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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (06): 881-888.doi: 10.3724/SP.J.1006.2015.00881

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Silence of Phytophthora infestans Induced by Plant-mediated RNA Interference in Potato

REN Qin1,WANG Ya-Jun1,GUO Zhi-Hong1,*,LI Ji-Ping2,XIE Zhong-Kui1,WANG Ruo-Yu1,WANG Li2,HUI Na-Na2   

  1. 1 Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
  • Received:2015-01-07 Revised:2015-03-19 Online:2015-06-12 Published:2015-03-30
  • Contact: 郭志鸿, E-mail: guozhhong@hotmail.com

Abstract:

Potato late blight caused by Phytophthora infestans is the most devastating disease in potato. The objective of the work was to test the efficiency of plant-meidated RNAi in silencing genes in P. infestans and to find a new way to breed potato resistant to late blight. Over-lap PCR was employed to amplify a fused-gene C1234 simultaneously homologous to four cellulose synthase genes in P. infenstans. Then, a plant expression vector containing inverted repeat of C1234 was constructed and transferred to Atlantic, a potato variety severely susceptible to late blight by agrobacteria-mediated genetic transformation. A hundred and twenty nine regenerated lines were confirmed to be transgenic plants with PCR and Southern blot. When detached leaves were inoculated with P. Infestans, 97 out of 129 transgenic lines delayed disease symptoms with smaller lesions and less hyphae compared with the wild type at six days after inoculation. Chlorotic spots disappeared on leaves from transgenic lines while appeared severely on leaves from the wild type at the same day. mRNA accumulation of the four cellulose synthase genes from P. infestans colonized on leaves from transgenic plants with delayed symptoms was significantly lower compared with the wild type with Real-time RT-PCR. The results demonstrated that dsRNA of C1234 generated in transgenic potato could induce homologous genes silence and delay the process of infection in intimately touched P. infestans.

Key words: Plant-mediated RNAi, Potato, Phytophthora infestans, Gene silence

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