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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 734-742.doi: 10.3724/SP.J.1006.2016.00734

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Mechanism Regulating Light-induced Mesocotyl Elongation by Polyamine Oxidase (PAO) in Maize

ZHANG Tong-Zhen1,**,LI Yong-Sheng1,**,LI Yue2,YAO Hai-Mei1,ZHAO Juan1,WANG Chan1,ZHAO Yang1,WANG Han-Ning1,FANG Yong-Feng1,*,Hu Jin3,*   

  1. 1Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement / Gansu Provincial Key Laboratory of Aridland Crop Science / Gansu Agricultural University,Lanzhou 730070, China; 2Institute of Crop Science, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3 College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China
  • Received:2015-09-16 Revised:2016-03-02 Online:2016-05-12 Published:2016-03-11
  • Contact: 方永丰, E-mail: fangyf@gsau.edu.cn;胡晋, E-mail: jhu@zju.edu.cn E-mail:294301204@qq.com
  • Supported by:

    This study was supported by National Basic Research Program of China (973 Program, 2012CB722902),the National Natural Science Foundation of China (31371708,31201279), and Research Program Sponsored by Gansu Provincial Key Laboratory of Aridland Crop Science (GSCS201210).

Abstract:

In this study, maize inbred line PH4CV was culturedunder in MS mediumunder both dark and light conditionsto investigate the relationship of length of mesocotyl with activity of Polyamine Oxidase(PAO), activity of peroxidase (POD), contents of H2O2 ang lignin. In addition, after treated with5 mmolL-1 2-hydroxyethylhydrazine (2-HEH) and 5 mmolL-1 N, N’-Dimethylthiourea (DMTU), the origin and accumulation site of H2O2 in mesocotyl elongationwere assessed by using histochemical stainingmethod and the effect of lighton expression of ZmPAO gene was evaluated. The results indicated that the elongation of mesocotyl was significantly inhibited while the contents of H2O2 and lignin and the activities of POD and PAO were enhanced by light. Correlation analysis showed that the length of mesocotylwas negatively correlated with the PAO activity, H2O2 content, POD activity, and lignin content, and the PAO activity was positively correlated with the H2O2 content, the POD activity, and the lignin content. The assay with 2-HEH and DMTU, the microgram of epidermal cells, and histochemical localizationof H2O2 revealed that H2O2 participated in mesocotyl elongation, in which PAO catalyzedpolyamines (PAs)degradation to produce H2O2resulting in the inhibition of mesocotyl elongation.Results of qRT-PCR revealed that the expression level of ZmPAO was relatively stable in dark and rose rapidly of0.5 hour after exposing to light, with a maximum value after three hours of light treatment, then declined gradually, and finally showed a steady level after ten hours. This study suggests that the activity of PAO can be promoted by light treatment, and initiate the PAs-mediated induction of H2O2, resulting in the oxidation of lignin monomers on cell wall by POD. The produced free radicalsare then transformed into lignin in a polyforming process which marked the cell wall hardened and cell elongationinhibited . This study may provide a theoretical basis for understanding the physiological mechanism underlying the PAO-mediated mesocotyl elongation and gain insights into the response of maize mesocotyl to the light stress.

Key words: Maize, Light, Mesocotyl elongation, Polyamine oxidase, Expression analysis

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