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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (02): 243-254.doi: 10.3724/SP.J.1006.2016.00243

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Comparative Proteomics Analysis of R. glutinosa Tuber Root in Response to Consecutive Monoculture

WU Lin-Kun1,2,CHEN Jun1,2,WU Hong-Miao1,2,WANG Juan-Ying1,2,QIN Xian-Jin1,2,ZHANG Zhong-Yi2,LIN Wen-Xiong1,2,*   

  1. 1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring / Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2015-05-27 Revised:2015-11-20 Online:2016-02-12 Published:2015-12-07
  • Contact: 林文雄, E-mail: lwx@fafu.edu.cn E-mail:wulinkun619@163.com
  • Supported by:

    This study was supported by the National Science Foundation of China (81303170, U1205021) and the Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University (324-1122yb005).

Abstract:

Rehmannia glutinosa L. is widely applied in Chinese medicine. However, consecutive monocropping of this plant results in serious decline in both biomass and quality of underground tubers, with poor field performance and insufficient resistance to disease and pest. In the present study, the tuber roots from the newly planted (NP) and consecutively monocropped (SM) R. glutinosa were used through comparative proteomics analysis to study the response of R. glutinosa to consecutive monocropping and the underlying mechanisms of replanting disease. Comparative proteomics analysis showed that these proteins involved in important physiological processes and biosynthese of main components in tuber roots were significantly down-regulated under consecutive monocropping regime.It was also found that chaperonins related to protein folding was down-expressed with the extended monocropping. However, these proteins related to stress response/defense such as pathogenesis-related protein 10, cytochrome P450 and Type IIIa membrane protein cp-wap13 were up-regulated in consecutively monocropped R. glutinosa. Quantitative analysis by qRT-PCR confirmed the up-regulation of PR-10 responding to consecutive monocropping or the infection of pathogenic Fusarium oxysporum. Moreover, PR-10 was gradually up-regulated with the increasing days of infection. In conclusion, consecutive monocropping of R. glutinosa greatly affects the expression profile of proteome in tuber roots. These abnormally expressed proteins might lead to the metabolic disturbances and low energy production. Moreover, the limited energy is applied to resist the external environmental stresses, resulting in significant decline in the growth of tuber roots and the accumulation of active ingredients.

Key words: Rehmannia glutinosa, Consecutive monocropping problem, Comparative proteomics, Pathogenesis-related protein, Stress response

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