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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (03): 550-555.doi: 10.3724/SP.J.1006.2014.00550

• RESEARCH NOTES • Previous Articles     Next Articles

HC-Pro Gene Segment Mediated Hyper-Resistance to Turnip mosaic virus

YE Yan-Ying1.2,ZENG Gang1,CAO Ming-Qing1,MA Rong-Cai1,WU Cai-Jun2,*,YAO Lei1,*   

  1. 1 Beijing Ago-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2 College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2013-07-16 Revised:2013-12-10 Online:2014-03-12 Published:2014-01-16
  • Contact: 姚磊, E-mail: yaolei@baafs.net.cn; 吴才君, E-mail: wucj12@126.com

Abstract:

 TuMV (turnip mosaic virus) is one of the most major viruses in important economic crops, with broadest known hosts, especially for cruciferous plants. In order to get durable and stable high resistance to TuMV, in this study a 453 bp segment of TuMV HC-Pro gene was chosen as the target to construct pBBBTu-HC-Pro, and transformed into Arabidopsis thaliana, the TuMV nature host. Transgenic plants were inoculated with TuMV strain BJ-C4, which has high pathogenicity and is mainly epidemic in Beijing area. In 13 investigated transgenic lines, four lines showed hyper-resistance to TuMV. The resistance was enhanced 80%, and inherited to progenies. Semi-quantitative RT-PCR and quantitative PCR results revealed that the accumulation of virus was hardly to detect in highly resistant transgenic plants, indicating the resistance is prominent. The plasmid pBBBTu-HC-Pro should have broad applicability in crop breeding of TuMV resistance engineering.

Key words: Turnip mosaic virus, HC-Pro, Gene segment, RNAi, Resistance

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