Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 1067-1073.doi: 10.3724/SP.J.1006.2016.01067

• RESEARCH NOTES • Previous Articles     Next Articles

Expression Patterns of MADS-box Genes Related to Flower Development of Wheat

LI Hai-Feng1,2,*,HAN Ying1,LIU Meng-Jia1,WANG Bing-Hua1,SU Ya-Li1,SUN Qi-Xin1,*   

  1. 1 State Key Laboratory of Crop Stress Biology for Arid Areas / College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Xinjiang Agriculture Vocational Technology College, Changji 831100, China
  • Received:2015-11-24 Revised:2016-03-14 Online:2016-07-12 Published:2016-05-09
  • Contact: 李海峰, E-mail: lhf@nwsuaf.edu.cn; 孙其信, E-mail: qxsun@cau.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31571657), the Fundamental Research Foundation for the Central Universities (2014ZZ009), and the Foundation of Xinjiang Agriculture Vocational Technology College (XJNZYKJ201501).

Abstract:

 The objective of this study was to elucidate the molecular mechanism of wheat (Triticum aestivum L.) flower development. According to the phylogenetic tree of MADS- box genes from different species, we found that wheat contained all kinds of genes involved in the ABCDE model for flower development. The expression patterns of A-, B-, C-, D-, and E-class genes were analyzed by semi-quantitative and quantitative RT-PCR (qRT-PCR). Wheat AP1/FUL gene TaFUL (A-class) was expressed in all floral organs with the highest expression level in lemmas and paleas. Genes TaAP3 (B-class), TaAG (C-class) showed conservative expression patterns in specific organs, i.e., TaAP3 was expressed in lodicules and stamens whereas TaAG was expressed in stamens and pistils. The OsMADS13 homologous gene in wheat (D-class) was expressed in both pistils and lodicules, suggesting its function in lodicule and ovule development simultaneously. Gene TaSEP (E-class) was expressed in paleas and the inner-three whorls except for lemmas and glumes. LHS1 is a grass-specific gene family and belongs to E-class. The expression of TaLHS1 was detected in lemmas, paleas, and glumes of wheat. TaDL, the homologous gene of rice DROOPING LEAF (DL) controlling carpel development, was expressed in glumes, lemmas and carpels. These results suggest a conservative molecular mechanism for flower development in wheat, but some genes may have diversified functions due to evolution. The expression evidence of TaDL and TaLHS1 in glumes, in combination with the morphology and structure analyses of glume, lemma and palea, implied that lemma and glume might originate from the same organ in wheat.

Key words: Wheat, Flower development, MADS- box gene, Expression pattern

[1] Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 199, 353: 31–37
[2] Pelaz S, Ditta G S, Baumann E, Wisman E, Yanofsky M F. B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature, 2000, 405: 200–203
[3] Theissen G. Development of floral organ identity: stories from the MADS house. Curr Opin Plant Biol, 2001, 4: 75–85
[4] Wang K, Tang D, Hong L, Xu W, Huang J, Li M, Gu M, Xue Y, Cheng Z. DEP and AFO regulate reproductive habit in rice. PLoS Genet, 2010, 6: e1000818
[5] Kobayashi K, Yasuno N, Sato Y, Yoda M, Yamazaki R, Kimizu M, Yoshida H, Nagamura Y, Kyozuka J. Inflorescence meristem identity in rice is specified by overlapping functions of three AP1/FUL-like MAD-box genes and PAP2, a SEPALLATA MADS-box gene. Plant Cell, 2012, 24: 1848–1859
[6] Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y. SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130: 705–718
[7] Yamaguchi T, Lee DY, Miyao A, Hirochika H, An G, Hirano H Y. Functional diversification of the two C-class MADS-box genes OsMADS3 and OsMADS58 in Oryza sativa. Plant Cell, 2006, 18: 15–28
[8] Dreni L, Jacchia S, Fornara F, Fornari M, Ouwerkerk P B, An G, Colombo L, Kater M M. The D-lineage MADS-box gene OsMADS13 controls ovule identity in rice. Plant J, 2007, 52: 690–699
[9] Dreni L, Pilatone A, Yun D, Erreni S, Pajoro A, Caporali E, Zhang D, Kater M M. Functional analysis of all AGAMOUS subfamily members in rice reveals their roles in reproductive organ identity determination and meristem determinacy. Plant Cell, 2011, 23: 2850–2863
[10] Li H, Liang W, Yin C, Zhu L, Zhang D. Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy. Plant Physiol, 2011, 156: 263–274
[11] Cui R, Han J, Zhao S, Su K, Wu F, Du X, Xu Q, Chong K, Theissen G, Meng Z. Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa). Plant J, 2010, 61: 767–781
[12] Gao X, Liang W, Yin C, Ji S, Wang H, Su X, Guo C, Kong H, Xue H, Zhang D. The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development. Plant Physiol, 2010, 153: 728–740
[13] Lin X, Wu F, Du X, Shi X, Liu Y, Liu S, Hu Y, Theissen G, Meng Z. The pleiotropic SEPALLATA-like gene OsMADS34 reveals that the ‘empty glumes’ of rice (Oryza sativa) spikelets are in fact rudimentary lemmas. New Phytol, 2014, 202: 689–702
[14] Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano HY. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell, 2004, 16: 500–509
[15] Li H, Liang W, Hu Y, Zhu L, Yin C, Xu J, Dreni L, Kater M M, Zhang D. Rice MADS6 interacts with the floral homeotic genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in specifying floral organ identities and meristem fate. Plant Cell, 2011, 23: 2536–2552
[16] 王兆龙. 小麦小花发育的生理基础及调控研究. 南京农业大学博士学位论文, 江苏南京, 2000. pp 3–4
Wang Z L. Physiological Basis and Regulation of Floret Development in Wheat. PhD Dissertation of Nanjing Agricultural University, Nanjing, China, 2000. pp 3–4 (in Chinese with English abstract)
[17] 刘楠, 李海峰, 窦艳华, 韩德俊. 普通小麦及其近缘物种花序、小穗和小花的形态结构分析. 麦类作物学报, 2015, 35: 293–299
Liu N, Li H F, Dou Y H, Han D J. Morphology and structure analyses on inflorescence, spikelet and floret of bread wheat and its relatives. J Triticeae Crops, 2015, 35: 293–299 (in Chinese with English abstract)
[18] Paolacci AR, Tanzarella OA, Porceddu E, Varotto S, Ciaffi M. Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-like genes in wheat (Triticum aestivum L.). Mol Genet Genomics, 2007, 278: 689–708
[19] Ishikawa M, Ohmori Y, Tanaka W, Hirabayashi C, Murai K, Ogihara Y, Yamaguchi T, Hirano H Y. The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses. Genes Genet Syst, 2009, 84: 137–146
[20] Shitsukawa N, Tahira C, Kassai K, Hirabayashi C, Shimizu T, Takumi S, Mochida K, Kawaura K, Ogihara Y, Murai K. Genetic and epigenetic alteration among three homoeologous genes of a class E MADS-box gene in hexaploid wheat. Plant Cell, 2007, 19: 1723–1737
[21] Zhao T, Ni Z, Dai Y, Yao Y, Nie X, Sun Q. Characterization and expression of 42 MADS-box genes in wheat (Triticum aestivum L.). Mol Genet Genomics, 2006, 276: 334–350
[22] Zhao X Y, Cheng Z J, Zhang X S. Overexpression of TaMADS1, a SEPALLATA-like gene in wheat, causes early flowering and the abnormal development of floral organs in Arabidopsis. Planta, 2006, 223: 698–707
[23] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011, 28: 2731–2739
[24] 窦艳华, 韩萌萌, 孙其信, 李海峰. 二穗短柄草MADS-BOX基因AGL6和FUL1的可变拼接和表达模式分析. 农业生物技术学报, 2015, 23: 459–468
Dou Y H, Han M M, Sun Q X, Li H F. Alternative splicing and expression pattern analyses of two MADS-BOX genes AGL6 and FUL1 in Brachypodium distachyon. J Agric Biotechnol, 2015, 23: 459–468 (in Chinese with English abstract)
[25] Li H F, Liang W Q, Jia R D, Yin C S, Zong J, Kong H Z, Zhang D B. The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice. Cell Res, 2010, 20: 299–313
[26] Jack T, Brockman L L, Meyerowitz E M. The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS-box and is expressed in petals and stamens. Cell, 1992, 68: 683–697
[27] Ambrose BA, Lerner DR, Ciceri P, Padilla CM, Yanofsky MF, Schmidt RJ. Molecular and genetic analyses of the silky1 gene reveal conservation in floral organ specification between eudicots and monocots. Mol Cell, 2000, 5: 569–579
[28] Kobayashi K, Maekawa M, Miyao A, Hirochika H, Kyozuka J. PANICLE PHYTOMER2 (PAP2), encoding a SEPALLATA subfamily MADS-box protein, positively controls spikelet meristem identity in rice. Plant & Cell Physiol, 2010, 51: 47–57
[29] Yoshida A, Suzaki T, Tanaka W, Hirano HY. The homeotic gene long sterile lemma (G1) specifies sterile lemma identity in the rice spikelet. Proc Natl Acad Sci USA, 2009, 106: 20103–20108
[30] Hong L, Qian Q, Zhu K, Tang D, Huang Z, Gao L, Li M, Gu M, Cheng Z. ELE restrains empty glumes from developing into lemmas. J Genet Genomics, 2010, 37: 101–115

[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4] YUAN Da-Shuang, DENG Wan-Yu, WANG Zhen, PENG Qian, ZHANG Xiao-Li, YAO Meng-Nan, MIAO Wen-Jie, ZHU Dong-Ming, LI Jia-Na, LIANG Ying. Cloning and functional analysis of BnMAPK2 gene in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(4): 840-850.
[5] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[6] HUANG Cheng, LIANG Xiao-Mei, DAI Cheng, WEN Jing, YI Bin, TU Jin-Xing, SHEN Jin-Xiong, FU Ting-Dong, MA Chao-Zhi. Genome wide analysis of BnAPs gene family in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(3): 597-607.
[7] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[8] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[9] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[10] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[11] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[12] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[13] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[14] JIAN Hong-Ju, SHANG Li-Na, JIN Zhong-Hui, DING Yi, LI Yan, WANG Ji-Chun, HU Bai-Geng, Vadim Khassanov, LYU Dian-Qiu. Genome-wide identification and characterization of PIF genes and their response to high temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 86-98.
[15] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!