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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 1067-1073.doi: 10.3724/SP.J.1006.2016.01067

• RESEARCH NOTES • Previous Articles     Next Articles

Expression Patterns of MADS-box Genes Related to Flower Development of Wheat

LI Hai-Feng1,2,*,HAN Ying1,LIU Meng-Jia1,WANG Bing-Hua1,SU Ya-Li1,SUN Qi-Xin1,*   

  1. 1 State Key Laboratory of Crop Stress Biology for Arid Areas / College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Xinjiang Agriculture Vocational Technology College, Changji 831100, China
  • Received:2015-11-24 Revised:2016-03-14 Online:2016-07-12 Published:2016-05-09
  • Contact: 李海峰, E-mail: lhf@nwsuaf.edu.cn; 孙其信, E-mail: qxsun@cau.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31571657), the Fundamental Research Foundation for the Central Universities (2014ZZ009), and the Foundation of Xinjiang Agriculture Vocational Technology College (XJNZYKJ201501).


 The objective of this study was to elucidate the molecular mechanism of wheat (Triticum aestivum L.) flower development. According to the phylogenetic tree of MADS- box genes from different species, we found that wheat contained all kinds of genes involved in the ABCDE model for flower development. The expression patterns of A-, B-, C-, D-, and E-class genes were analyzed by semi-quantitative and quantitative RT-PCR (qRT-PCR). Wheat AP1/FUL gene TaFUL (A-class) was expressed in all floral organs with the highest expression level in lemmas and paleas. Genes TaAP3 (B-class), TaAG (C-class) showed conservative expression patterns in specific organs, i.e., TaAP3 was expressed in lodicules and stamens whereas TaAG was expressed in stamens and pistils. The OsMADS13 homologous gene in wheat (D-class) was expressed in both pistils and lodicules, suggesting its function in lodicule and ovule development simultaneously. Gene TaSEP (E-class) was expressed in paleas and the inner-three whorls except for lemmas and glumes. LHS1 is a grass-specific gene family and belongs to E-class. The expression of TaLHS1 was detected in lemmas, paleas, and glumes of wheat. TaDL, the homologous gene of rice DROOPING LEAF (DL) controlling carpel development, was expressed in glumes, lemmas and carpels. These results suggest a conservative molecular mechanism for flower development in wheat, but some genes may have diversified functions due to evolution. The expression evidence of TaDL and TaLHS1 in glumes, in combination with the morphology and structure analyses of glume, lemma and palea, implied that lemma and glume might originate from the same organ in wheat.

Key words: Wheat, Flower development, MADS- box gene, Expression pattern

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