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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (01): 141-148.doi: 10.3724/SP.J.1006.2017.00141

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Regulation on Contents of Endogenous Hormones and Asr1 Gene Expression of Maize Seedling by Exogenous ABA under Low-temperature Stress

LI Xin-Yuan1,2,**,YANG Ye1,**,ZHANG Li-Fang1,ZUO Shi-Yu1,LI Li-Jie1,JIAO Jian1,LI Jing1,*   

  1. 1 College of Agronomy, Northeast Agricultural University, Harbin 150030, China; 2 Qiqhaer Branch of Heilongjiang Academy of Agricultural Science, Qiqihaer 161006, China
  • Received:2015-12-04 Revised:2016-09-18 Online:2017-01-12 Published:2016-09-27
  • Contact: 李晶,E-mail: jingli1027@163.com, Tel: 0451-55190304
  • Supported by:

    1 College of Agronomy, Northeast Agricultural University, Harbin 150030, China; 2 Qiqhaer Branch of Heilongjiang Academy of Agricultural Science, Qiqihaer 161006, China?

Abstract:

 

Abscisic acid (ABA) is an important signal factor under low-temperature stress. This study aimed to investigate the effect of exogenous ABA on growth regulation of maize seedlings under low-temperature stress. Maize variety “Jiulong 5” resistant to low temperature was weed with foliage spray of ABA (5, 15, 25, 35 mg L-1) at three-leaf period under varying degrees of low-temperature, to analyze relative electric conductivity, activities of autioxidant enzymes and contents variation of endogenous ABA and IAA of maize blades, as well as Asr1 gene expression level by Real-time PCR. The relative electric conductivity of maize blades showed an increasing tendency, meanwhile, activities of SOD and POD were enhanced by exogenous ABA treatments among which SOD activity was significantly higher in ABA treatments of 15 mg L-1 and 25 mg L-1 than in the control under low-temperature stress. Also the synthesis of endogenous ABA and IAA were increased significantly. The expression level of Asr1 gene significantly promoted in ABA treatments of 5, 15, and 25 mg L-1. ABA contant had a highly significant correlation with the relative expression of Asr1 gene and SOD activity and a significant correlation with POD activity. In conclusion the expression of Asr1 gene is mediated and regulated by ABA, the promotion of Asr1 gene expression also promotes the synthesis of endogenous ABA, and enhances antioxidant enzyme activities, resulting in improved maize resistance to low temperature. But the mediation and regulation of exogenous ABA have a concentration effect, showing promotion at low concentation and inhibition at high concentation.

Key words: Maize, Exogenous ABA, Asr1 gene, Low-temperature stress, Endogenous hormone

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