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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (06): 838-844.doi: 10.3724/SP.J.1006.2015.00838

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Gene Fine Mapping of White Panicle Mutant wp4 in Oryza sativa

WANG Xiao-Wen,JIANG Yu-Dong,LIAO Hong-Xiang,YANG Bo,ZOU Shuai-Yu,ZHU Xiao-Yan,HE Guang-Hua*,SANG Xian-Chun*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2014-11-24 Revised:2015-03-19 Online:2015-06-12 Published:2015-04-17
  • Contact: 桑贤春, E-mail: sangxianchun@163.com; 何光华, E-mail: hegh@swu.edu.cn E-mail:xwwang78@126.com

Abstract:

At the flowering and filling stages, rice hull appears green color and possesses photosynthetic pigment. To clear the mechanism of chloroplast development in the panicle, we identified a novel white panicle mutant from the progeny of indica restorer line Jinhui 10 with seeds treated by EMS and termed it as wp4. The wp4 displayed green spike-stalks and milk-white hulls after the heading stage. The structures of chloroplast and thylakoid were severely destroyed and the contents of photosynthetic pigment decreased extremely significantly in the mutational hulls. Compared with the wild type, the wp4 displayed yellow green leaves and contained looser stromal lamellae. And the contents of chlorophyll a, b, and carotenoid were all declined while only the changing of chlorophyll a led to the significantly different level in statistics. Except for effective panicles number and seed setting rate, other detected agronomic characteristics decreased slightly but the changing did not come up to the statistically significant difference. Genetic analysis indicated that the white panicle of wp4 was controlled by a recessive nuclear gene and which was finally mapped on chromosome 8 with 79 kb physical distances according to 1200 mutational plants derived from the F2 generation of Xinong1A/wp4. The restricted region contained 14 annotated genes based on Rice Genome Annotation Project. These results provided a foundation for gene cloning and function analysis of the WP4. Meanwhile, the traits of wp4 could be available in rice breeding as a morphological marker.

Key words: Rice (Oryza sativa)White panicle, Yellow green leaf, Gene mapping, Leaf color marker

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