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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (07): 1017-1026.doi: 10.3724/SP.J.1006.2015.01017

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Analysis of Nonspecific Phospholipase C gene SiNPC4 in Foxtail Millet (Setaria italic)

HU Li-Qin1,XUE Fei-Yang1,2,LI Wei-Wei1,3,WANG Er-Hui1,2,XU Zhao-Shi1,LI Lian-Cheng1,ZHOU Yong-Bin1,2,JIA Guan-Qing1,DIAO Xian-Min1,MA You-Zhi1,CHEN Ming1,*   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China; 2 College of Agronomy, Northwest A&F University / State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, China; 3 Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province / College of Life Science and Technology, Harbin Normal University, Harbin 150025, China?
  • Received:2015-01-14 Revised:2015-04-02 Online:2015-07-12 Published:2015-04-17
  • Contact: 陈明, E-mail: chenming02@caas.cn, Tel: 13683360891 E-mail:huliqin_2012@163.com

Abstract:

Nonspecific phospholipase C (NPC) catalyzes the hydrolysis of phosphatidylcholine or phosphatidylethanolamine to generate diacylglycerol (DAG), which is an important second messenger in cells. NPC family plays a key role in response to some stresses such as drought, high salinity and phosphorus deficiency, and is involved in some hormone signaling pathways such as abscisic acid (ABA) and brassinolide (BL). Taking foxtail millet as material, we cloned a novel NPC gene named SiNPC4 by sequence alignment. This gene was detected to be located on chromosome 8. The full length of SiNPC4 was 2877 bp with three exons and two introns encoding 512 amino acid residues and the protein molecular weight was 56.77 kD. Phylogenetic analysis of NPC protein sequences indicated that SiNPC4 distributed to the third subfamily. The predicted protein structure of the gene contained conserved phosphoesterase domain and four motifs. The protein subcellular localization analysis revealed that SiNPC4 was localized in cytomembrane, cytoplasm, and nucleus. The gene expression profile results indicated SiNPC4 mainly expressed in root and was induced by drought, salt, cold, dark, ABA, BL, methyl jasmonate (MeJA), and gibberellic acid (GA) treatments. Arabidopsis carrying SiNPC4 decreased the sensitivity to ABA and BL compared with WT. Thus, we deduced that SiNPC4 may act as a negative regulator in ABA and BL signaling pathways. Besides, there were no significant difference in growth between transgenic and wild type plants under drought and high salinity treatments.

Key words: Foxtail millet, SiNPC4, Abiotic stress, ABA, BL

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