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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (10): 1519-1528.doi: 10.3724/SP.J.1006.2015.01519

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization and Gene Mapping of a Spotted-leaf Mutant spl21 in Rice (Oryza sativa L.)

SONG Li-Xin1,2,HUANG Qi-Na1,FENG Bao-Hua1,SHI Yong-Feng1,ZHANG Xiao-Bo1,XU Xia1,WANG Hui-Mei1,LI Xiao-Hong1,ZHAO Bao-Hua2,*,WU Jian-Li1,*   

  1. 1 State Key Laboratory of Rice Biology / Chinese National Center for Rice Improvement / China National Rice Research Institute, Hangzhou 310006, China; 2 College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2015-03-12 Revised:2015-06-01 Online:2015-10-12 Published:2015-06-03
  • Contact: 赵宝华, E-mail: zhaobaohua86178@sohu.com; 吴建利, E-mail: beishangd@163.com

Abstract:

The rice spotted-leaf 21 mutant (spl21) was isolated from a diepoxybutane-induced IR64 mutant bank. Under field conditions, the red-brown spots appeared on the leaves of mutant seedlings in two weeks after sowing. Subsequently, a portion of spots merged and the leaf tips became yellowish, wilted and spread downwards along both edges of the leaf blade leading to the death of the whole leaf blade when the symptom was severe. Accumulation of H2O2 was detected in and around the spots. Major agronomic traits including plant height, length of panicle, number of panicles, number of filled grains, seed setting-rate and 1000-grain weight were markedly affected in the mutant. The contents of chlorophyll a, b, carotenoid and photosynthetic parameters were significantly reduced in the mutant as compared with the wild type. Furthermore, the activities of CAT, SOD, APX and soluble protein contents were significantly lower than those of the wild type while the activity of POD was apparently higher than that of the wild type. The mutant trait was controlled by a single recessive nuclear gene, tentatively termed spl21(t), located on the long arm of chromosome 12. The population and data achieved in the present study would facilitate the isolation and functional analysis of spl21(t).

Key words: Rice, Spotted-leaf mutant, Hydrogen peroxide, Photosynthetic pigment, Gene mapping

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