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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (10): 1529-1536.doi: 10.3724/SP.J.1006.2015.01529

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Verification of a CAPS Marker Linked to Tuber Shape Gene in Potato

ZHU Wen-Wen1,XU Jian-Fei1,LI Guang-Cun1,DUAN Shao-Guang1,LIU Jie1,BIAN Chun-Song1,PANG Wan-Fu1,Walter De Jong2,JIN Li-Ping1,*   

  1. 1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture, Beijing 100081, China; 2 Department of Plant Breeding and Genetics, Cornell University, Ithaca 14853, NY, USA
  • Received:2015-03-20 Revised:2015-07-10 Online:2015-10-12 Published:2015-07-15

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Abstract:

Tuber shape is one of the most important traits for potato cultivars especially for the processing varieties. Previous study shows that a single gene Ro on chromosome 10 controls most variation for round versus long tuber shape. In this study, based on the potato genome sequence information and bulked segregation analysis strategy, we developed a CAPS marker 1137-CAPSVI using the diploid potato genotypes 320-02, 10618-01 and their 213 F1 tuber shape segregated population. This marker was verified in 53 tetraploid breeding lines with diverse tuber shapes. The results showed that the consistency rate between the marker and tuber shape was 83.02% and the accuracy of the CAPS marker 1137-CAPSVI for selecting round tuber shape was up to 91.42%, and the further Pearson correlation analysis result showed that the marker was significantly correlated with tuber shape. The CAPS marker provides a basis for molecular marker-assisted selection in potato breeding especially for processing variety.

Key words: Potato, Tuber shape, Marker development, Molecular marker-assisted breeding

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