medium Term Preservation of Rice Suspension Cells

GAO Yi-Ping1,2,LÜ Meng-Yu1,ZHAO He1,YANG Xue-Ju2,*,WANG Hai-Bo1,*   

  1. 1 Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Sciences, Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China; 2 College of Agriculture, Agricultural University of Hebei, Baoding 071001, China
  • Received:2013-05-13 Revised:2013-06-09 Online:2013-11-12 Published:2013-08-14
  • Contact: 王海波, E-mail: nkywanghb@yahoo.com.cn; 杨学举, E-mail: shmyxj@hebau.edu.cn E-mail:hebgyp@163.com

Abstract:

The preservation of cell suspension in short-medium term is widely needed in cell biology. In this paper, the preservation efects of cell suspension of rice cultivar Zhonghua 15 on AA, N6, and MS solid culture media were compared. The results showed that AA medium was suitable for suspension cells preservation. The cell suspension still kept resuspensible after growing continuously on AA solid medium for 4 months, and then on AA medium supplemented with 0.5 mg L-1 2,4-D for 6 months (subcultured at about 45 d interval). Three kinds of methods of suspension cells preservation, namely preservation on AA solid medium, cryopreservation, and continuously suspending culture, were compared. The results indicated that the first method kept good suspensible property, had high regeneration rate, and had a little influence on activity of POD and SOD, even though the suspension cells grew on AA medium for 2–9 months. AA solid medium is ideal for the preservation of suspension cells in short-middle-term.

Key words: Rice, Suspension cell, Cryopreservation, Plant regeneration, POD, SOD

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