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    12 June 2015, Volume 41 Issue 06
      Identification and Fine Mapping of Green-Revertible Chlorina Gene grc2 in Rice (Oryza sativa L.)
      TAN Yan-Ning,SUN Xue-Wu,YUAN Ding-Yang,SUN Zhi-Zhong,YU Dong,HE Qiang,DUAN Mei-Juan,DENG Hua-Feng,YUAN Long-Ping
      Acta Agron Sin. 2015, 41(06):  831-837.  doi:10.3724/SP.J.1006.2015.00831
      Abstract ( 705 )   RICH HTML    PDF (3235KB) ( 1216 )   Save
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      Green revertible leaf-color mutants are basical materials for studying the mechanism of chloroplast differentiation and development. We have obtained a green-revertible chlorina mutant named grc2 with every leaf greening independently, from an indica maintainer line T98B treated by 60Co-γ radiation. Each leaf of grc2 is initially chlorotic, and then turns green after growing about 10 days. The mutant grc2 showed a new pattern of virescence which refreshed green regardless of its plant growth stage. Compared with the wild type T98B, the total chlorophyll and chlorophyll b content reduced significantly in the yellowish leaves of grc2 and chloroplast remained in the etioplast stage, suggesting that grc2 would probably be an essential gene functioning in the development of young leaves. Genetic analysis revealed that, grc2 was controlled by a single recessive nuclear gene. The gene of grc2 was fine mapped between STS markers S254 and S258 with a physical interval of 31 kb on the short arm of chromosome 6, by using 960 F2 plants with mutant phenotype from a cross between grc2 and Nipponbare. This region contained five annotated genes that had not published. These results provides important information for studying in gene cloning and gene function of grc2.

      Identification and Gene Fine Mapping of White Panicle Mutant wp4 in Oryza sativa
      WANG Xiao-Wen,JIANG Yu-Dong,LIAO Hong-Xiang,YANG Bo,ZOU Shuai-Yu,ZHU Xiao-Yan,HE Guang-Hua,SANG Xian-Chun
      Acta Agron Sin. 2015, 41(06):  838-844.  doi:10.3724/SP.J.1006.2015.00838
      Abstract ( 585 )   RICH HTML    PDF (4440KB) ( 577 )   Save
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      At the flowering and filling stages, rice hull appears green color and possesses photosynthetic pigment. To clear the mechanism of chloroplast development in the panicle, we identified a novel white panicle mutant from the progeny of indica restorer line Jinhui 10 with seeds treated by EMS and termed it as wp4. The wp4 displayed green spike-stalks and milk-white hulls after the heading stage. The structures of chloroplast and thylakoid were severely destroyed and the contents of photosynthetic pigment decreased extremely significantly in the mutational hulls. Compared with the wild type, the wp4 displayed yellow green leaves and contained looser stromal lamellae. And the contents of chlorophyll a, b, and carotenoid were all declined while only the changing of chlorophyll a led to the significantly different level in statistics. Except for effective panicles number and seed setting rate, other detected agronomic characteristics decreased slightly but the changing did not come up to the statistically significant difference. Genetic analysis indicated that the white panicle of wp4 was controlled by a recessive nuclear gene and which was finally mapped on chromosome 8 with 79 kb physical distances according to 1200 mutational plants derived from the F2 generation of Xinong1A/wp4. The restricted region contained 14 annotated genes based on Rice Genome Annotation Project. These results provided a foundation for gene cloning and function analysis of the WP4. Meanwhile, the traits of wp4 could be available in rice breeding as a morphological marker.

      Expression Analysis of Genes Involved in Peanut Seed Dormancy Release (Arachis hypogaea L.)
      CHEN Jing,JIANG Ling,WANG Chun-Ming,HU Xiao-Hui,ZHAI Hu-Qu,WAN Jian-Min
      Acta Agron Sin. 2015, 41(06):  845-860.  doi:10.3724/SP.J.1006.2015.00845
      Abstract ( 615 )   RICH HTML    PDF (930KB) ( 1039 )   Save
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      Seed dormancy is one of important agronomic traits in peanut (Arachis hypogaea L.). Seed dormancy can be released with exogenous ethephon. To understand the molecular mechanisms of switches from dormancy to germination in peanut seeds underlying the role of ethephon, we preformed transcriptome analyses among imbibed dormant seeds as control and dormancy-released seeds (AE1, AE2, AE3) treated by 100 mg L–1 exogenous ethephon, and compared the expression of unigenes related to ABA, GA, ETH and auxin. The results showed that there were 15, 40, 60, and 56 unigenes associated with GA, ABA, ETH, and auxin respectively, which were significantly differentially expressed unigenes during the process from dormancy to germination. The results of Real-time RT-PCR showed that the expressions of AhNCED2 and AhCYP707A1 were induced distinctly by exogenous ethephon in seed dormancy released process. In dormant and non-dormant seed imbibition and germination processes, there were different roles between expresses of AhNCED2 and AhCYP707A1. AhNCED2 played a positive role in maintaining seed dormancy, while AhCYP707A1 played a positive role for seed dormancy breaking.

      Characterization of a Novel Spotted Leaf Mutant spl32 and Mapping of Spl32(t) Gene in Rice (Oryza sativa)
      ZHONG Zhen-Quan,LUO Wen-Long,LIU Yong-Zhu,WANG Hui,CHEN Zhi-Qiang,GUO Tao
      Acta Agron Sin. 2015, 41(06):  861-871.  doi:10.3724/SP.J.1006.2015.00861
      Abstract ( 843 )   RICH HTML    PDF (2815KB) ( 718 )   Save
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      A dominant spotted-leaf mutant of rice was isolated from F2 (Yuejingsimiao 2/H4) population. The mutant, designated as spl32 (spotted-leaf 32), initiated brown spots on leaf apex at the panicle differentiation period, and then gradually spread them to whole leaf and sheath. Trypan blue staining indicated that the formation of spots was not caused by cell death. Taken normal green leaf plants segregated from heterozygous F5 as control (CK), we found seeds per panicle and seed setting rate of spotted leaf plants were significantly lower than these of CK. After appearance of spots, the POD activity and MDA content of spl32 were significantly higher than these of CK, while photosynthetic pigment content in spl32 was reduced, without significant changes in chlorophyll fluorescence parameters. The resistance to rice bacterial blight in spl32 was greatly improved by inoculation of Xanthomonas oryzae pv. oryzae at heading period. The spotted-leaf trait of spl32 was verified to be controlled by a dominant gene that temporarily designated as Spl32(t). The novel rice spotted-leaf gene was mapped between markers Ind-c and RM206 on chromosome 11 with a F2 (02428/Spl32) population.

      Development and Identification of Wheat Lines H3714 and H4058 Resistant to Cereal Cyst Nematode
      SUN Xi-Ying,CUI Lei,SUN Lei,SUN Yan-Ling,QIU Dan,ZOU Jing-Wei,WU Xiao-Fei,WANG Xiao-Ming,LI Hong-Jie
      Acta Agron Sin. 2015, 41(06):  872-880.  doi:10.3724/SP.J.1006.2015.00872
      Abstract ( 578 )   RICH HTML    PDF (3067KB) ( 1005 )   Save
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      Cereal cyst nematode (CCN, Heterodera spp.) has occurred in major wheat (Triticum aestivum L.) producing regions in China. Developing wheat cultivars resistant to CCN is limited due to the shortage of effective sources of resistance. Madsen, a winter wheat cultivar released in the Pacific Northwest Region of the USA, is highly resistant to CCN populations from China. However, it is difficult to use this cultivar in breeding programs because of its late heading and maturity. In the present study, Chinese winter wheat cultivars Yannong 21 and Jimai 19 were crossed and backcrossed to Madsen, and lines H3714 and H4058 were developed from BC1F4 progenies. Tests under both natural infestation and artificial inoculation conditions indicated that resistance of H3714 and H4058 to the H. avenae Xingyang population (pathotype Ha43) and the H. filipjevi Xuchang population (pathotype Hfc-1) from Henan province was superior to that of Yannong 21 and Jimai 19. Results of the field test inoculated with a mixture of Bgt isolates demonstrated that both lines showed adult plant resistance to powdery mildew, and line H4058 was also resistant to different Bgt isolates at the seedling stage. The heading date of these lines was comparable to that of Yannong 21 and Jimai 19, and obviously earlier than that of Madsen. The fragments of chromosome 2NS from Aegilops ventricosa were detected in lines H3714 and H4058 using a pair of chromosome-specific primers VENTRIUP-LN2 and the molecular markers Vlr2.6-3′-Vlr2.4-5′ and VRGA-F11-VRGA-R5 that were specific for Vrga1D gene from Ae. ventricosa. Results of Illumina iSelect 90K SNP assay showed that the two lines differed in their chromosome constitutions. Two thirds of the polymorphic SNPs were identical and the remaining ones were different among 4918 polymorphic SNPs between the two sib lines. The newly developed wheat lines H3714 and H4058 can be used as sources of resistance in developing CCN-resistant wheat cultivars.

      Gene Silence of Phytophthora infestans Induced by Plant-mediated RNA Interference in Potato
      REN Qin,WANG Ya-Jun,GUO Zhi-Hong,LI Ji-Ping,XIE Zhong-Kui,WANG Ruo-Yu,WANG Li,HUI Na-Na
      Acta Agron Sin. 2015, 41(06):  881-888.  doi:10.3724/SP.J.1006.2015.00881
      Abstract ( 1004 )   RICH HTML    PDF (2184KB) ( 742 )   Save
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      Potato late blight caused by Phytophthora infestans is the most devastating disease in potato. The objective of the work was to test the efficiency of plant-meidated RNAi in silencing genes in P. infestans and to find a new way to breed potato resistant to late blight. Over-lap PCR was employed to amplify a fused-gene C1234 simultaneously homologous to four cellulose synthase genes in P. infenstans. Then, a plant expression vector containing inverted repeat of C1234 was constructed and transferred to Atlantic, a potato variety severely susceptible to late blight by agrobacteria-mediated genetic transformation. A hundred and twenty nine regenerated lines were confirmed to be transgenic plants with PCR and Southern blot. When detached leaves were inoculated with P. Infestans, 97 out of 129 transgenic lines delayed disease symptoms with smaller lesions and less hyphae compared with the wild type at six days after inoculation. Chlorotic spots disappeared on leaves from transgenic lines while appeared severely on leaves from the wild type at the same day. mRNA accumulation of the four cellulose synthase genes from P. infestans colonized on leaves from transgenic plants with delayed symptoms was significantly lower compared with the wild type with Real-time RT-PCR. The results demonstrated that dsRNA of C1234 generated in transgenic potato could induce homologous genes silence and delay the process of infection in intimately touched P. infestans.

      Epistatic and QTL × Environment Interaction Effects of QTLs for Leaf Traits and Leaf Chlorophyll Content in Soybean
      LIANG Hui-Zhen,YU Yong-Liang,YANG Hong-Qi,DONG Wei,XU Lan-Jie,NIU Yong-Guang,ZHANG Hai-Yang,LIU Xue-Yi,FANG Xuan-Jun
      Acta Agron Sin. 2015, 41(06):  889-899.  doi:10.3724/SP.J.1006.2015.00889
      Abstract ( 907 )   RICH HTML    PDF (1562KB) ( 1221 )   Save
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      A RIL population containing 447 lines, derived from a cross of cultivar Jingdou 23 × Huibuzhiheidou, as well as their parents were used to analyze inheritance and detect epistatic effects, and QTL × environment (QE) interactions related to leaf traits and leaf chlorophyll content (CC) in soybean using major gene plus polygene mixed inheritance analysis and composite interval mapping (QTL NETwork 2.0). The leaf traits including leaf length (LL), leaf width (LW), leaf stalk length (LSL) were evaluated in 2011, 2012, and 2013, as well as CC was detected on 1 August and 8 August, 2012, and on 2 August and 9 August, 2013. LL was found to be controlled by two pairs of additive-additive by additive epistatic hybrid main genes, LW was found to be controlled by three pairs of equivalent main genes, LSL was found to be controlled by four pairs of additive-additive by additive epistatic major genes, CC was controlled by four pairs of additive major genes. Ten QTLs for LL, LW, LSL, and CC were mapped on the linkage group (LG) A1, A2, C2, H_1, L, and O, separately. Of them two QTLs for LL were mapped on LG C2 and LG L, additive by additive epistatic effect and QE interactions. Three QTLs with additive effect and QE interactions associated with LW were mapped on LG A2, C2, and O. Two QTLs for LSL were mapped on LG L and O. Three QTLs for CC were mapped on LG A1, C2, and H_1. The genetic mechanism for leaf traits and leaf chlorophyll content is more complicated containing additive effect, additive × additive epistatic effect and QE interaction. It is important to consider not only to QTLs with major effects, but also to those with epistatic effects in soybean molecular marker-assisted breeding for stability of expression and inheritance of agronomic traits.

      Identification and Analysis of WRKY Transcription Factors in Triticum urartu
      MA Jian-Hui,ZHANG Dai-Jing,GAO Xiao-Long,SHAO Yun,JIANG Li-Na
      Acta Agron Sin. 2015, 41(06):  900-909.  doi:10.3724/SP.J.1006.2015.00900
      Abstract ( 766 )   RICH HTML    PDF (4519KB) ( 1393 )   Save
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      WRKY transcription factors have been found to be involved in the processes responding to various abiotic and biotic stresses in plants. The knowledge on WRKY transcription factors in Triticum urartu (AA) will facilitate the function study of WRKY transcription factors in hexaploid wheat (Triticum aestivum, AABBDD). In this study, 62 WRKY transcription factors with full length of coding sequence (CDS) were selected from Triticum urartu genome through bioinformatic analyses, in which 14 could be located on specific chromosomes and two pairs had been duplicated. These WRKY transcription factors were divided into eight subgroups by phylogenetic analysis and the exon–intron structure in individual subgroups was relatively conserved. Two WRKY transcription factors were selected for function validation, and their expressions consistently increased under diverse abiotic stresses by qRT-PCR assay.

      Analysis of Yield and Quality Traits and the Relationship between Them in Japonica Rice in the Northern China
      WANG Yan-Zheng,WANG Xiao-Jing,LI Yuan,XU Hai,WANG Jia-Yu,ZHAO Ming-Hui,TANG Liang,MA Dian-Rong,XU Zheng-Jin,CHEN Wen-Fu
      Acta Agron Sin. 2015, 41(06):  910-918.  doi:10.3724/SP.J.1006.2015.00910
      Abstract ( 700 )   RICH HTML    PDF (900KB) ( 1005 )   Save
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      As the scientific rice research develops and the standard of living improves, the demand for japonica rice has been consistently increasing and the planting area of japonica rice has been enlarged over times. Hence, a further investigation on the differences of yield and quality characters among different planting area and the relationships between yield and quality of japonica rice varieties can provide valuable guidance for rice breeding in northern China. In this study, we analyze the data collected from the regional rice tests of north China in 2011 and 2012. Based on the feature of the regional climates and the characteristics of the lines/varieties tested, we divided the lines/varieties tested we divided into five groups, including Huang-Huai japonica group, Beijing-Tianjin-Hebei japonica group, late-maturity mid-early japonica group, medium maturity mid-early japonica group, and early-maturity mid-early japonica group. Then, we analyzed the differences of the yield, yield components, quality traits and their relationships among the five groups. The yield of the mid-early japonica group with medium maturity was the highest; while the yield of the mid-early japonica rice with late-maturity was the lowest in the five groups. The mean rate of brown rice and the mean rate of head rice of the five groups were 83.66% and 66.95%, respectively. The rate of chalky rice and chalkiness degree were 30.26% and 3.08%, respectively. The yield significantly and positively correlated to the rate of brown rice, the rate of milled rice and the rate of head rice. The percentage of seed setting exhibited a significantly positive correlation to the ratio of brown rice, the ratio of milled rice, and the ratio of head rice, but a significantly negative correlation to the rate of chalky rice. Although the ratio of grain aspect showed a negative correlation to the ratio of brown rice and the ratio of head rice, the ratio of grain aspect was not closely related to yield. The density of seed setting had a significant and negative correlation to the number of panicle, but a significant and positive correlation to the number of grains per panicle, so that the density of seed setting was not closely related to yield. There were no significant correlations between the density of seed setting and the rate of seed setting, thousand grain weight, and chalkiness related traits. The results indicated that the yield can be increased at least to 9 t ha-1 without sacrificing the grain quality in northern China. It is feasible to improve quality based on maintaining high yield, or increase the yield based on maintaining high quality of rice, thus achieving a balance between the yield and quality at a higher level.

      Effects of Maize and Potato Intercropping on Rhizosphere Microbial Community Structure and Diversity
      QIN Xiao-Min,ZHENG Yi,TANG Li,LONG Guang-Qiang
      Acta Agron Sin. 2015, 41(06):  919-928.  doi:10.3724/SP.J.1006.2015.00919
      Abstract ( 714 )   RICH HTML    PDF (1103KB) ( 1308 )   Save
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      Field and pot experiments were conducted with three planting treatments (maize monocropping, potato monocropping, maize and potato intercropping) to investigate the changes of composition and function diversity of rhizosphere microbial community of maize and potato using Biolog technique. The results indicated that compared with monocropping, the average well color development (AWCD, utilization rate of 31 carbon sources) of rhizosphere microbes of intercropped maize and potato was increased by 17.36%, 7.38% and 3.76%, 32.21% respectively, among the intercropping and monocropping crops, the AWCD value of intercropped potato was the highest and the monocropped maize was the lowest. Functional diversity index of Shannon index (H), Simpson index (D), Evenness index (E) and Richness index (S) were higher in intercropping rhizosphere soil than these in the monocropping systems, but no significant difference. Among six types of carbon sources used by the rhizospheric microbes, carbohydrates, amino acids and carboxylic acids, had higher utilization rates with definite differences. Principal component analysis (PCA) and cluster analysis demonstrated that the amino acids, carbohydrates and carboxylic acids were the sensitive carbon sources for differentiating the changes of the microbial community induced by monocropping and intercropping. This study showed that intercropping can change the composition and enhance functional diversity of the microbial community in the rhizosphere of maize and potato.

      Effects of Autumn Straw Mulching on Physiological Characteristics and Water Use Efficiency in Winter Wheat Grown in Hilly Drought Region
      WU Xiao-Li,TANG Yong-Lu,LI Chao-Su,WU Chun,HUANG Gang
      Acta Agron Sin. 2015, 41(06):  929-937.  doi:10.3724/SP.J.1006.2015.00929
      Abstract ( 508 )   RICH HTML    PDF (486KB) ( 877 )   Save
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      Seasonal drought during winter and spring often occurs in southwest hilly area of China, which severely influences seedling standing and yield in winter wheat. In this study, we conducted a two-year field experiment in Jianyang, Sichuan province in the 2012–2013 (dry) and 2013–2014 (wet) growing seasons to explore the effects of straw mulching on physiological characteristics, water use efficiency (WUE), and grain yield of winter wheat. Four treatments were designed, namely non-mulching (CK), non-mulching plus two irrigations after sowing and at jointing stage (T1), straw mulching before sowing (T2), and straw mulching before sowing and during wheat growth (T3). In the dry year (2012–2013), the yields of T1, T2, and T3 were 4151, 3926, and 3603 kg ha-1, which were 41.96%, 34.25%, and 23.22% higher than those of CK, respectively, and the WUEs of T1, T2, and T3 increased by 27.23%, 29.59%, and 18.84%, respectively. However, in the wet year (2013–2014), the yield variation among treatments was slight. In the dry year, irrigation or straw mulching showed the effects on enhancing dry matter accumulation from sowing to anthesis and inhibiting SPAD attenuation of flag leaf and penultimate leaf after anthesis and straw mulching increased soil moisture content in pre-sowing and whole growing period. Compared with CK, T2 significantly increased root dry matter, root-to-shoot ratio, root length density, root dry matter density, and root surface area density in some critical growth stages, and highly enhanced root system in deep soil. According to correlation analysis, grain yield was positively correlated with dry matter accumulations in the periods of sowing–tillering, tillering–jointing, jointing–anthesis, and anthesis–maturity, SPAD values of flag and penultimate leaf after anthesis, and WUE. These results indicate that straw mulch before wheat sowing can maintain soil moisture, delay leaf senescence and increase grain yield in winter wheat.

      Effects of Paclobutrazol on Yield and Mechanical Harvest Characteristics of Winter Rapeseed with Direct Seeding Treatment
      YANG Yang,KUAI Jie,WU Lian-Rong,LIU Ting-Ting,SUN Yin-Yin,ZUO Qing-Tong,ZHOU Guang-Sheng,WU Jiang-Sheng
      Acta Agron Sin. 2015, 41(06):  938-945.  doi:10.3724/SP.J.1006.2015.00938
      Abstract ( 1111 )   RICH HTML    PDF (213KB) ( 864 )   Save
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      The aim of this research was to study the effects and mechanism of paclobutrazol (PP333) treatments on rapeseed yield and mechanical harvesting. PP333 was foliage sprayed with different concentrations at two stages (the closing of crop and the start of flower bud), on two varieties of rapeseed Yangguang 2009 and Fengyou 520. Our results demonstrated that PP333 treatment significantly increased the rapeseed lodging resistance, silique shattering resistance and yield. PP333 of 300 mg L–1 at the bud beginning more significantly enhanced lodging resistance and silique shattering resistance, whereas 150 mg L–1 PP333 at crop closing period more significantly increased yield of the two varieties. PP333 treatment reduced seed number per pod, while enhanced pods per plant, 1000-grain weight and yield. At the same time, PP333 treatment increased root crown diameter, root-top ratio (fresh) and snapping resistance, whereas it reduced plant height and culm lodging index, resulting in reduced angle of plant lodging, which indicates an improvement in the resistance of root and stem lodging. Silique shattering resistance was increased due to increased silique water content, silique dry weight and delayed pod maturity. In summary, the crop closing period and 150 mg L–1 PP333 are the best treatment time and concentration for significantly enhancing the abilities of lodging resistance, silique shattering resistance and yield, which could meet rapeseed mechanized production.

      Effects of High Temperature during Flowering Period on Seed Setting Rate and Its Relationship with Sink to Source Ratios and Flowering Habit of Mid-season Hybrid Rice
      XU Fu-Xian,ZHANG Lin,XIONG Hong,ZHU Yong-Chuan,LIU Mao,JIANG Peng,GUO Xiao-Yi
      Acta Agron Sin. 2015, 41(06):  946-955.  doi:10.3724/SP.J.1006.2015.00946
      Abstract ( 664 )   RICH HTML    PDF (345KB) ( 785 )   Save
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      From 2011 to 2013, under the condition of high temperature injury during heading stage designed by applying different sowing dates in the field and controlling the temperature in intelligent artificial climate chamber, the relationship high temperature resistance index (HTRI) with sink to source ratio and flowering habit in mid-season hybrid rice was studied with 50 hybrid combinations as tested materials. By using variance, correlation and regression analyses, the results showed that there were significant differences in HTRI among hybrid combinations. Ten of the combinations were tested to show a higher levels of seed setting rate and HIRI during flowering stage, including Mianxiangyou 576, Rongdaoyou 415, Xiangluyou 727, Mianyou 5240, Chuanguyou 202, Chuanyou 6203, Gangyou 169, Neixiangyou 5828, Chuanguyou 7329, and Jiangyou 126. There existed a significant relation between HTRI and carbohydrate supply level during flowering stage. The smaller the number of effective panicles of hybrid combinations was, the higher the spikelets per panicle and grain-leaf ratio would be, namely, the less the occupancy of photosynthetic source per spikelets would be. As a consequence, significantly negative correlation were observed between HTRI and grain-leaf ratio during flowering stage. The flowering ratio from 9:30 to 11:00 and chlorophyll content of leaf had a significantly positive correlation with HTRI respectively. Choosing the hybrid combinations with a larger number of effective panicles and early intensive anthesis and raising the level of chlorophyll content of leaf are the vital ways to enhance HTRI during heading stage.

      Effect of Salicylic Acid on Growth and Nutrient Uptake in Hydroponic Tobacco under Cu Stress
      XU Gen-Di,GE Shu-Fang,ZAHNG Yi,WU Yu-Huang,MEI Xiao-Man,LIU Peng
      Acta Agron Sin. 2015, 41(06):  956-962.  doi:10.3724/SP.J.1006.2015.00956
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      A hydroponic experiment was conducted to study the effects from treatmeats including CK ( Cu2+ 0 mg L–1, SA 0 μmol L–1 as control), T1 (Cu2+ 4 mg L–1, SA 0 μmol L–1), T2 (Cu2+ 4 mg L–1, SA 100 μmol L-1), T3 (Cu2+ 4 mg L–1, SA 300 μmol L–1), T4 (Cu2+ 4 mg L–1, SA 500 μmol L–1)on growth characteristics, elements absorption and accumulation in two tobacco cultivars, N. tabacum cv. W38 (Cu resistant genotype) and N. benthamiana (Cu sensitive genotype) under copper stress. The result showed that the growth of two tobacco cultivars under 4 mg L–1 Cu stress for 15 days was inhibited to some extent, meanwhile, the content of Cu in their organs was increased while the absorptions of K, Ca, Mg, Fe, Zn, B, and Mn were inhibited. The addition of exogenous SA could alleviate the inhibition of plant height, root length and fresh weight, and reduce Cu content of tobacco, but increase the absorption of nutritional elements K, Ca, Mg, Fe, and Mn. Compared with treatment T1, contents of Cu in root, stem and leaf were increased by 25.05%, 39.78%, 22.91% in N. benthamiana, and by 23.27%, 37.30%, 28.88% in N. tabacum cv.W38, respectively. In conclusion, appropriate concentration of SA promotes the growth and elements absorption in tobacco under copper stress. In this experiment, 300 μmol L–1SA could significantly alleviate copper inhibition to tobacco.

      Identification of Drought Resistence at Seedlings Stage in Common Bean (Phaseolus vulgaris L.) Varieties
      LI Long,WANG Lan-Fen,WU Jing,JING Rui-Lian,WANG Shu-Min*
      Acta Agron Sin. 2015, 41(06):  963-971.  doi:10.3724/SP.J.1006.2015.00963
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      Eleven physiological indices of different common bean varieties were measured under the conditions of irrigation and repeated drought in pot, drought resistance evaluation indices at seedling stage were selected by using grey relational thoery, the drought resistance of each germplasm was comprehensively evaluated according to weighted drought-resistance index and drought-resistance measuration value (D value) and graded by fuzzy cluster analysis. The results showed that the association degree between different indices and comprehensive drought resistance indices was ranked as follows: leaf relative water content (0.7726), Fv/Fm (0.7607), chlorophyll content (0.7435), survival rate (0.7341), biomass (0.7329), shoot dry weight (0.7314), root dry weight (0.7192), stomatal conductance (0.7159), Root/Shoot ratio (0.7092), plant height (0.7086), leaf area (0.6910). The evaluation results based on weighted drought-resistance index and D value had some differences, but the drought resistance rank was essentially the same. According to D value, tested materials were divided into four groups including high resistance(10%), moderate resistance(6%), susceptible(58%) and high susceptible(26%). In conclusion, ten indices including leaf relative water content, Fv/Fm, chlorophyll content and so on can be used to evaluate the drought resistance at seedling stage in common bean; combining weighted drought-resistance index and D value can improve the reliability of identification results. Among the 50 tested varieties, Baijindelidou, Yuejindou, Tuzutui, Yuanbaicaidou and 260205 have the better drought resistance.

      Interaction Analysis of Brassica oleracea L. BoExo70A1 with BoSEC3 and BoExo84 Proteins by Using Yeast Two-hybird System
      GAO Qi-Guo,LIU Yu-Dong,PU Quan-Ming,ZHANG Lin-Cheng,ZHU Li-Quan,WANG Xiao-Jia
      Acta Agron Sin. 2015, 41(06):  972-978.  doi:10.3724/SP.J.1006.2015.00972
      Abstract ( 670 )   RICH HTML    PDF (1858KB) ( 730 )   Save
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      Exo70A1 is necessary for Brassica stigma accepting the compatible pollen, and may play an important role in regulating the movement of water from the dry stigma to the pollen grain and compatible pollen hydration. Here, the coding sequences of BoExo70A1, BoSEC3, BoSEC10, BoSEC15, and BoExo84 genes were amplified from the stigma cDNA of the highly self-incompatible Brassica oleracea L. line A1. Sequence analysis showed that the cDNA sequences of BoExo70A1, BoSEC3, BoSEC10, BoSEC15, and BoExo84 genes were highly homologous to those of AtExo70A1, AtSEC3A, AtSEC10, AtSEC15B, and AtExo84B genes respectively. No signal peptide was found in their deduced protein sequences. BoSEC3 protein contained an EF-hand calcium-binding domain. The BoExo84 protein was divided into two fragments, BoExo84-N and BoExo84-C, and then their encoding sequences were subcloned into pGADT7 vector together with those of BoSEC3, BoSEC10, and BoSEC15 proteins respectively, whereas the encoding sequence of BoExo70A1 protein was subcloned into pGBKT7 vector for analysis of the proteins interaction by yeast two-hybid system. The results showed that BoExo70A1 protein interacted with BoSEC3 or BoExo84-N protein, but not with BoSEC10 or BoSEC15 protein.

      Construction of Genetic Map and QTL Analysis for Mainstem Height and Total Branch Number in Peanut (Arachis hypogaea L.)
      CHENG Liang-Qiang,TANG Mei,REN Xiao-Ping,HUANG Li,CHEN Wei-Gang,LI Zhen-Dong,ZHOU Xiao-Jing,CHEN Yu-Ning,LIAO Bo-Shou,JIANG Hui-Fang*
      Acta Agron Sin. 2015, 41(06):  979-987.  doi:10.3724/SP.J.1006.2015.00979
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      Peanut is an allotetraploid crop with a large genome. The construction of genetic linkage map and QTL mapping of related traits has little progress for peanut. In the present study, a genetic map consisting of 20 linkage groups was constructed with 234 SSR markers based on 2653 published SSR markers by using the F2 population derived from the cross between Fuchuan Dahuasheng and ICG6375. The genetic map covers 1683.43 cM, and the length of each linkage group varies from 36.11 to 131.48 cM, the number of markers in each linkage group varies from 6 to 15, with an average distance of 7.19 cM. Combining with the data of main stem height and number of total branches of F3 population in the environments of Wuhan and Yangluo, we performed QTL mapping and genetic effects analysis of QTLs by software WinQTLCart 2.5 using CIM (Composite Interval Mapping) method. As a result, 17 QTLs related to main stem height and number of total branches on eight linkage groups were detected with contribution percentage of 0.10%–10.22%. Comparing the QTLs detected in the environments of Wuhan and Yangluo, qMHA061.1 and qMHA062.1 were in the same linkage region of markers TC1A2–AHGS0153 of linkage group LG06 with contribution percentage of 5.49%–8.95%, qMHA061.2 and qMHA062.2 were between the markers AHGS1375 and PM377 on linkage group LG06 with contribution ratio of 2.93%–5.83%, qMHA092.2 and qMHA091.1 were in the same linkage region of the markers GM2839–EM87 in linkage group LG09 with contribution percentage of 0.53%–9.43%. The QTLs repeatedly detected are important for molecular breeding of peanut.

the Crop Science Society of China
the Institute of Crop Science, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Chen Xiao-ya Yang Jian-chang Zhang Xian-long Wang Jian-kang Xu Ming-liang Liu Chun-ming Wang Dao-wen Sun Chuan-qing Ding Yan-feng Jin Wei-wei Chu Cheng-cai Cheng Wei-hong
Director of the editorial department:
Cheng Wei-hong
CN 11-1809/S
ISSN 0496-3490
Post subscription code: 82-336

  • Started in 2013
  • Covered by SCIE
  • Open access in ScienceDirect

Editor in chief: Wan Jian-min
CN 10-1112/S
ISSN 2095-5421, 2214-5141(online)
Online published:
Submission: https://www.editorialmanager.com/cj/
E-mail: cropjournal@caas.cn
Tel: 8610-82108548