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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1343-1352.doi: 10.3724/SP.J.1006.2015.01343

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Analysis of Glycine soja Bicarbonate Stress Responsive Gene GsMIPS2

CHEN Chen1, SUN Xiao-Li2, LIU Ai-Lin1, DUAN-MU Hui-Zi1, YU Yang1, XIAO Jia-Lei1, ZHU Yan-Ming1, *   

  1. 1 College of Life Sciences, Northeast Agricultural University, Harbin 150030, China; 2 College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2015-01-20 Online:2015-09-12 Published:2015-09-12

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Abstract: To identify the key functional genes in response to bicarbonate stress in Glycine soja L. G07256, we constructed a gene expression profile under bicarbonate treatment using high throughput RNA-seq data, from which we selected a myo-inositol- 1-phosphate synthase gene whose expression was significantly induced by bicarbonate stress. By homology-based cloning, we acquired its full length cDNA and termed as GsMIPS2. The results of quantitative real-time PCR demonstrated the bicarbonate stress induced expression of GsMIPS2 and its tissue expression specificity. We also verified the function of GsMIPS2 in bicarbonate responses by using the GsMIPS2 transgenic Arabidopsis combined with the T-DNA insertion line atmips2. We revealed that the germination rate of GsMIPS2 overexpression lines was significantly higher, while that of atmips2 mutant line was much lower than that of wild type under bicarbonate stress. These results indicate the positive role of GsMIPS2 in plant bicarbonate stress responses.

Key words: Glycine soja, GsMIPS2, Myo-inositol-1-phosphate synthase, Bicarbonate stress

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