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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (10): 1510-1518.doi: 10.3724/SP.J.1006.2015.01510

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Mapping for Kernel Related Traits Based on a High-Density Genetic Map

QIN Wei-Wei,LI Yong-Xiang,LI Chun-Hui,CHEN Lin,WU Xun,BAI Na,SHI Yun-Su,SONG Yan-Chun,ZHANG Deng-Feng,WANG Tian-Yu*,LI Yu*   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-03-09 Revised:2015-06-01 Online:2015-10-12 Published:2015-07-01
  • Contact: 王天宇,E-mail: wangtianyu@caas.cn;黎裕,E-mail: liyu03@caas.cn E-mail:13811531756@139.com

Abstract:

Kernel size and weight are major determinants of grain yield. For understanding molecular mechanisms of kernel related traits, a recombinant inbred line (RIL) mapping population including 130 families was developed from the cross of two maize elite inbreds, Huangzaosi (HZS) and Mo17. By using the approach of GBS (genotyping-by-sequencing), the high-density genetic map with 1262 bin markers was constructed. QTLs for kernel related traits were identified by stepwise regression (RSTEP-LRT) using Windows QTL ICI-Mapping software in five environments. In total, 30 QTLs were detected in single environment and 11 QTLs were detected in joint environment. The kernel length major QTL qklen1 and the length/width major QTL qklw1 were found in the adjoining regions with a strong genetic effect in three environments. QTL qklen1 was located in a region of 210–212 Mb on chromosome 1 with explained 22.60% of phenotypic variance, and qklw1 was located in a region of 207–208 Mb on chromosome 1 with explained 26.79% of phenotypic variance. In addition, for further verification, another introgression population of BC3F1 was developed with Mo17 as the donor parent and HZS as the recurrent parent. The result of the single marker analysis suggested that qklen1 and qklw1 also had a significantly genetic effect, which is similar to that in the RIL population. The present study provides a good basis for studying genetic mechanism and molecular marker assisted selection for the improvement of kernel related traits in maize.

Key words: Maize, Kernel related trait, QTL, High-density genetic map

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