利用2个F2群体整合中国豌豆高密度SSR遗传连锁图谱

doi:10.3724/SP.J.1006.2020.04028

Abstract

Abstract:

Pea (Pisum sativum L.) is an important food legume crop grown widely throughout the world for humans or livestock consumption. Genetic linkage map constructed with SSR markers have played a vital role in marker-assisted breeding of many crops including pea. Public available and transferable SSR markers and genetic linkage map with sufficient SSR markers based on genetically distinct Chinese pea germplasm are limited despite a long study history on genetic linkage mapping in pea. In this study, in order to obtain more transferable SSR markers and high resolution genetic linkage maps for Chinese pea, 617 polymorphic SSR markers were firstly screened from 12,491 genome-wide SSR markers and some related literatures by our laboratory, and these SSR markers were used to construct an enhanced genetic linkage map for the G0003973 × G0005527 F₂ population. The enhanced genetic linkage map covered 5330.6 cM in total length containing 603 SSR markers with an average inter-marker distance of 8.8 cM, which was significantly improved both in marker number and in density compared with the previous map. 119 polymorphic SSR markers were screened based on the above results to develop a new map for a large W6-22600 × W6-15174 F₂ population including 118 SSR markers with a cumulative length of 1127.1 cM assembled into seven genetic linkage groups. Furthermore, data from the above two genetic maps were combined to build an integrated map of 6592.6 cM, comprising 668 SSR markers, 509 genomic SSRs, 134 EST-SSRs and 25 anchor markers distributed in seven genetic linkage groups. These SSR markers and genetic linkage maps will provide a valuable tool for the genetic study and marker-assisted breeding in pea.

Key words: pea (Pisum sativum L.), SSR, genetic linkage map, integrated map, marker-assisted breeding

LIU Rong, WANG Fang, FANG Li, YANG Tao, ZHANG Hong-Yan, HUANG Yu-Ning, WANG Dong, JI Yi-Shan, XU Dong-Xu, LI Guan, GUO Rui-Jun, ZONG Xu-Xiao. An integrated high-density SSR genetic linkage map from two F₂ population in Chinese pea[J].Acta Agronomica Sinica, 2020, 46(10): 1496-1506.

Figures/Tables 9

Table 1

Fig. 1

Table 2

Fig. 2

Table 3

Fig. 3

Supplementary table 1

Information of SSR markers used to generate the integrated genetic linkage map of pea"

序号 No.	标记名称 Marker name	标记类型 Marker type	正向引物序列 Forward primer sequence (5°-3°)	反向引物序列 Reverse primer sequence (5°-3°)	退火温度T_m (℃)	片段大小Band size (bp)	连锁群Linkage group
序号 No.	标记名称 Marker name	标记类型 Marker type	正向引物序列 Forward primer sequence (5°-3°)	反向引物序列 Reverse primer sequence (5°-3°)	退火温度T_m (℃)	片段大小Band size (bp)	整合图谱Integrated map	PSP1 G0003973× G0005527	PSP2 W6-22600×W6-15174)
1	27256	基因组SSR	CATTCCATTCCATACATCCATTT	TGAAGTTGAAGCAGCCATTG	60	153	I		I
2	26075	基因组SSR	CTATGGCACCATCTCTTGGAC	AACACAATGTATGTGGTGCAAAT	59	186	I		I
3	28142	基因组SSR	TGCAAGCATATTGCCTTTTC	TCAGTGGTTGCTAGCTGTTGA	59	176	I		I
4	26020	基因组SSR	CTCTCAAATTTGGGGTCCTC	TCATTGCGTCTCAACCTCAG	59	129	I		I
5	25334	基因组SSR	TGAAGATGTGACAAACAACAGAAA	TCCTTCTCTGTTCCCACCAC	60	140	I	I	I
6	23971	基因组SSR	GCGTGTTGATGCTGAAAGAA	TCAAAATTGGGGTGTGACAA	60	131	I		I
7	25922	基因组SSR	CCAAGGGAAAACCCCTTCTA	GTGTGAAAGCTTATTGTATATGTATCG	58	176	I		I
8	AD147	锚定标记	AGCCCAAGTTTCTTCTGAATCC	AAATTCGCAGAGCGTTTGTTAC	61	330	I	I-2	I
9	AA67	锚定标记	CCCATGTGAAATTCTCTTGAAGA	GCATTTCACTTGATGAAATTTCG	51	370	I		I
10	20229	基因组SSR	GCGAAGACTTCCGACCATTA	CACGGTCAAGGCCTACATTT	60	181	I	I	I
11	18339	基因组SSR	TGGTTGAACTGGAACGAGTG	TGAAATTGCAATGTAAGCATGA	59	137	I	I
12	e1154	EST-SSR	GCCATGCGACCATATTTACC	ATGGCCACAGAAACGAAAAC			I	I
13	e1111	EST-SSR	TTCTTCGTCGGAGGATGAGT	TTGAGAGGAGATTGGAGAAAAA			I	I
14	28832	基因组SSR	CTCACGCGTTGAAGATACCA	CACCGCCATTGTAGTACAGC			I	I
15	27227	基因组SSR	GCATTCCATGAATTGCATCT	TTGCTGATTTCTTACTTGTTGTCA	59	167	I	I
16	e734	EST-SSR	AGGCAGTGACTGAATCATCGT	AATGGCTTTGAGGCAGAGAG			I	I
17	18591	基因组SSR	AGGGCCGAATGCTAAGTGAT	TTTTGAACCCTGGAGGGAGT	61	155	I	I
18	21662	基因组SSR	GAACATTATCGGAATCAACAGC	CCACACAAAAATGAACAACACA	59	158	I	I
19	17158	基因组SSR	CTCCCGAGTCTTGGCTAATG	AGGCGCTCATAAACAGTTCC	59	175	I	I
20	e1171	EST-SSR	AGTCCCATCCCACGAAAAAT	CTCTTTCAAATCCCCCAACA			I	I
21	PEACPLHPPS	基因组SSR	GTGGCTGATCCTGTCAACAA	CAACAACCAAGAGCAAAGAAAA	52	120	I	I
22	e613	EST-SSR	CAATAATTTCACACACACCAAGAA	TCCAAAGAATCCTAAGAAACATGA			I	I
23	24312	基因组SSR	TGCCATATGCATTTCATGGT	AAGCCCCTTTTCATCTTCAA	59	210	I	I
24	e1013	EST-SSR	ACACCAACGATGACCCATTT	CCGATCCACAAACCGTTATT			I	I
25	29847	基因组SSR	TTGTTCCTACCGTGCTTTCA	TCTTATTGGCCTGCACATCTT			I	I
26	29428	基因组SSR	CCATCCACATCCTTCCAAGT	CACTCAACCCACGGAAAAGT			I	I
27	e700	EST-SSR	GGTTTCGGTTGATCATGGTA	GGAACTTTCTCTCGGGATCA			I	I
28	e1189	EST-SSR	TGGCAATTGCGATGATTAGA	TTCATCCGTTTCATGATGTTG			I	I
29	25805	基因组SSR	CCAACTTACTTTTGCTTATCTGGT	TGGGTCCATGACAAAGACAA	58	124	I	I
30	25096	基因组SSR	CAACATTGTTATCATCAAAACTCAA	GGCGACAATCGATCTCAAGT	58	192	I	I
31	28733	基因组SSR	TGGCCTAGGTTTTTGTGTCC	GCATCTCAAAAGGGCATTATTT	59	116	I	I
32	23546	基因组SSR	TCCACCTTGTTGCCCTAATC	TGAATGCTTCTCAGATACAAAATGA	60	155	I	I
33	22611	基因组SSR	TGCAAATGTGCAATGAATGA	GGCGGACATGAGAAGGAATA	60	187	I	I
34	27835	基因组SSR	CATCACTTGGGATTTCTTGAGAG	AGGGCAATGGTAATCAGCAC	60	138	I	I
35	27301	基因组SSR	TGTCGGAAATTAAGAGGTGGA	TGGAAAAGTAAGCGGTGAACA	60	119	I	I
36	e1226	EST-SSR	CACACCAGGTATCAATCTGTGAA	CGTTCCGCTTTTCACTCTCT			I	I
37	16881	基因组SSR	ATGGGCTTTAGGGGAAGAAA	AAAAGCAGCACATGGAGGAC	60	133	I	I
38	21678	基因组SSR	CCCTTCAGCAACAATCACTG	TGCCTCAGATTTGGAATGGT	59	151	I	I
39	20646	基因组SSR	TCTCACATGTTGTTATTTCTTTCTCA	TGATGTTCCCCAGATTTTCA	59	120	I	I
40	PsAS2	锚定标记	CTAATCACACGTTTAGGACCGG	CGAAATCCAAACCGAACCTAATCC	52	300	I	I
41	e888	EST-SSR	GGCTTCTCCATTTGTGGTTC	GCCAATGGAGGTTCTACAGC			I	I
42	18011	基因组SSR	GACCAACGACTTGGACATCA	GGTGAGTTCCTAAGATGAATCAGA	59	197	I	I
43	e811	EST-SSR	TTTTGTGGGTCTCTCTTCACC	CACCACACATGCAACACTCA			I	I
44	27246	基因组SSR	CCAGGTTAAAACGATGATTTTTG	ACTTTTCCCCTTGGTTGGAC	60	180	I	I
45	3023	基因组SSR	GGTGCAAAATTTGAGGTGCT	CACACACGACTACACACACTACG	59	153	I	I
46	27167	基因组SSR	GGCACAACTACAACCCACAA	GGTTCAGGAATGGGTTCAGA	59	194	I	I
47	e799	EST-SSR	TGCAGGCTTTAGAAGTTGTTCA	CTCAGCAGCCACAATTACACA			I	I
48	5897	基因组SSR	GGCAATAACTTAAGAGTACTAAGGAAA	AGGGTGTCGTCGTGTGTGTA	57	282	I	I
49	27361	基因组SSR	CTGAAACGGTTTGCATTGTG	TCCAACCACTTCTTAACAACCT	58	113	I	I
50	22209	基因组SSR	AATCCACAACCCCGTCAATA	CAAAAGAGACCTTCTTCCTCTCA	59	164	I	I
51	22259	基因组SSR	CATCATGGCTCAATCTCAACA	TTCCCAAATTCCTTCGTTCA	60	110	I	I
52	22406	基因组SSR	GGAAAGAGTTATGGCAATGGAC	TGGTGGTGGAGCTAAGTGTG	60	174	I	I
53	24555	基因组SSR	CGCTTATGTAGCCCCTTTTG	GGCCAAAGGAGATTTGTGTC	60	123	I	I
54	24499	基因组SSR	AAAACAAACAAAACCGCAATG	TAGCCATCACCAAAGCAACA	60	169	I	I
55	e624	EST-SSR	CCTTAGCAAGTTTGTCTTTGAGTG	TGCAATGACATGATGGAAGAA			I	I
56	29029	基因组SSR	TAGGAGAGCGAGGAGCAAAG	CCACCAAAAGCAAGAATGTG			I	I
57	e884	EST-SSR	TTCTTTCCGCCATGAGATTC	GAGAGCAAGGGTTTGGAACA			I	I
58	28438	基因组SSR	GGAATGACGAAGTAACCACCT	GATGCAAGTGCAACCTTTGA	58	185	I	I
59	17628	基因组SSR	GGTTTTGTTTGCCGTTGATT	CCACCCCCAAACTTCCTTAT	60	153	I	I
60	17605	基因组SSR	CGCCCTTCATCATCATCTTC	AGAGTCGGTCCCTCCAACAT	61	150	I	I
61	1356	基因组SSR	CACGTGCACATACACACTCTT	TGTGTCAGAGCATGTGTTCG	57	106	I	I
62	171	基因组SSR	CAAACACACACGCACACAAA	CGTGTGAGCGTGCATAAGT	58	70	I	I
63	28654	基因组SSR	AGCGACGTGAATATCACAATG	GTTATCGCGGCGTGTAAATC	59	134	I	I
64	e658	EST-SSR	TGGTTTCTCTGCCAAAACAG	TGATGAGTGGTGACGCAAAT			I	I
65	e562	EST-SSR	CAAGATGCTTCTGATTCAGTGTC	AGGATTTGAGCTTGGGAGGT			I	I
66	17989	基因组SSR	CAGAGCCGGAGTTCTGGATA	TTTGGTTGACATTAGCACATGA	59	195	I	I
67	26333	基因组SSR	AAACACACGACATGTTTCCTTTT	TCACTGCAATTCGTCGATGT	60	116	I	I
68	D21	锚定标记	TATTCTCCTCCAAAATTTCCTT	GTCAAAATTAGCCAAATTCCTC	51	200	I	I
69	18928	基因组SSR	TGAATGTGGAAAGGAGGAATG	AGGGTCACCACTTTGGAGAG	59	178	I	I
70	18529	基因组SSR	GAATGTGCGTCCAACATCCT	AGATTTTGATGCGGAAGAGC	59	151	I	I
71	1863	基因组SSR	GCACACGAATACAGTCACGC	GTGTGTTGACGTGCGAGTTT	60	118	I	I
72	e997	EST-SSR	GCCTGGAGTGTTGAAGAGGA	CCATCACAATTTCCCACACA			I	I
73	1752	基因组SSR	GCACGCACACGAATACAGTC	GACGTCGTGAGTTTGCATGT	60	115	I	I
74	AF016458	锚定标记	CACTCATAACATCAACTATCTTTC	CGAATCTTGGCCATGAGAGTTGC	54		I	I
75	23261	基因组SSR	CTGCTTTTGGGGTTTGGTTA	GCAATGCAACTCCTCAACAA	60	156	I	I	I
76	22699	基因组SSR	CAACATGCCATTCTGGCTAA	GCCGAAACCCCATGTAGAC	60	157	I	I
77	24895	基因组SSR	AAGAAAGTTGCGTTGGATGTG	GTTTTGTACCGCCCAACACT	60	148	I	I
78	24731	基因组SSR	AGAAAATGGCCCACGAATTA	TGCATTGCATTGTGTTTGTG	59	204	I	I
79	25106	基因组SSR	AAGGCCAAACAGAAAGGAGA	CAATGTCCAAGAAAGATCCAGTT	59	178	I	I
80	4083	基因组SSR	TGCAAACTCACACGTCAACA	GTGCGTGTGCGAAGTACG	60	191	I	I
81	1753	基因组SSR	GCACGCACACGAATACAGTC	TCGTAGTTTGCATTGTGCGT	60	115	I	I
82	25341	基因组SSR	AATGCTTCTTCCACGGTCAC	TTCGCTCGAGTTCGATTCTT	60	184	I	I
83	25454	基因组SSR	TTCCAAGCAAGCGTTGAAGT	TCAAGAGAGACTTTTCAAGAGGTT	58	204	II	II
84	29797	基因组SSR	TGTGATCAGGTGCTCCCATA	GCGACAAATTATGGCTATGC			II	II
85	26436	基因组SSR	TTGCCTTGCCAACTTTTAGG	CTTGCTTCTGCGCCATAAAT	60	195	II	II
86	e581	EST-SSR	CCTTGATGCCACAAATGAGA	TTGCCACTTTCTCAAAAACTCA			II	II
87	S217	基因组SSR	CACTCAACTCACCGACCTCA	GACGGATGGAAATTGGTGTC	52	1035	II	II
88	e956	EST-SSR	CGAGCGTGAGACTGTGATGT	TCCACCGGTTCAACTTCAAT			II	II
89	e344	EST-SSR	ATGCAACCGGCGCAGTAT	CCACCTTTTCCTCGCTTTTT	61	159	II	II
90	e967	EST-SSR	TGACACTTTCGTGTACTGTGTTTTT	TTCCAAAAGCCTCTCTTTCATC			II	II
91	20922	基因组SSR	AAAAGGAGAACACATTTTATAATAGCA	TGCTCTTAAAGGCGACAATG	58	146	II	II
92	29141	基因组SSR	TTCTTTCTGCTAGGAGCCACT	CAAAGCCATCACCCTACACA			II	II
93	24959	基因组SSR	ATCCTCACCGGTTTGATGAC	TGGAGAGTGATAGAGAAAAATTGTG	59	115	II	II
94	25059	基因组SSR	ATGGATTGCGGATAGCTCAA	CAGCAGTTGTTCGCAGGTAA	60	189	II	II
95	24301	基因组SSR	TTGTGTTTTCCGGAGAGGTC	TCCCTCCCAACCTTGAATTT	60	142	II	II	II
96	AA332	锚定标记	TGAAAATAAAGGCATGCAAATA	TGATTAGTCAACTTGTTGTGGA	51	255	II		II
97	29220	基因组SSR	GGGGCAGATTTGTGGTATTG	TTCTTCTTCCTCACGTCTTTCTTT			II		II
98	AD83	锚定标记	CACATGAGCGTGTGTATGGTAA	GGGATAAGAAGAGGGAGCAAAT	61	270	II		II
99	18272	基因组SSR	CCCCAACATTTCTCTAGGTAACA	TTCTTCGCAGCTCGGTAAGT	59	131	II	II	II
100	S42	基因组SSR	AGTTTCGGGTTCCTTGGAGT	GTTGGCGTAGAACGATCCAT	53	211	II	II
101	e198	EST-SSR	ACCATCACCACCAACAACAC	CTGCATCTGGAGAGGGAGAG	59	188	II	II
102	e14	EST-SSR	TCCGCAATGTTCTCTCGAAT	GGAGGTCTCCGCATTATCAA	60	188	II	II
103	21776	基因组SSR	AACGGATATGCATGGAGAGG	AAAACACGACCATCCTTTGTG	60	172	II	II
104	21939	基因组SSR	GGTCCTCAAGCACCACCTAA	TGGGCGTCACTACTTAACTTTT	58	114	II	II
105	28257	基因组SSR	AAGGGCTGACGGTCTAACTG	GAACTGACGGACGCTAGAGG	59	161	II	II	II
106	16237	基因组SSR	GCAAACGAAGCAGGCTTATC	TTGGCTGATCCTGAAACTGA	59	152	II	II
107	18260	基因组SSR	AACCTTGAAATGGAGGTACATGA	GACCATGATCGGATGTTGTG	60	129	II	II
108	21641	基因组SSR	CGATTTACCGTCCTTCATCA	ACGGTCTCCCATGTGTTTGT	59	166	II	II
109	3015	基因组SSR	ACACATGCACACCCCCTTC	TGCGTGTGTACGTGTGTACG	60	153	II	II
110	D23	锚定标记	ATGGTTGTCCCAGGATAGATAA	GAAAACATTGGAGAGTGGAGTA	51	170	II	II
111	e1031	EST-SSR	CAACACAAGAACTTTGCACCT	TTGATCCACCTGCATCATTG			II	II
112	e1215	EST-SSR	GAGACAGAAGACGGCGAAAG	TGCCAAGAGTCAGGAGATTG			II	II
113	16433	基因组SSR	CACCGCAAACATAGCAAAAA	TCTCATAGCTGCGAGGTTCA	60	127	II	II
114	e617	EST-SSR	GCCAAACGGCTTTAAAACTTC	TCGCTGTTGGAAAGAGAAGAA			II	II
115	20723	基因组SSR	CTCACTTCACGTGCGCTATC	GCAGGAGCAGCTTGATTTTC	60	152	II	II
116	16437	基因组SSR	TTGTTTTTGTTGTTCTTGTTGTTG	TTTTCGGGTTTTGCTTATGG	59	128	II	II
117	17531	基因组SSR	TGCAGGGGTGTGTGTTACAT	TGAACATGGTGAAATGGATTG	59	140	II	II
118	18237	基因组SSR	GGGATATGAGAAGGCGATACC	TGGTTGTAGGATGTGGGATTT	59	127	II	II
119	17754	基因组SSR	AGCAACGGGCAACCTTATAG	CCTTTTGTTTGGAAGCTCAA	58	169	II	II	II
120	e850	EST-SSR	TTTCTTCTCCCAAACTACCTCAT	ATGCATGAACCAACCCATCT			II	II
121	17713	基因组SSR	AAAAAGGGGAAAGCAGGAGA	TTGACTGTGAGGCTGGTTTG	60	164	II	II
122	21945	基因组SSR	TTCACGCTCATCGCTAAGAC	TTCGAATCCTCCCTTCTTGA	59	113	II	II
123	e478	EST-SSR	GCCACCAACCAATTCAACTT	TGGGTATTGGGAATGGAAAA			II	II
124	e36	EST-SSR	GCAGGGTGGGTATATCTGTGA	GTGGTCCAATTCCTTTTTGC	59	219	II	II
125	e26	EST-SSR	TTTTGTCCCCGCGTTTAATA	CATTCATGCCACAAAAATGG	60	155	II	II
126	C24	基因组SSR	GCTACTGGAGGAGGCTTTCA	GCCTTCTACACAACGGCTTC	52	162	II	II
127	28383	基因组SSR	TCGATTGTTATTGTGTTTCCTCTC	TGAGATCAAGTGGGGGAAAA	60	150	II	II
128	e770	EST-SSR	GGTTTGAAAGGACCCCTAGC	GTTACCGATGGCCATGAATC			II	II
129	143	基因组SSR	ACATGCACACGTACACGCA	AGTTGGCGTGCAGTAGAGGT	60	69	II	II	II
130	16534	基因组SSR	TTGCAAATATACCAATTCCAAAA	ATTGGAGCCTGGTGAAGACC	58	139	II		II
131	28055	基因组SSR	GCCAGCAATTTTAGCATTACG	TTAGCTCAGCCCGGTAAAAA	60	163	II		II
132	25792	基因组SSR	GACGGAAACGAAATCGAAGA	TCAAAATTCACGCACACGAT	60	110	II	II	II
133	30024	基因组SSR	AGAGTGCCATCCCTTCAATG	GAACGTTTGGTTGGAGGAGA			II		II
134	25769	基因组SSR	GCAGCAAGATGGTTGGTAGTT	GACGTCGTAGTCGCCATCTC	59	144	II	II	II
135	e929	EST-SSR	TGAGGAGGGAGATGGAGAAA	GAAGGCAAACCTACCAACCTC			II	II
136	30218	基因组SSR	GGACGTGTCCCACTCCTATG	GGAAGGATAAAAACGTTGCAATA			II	II
137	30416	基因组SSR	GACACATGGAGCCACAAAAA	GAATGGAGGGGAGAGATGAA			II	II
138	e577	EST-SSR	TGTCATTTCTTTTTAGTTCCTTTCAA	CCTTCGCTTGATTCTTCACC			II	II
139	16570	基因组SSR	CAAACACCAACCACCACAGT	AAGGGGAGACGAAGTGGAGT	59	143	II	II
140	27099	基因组SSR	GGTACACCCACCGATACACC	TCTAGACGCGGAGAGGGTAA	60	140	II	II
141	27112	基因组SSR	GCAACAAGATTTTGACGTTTTT	GACGCTACCAACCGCTTTAG	58	129	II	II
142	28687	基因组SSR	CACGGAAGGCCCTACTTACA	GTGGCGAGTAGAGCGTAAGG	60	189	II	II
143	28704	基因组SSR	TTCTGCAGTCAGCTTCAACTTC	TAGTCACGGAAGCGATTCAA	59	147	II	II
144	22690	基因组SSR	GGTTCATCTGCACCCAAGTT	GGCAACTCTCTCACACACACA	60	138	II	II
145	24812	基因组SSR	GACCAAACCACCTCACAGATG	TGGCTCCTTTCTCATTTCTAACA	60	140	II	II
146	23154	基因组SSR	AAGACGAGGTGGCATGGTAG	GAGAATGCATGCTTCAATCAA	59	183	II	II
147	28785	基因组SSR	GATCCACCCAATTCCCTTTT	TGTATTGCAGCCGCTTTATG	60	208	II	II
148	19680	基因组SSR	GCCAACCCAACAATCTCAAC	CATTGGAACCAGATCGAACC	60	148	II	II
149	22052	基因组SSR	GTTACCGATGGCCATGAATC	AGCGAGTGAAGAGGGAAGTG	60	147	II	II
150	1078	基因组SSR	CACACGCACAGACACACGTA	GTGTGCGTATGCGTTACTGC	60	99	II	II
151	29138	基因组SSR	GCAGATTGAAACCAAAACGAC	TCGCAACCTGCACTTTCTTA			III	III
152	e763	EST-SSR	ATGCTTTGATGGGCTCAACT	TCCCAAACATGCTAGCAAAA			III	III
153	19532	基因组SSR	CCATGTTTGAATTCGGAGGA	GCGCGATGATTCAAGGTTTA	61	165	III	III
154	19656	基因组SSR	CCAACGTTGTTGTTTTAGTGG	CCAGAGTCGTGGAGCCCTAT	58	169	III	III
155	27876	基因组SSR	TGTTGTTGCCCATCAATCAT	AATCACACGAGGGATTGGAC	60	149	III	III
156	18323	基因组SSR	CAGACAATGGCAATTATTTGGTAA	CTGCTGTTGCTTCGATTTCA	60	136	III	III
157	21881	基因组SSR	CCATTCCCAACAATTTCCAC	GTGAGGTCCGGTTCTACAGG	60	119	III	III
158	30088	基因组SSR	TACTGGATCCGGATGAGGAC	TCGCATCAAAGCAAAAACCT			III	III
159	e605	EST-SSR	AGCACTTGCTACGGCAATTT	AAACCTAGAAATAACGATGCAAAA			III	III
160	24265	基因组SSR	GTTTGCGGCCAAACAATATC	TTTGCATGAGTGCACCTCTC	60	181	III	III
161	29627	基因组SSR	AGAAGACAACGACCGAGTGG	AACCGCATAACCGCAATTTA			III	III
162	27019	基因组SSR	GCAGTTTCCACACTTTAAGTCCA	TGGGTGTGTTAACAGGGTGA	60	155	III	III
163	e1208	EST-SSR	AACCATTGCGCGTCTTTTAC	AGACCACCGCCATAACATTC			III	III
164	28130	基因组SSR	CGAATTTGGTTGCGACGAC	TCGCGCCTCTTTAGGAATAA	60	208	III	III
165	27378	基因组SSR	GCCAATTATTCCCTCCAGGT	TTCGAAGGTTCTCCATCACC	60	147	III	III
166	28180	基因组SSR	TCGTTCATTTAACTTCGTGAGGTA	AGAGAGTTCTCCGCCAAACA	60	201	III	III
167	24903	基因组SSR	TGGTGTCAACTTTTTGATGTTCA	GACAAGTTGCTTTTGCTCCAC	60	147	III	III
168	26850	基因组SSR	TCACAGACAGTACACAAAGTTTTCTT	GCGAGGGAGAACAGAAACAG	59	160	III	III	III
169	24521	基因组SSR	AGGGAACCCCCAATTGACTA	CCAGAAACTGGGGTTGTGTT	60	208	III		III
170	23311	基因组SSR	TTTCAGAATGGTGCAGGGTAT	AGGATCTCAGTATACATGCGTAAA	57	168	III		III
171	24540	基因组SSR	CTCCCTCATGAGTCGTGACC	ATCAAAGGGGGAAGGTGAAG	60	143	III		III
172	26575	基因组SSR	GAAAATAAACAGTTGGCAACAAAA	CCACTCCAAACCCTTCAGGT	59	205	III		III
173	21726	基因组SSR	GGTGATGGAGAAAAGGGTGA	TGCATGCAGTCAAATCAAAA	59	157	III	III	III
174	23518	基因组SSR	CAAGGACGACGACAACAACA	GTGCCGACGTTCAAGAAAAT	60	202	III	III
175	e632	EST-SSR	AAACCTCTCTACAGCACCAACAC	GGGAGAGATTGTTTGAAGTATAGAA			III	III
176	28406	基因组SSR	CCGATTGTGCAGCAAGAGTA	ACGATGCACATGCAGAATTT	59	129	III	III
177	27038	基因组SSR	CGTCTACCTCCGACGATAGC	TTCGCCAGATATATACAATAAAAAGA	58	187	III	III
178	25151	基因组SSR	GCCTTCGAGGCATCCTAAT	TGGAACCATAAGATTGGTGAA	58	157	III	III	III
179	27270	基因组SSR	GGACCATTACCCTCCCATTT	TTCCTTCCGTTTTGCAGTCT	60	194	III	III
180	28194	基因组SSR	TGGGGTCTTAAAGTTGTGACTTC	CCGAAGGTGGGATGAAACTA	60	148	III	III
181	28645	基因组SSR	CTGAAATCGGAGTGGTCACA	GGTGAAGCCCTTAGCTACCA	59	188	III	III
182	17193	基因组SSR	CACAGCCATACCCAAGTTACAA	GGTTGCGAGGGATGAGAATA	60	178	III	III
183	e814	EST-SSR	GGTTGGTCCAATCCAACATC	AGAACGAACACACACGAAACA			III	III
184	e494	EST-SSR	GACCCGTCTGGACTGGTAAA	TTGAAAGATGCGGAGTGATG			III	III
185	29436	基因组SSR	TCTAGCAGCATTGGGGAAAC	TAATGAGGGGAAGGGGATTT			III	III
186	27710	基因组SSR	AACCACAGAAAACTGCCAAGT	TGAGAACCAAAAGCAGGTCA	59	110	III	III
187	29074	基因组SSR	TCGTCGAATGGTTGAAGAGA	TTCGCAAGTGAAGGAAAAAGA			III	III
188	29307	基因组SSR	GCGATTCCAGATGTCAGGTT	CGTCTCCCTACCAGCAAAAA			III	III
189	24534	基因组SSR	GTGGTGTGAAAGGGGTTTTG	CTTGCATTGGATTCCCTTTG	60	206	III	III
190	22543	基因组SSR	TTTCACGTACGTTCCCAACTC	CCAGATCAACCACCTAACTTCA	59	160	III	III
191	23262	基因组SSR	GGTGACGGAGGTGATAGAGG	TAGCAAATGCAAACCCAACA	60	183	III	III	III
192	24701	基因组SSR	TATGCTGGAGTGTGGAGTGG	TCAATCAATTCAACGGTACAGA	58	111	III	III
193	24784	基因组SSR	TTTAGACGGCCTTCGTTAGTG	CTGAGCCTAAAGGGCTGAAA	59	115	III	III
194	27254	基因组SSR	GAAGGCCTCTAACGGTGAAA	AATCAAACAGAGGCCACCAG	59	122	III	III
195	22754	基因组SSR	GGAACGACAACACGAACCTC	GACACGTTATGCGCACACTC	60	161	III	III	III
196	26131	基因组SSR	GGAAACGGTGGAAGATGAAA	TTGGCAAAAGGGATGAGAAG	60	175	III		III
197	24805	基因组SSR	TGCAGCAGATCAACCAAAAC	TTTTGAACTAAGGTGGTCTCAATC	59	144	III		III
198	18135	基因组SSR	CTTCAACCAACTGCGAGTGA	TCATTTGAGTTTTGCCATGTTC	60	120	III	III	III
199	PSAJ3318	锚定标记	CAGTGGTGACAGCAGGGCCAAG	CCTACATGGTGTACGTAGACAC	58		III		III
200	29634	基因组SSR	CAATTAACAAACGCAGCCTTA	TTAGCCCGTGGATTTTCAAC			III		III
201	26866	基因组SSR	CGATACATTAAGGGCGGAAC	TGACTCATTCGCATTTGGAGT	59	186	III		III
202	23757	基因组SSR	TGAAAGAGGGGAATTGAGAGA	TCAGGTTACAAGCCCGAGAT	59	187	III		III
203	AA355	锚定标记	AGAAAAATTCTAGCATGATACTG	GGAAATATAACCTCAATAACACA	51	180	III	III	III
204	23899	基因组SSR	CCCCATCCTTGTGAACAAAT	ACGGTGTTTTGGTGGTGAAT	60	151	III		III
205	AD270	锚定标记	CTCATCTGATGCGTTGGATTAG	AGGTTGGATTTGTTGTTTGTTG	51	290	III		III
206	20075	基因组SSR	GCCAGTCCCTTGAGTTAGGA	TGTTTCACGTGTTCCCCATA	59	122	III	III	III
207	e696	EST-SSR	CGCTATCACTCTCTCTTTCTCTTTT	ATCGGAGGACGAGGTCTTTT			III	III
208	e1021	EST-SSR	AAAAAGCTGAAACATTCAGACC	GGTCCATTCATTCTGCAGTG			III	III
209	e673	EST-SSR	GGAAGACGGAGTGGTGGTAA	GTCGTCGTGGTGCTTCTCTC			III	III
210	16524	基因组SSR	CCAGAGGATGTGAACCAGGTA	TTCAACCAAGCTGAACCCTTA	60	138	III	III
211	4826	基因组SSR	AACATGCGTCTGTCGTGTCT	TAGTGGGTGTGCGTGTGAGT	59	220	III	III
212	27093	基因组SSR	TCGTCATTCTCCCTCCAATC	TATGATGTCCACGCGTTTTT	59	128	III	III
213	19688	基因组SSR	GAATCGGGTCGCTGAGATAG	ACCTCCACCGTACCATTCAA	60	133	III	III
214	e998	EST-SSR	GGCAGACTGGTCTCAACTCC	ATCATCGTTGGTGGAATCGT			III	III
215	2875	基因组SSR	TATTAGCACCCCTCACGTCC	TTTCCCCTTCCTTCCAATCT	60	148	III	III
216	30442	基因组SSR	CCCACTCCATCAGTCTCTCC	AGAGGACCGGTGATGTGTTC			III	III
217	e446	EST-SSR	AACCAGAGATGAGTGGAAAAATG	GCCAACACCAGAGTTTGAATC			III	III
218	27160	基因组SSR	CTGCAGTTGCGTGTAGAGGA	TTGAATGATGATATAAATGCAATGAC	59	135	III	III
219	e472	EST-SSR	TCCATCACCAGGCATAGGTC	GCCGGTAGTGAGAAGGATTG			III	III
220	864	基因组SSR	CACATACATGCAATCAAGCG	GTGTGTTACGTGCGTGTGTG	59	92	III	III
221	24861	基因组SSR	TTCACAACCCCTCTCACTACA	TGGAGGGATGGTTTACAATGA	58	183	III	III
222	30382	基因组SSR	TTGGTGAGGCCTTGATTTTT	GCCAGTGGGGATTAGAGACC			III	III
223	17773	基因组SSR	TTCCACACGAGGCTATTTTC	TGCAAAAGCGACATCTTGAC	58	170	III	III
224	18363	基因组SSR	CATGCATGGAGTTGGAAGAG	GTCCCAAAATGCAGCCAATA	59	139	III	III
225	29125	基因组SSR	TCAGAGGTGTCATCGGTCTG	TTTCAAATAAGTTTTGAACAAAGTGT			III	III
226	e999	EST-SSR	GTTTAGGAGCCTTGGGGTGT	TCCAAACTCCGGCTTCTCTA			III	III
227	3647	基因组SSR	GGGGTCTTACAACACACGCT	AGGCAGAGGTGTGAGCATCT	60	176	III	III
228	6144	基因组SSR	CAGAAAAGGAAGCAAGGTGC	GTCGCCCTCGATTCTCATAC	60	297	III	III
229	4535	基因组SSR	CAGAAAAGGAAGCAAGGTGC	TGTGTGTACGTGTTCACCCTT	59	206	III	III
230	3374	基因组SSR	GGGGTCTTACAACACACGCT	TGAGCTAATCTCTCCGGGAA	60	165	III	III
231	2169	基因组SSR	GTTGTGTGTGTGCGTGTTCA	GGGGTCTTACAACACACGCT	60	126	III	III
232	1935	基因组SSR	GGGGTCTTACAACACACGCT	TGTGTGCGTGTTCACCTTTT	60	120	III	III
233	2303	基因组SSR	GGGGTCTTACAACACACGCT	TGTTGTGTGTGTGCGTGTTC	60	130	III	III
234	4653	基因组SSR	CAGAAAAGGAAGCAAGGTGC	TGTGTGCGTGTTCTACCCTT	59	211	III	III
235	5347	基因组SSR	CAGAAAAGGAAGCAAGGTGC	AGCATCTCTCCGGTAACCCT	60	248	III	III
236	2008	基因组SSR	GGGGTCTTACAACACACGCT	TGTGTGTGCGTGTTCTACCTT	59	122	III	III
237	5412	基因组SSR	CAGAAAAGGAAGCAAGGTGC	GTGAGCAATCTCTCCGGGTA	60	252	III	III
238	2051	基因组SSR	GGGGTCTTACAACACACGCT	TGTGTGTGTCGTGTTCTACCTTT	60	123	III	III
239	5400	基因组SSR	CAGAAAAGGAAGCAAGGTGC	GTGAGCAATCTCTCCGGAAC	60	251	III	III
240	5348	基因组SSR	CAGAAAAGGAAGCAAGGTGC	AGCAATCTCTCCGGTACCCT	60	248	III	III
241	4670	基因组SSR	CAGAAAAGGAAGCAAGGTGC	TGTGTGTGTCGTGTTCTACCC	60	212	III	III
242	5540	基因组SSR	CAGAAAAGGAAGCAAGGTGC	AGGCAGAGGTTGTGAGCAAT	60	260	III	III
243	21506	基因组SSR	ATAGGGGGAGCAGGACCTAA	TTTGACTTGTGGAAAGGAAGTT	58	151	III	III
244	25416	基因组SSR	CGCCCAATTGGATTATGATT	TGCTCAATGCACACTTACTAGC	58	145	III	III
245	e1197	EST-SSR	TCCAACCGTTAAACACTCTTCA	GCATGAAGGGCTCTGAGTTC			III	III
246	PSBLOX13.2	锚定标记	CTGCTATGCTATGTTTCACATC	CTTTGCTTGCAACTTAGTAACAG	56		III	III
247	18562	基因组SSR	TTCTTCTGCTGCTGCTCAAA	AAAACAAAAACCACAACCAAAAA	60	154	III	III
248	21610	基因组SSR	CGATTGATGCCGTGTCTAAG	TTTCAAGTTTCTTCTAGATTTTGTCA	58	114	III	III
249	27991	基因组SSR	AATACAGCTGGACCCCACAC	GCAGGCCATTTCATTTCATT	60	121	III	III
250	22724	基因组SSR	CCCAAGAAGAAGGATGGTGA	GAGCATTCTGGTGCTGTTGA	60	152	III	III
251	26028	基因组SSR	CGGCGAGATTTATTGACGAT	CAACGTGGCAAGCAAGTAGA	60	186	III	III
252	4397	基因组SSR	TAATCTCTCCGGGAACCCTT	TGATATGATGCCATGAGGGA	60	201	III	III
253	20084	基因组SSR	CTCCCTCCCGAATGTAATCA	CGGGACGATCAACTTTGTCT	60	111	IV	IV
254	16758	基因组SSR	CCCTTCAACAAAGCCTAACG	AGGGTGCGAAGGAGGTTAGT	60	115	IV	IV
255	21712	基因组SSR	CGGCGGGTTTGATAGAGTTA	CTTCACCCTTGCAACAAACA	60	166	IV	IV
256	21774	基因组SSR	GCAAGTTCCCAATCGTCCTA	TCAAAAGCAAGGTCCCATTC	60	123	IV	IV
257	e671	EST-SSR	TTGCCTCATTTATCATTCTCTTATG	CAAAAGGTTATCTAGCTACGACTTGA			IV	IV
258	29964	基因组SSR	CAATTCATGACGAAATTGACAAA	CATGGAGATGGAGAGTTCAAAA			IV	IV
259	e996	EST-SSR	GCCGGTAAACGATCCATCTA	TGCAGCCACACTCCTTTACA			IV	IV
260	19632	基因组SSR	AATGTAATTAACCCACGAAGTTG	TGCCAAAGCTCTCTCATCCT	57	201	IV	IV
261	21547	基因组SSR	TGAAAGCCTCAAAGCAACAA	CCATGGCATGTGCTAGTGTAG	59	156	IV	IV
262	21250	基因组SSR	GTGCAATTTTCACACAGTGG	ACGAAGGTTGGAGCATGATT	58	144	IV	IV
263	22067	基因组SSR	TATGCTCAGAGGGGCATAGG	TTACGACGATGAGCGACTTG	60	158	IV	IV
264	18533	基因组SSR	TCCAAAATGCGTGTCATCAT	TGACCGACACATTCATCTTCA	60	151	IV	IV
265	26592	基因组SSR	TCCGATCCTGGTAAAGTGGT	TCCAAAATGCGTGTCATCAT	59	174	IV	IV
266	26369	基因组SSR	GCTGAAACGTGGGAAACATT	TGGTTAGTGTTTGAAGGGTCTG	59	206	IV	IV
267	25046	基因组SSR	TCCTTTGTCAGTGGGAATTTTT	AGGATCATGGTTGTCGAGTTG	60	173	IV	IV
268	24606	基因组SSR	CCGAACAAGATGAACCACCT	AACAACATCGTGTGTGTTTGTCT	59	208	IV	IV
269	23750	基因组SSR	CTCGTTGTACAATCCAGATGAA	CACCGTCCACCTTCTCATTT	58	209	IV	IV
270	22599	基因组SSR	GGGTGTGAGGCAGTTGAAAT	AAATACCGAACCGAACCACTT	60	141	IV	IV
271	24038	基因组SSR	GCCCCCACTTTTTCAACA	CTCCTGACACAAGGCCCTAC	59	145	IV	IV
272	18438	基因组SSR	GATTGAGCCGTGCCAATATC	GATCCCACCCTAGAGGAAAAA	59	145	IV	IV
273	22352	基因组SSR	CCAACATCTTCCTCATCACCT	TGAGAGTCGCAGTCGGATAA	59	129	IV	IV
274	16512	基因组SSR	TAAGCCCGACGCTTCTATTC	GTGCCTCAGTTTCCGTTTGT	59	136	IV	IV
275	AA430942	锚定标记	CTGGAATTCTTGCGGTTTAAC	CGTTTTGGTTACGATCGAGCTA	54		IV	IV
276	17219	基因组SSR	TCATGTGCATGTGATGAAGAAA	GGTGTACCCATGTGCCATTT	60	181	IV	IV
277	28178	基因组SSR	TCAACCCATACTCTTGGAATCA	CCGGAGATTCCACAAATAACA	59	139	IV	IV
278	28085	基因组SSR	TGCTTGCAACGTTTCTTTCTT	ACCCCTCCCTGAAAGGAGTA	60	142	IV	IV
279	e538	EST-SSR	CTTCCCTTTCTTCCCTTTCA	GTTCGGAAGGATCGATTTGA			IV	IV
280	28374	基因组SSR	TCCACGGTCTTGCTATGTGT	CTGGTTGCACATCAGGGTAG	59	187	IV	IV
281	e578	EST-SSR	AGCAGCTCATATTCTCTGTCCA	GCAGAAGCAGGATCTAGGGTAG			IV	IV
282	e656	EST-SSR	AGCAGCTCATATTCTCTGTCCA	AGCAGAAGCAGAAGCAGGAT			IV	IV
283	e650	EST-SSR	GCAGCTCATATTCTCTGTCCA	AGCAGAAGCAGAAGCAGGAT			IV	IV
284	29955	基因组SSR	TCAAGTGCATTGGGAGAGACT	AAAAACCGACCCATAATCAATTT			IV	IV
285	e1073	EST-SSR	CTTGTTTCGCTCGGTACCTC	CTATTGCAGGCAGTCCTGGT			IV	IV
286	24844	基因组SSR	TTTCGTTTTCCCATTCTGGT	CCCCCTTACACACGAATCTG	59	121	IV	IV
287	26018	基因组SSR	TTGAGCTGCTCGCTGTAAGA	CACCAAACTGTTTCTTCACACA	59	146	IV	IV
288	26179	基因组SSR	GGAAGGTGAAATTCCGTTGA	AGCAAGTTGGTTCGGAAAAA	60	166	IV	IV
289	24485	基因组SSR	GTGTCAAAGCTCGCCCTAAT	ATTGGTACACTTGCCGAGAA	58	127	IV	IV
290	e1130	EST-SSR	GCTGCTACCGTGGATTGTCT	TTGAAGAGGTGTGGTGTGGA			IV	IV
291	e1027	EST-SSR	GTCTCGGTCCGAACCATTTA	TCTTCTGATCAACAAAAGTAACAACA			IV	IV
292	S5	基因组SSR	TGTGGGGCTTGTTACACTGA	AGCTACCATAACAGACAAAACC	54	205	IV	IV
293	22276	基因组SSR	ATGCGGCATTTTGCTTTATC	TTGGTCTGCAAATCGAAACA	60	127	IV	IV
294	29630	基因组SSR	CCTACCTTAAGTCGCCCATGT	TCCAAACATAGGCTTCGTCTC			IV	IV	IV
295	AA285	锚定标记	TCGCCTAATCTAGATGAGAATA	CTTAACATTTTAGGTCTTGGAG	51	248	IV		IV
296	28304	基因组SSR	TTTTCAGCTGATCGGATATCTACA	GGCAGCATCTTGAAAATCGT	60	132	IV	IV	IV
297	e535	EST-SSR	AAAAACCAAGCACACCCATAA	GCAAGACACAGCACAAAAACA			IV	IV
298	27384	基因组SSR	TTGTGCCAACAAAAATCACAA	CAGAATGCCGTTCACTTTTCT	59	115	IV	IV
299	e561	EST-SSR	TCCGATCTTGCTTCTGAATCT	TTCATCAACCCAGACGCATA			IV	IV
300	B83	基因组SSR	CCCTCTCCCATCTCATCTCA	AAAGAAAGTAGAGATCCAGCACTGA	54	205	IV	IV
301	24635	基因组SSR	CCCTCTCCCATCTCATCTCA	AGAGATCCCAGCACTGATTG	58	152	IV	IV
302	23788	基因组SSR	TGGAGAAATTATGAGAATGTTCAATG	GCACGTCGACACACACAAAC	61	184	IV	IV
303	24392	基因组SSR	GCGGAAACAGGAGAGAGAGA	GCACGTGCTCCATCATAGAC	59	121	IV	IV
304	e828	EST-SSR	AACCCCATTTTCAATATTTTTCA	GGATTGTTCTCCGCATCTTC			IV	IV
305	18358	基因组SSR	CCTGAACCGATTTTGGTGAT	ATTCCGCCCTCTTTCACTTC	60	138	IV	IV
306	27275	基因组SSR	TGCTCACATTAACCAAAAGCAC	TGGATGGGTATGTCCCATTT	60	147	IV	IV	IV
307	27252	基因组SSR	TAATGCCGACTGTGTGCTGT	GCAATTCAGCAAAAAGGAGAA	59	111	IV		IV
308	AA122	锚定标记	GGGTCTGCATAAGTAGAAGCCA	AAGGTGTTTCCCCTAGACATCA	61	190	IV		IV
309	28244	基因组SSR	TGGGAGAGGGGATAACTGAA	CATGTTGTTTGGTGCGTTTC	59	182	IV		IV
310	19487	基因组SSR	CCACCTGCTCAATTCCAAAT	GGCGAAGCGAATCTAACATC	60	178	IV	IV	IV
311	22506	基因组SSR	CGAAACATGCACAACCATTT	TGAACGTTCTGACCCAGATG	59	208	IV	IV
312	22693	基因组SSR	CGACAACAACAACCACATCA	CTCCATCGAACGAAAGGAAC	59	148	IV	IV
313	24465	基因组SSR	TCAAGCAGAAGAGTCGACCA	TAGCTATGTTCCCGCCAAAT	60	161	IV	IV
314	25164	基因组SSR	CCAAATACAAGCATTAATAGGGAGA	TGGTCGACTCTTCTGCTTGA	60	110	IV	IV
315	25419	基因组SSR	TGCAAGTCCTGATGCAAGTC	GCGATTCAGGATTGGCTTAC	60	140	IV	IV
316	27605	基因组SSR	AAATGAACGGAAACAGAAAGAA	ACATAGCACACGCAGCAAAC	58	155	IV	IV
317	29578	基因组SSR	CAAGTCATGAACGTCTCAAAAGA	TGGACGCGTTTTAAAGTTCC			IV	IV
318	27854	基因组SSR	TCCTTCATCAAAACGCAACA	ATTGACGTTCAAGCGGGTAG	60	138	IV	IV
319	e155	EST-SSR	TTTCTCGTTGCACTCATCCA	TCGGTTGTCGTTTCTTGTTG	60	174	IV	IV
320	21866	基因组SSR	GCAGCCTTCAAATCCTCTTC	AAAACGCGCTTACGCTTCTA	59	139	IV	IV
321	29760	基因组SSR	TGTGCCTCAGAGATGTTCAAA	AGAGGTGGTGCGGTGACTAT			IV	IV
322	18049	基因组SSR	ACCCCTCTTTGCTAGGGTGA	ACCACACATCTCGCACACAT	60	202	IV	IV
323	e342	EST-SSR	CACAACAACCCCTCCAAAAC	TTTGGATTTTCGCTTGGGTA	60	189	IV	IV
324	e546	EST-SSR	TGACAGTGAGTGAGTGGCTTCT	TTGCGGGTGAAAAGAAAAAG			IV	IV
325	21805	基因组SSR	TGGGAATGTGAAGTGGTGAA	TGTGGTGTGGTTGGTTTCTG	60	128	IV	IV
326	21622	基因组SSR	ACAGCATGAAATGCGTGAAA	TCGTCATCCCAACTTCATCA	60	139	IV	IV	IV
327	AA315	锚定标记	AGTGGGAAGTAAAAGGTGTAG	TTTCACTAGATGATATTTCGTT	51		IV		IV
328	79	基因组SSR	GCTCAGTCAGCCCGTCATA	GTGCGTGTGTGCGTGTGT	60	66	IV		IV
329	17066	基因组SSR	CTCTCCCCCACACCTGATAA	GAGGACCCAGTAGGGATCGT	60	159	IV		IV
330	24036	基因组SSR	GAAGGACCAAATCAATTCTCTAAA	ACCGACGTCAACGACTGATA	58	196	IV	IV	IV
331	24423	基因组SSR	CATCCCACTCTAACCGCACT	GCATAATCGGCTCTCTCTCC	59	184	IV	IV
332	25717	基因组SSR	CGTGCATGCATGTGTATGTT	TCACCGATCAACACCAATTT	59	192	IV	IV
333	28434	基因组SSR	GTTTTCAATCGATCCGTCCA	TTCCACCGTCTTCTTCAACA	59	164	IV	IV
334	e1213	EST-SSR	TTGGTTTCCGGTTAAAATGA	CAATCCCATTCACACCACAA			IV	IV
335	e782	EST-SSR	CATTGAGTTTGAGGATGAGGA	CCCATAACCATATCTCACAGTTCA			IV	IV
336	e336	EST-SSR	CCCCAAACCATATCCCTACA	TTCCATTCCCAAACTCACTTG	60	170	IV	IV
337	21227	基因组SSR	CGGATTCAACAAGCAGAACA	CGAGAATGGAGGAAGAAGTTG	60	153	IV	IV
338	S236	基因组SSR	AAATGGCCGTTTTATGATCG	CGGAGCTGAACCTTCTGGTA	53	604	IV	IV
339	e182	EST-SSR	TGGTAACCCTAGCAATCATCA	CTCTTTGGCAACAACATCTCA	58	241	IV	IV
340	28173	基因组SSR	TGCATTGCTAATAACATTAGAACCAT	TTCCTTTTAAGCAAGGTGAGGT	59	197	IV	IV
341	29016	基因组SSR	TTTCAAAGGCAAGGCAAAAC	CACCTCGCAAAATTGGACTT			IV	IV
342	e273	EST-SSR	CAACAACTTCTACAGCAGCAA	GCAGTAGCATCTGGCTGTGA	57	152	IV	IV
343	e1121	EST-SSR	AACAACGGCAACAACAACAA	GTGGCCTTAGTCCCAAGAAA			IV	IV
344	e908	EST-SSR	TGCAGTGATGAAGTGGTTGA	CACTGCTCCATATCCCACAA			IV	IV
345	27057	基因组SSR	TGACCCTAGCAATTAGGATTTGA	ACCATGCCTCCAAAAACTTG	60	160	IV	IV
346	24907	基因组SSR	AAGCAATCCTAATCCATGTGTG	CATCCTTTCCGCCTTTGTTA	59	134	IV	IV	IV
347	22155	基因组SSR	ACCCGAGTCAGTCGCTTATG	AACACGGCTTCAATTTACGA	58	135	IV	IV
348	e878	EST-SSR	CGCATTTTCACTCCACACAC	CGTTCGGAACATCCAAGGT			IV	IV
349	o79	基因组SSR	TTGTCTTCACCACCTTAC	GATCATCAGCCAATAGTT	52	300	IV	IV
350	27068	基因组SSR	TTTCGGGCGTCAAATAATTC	GCCACACCTCCAAATGAGTT	60	152	IV	IV
351	20339	基因组SSR	CCTTCCGTGACCAAGAGAAA	GGTGGATGAGATGGATGAATG	60	146	IV	IV
352	e307	EST-SSR	CTTGTCAGCTTGGCATTCAA	GCGAGTTTCCATTCATCACA	60	177	IV	IV
353	e360	EST-SSR	CTTGCCCAAATTCAAGCTGT	CTCTATTACACAAATGCCAGTG	55	239	IV	IV
354	B110	基因组SSR	CCTCTTCAACGGTACGAGGA	TTGCAGAGAGACGAGAGAGAAA	56	208	IV	IV
355	27300	基因组SSR	CGGCAGTATTTGCAACAAGA	CCTCAAGGCCAGATGATTTT	59	159	IV	IV
356	e1029	EST-SSR	TCATTGCATGCCATTCTTTC	CGAAATAGAGAAAAGATAGAACCAG			IV	IV
357	29971	基因组SSR	CGAAATTGAATGCAGGAATG	CAACCTCCAAACTCCAAACAA			V		V
358	28387	基因组SSR	GGCTCCATATCATGTTTCTATGC	AAAAGGAGGGAACATGGAAGA	60	201	V	I-2	V
359	22059	基因组SSR	ATCTTCCGCAACAACACACA	ACGTGAAACGGCACAGTATT	58	203	V	I-2
360	27937	基因组SSR	ACAAGGCATGGTATGGTGGA	TGAACACAAATTGCAGCCTAA	59	124	V	I-2
361	S220	基因组SSR	AGCTCTTTCTTCCACCACCA	CAGGTTCCAGCTGAGAGGAG	55	1001	V	I-2
362	e59	EST-SSR	GAGGGTTTCCCGACTTCATT	TAAAGGTTTTCGCCACCATC	60	154	V	I-2
363	e723	EST-SSR	GGGGGTGTCTTACGTTGATG	CCCCAAAACCAGCTGAACTA			V	I-2
364	29094	基因组SSR	CCCCAAAACCAGCTGAACTA	GGGGGTGTCTTACGTTGATG			V	I-2
365	18391	基因组SSR	CCATCCTCCACGTGTCTCTT	TCGCATATCCAAATGCAAAC	60	142	V	I-2
366	e121	EST-SSR	AGCTCCATTTTGGAGTTTGT	CCTGAACCTGATTATAGCCAAGA	56	211	V	I-2
367	e619	EST-SSR	AAGTCTCTCATACCTAACCAACCAC	GCAGCCAAATTTGAGGAAGA			V	I-2
368	20702	基因组SSR	GTTCTCCATCGCCTTCTTTC	TGTGTTATGCCGAGCTTTTG	59	151	V	I-2
369	e611	EST-SSR	CCACAACCCCCTCTCTCTC	CTGCGAATTCGGAAAGAAAC			V	I-2
370	e1060	EST-SSR	AGAAGTTTTGTTGGTGCAAAGA	TGCTCATTTCTTTACCTTTCTTGA			V	I-2
371	e1233	EST-SSR	CGTTCCTTGTCTCTCCTCAAA	ATTCCCAACATGCACCATTT			V	I-2
372	22184	基因组SSR	GGGCGAAAACAACTTCCATA	CCTGGATGCTCCCAAAATAA	60	149	V	I-2
373	26117	基因组SSR	CATCGGGCGAGATAACAAAT	TTCCAAGCCTCACTTTCTCC	59	202	V	I-2
374	26857	基因组SSR	TGCTACAAGTCTAAATACAACACTCTT	CGGGAAGAGAATGATGAGGA	57	133	V	I-2
375	24882	基因组SSR	TTTCTGGAACCTCGCAAAAC	TTGCCTCAATTTGGAGACCT	60	173	V	I-2
376	23829	基因组SSR	CGCTCGGCCATGTAACTTAT	GGAAATGGGACTGAAACTGG	59	199	V	I-2	V
377	24112	基因组SSR	TTGATCATCCTCTCGCTTTT	TGTTGTCGTCATCAAAACACAG	57	187	V	I-2
378	23525	基因组SSR	TGTGCTTTTCTCTTGGCTTCT	CCAGAGGAACCACAAGGTGT	59	125	V	I-2
379	24512	基因组SSR	AAGCGTACGTGGCAAGAAAT	TCCCTGGGAGAGATGAAAGA	60	171	V	I-2
380	24236	基因组SSR	CAAACCTTCTTTATTTCCATTTCA	ACTTCTGGTCCACGCAAAAC	59	152	V	I-2
381	e738	EST-SSR	CCAATGGACTAGGTGGTGGA	TGATGGATGGGGTGATCATA			V	I-2
382	25394	基因组SSR	AATGGGTTTTGCTACGTGGT	GGGTGAGTGGAGAAAGCACT	59	142	V	I-2
383	25076	基因组SSR	GCTTGCAAGTGTGCGTGTAT	CCAGCCAAATGCACAATAAA	60	181	V	I-2
384	25618	基因组SSR	TTCCATCGTGAACCTTCCTC	CACACGACTTGCAATGTTCC	60	204	V	I-2	V
385	e122	EST-SSR	TCCACCGACATCTCTTCTCA	AGGTGGTGGTTGTTGTTGGT	59	180	V	I-2
386	19657	基因组SSR	TCCAAACCCTAGTTAGAGAAAGAA	AGCACCATCATCGTTCATCA	58	188	V	I-2
387	28929	基因组SSR	GGACTTTTGCGGGTATGAAA	TGTCTCTTTAGATTCGTTCCAAAA			V	I-2
388	e5	EST-SSR	ATTAGGGCCGGATAATTTGG	TCCTCAGCAGCTGTCTCAAA	60	162	V	I-2
389	25089	基因组SSR	ATTCTTGTTGGCGAAACACC	TTGCATTACCCAAAGCTCCT	60	185	V	I-2
390	24228	基因组SSR	GCAAATTTTCGTTAAATGGATGT	GACAACCTGGAGACGCATTC	59	134	V	I-2
391	26521	基因组SSR	TGTCTAAGGGTGACAAAAGATCA	TGAACCCGCTCTTCCTTACT	59	159	V	I-2
392	25851	基因组SSR	AGGCAACACGAGGACGAATA	CGACGGAATTGAAAAACAAAA	60	152	V	I-2
393	25986	基因组SSR	CAATAGGCCGCGTAAGAAAA	TTGCCATCGATTTGATTTGA	60	158	V	I-2	V
394	22848	基因组SSR	GTGGTGGAAGAGCGTTTGAT	CATGGTGCGTTAACCCAGTT	60	190	V	I-2
395	22829	基因组SSR	TAGAAGGTTGCCTTGGGTGT	GCCCACCAAAGAAATCAAAA	60	114	V	I-2
396	24850	基因组SSR	TGGCACACATCTTCAATACAAA	GCACAACCGTTTTTGGTTCT	59	137	V	I-2
397	26012	基因组SSR	TGGCCCTCAACCTTGTATGT	GCAACACAGAACAAAGCACAA	60	138	V	I-2
398	29460	基因组SSR	TTCCTGACGCGGACATTAAC	GGAAATTCGGCAAGGACTTA			V	I-2
399	e599	EST-SSR	GCAATTTTCTCACTCCATCTCC	AAAGGAAAGCAACTCGGTGA			V	I-2
400	29839	基因组SSR	GAACCTCGTTTTTGCATCCT	AATGATAGGGGTTGCCACAT			V	I-2
401	21936	基因组SSR	TGTTGTTTGTTTGGTTGAGGA	CGTTGGCAAACATCATTATCA	59	187	V	I-2
402	22325	基因组SSR	TGCGAGGGATGAGTTTCTTT	TGTGTGGCCAAATCGAAGTA	60	187	V	I-2	V
403	25721	基因组SSR	AAAAGGAAGCAACTCGGTGA	GCAATTTTCTCACTCCATCTCC	60	168	V		V
404	16452	基因组SSR	CGATGGTTGCTGTTGTGAGA	ACCCCAAACAAACACCAATG	61	129	V		V
405	24388	基因组SSR	GGTCTGGGTCTTTGGCCTAT	AGCATTGCAACGAAGGTTTC	60	126	V		V
406	23759	基因组SSR	GGGGTGACAGTGTAGGGTTTT	TAGGCACACGCTTTCATGTT	60	157	V	I-2	V
407	23282	基因组SSR	TGGTGATGCATGATCATTTAGA	TACAACCCCACCCTGATTGT	59	152	V		V
408	25755	基因组SSR	TTTTCCAACTAAGGTTGTTTCTTTC	CAAAAGGAGGAGGCTGAAGA	59	150	V		V
409	28889	基因组SSR	ATTTGTGGTGCAAGCCTTCT	AAAATTGTACATGGACTCCTTTCTC			V		V
410	27456	基因组SSR	GCACATCCCATTTTTCCAGT	TTCATTACTTTGATAGTGTTCACAAA	57	120	V		V
411	27661	基因组SSR	ATTCATCTCTTTTCCTAAACAAAAAT	CACACCTCCACGTTCATCTC	57	152	V		V
412	29062	基因组SSR	TCACCATCGTGAGCAAGTTC	GGATGTTACGCCCACAATG			V		V
413	29741	基因组SSR	GCAAAAAGCATTGTCCATTTC	GCCTAATCTACAAACGGCTGAG			V		V
414	PSGAPA1	锚定标记	GACATTGTTGCCAATAACTGG	GGTTCTGTTCTCAATACAAG	51		V		V
415	16914	基因组SSR	AACCTCGAGCAACAACAGGT	TTAGGTTGGCGTTTTTGGTC	60	149	V	I-2	V
416	e691	EST-SSR	GATTTAATGCGCGGTTGATG	TGAGTGAAATCATGGGTGGA			V	I-2
417	28482	基因组SSR	CCGACACACTCCTCAACAAA	TCATCAGGATGAGGACACTCC	60	155	V	I-2
418	29694	基因组SSR	GAGTGCCTGATCCAAGAGGA	CTCTAAAGGGTGGCAACGAC			V	I-2
419	28277	基因组SSR	AACACAAGCGCGTTAGTTGA	GACCAGAGTCGAAGCGAAAC	60	183	V	I-2
420	29430	基因组SSR	TGCGATTTTTCAGTGAGGTG	AACGCAGGTGATGAGCCTAT			V	I-2
421	27008	基因组SSR	CGAGCAACAGACTGCAAAAA	GCCAACTTTCAATGTTTGACATA	59	132	V	I-2
422	16549	基因组SSR	CAATGAGATGCTGGCGATAA	GTTCGGTGTTGTGGGTTTTT	60	140	V	I-2
423	e506	EST-SSR	CCCCTTTATCCCCCTATTTC	CCTCAACACCAATGAACCAC			V	I-2
424	e938	EST-SSR	CTCCTCCTCTGATCCCTTCA	AAATTTCGATCAGGGGTTCC			V	I-2
425	e625	EST-SSR	GCTCCAATGGCTTCCTAACA	AACAAGGGGCAATCACAATC			V	I-2
426	e800	EST-SSR	AATCGCCAAAGGGTTTGTTT	CGCTTTGGTTCTAGCAGGAT			V	I-2
427	e1095	EST-SSR	TATCCATTGCCAGCAGCATA	AATCGCCAAAGGGTTTGTTT			V	I-2
428	157	基因组SSR	CACATCGACAGAGACATACGA	GTGTGTGTGATGTGTGTGGTG	57	70	V	I-2
429	21491	基因组SSR	ACACGGGATCGAGCTTTAGA	TCCTTTCCTCTAACTTCTTCCTTCT	59	175	V	I-2
430	3644	基因组SSR	CACACGCAGAATCACACGTA	CGTGTGTGTGCATGTAATCG	59	176	V	I-2
431	3790	基因组SSR	GCGTGTGTACATGTGTGTGC	GCGACTTGCACAAGCAGA	60	182	V	I-2
432	4696	基因组SSR	TGCATGTGCATGTAATCGTG	TCACACGCACGTACAAATCA	60	213	V	I-2
433	5849	基因组SSR	ACATTCACACACACACGCAA	AAGCTGTGTGCACGTGAGTT	60	278	V	I-2
434	3919	基因组SSR	GCACACGCAAACTCACAAGT	TGCGTGTGTGCATTTGTTTA	60	186	V	I-2
435	919	基因组SSR	GCACACGCAAACTCACACTT	GCGCATGTGCATTCGTGT	60	94	V	I-2
436	4190	基因组SSR	ACACGCATGCACGATTACAT	TGTCCGTGTACGAGCTTTTG	60	194	V	I-2
437	4013	基因组SSR	ACACGCATGCACGATTACAT	GTACGAGCTTTTGTCACGCA	60	189	V	I-2
438	3772	基因组SSR	CATGCACGATTACATGCACA	CGTAGCTTTGTCACGCATGT	60	181	V	I-2
439	4156	基因组SSR	ACACGCATGCACGATTACAT	GTGTCGTGTACGAGCTTTGC	60	193	V	I-2
440	3835	基因组SSR	ACACGCATGCACGATTACAT	GCTTTGTCACGCGATGTGTA	60	183	V	I-2
441	4427	基因组SSR	ACACGCATGCACGATTACAT	CGTACGTGTCCGTGTACGAG	60	202	V	I-2
442	3955	基因组SSR	ACACGCATGCACGATTACAT	ACGTAGCTTTTGTCACGCAT	58	187	V	I-2
443	4014	基因组SSR	ACACGCATGCACGATTACAT	GTGTACGAGCTTTGTCACGC	60	189	V	I-2
444	3567	基因组SSR	TGCATATGTGTGTGTCTGCG	ATACACATGCGTGCAAAAGC	60	173	V	I-2
445	4274	基因组SSR	CACACAGAAGCACACGCC	GCATGTGTGTGTGCGATGTA	60	197	V	I-2
446	4043	基因组SSR	ACACGCATGCACGATTACAT	CGTGTACGTAGCTTTGCACG	60	190	V	I-2
447	3695	基因组SSR	ACACGCATGCACGATTACAT	TTGTCACGCATGTGTATGTGTT	60	178	V	I-2
448	3771	基因组SSR	ACACGCATGCACGATTACAT	GCTTTGTCACGCATGTGTATG	60	181	V	I-2
449	1683	基因组SSR	GTGCGCATGGTGCATATAAA	CATATACACACACGCACGCA	60	113	V	I-2
450	3807	基因组SSR	ACACGCATGCACGATTACAT	GCTTTGCACGCATGTGTAGT	60	182	V	I-2
451	4114	基因组SSR	ATACACGCATGGCACGATTA	GTACGAGCTTTTGTCACGCA	60	192	V	I-2
452	3600	基因组SSR	CACATGCACACGCACACTTA	TGTCCGTGTACGAGCTTTTG	60	174	V	I-2
453	4080	基因组SSR	ACACGCATGCACGATTACAT	TGTACGAGCTTTTGTCACGC	60	191	V	I-2
454	3770	基因组SSR	ACACGCATGCACGATTACAT	AGCTTTGCACGCATGTGTAT	59	181	V	I-2
455	3808	基因组SSR	ACACGCATGCACGATTACAT	GCTTTTGCACGCATGTGTAT	60	182	V	I-2
456	3921	基因组SSR	ACACGCATGCACGATTACAT	TACGAGCTTTTGTCACGCAC	60	186	V	I-2
457	3377	基因组SSR	CACATGCACACGCACACTTA	CGTAGCTTTGTCACGCATGT	60	165	V	I-2
458	3597	基因组SSR	CGGGTTCACGTATGTGTGTT	ACGCGTATATTCACACGCAC	59	174	V	I-2
459	3923	基因组SSR	TACACGCATGCACGATTACA	CGTAGCTTTGTCACGCATGT	60	186	V	I-2
460	4825	基因组SSR	GCATGTGCATTTAATCGTGC	TGCACACGTACACACAAATCA	60	220	V	I-2
461	3298	基因组SSR	TATGCGTGTGTGTGCTTGTG	CACACATACACGTGTGAACCC	60	162	V	I-2
462	3181	基因组SSR	CACATACACATGCGTGCAAA	CGTGTGGTCATGTACGTGTG	59	158	V	I-2
463	24331	基因组SSR	AATGGCGCACTTCACTTTCT	CCGTTAACGCCTAGCTCAAG	60	137	V	I-2
464	25387	基因组SSR	GGCTCATGCATCTACCACCT	ATCCCGACGTTCACATTTTC	60	170	V	I-2
465	24186	基因组SSR	GGTGGATCCTCCTTTTGTCA	TCCCAATCACCACTTCTTCA	59	167	V	I-2
466	19075	基因组SSR	CACGAGTACAACATGGAGTGAAG	CAAGCTCAACCTCCTCATACC	59	187	V	I-2
467	e531	EST-SSR	CACCTCCACCCTTTCACCT	CTGGAGGTGGGAGATTGTCT			V	I-2
468	28857	基因组SSR	CCGAAATGTTCCGAAGAGAG	TTTCAATTCAATGCCGAAAT			V	I-2
469	27046	基因组SSR	AAAGAAGGGGATGCGAGAAG	GCTCAAGTCAGTCGGACCAC	61	145	V	I-2
470	30087	基因组SSR	CGCATACACTGAGGTAACACC	AATACACCGGAAGAGGACCA			V	I-2
471	o65	基因组SSR	ACCGCAACAACAGGATAAT	TGAGGTGAAATCGGAAGAC	53	143	V	I-2
472	e1035	EST-SSR	TTTTGCACCCCCTTATGTCT	CCACAAAACTCGGGTGAAAT			V	I-2
473	19252	基因组SSR	CAATATTGATCGGAATTTGTTTC	TGCGGTTTGATTGAGTTTGA	58	199	V	I-2
474	29379	基因组SSR	AGGCACGTTGGTGCTAGACT	CCTCAATGATCCCAAAGCAC			V	I-2
475	e178	EST-SSR	CGAAGAAGATCAAAATCACCAC	AGCTTCAGGGGTTTCTTTCC	59	195	V	I-2
476	e135	EST-SSR	GTTCGTTCGTTCGTTCCTTG	TCTGTCTGGAAATGAAATGG	56	219	V	I-2
477	AD147	锚定标记	AGCCCAAGTTTCTTCTGAATCC	AAATTCGCAGAGCGTTTGTTAC	61	330	V	I-2	I
478	e921	EST-SSR	AAGGGGTGATCAAGCATCAA	TTGAGGGAACATGAAGAAATCA			V	I-2
479	e915	EST-SSR	GTGGACTCGGATTGGGACTA	GCATCGACGACGAAGAAGAC			V	I-2
480	111	基因组SSR	CGCACAGCAACACACACAT	GCAGTTAGTGCGTGCGTG	60	68	V	I-2
481	22913	基因组SSR	TCCAACAAACTCAGCCACAG	CAATGGTGGTGGTGCTCATA	60	175	V	I-2
482	23383	基因组SSR	TGGAGAAATTGGTGGTGACA	TGCAACCATGTTCTTGTTCC	60	141	VI	VI
483	23611	基因组SSR	TGCAAATGTGCAATGAATGA	GGCGGACATGAGAAGGAATA	60	187	VI	VI
484	25953	基因组SSR	GGCCACAACCGTGATGAG	GGATCCAAGACCGAGACAAC	60	117	VI	VI
485	24326	基因组SSR	AAAACGAGAGGCTCGAAACA	ACTAAAACCTCGCGCATCAC	60	185	VI	VI	VI
486	17422	基因组SSR	ACCACAAATGCTTCCGCTTA	GTTGTTGTTGCTGCTGCTGT	60	200	VI	VI
487	22197	基因组SSR	GTTGTTGTTGCTGCTGCTGT	ACCACAAATGCTTCCGCTTA	60	200	VI	VI
488	29622	基因组SSR	AACTTCTGCAGTGGCATGTG	CAAAACAACCTATAAGGATGGAAAA			VI	VI
489	29331	基因组SSR	GGGTGGACCGAATATTTCAA	CGTCACCTCTACCGAAGCTC			VI	VI
490	e124	EST-SSR	GCTTCTGAACCAAGCACACA	AACAATCCCATGTATCAGCAAC	59	235	VI	VI
491	e1097	EST-SSR	CCCTTCTCATGGGGAATGAT	TAGTCCATGGAAGCGGAAAA			VI	VI
492	e1105	EST-SSR	TAGTCCATGGAAGCGGAAAA	CCCTTCTCATGGGGAATGAT			VI	VI
493	27153	基因组SSR	GGGAGCGATGCACATAGTATT	GCCCTACAACGAGTGACACA	59	134	VI	VI
494	26929	基因组SSR	CACATTCACGACGAGGACAG	GCACACTGTAAGCACTTTTCTCA	60	142	VI	VI
495	e853	EST-SSR	CTTCCCGGGTAAGAACAACA	GCTATGGTTCAGGCGTTTCT			VI	VI
496	23637	基因组SSR	AAGAGGCTCGTGACCCAATA	TGCATTGCATCCTTCAAGAG	60	192	VI	VI
497	AD160	锚定标记	ACCAGTCAAATGGTTAGAAAGT	GAATGGAAAAGAGAATCAAGTT	51	190	VI	VI	VI
498	23578	基因组SSR	AAGGAAGGTGGTGTGGAATG	CAATATTACTCAGCCATTAATTAACCT	58	205	VI		VI
499	PSGSR1	锚定标记	TGAAACCACCATTCTCTGGA	AAGACCCCACTTGAAAATTACTTC	58		VI		VI
500	25075	基因组SSR	CATCACTCACTCGCCAAAAA	AACCATCTTTGCCAGGTACG	60	192	VI	VI	VI
501	e709	EST-SSR	TGTGCAACCGAGATTGGTAA	CGCCAAAAATACTGATTCACTTC			VI	VI
502	27176	基因组SSR	TGCCTTCAGGTTTTCAAGGT	TGATGAAAGCAATTTTCATGACTT	60	147	VI	VI
503	29246	基因组SSR	CCCTTGCTTGGGTAAGAAATC	GTGCCGGGTATGTATCTGGT			VI	VI	VI
504	4816	基因组SSR	CGTCATCATTGTTCGTCATTCT	GGTCGTAGGGTGTGTCGTCT	60	219	VI	VI
505	2089	基因组SSR	ACACACGTCACACACACGTC	TGATGTGTATGCGTGATGGA	59	124	VI	VI
506	28621	基因组SSR	CGTTTTCACATTCGCTAACC	TGGAGAAAGGTTTCCTGATGA	58	171	VI	VI
507	27428	基因组SSR	GCACGCCTGACTTCTTCTTT	AAATGGATTGCGACGTGATT	60	174	VI	VI
508	24560	基因组SSR	GATAAAGGCAGCGACAGAGG	AATGAAGTGCAAGCCCAAAT	60	202	VI	VI
509	23737	基因组SSR	CGTGCAACCATAGCAAGAGA	AAACCGCTCAAGCTCAGGTA	60	182	VI	VI
510	26025	基因组SSR	ATGCACTCAAAGGCCATCAT	CACTTGCAGAGCGAGAGAAA	59	113	VI	VI
511	24906	基因组SSR	TCGAGTCAATCGCTCAGAAC	TGCCCAGATGTCATAAGGTG	59	134	VI	VI
512	16445	基因组SSR	TCAAACCGCTGAAAAACAAA	GCGGTGGGAGGGAGATAC	59	128	VI	VI
513	16397	基因组SSR	AGGGCCAGGTTTATTTCCAC	TTTCCCAATGGCAAGTTAGC	60	123	VI	VI
514	e715	EST-SSR	CGTTGAAACAGCGATTCTGA	TTTCTTCAATACCTCAATGGTTC			VI	VI
515	28153	基因组SSR	TGGGTTGTCGTGTTGTTGTT	AACACTCCCAACTCCATTTTT	58	183	VI	VI
516	27491	基因组SSR	TCCTAACCAACCAATAACACGAT	TTGAGGATTTCGGTGACCTC	60	180	VI	VI
517	C58	基因组SSR	TCACGTGCTTGTCGTTCTTC	TAAGAAACCGCCATGGAT TT			VI	VI
518	B117	基因组SSR	ACATCAGGGAAGAACGCATC	GAGGGTGAAGACCAGCTTTG			VI	VI
519	30379	基因组SSR	TGTTGGCAGGAAACTCTTCA	AGCCACAAATTTCGTTGTGTT			VI	VI
520	24575	基因组SSR	TTGTGAGCACATTGGAGGAG	GGATTGTGTTGGTTAGAGAAAGAGA	60	161	VI	VI
521	23468	基因组SSR	CGGCAGCATCTACACAAGAG	ACGTTGAAGACTCCGTCACC	60	171	VI	VI
522	29440	基因组SSR	TGTTCCCCTTTAATTTTCATCCT	AAGAAGCCGTCACGAAATGT			VI	VI
523	30004	基因组SSR	TCTTTGCGGATATGCATTTTA	CGGGTGAGGACTGAAAACTC			VI	VI
524	2614	基因组SSR	ATGTGTGTGCGTGTGTGTTG	GATTGTTATGTGCTGCGTGG	60	140	VI	VI
525	3494	基因组SSR	GCACCGCTCTGACACTCATA	TGAGAGTGGAGTGGCTGAAG	59	170	VI	VI
526	3244	基因组SSR	CTTCCCCTCGCAATTTATGA	ATGTGTGTGCGTGTGTGTTG	60	160	VI	VI
527	4583	基因组SSR	ACACCATTGCACCATTCTGA	TGCGTGTGTTGTGAGTGAGA	60	208	VI	VI
528	4581	基因组SSR	ACACCATTGCACCATTCTGA	GTGCGTGTGTGTTGTGAGTG	60	208	VI	VI
529	4629	基因组SSR	ACACCATTGCACCATTCTGA	GTGCGTGTGTGTGTGAGTGA	60	210	VI	VI
530	4811	基因组SSR	ATGTGTGTGCGTGTGTGTTG	ACACCATTGCACCATTCTGA	60	219	VI	VI
531	e93	EST-SSR	ATGGCCTTTGCAATTACAGG	GCTGATGTTGGCCAAGGTAT	60	203	VI	VI
532	e771	EST-SSR	TCCGGCAAGATATTGGAAAA	CTGCAGAGGCTGTCACTCAA			VI	VI
533	e1109	EST-SSR	TCCGGCAAGATATTGGAAAA	GCTTGGATCGCAGGAAAATA			VI	VI
534	20896	基因组SSR	TGATGACCCTGCAAATTCAA	TGCACCACTGTCAGGTGATT	60	131	VI	VI
535	e596	EST-SSR	TCCCTCATTCTCCCTTTTCA	GACGGCGCTGATGATAGACT			VI	VI
536	e1098	EST-SSR	AGAGGACGTGTTGCTGTGTG	CACAGAATTGGCAGAAACAGAG			VI	VI
537	27272	基因组SSR	TGTAGCGGCACACTTTGAGA	GATCTCTGCCACCCATCTTC	60	190	VI	VI
538	28790	基因组SSR	GCTGTGGGGGTTTAATCAGA	CCGCAATCCTTCAAGAACTC	60	133	VI	VI
539	29200	基因组SSR	ATGCTGATGAAATGCGAATG	CATCTGTACCCGGACCTTTG			VI	VI
540	27844	基因组SSR	GCTTCAAGCTACCAAGTGGA	CCTCACGGGCTCTACCATAC	58	118	VI	VI
541	29877	基因组SSR	GTCGTGGGGAAAAGGTATCA	GGTACGACAACCCTACCTTTG			VI	VI
542	e975	EST-SSR	AGCAGCTCCTACTCCTTCTCC	GCGCAAATCCTATTCCAAAG			VI	VI
543	e940	EST-SSR	GCGCAAATCCTATTCCAAAG	ATTTCAGCAGCTCCTACTCCT			VI	VI
544	19691	基因组SSR	TTGTAAGACCGACTCGTCCA	CGGTCTGAGGTTGTTGTGAA	59	146	VI	VI
545	16588	基因组SSR	CGGTCTGAGGTTGTTGTGAA	TTGTAAGACCGACTCGTCCA	59	146	VI	VI
546	25488	基因组SSR	TGAAGAATGAGCTTCAATTTTTGT	GGGTGCAATCATGAGTGTTG	59	145	VI	VI
547	26625	基因组SSR	GCTCCATCACGGTGAGTTTT	TCCCACTTTCACGATGTTCA	60	191	VI	VI
548	25280	基因组SSR	CTCTCTGCCCACTGCTCTG	TCTCACGTTGGGATGCTAAA	59	122	VI	VI
549	25711	基因组SSR	AAGGTTTTGAAATAAATGAAGTTTG	TGAAAGCCCACTTGATCTTC	57	188	VI	VI
550	25888	基因组SSR	AAGGGGGAGAGAGGTGGTTA	TCGCCTTTTCTTTCTTCTTCA	59	164	VI	VI	VI
551	AA335	锚定标记	ACGCACACGCTTAGATAGAAAT	ATCCACCATAAGTTTTGGCATA	61	220	VI		VI
552	23568	基因组SSR	TCCCTCATTCTCCCTTTTCA	GACGGCGCTGATGATAGACT	60	127	VI	VI	VI
553	23081	基因组SSR	ACCCTTGCTTTGCCACATAA	TGTTGCTCTTTTGTTGAGTTGA	59	149	VI	VI
554	23431	基因组SSR	GCAACAACAGCAACCTCTGA	TGAAGGTGGAACTTGGTTTTG	60	145	VI	VI	VI
555	27435	基因组SSR	TTGATGCTCTTCTTCCATTCAA	CATACAAAACACACAAAAAGGATTG	60	171	VI		VI
556	16410	基因组SSR	AAGGTCATGCTTCTTCATCTCT	GGGTGAGGTGTTATGGCACT	57	125	VI		VI
557	28516	基因组SSR	CCAAAATTCATGCATGGTACG	TCCAGTGGCTCATAGAGGAGA	60	169	VI		VI
558	26140	基因组SSR	TTGTGTGCAAACCACCTAGC	GATTGCATCACACGGTCAAG	60	200	VI		VI
559	23309	基因组SSR	GAAGATGGCAACGTGTCAAA	AACTCATCCTCCACCACCAC	60	128	VI		VI
560	29872	基因组SSR	TCCACTTCCACCCACAAAAT	GCAATGGAGGTTTTGCTTTT			VI		VI
561	26514	基因组SSR	TGGGCACAAGTCTGTGAGAA	TTGGGTTGAGGTGTTTAGGTG	60	153	VI		VI
562	AD60	锚定标记	CTGAAGCACTTTTGACAACTAC	ATCATATAGCGACGAATACACC	51	216	VI		VI
563	AB71	锚定标记	CCAACCATTTGTGAGTTCCCTT	TTCGTCGAACCACGAGAATAGA	61	145	VI		VI
564	23949	基因组SSR	TCGGTCAATTTTCACGTAGC	GCAGAGAATGAAAGAAATATAAAGAAA	58	142	VI		VI
565	22903	基因组SSR	TGCTTGCCAGAATAAAAGTCC	CCTCTAGGGTTTCGGGTCTC	59	181	VI		VI
566	27055	基因组SSR	TCTGCAAACTCCCAACACTG	GGTGGTTTGGTTGCAATCTT	60	206	VII	VII
567	e282	EST-SSR	GGCAAGCATAAAAGGGACAC	TTCATCCAAGAACCCTCGAC	60	185	VII	VII
568	e144	EST-SSR	TCCATTTCCCGAGTTTATCG	AACACGAATATGCAACAAGC	56	151	VII	VII
569	S244	基因组SSR	TTTAGCACAGAACAGCGTAGT	TAACGCCCTTGAGAATTTCG	53	606	VII	VII
570	21399	基因组SSR	TGATTCTAGTTCATTTCACAAACACA	CGTCCTGCACCTAGCTTCTT	60	153	VII	VII	VII
571	26099	基因组SSR	GCTCTTCGTAACGCTCACAA	TGACGACGGAGACTGAGTTG	59	201	VII		VII
572	19979	基因组SSR	CACCAGAAAATTTGTTATCAAAAAGA	GCACCCTGGGAAATTACAAA	60	161	VII	VII	VII
573	24547	基因组SSR	CGGCAGAATTAGGGTTTTGA	TCAATTCCGAACCACCTTTC	60	198	VII	VII
574	e968	EST-SSR	ACCGCTTGAACTCCAAACAA	GAAGGTAACAACGCCGAGAA			VII	VII
575	18013	基因组SSR	TCAATTCCGAACCACCTTTC	CGGCAGAATTAGGGTTTTGA	60	198	VII	VII
576	S144	基因组SSR	TTTTCTCACCGCGCTTATTT	AACAACCACCGAAGACGAAG	54	235	VII	VII
577	20828	基因组SSR	CATGGATCCCAACAGAAACA	TGGTTTTTACCCGAGACTGG	59	144	VII	VII
578	e876	EST-SSR	GCAGATTGACTGCTCATGATGT	AGCTGATTATTGGGCACCTG			VII	VII
579	21405	基因组SSR	TCCACCATCTAATCCCCTCTT	AGCTGATTATTGGGCACCTG	60	147	VII	VII	VII
580	18103	基因组SSR	CATGTGCATGTGCAAGTACG	CTTTAAAATGCCCGGACAAA	60	209	VII		VII
581	17431	基因组SSR	TTCACAATTCACCACCAATCA	CCAACGTCAGGTACGATTCA	60	201	VII	VII	VII
582	17666	基因组SSR	GTGCATTGGCTCGTACTCAA	TCCACAATATAGCCCAGACCA	60	158	VII	VII	VII
583	S85	基因组SSR	TTCCAACCATGGAAGCTTTT	TTCTTCGTCGGGTACAGTGA	54	188	VII	VII
584	e255	EST-SSR	TGGAGAAGGGCAAAATATCG	TCTCAACACCACATCAAGGAA	59	249	VII	VII
585	25965	基因组SSR	TGATTCGTAGACCCCACACA	AAGGTTAATGTCTTCTTTTTGAAGTT	57	150	VII	VII	VII
586	17384	基因组SSR	AGTAGCGGTGTGTGGTTGTG	GGGAAGAAAAAGGTTGGAAGA	60	197	VII	VII	VII
587	30253	基因组SSR	CTACGTTTGGCCCTTGTGTT	GGCCCTAAATCTAAAATGAACAA			VII	VII
588	B179	基因组SSR	ACCGACGCTTCAATGGTATT	TTCATCTCCGACCCTACACC	55	267	VII	VII
589	e499	EST-SSR	GACTCCACGCACAGAACTGA	GGAGAGGGGAGTGAATGTGA			VII	VII
590	e544	EST-SSR	CACTACACAAGAAGCAAAGAAAAA	ATTCCTTTCCGGTCCATTTC			VII	VII
591	e1002	EST-SSR	CCACGCGTGACAAGTAAAGA	ATTCCTTTCCGGTCCATTTC			VII	VII
592	21420	基因组SSR	GGTGGTCGACGTATCGAAGT	TCAATGTTGTTGCGCTTACAT	59	142	VII	VII
593	18255	基因组SSR	TGTCAAATCCAATAAAAACACACA	TTTGTGCACACCGTCAATTT	59	129	VII	VII
594	e643	EST-SSR	CACCACCACTCTCACACCAT	TGCATTGCGAGAGTAAGACAA			VII	VII
595	e825	EST-SSR	AGTTTCCGCCATCAACATTT	CCTACCTGCATTCACAACCAT			VII	VII
596	17225	基因组SSR	GTTGCAAGCTGCTACCATCA	AGACGGATCCAACAATCTCC	59	182	VII	VII
597	17593	基因组SSR	CATCCTCCTCCTCCATACCA	TCATCATCAATGCAAAGGACA	60	148	VII	VII
598	24407	基因组SSR	GGGATCAAAGCAACCCTTTT	CATGGCAAGGAAGACCGTAG	60	208	VII	VII
599	24810	基因组SSR	TGAGCGAGGTAGGAAGAACC	CCTCTACAGTGGCCCTCTCA	59	132	VII	VII
600	25354	基因组SSR	ACCCTTGGGGCTTACAATTC	GACGTGGCTGGACATAACAA	60	184	VII	VII
601	25799	基因组SSR	CTGCAGAAGGCCCTGTTCTA	GATTCTTCATTCTCAACACACATTG	60	137	VII	VII
602	25430	基因组SSR	GCACTTGACGATGCATTTGA	GGGAAAGGGAACGATTTCAT	60	210	VII	VII
603	23294	基因组SSR	GGAGGAGGATGACGATGAAA	AGGGCTACCGGACTGAAACT	60	172	VII	VII
604	24142	基因组SSR	GCAGCCATGGTTGATTGATT	TCAAGAACATTACTTTTTCCCTCT	58	194	VII	VII
605	27051	基因组SSR	TGGTGGTGGAGAGTGATTGA	TCTGGTGGTACTTCCTCCAAAT	60	208	VII	VII
606	e1114	EST-SSR	GTGCGGCTTCATTTTCAACT	TTCTCAACTGGTTGGTTCCATA			VII	VII
607	e1209	EST-SSR	AGTGCGGCTTCATTTTCAAC	TCAATTTGATCCATGCAGTAGG			VII	VII
608	AF004843	锚定标记	CCATTTCTGGTTATGAAACCG	CTGTTCCTCATTTTCAGTGGG	54		VII	VII
609	23606	基因组SSR	GGGTTTGCCTCTTTTTCTCTC	ATCGTCAAAACTGCCCAAAC	59	113	VII	VII
610	21345	基因组SSR	TGCAATGCATGTTGATACGTC	GCAAAAACTCAAACTCAAACTCAA	60	152	VII	VII
611	27583	基因组SSR	TGCACAGAGGATGGTTCTCA	TGGATTGAGCCTCTTGTCCT	60	110	VII	VII
612	25610	基因组SSR	TTTGGTCGTTGCCAAACTAA	AGGACATACCGAGCCAGATG	59	133	VII	VII
613	26656	基因组SSR	AGAAGAGCGTCGGGAAGAGT	CCATGACGGAAAACAACCTT	60	188	VII	VII
614	25728	基因组SSR	TGGACAAATCTCGTGCAAAG	CCAACTTCCCATCTCCAACA	60	164	VII	VII
615	24471	基因组SSR	CAACACAACCTCCTCCAGGT	TAAGCCATTCCCACCTTCTG	60	121	VII	VII
616	24734	基因组SSR	CGCATGAGAGGATAATGATGAA	CCATAGCTTTCCCGAATCAG	60	183	VII	VII
617	24588	基因组SSR	AAATAGATGAGAAAGAGAGAGATTACG	CGCACTTCCATTCACATGAT	57	137	VII	VII
618	24602	基因组SSR	TGAGTGGGCGTGTGATTTAG	TTGCACTGTCGCATTTGAGT	60	118	VII	VII
619	24503	基因组SSR	AAAGGAGATCACCTATGAGAGAGAA	AATTGATTTGGGATCTTGGATA	57	154	VII	VII	VII
620	28097	基因组SSR	CAAAATCGGCAGGATTTACAA	TTTGCCTATGAACCTAAAACCAT	59	124	VII		VII
621	24814	基因组SSR	GTGCAACCAGAGCATGTTTC	CATCGACTGTGGAGACATTGA	59	152	VII		VII
622	AB65	锚定标记	CTCGTATCCAAAGATTCGTAGA	AGGGTTAATCGGAGTTTTATGA	51		VII		VII
623	29468	基因组SSR	AGTACTCTCGCCGCATCAGT	CACCCCACTTGAGCATATCA			VII	VII	VII
624	28967	基因组SSR	CTCGTCCTCATCGAAAAGCTA	GTGAACAACGCAAGGGTTTC			VII	VII
625	29500	基因组SSR	TGGGAATTGACGAAGAGTGTT	ATGGAGAGGGTTGCTGACAT			VII	VII
626	e46	EST-SSR	AGGAGGGAGTGGTGGAGATT	CATCACGTGCTTGTGCTTCT	60	227	VII	VII
627	e1173	EST-SSR	TGATTCCGAATGGGAAACTT	AATCCGCAAACACATCAACA			VII	VII
628	e1276	EST-SSR	TGAAACAATAGTGCTTTGTTGAAACT	TTTTCTCGTCTGCGTGTGAC			VII	VII
629	22060	基因组SSR	CCGCCTTAGGAAGCCTAACT	CGGCTTGATAATTTGGTGCT	60	181	VII	VII
630	e628	EST-SSR	GCCACCCTGTTTCTGCTAAC	CTTGTGGATCGGTTCGTGAT			VII	VII
631	e524	EST-SSR	TCACACCATAGAGAATAACAACAACA	TCTGAAGCCATCTCCATTCTC			VII	VII
632	28608	基因组SSR	TCATGATTTCAAATTTCTTTCACAA	CGCCGTTGGGTAATTGTAAC	60	136	VII	VII
633	22101	基因组SSR	ACCAATCCAGACGCAAATTC	GGGGACAGTGACGAGAACAT	60	156	VII	VII
634	19460	基因组SSR	CAATCCAAACGCAAATCTAACA	AATTGCAAGCCCTACACACA	59	187	VII	VII
635	17910	基因组SSR	CATGCCTGCTTCCTTCTGTT	TTGCAATTTCAAGCCTTCAC	59	187	VII	VII	VII
636	27398	基因组SSR	GAACCACATTGGGGATTCTG	AACCTGCAAAAGCCATAAGC	59	180	VII	VII
637	30165	基因组SSR	CCTTTTTACCCCTCCCTCAG	TCAAATGCAAAGGGAAACAA			VII	VII
638	27755	基因组SSR	TGCATTTGAGCTAGTGACATTCT	AAAAACCAACCCAACCACTT	58	163	VII	VII
639	e1193	EST-SSR	GTTGGCGAGGAATGATTGTT	TCACACACTCTGCCATTTCA			VII	VII
640	PSAB60	基因组SSR	AATTAATGCCAATCCTAAGGTATT	GGTTGCACTATTTTCGTTCTC	59		VII	VII
641	28054	基因组SSR	AGCAAAGTCACCAGCTGTTTT	TCCTGTTCTAAAACAAAAACAAGAGA	59	113	VII	VII
642	30124	基因组SSR	GACTATGTGTGTGCATGAATTTGA	GGCCATCTCGTTTCAGTACC			VII	VII
643	30304	基因组SSR	CACGCACAGACAGATCATCA	GAAGTTGGGGATGGGAAGAT			VII	VII
644	28108	基因组SSR	CGACAATGTTGCCAGCTATC	TTTTAGGATTTTATCGACGTTTTTC	59	119	VII	VII
645	e291	EST-SSR	CAAGCGTCGAAGATGAACAA	GCTGGCTGCAAAAGTTTACC	60	205	VII	VII
646	e292	EST-SSR	GCACATGAAAAATGCCAAAG	CTGTTGCTGTTGGTGGTGAG	59	220	VII	VII
647	PS11824	基因组SSR	ACCACCACCACCGAGAAGAT	TTTGTGGCAATGGAGAAACA	52	210	VII	VII
648	e527	EST-SSR	TTGAAGCAGTGGCAGAGTTG	TCTCAATGAAACATAAGAATGACCTT			VII	VII
649	e1202	EST-SSR	TGCATGGTTATGATGCTTGA	TCACACACCCCTTCAATTATTC			VII	VII
650	23521	基因组SSR	CGCCAATTCCTTTTCCCTAC	AGAACTCACAGGCGATGGTC	60	112	VII	VII
651	24652	基因组SSR	GAGAAAGCGGCTGCTTAGAA	GCTGTCACCGAGAATGATGA	60	190	VII	VII
652	25241	基因组SSR	TGCAAGCAAGCAAAATTGAA	TGCATGCCCTTTATTTCTTTG	60	142	VII	VII
653	24944	基因组SSR	TCAACCGGAATCTGGAAAAC	ATGGCGCAATCCTAGTGAAC	60	193	VII	VII
654	22896	基因组SSR	ATGTACGCCATGCAGTCAAA	ACAAGATGGGCGTCGAATAC	60	151	VII	VII
655	24398	基因组SSR	TTCGATGCATGAATGACAAA	ATCGGCGGAGACTAAGATCA	59	142	VII	VII
656	20817	基因组SSR	GGCGTAGAGGGCTAAACCTT	TTCCCGAGTCCTAACTTTCTTG	60	135	VII	VII
657	19526	基因组SSR	ACTCCTGGACACCCTGAGAA	CTGACCAAGGGGACCTGTAA	60	198	VII	VII
658	29620	基因组SSR	CCACTGAAGGCTCCTGAACT	AGCGATCACCGATAGTGTCC			VII	VII	VII
659	e1220	EST-SSR	ATGGTGGTGGAGGTGTGATT	CATCGCCAAATGGATCTTCT			VII	VII
660	17980	基因组SSR	CAATTCACAACGTTCCACTCA	TTTTCGTGAAATTGAAATGACC	59	194	VII	VII	VII
661	29248	基因组SSR	CAACAATGTGCATGGAAAAA	CTCGCATTGCGTAACGATAA			VII	VII	VII
662	28595	基因组SSR	CCGGTTCATCGATAAATGTGA	TCTCAAACCCACCAACAACA	60	199	VII	VII
663	2629	基因组SSR	CAAAACACATACGCACACACA	CCCGTCATGATGTCATGTAAA	59	141	VII	VII
664	e123	EST-SSR	GAGCACAACTTTGCAAGCAG	ACACGTCATTTCAAACCACT	55	198	VII	VII
665	e1249	EST-SSR	CCCGTTTCAAATTAGAACGATAA	CACGGTTCGGCATTTATTTC			VII	VII
666	23536	基因组SSR	GACGTTGATTGGCCTGTTTT	TGACCATGACATGCCTGTTT	60	180	VII	VII
667	21796	基因组SSR	TCTTCGCTGGGAAGTTGAGT	GGAAGCGATGTCGTTTCATT	60	210	VII	VII	VII
668	28261	基因组SSR	CTCTCCCCATGGAGAACAAC	AACAGCTGAAATTGGCGTAGA	60	131	VII		VII

Supplementary table 1

Supplementary Fig. 1

Supplementary table 2

BLAST results of common markers on genetic linkage maps of pea"

标记名称Marker name	连锁群Linkage group			染色体/连锁群 Chromosome/LG	比对条数 Number of blast results	最小E-value The minimal of E-value	最高比对率 The maximal identifies (%)	起始位置 Start position	终止为止 End position	比对长度Mapped length
标记名称Marker name	整合图谱 Integrated map	PSP1 G0003973 × G0005527	PSP2 W6-22600×W6-15174)	染色体/连锁群 Chromosome/LG	比对条数 Number of blast results	最小E-value The minimal of E-value	最高比对率 The maximal identifies (%)	起始位置 Start position	终止为止 End position	比对长度Mapped length
20229	I	I	I	chr2LG1	20	6.7E-94	100	21,564,671	21,564,851	180
25334	I	I	I	chr2LG1	4	2.7E-70	100	329,941,758	329,941,897	139
23261	I	I	I	chr2LG1	1	1.7E-79	100	410,201,631	410,201,786	155
24301	II	II	II	chr6LG2	1	3.9E-58	96	44,747,008	44,747,143	135
28257	II	II	II	chr6LG2	1	3.5E-55	93	89,359,789	89,359,941	152
17754	II	II	II	chr6LG2	1	3.0E-85	99	284,992,981	284,993,149	168
25769	II	II	II	chr6LG2	17	3.9E-58	95	379,342,213	379,342,360	147
25792	II	II	II	chr6LG2	3	6.5E-121	93	430,274,319	430,274,608	289
26850	III	III	III	chr5LG3	1	4.5E-80	99	95,945,216	95,945,375	159
21726	III	III	III	chr5LG3	2	4.4E-80	100	105,392,839	105,392,995	156
25151	III	III	III	chr5LG3	31	4.4E-80	100	128,426,677	128,426,833	156
18135	III	III	III	chr5LG3	1	8.3E-59	100	157,545,697	157,545,816	119
22754	III	III	III	chr5LG3	3	2.2E-82	100	178,517,012	178,517,172	160
20075	III	III	III	chr5LG3	1	6.6E-45	95	255,763,443	255,763,559	116
29630	IV	IV	IV	chr4LG4	1	2.3E-97	100	77,755,735	77,755,921	186
28304	IV	IV	IV	chr4LG4	4	5.8E-64	99	77,763,197	77,763,328	131
27275	IV	IV	IV	chr4LG4	5	1.1E-58	94	144,374,211	144,374,362	151
24036	IV	IV	IV	chr4LG4	1	1.5E-102	100	298,729,278	298,729,473	195
19487	IV	IV	IV	chr4LG4	2	3.6E-92	100	329,433,230	329,433,407	177
21622	IV	IV	IV	chr4LG4	1	1.0E-69	100	411,138,053	411,138,191	138
24907	IV	IV	IV	chr4LG4	1	7.5E-67	100	441,578,306	441,578,439	133
23829	V	V	V	chr3LG5	4	2.8E-104	100	40,897,218	40,897,416	198
25618	V	V	V	chr3LG5	10	8.7E-101	99	91,875,171	91,875,372	201
25986	V	V	V	chr3LG5	1	9.0E-56	91	181,332,864	181,333,028	164
16914	V	V	V	chr3LG5	1	2.2E-45	89	191,073,596	191,073,747	151
22325	V	V	V	chr3LG5	1	5.4E-91	98	221,771,656	221,771,845	189
23759	V	V	V	chr3LG5	1	4.4E-80	100	276,140,552	276,140,708	156
24326	VI	VI	VI	chr1LG6	1	3.3E-96	100	30,662,379	30,662,563	184
25075	VI	VI	VI	chr1LG6	1	1.0E-92	98	46,237,147	46,237,342	195
29246	VI	VI	VI	chr1LG6	2	5.0E-72	100	63,898,496	63,898,638	142
23568	VI	VI	VI	chr1LG6	1	7.9E-63	100	339,601,745	339,601,871	126
25888	VI	VI	VI	chr1LG6	1	4.1E-84	100	342,366,936	342,367,099	163
23431	VI	VI	VI	chr1LG6	1	3.5E-73	100	359,154,849	359,154,993	144
21405	VII	VII	VII	chr7LG7	1	4.0E-47	90	23,151,033	23,151,166	133
17431	VII	VII	VII	chr7LG7	2	7.5E-94	98	28,469,955	28,470,153	198
19979	VII	VII	VII	chr7LG7	1	8.3E-71	97	33,247,383	33,247,539	156
17666	VII	VII	VII	chr7LG7	1	8.1E-71	97	33,247,466	33,247,619	153
25965	VII	VII	VII	chr7LG7	1	7.5E-49	89	79,270,351	79,270,512	161
17384	VII	VII	VII	chr7LG7	1	4.0E-103	100	86,051,350	86,051,546	196
29468	VII	VII	VII	chr7LG7	1	3.9E-58	95	196,186,432	196,186,576	144
24503	VII	VII	VII	chr7LG7	4	2.6E-52	91	201,461,481	201,461,642	161
17910	VII	VII	VII	chr7LG7	1	2.0E-68	97	329,043,834	329,043,990	156
17980	VII	VII	VII	chr7LG7	1	1.9E-94	98	474,504,396	474,504,591	195
29248	VII	VII	VII	chr7LG7	1	7.2E-75	99	481,640,880	481,641,037	157
21796	VII	VII	VII	chr7LG7	1	2.7E-97	97	488,671,955	488,672,158	203
18272	II	II	II	scaffold00024	1	9.2E-59	98	43,943	44,076	133
23262	III	III	III	scaffold03071	1	4.7E-95	100	151,557	151,739	182
28387	V	V	V	scaffold03509	2	2.0E-105	100	40,828	41,028	200
21399	VII	VII	VII	scaffold00916	1	8.8E-67	96	9,846	10,002	156
29620	VII	VII	VII	scaffold01087	12	1.1E-54	96	47,702	47,832	130

Supplementary table 2

References 65

[1]	Zong X X, Redden R J, Liu Q C, Wang S M, Guan J P, Liu J, Xu Y H, Liu X J, Gu J, Yan L, Ades P, Ford R. Analysis of a diverse global Pisum sp collection and comparison to a Chinese local P. sativum collection with microsatellite markers. Theor Appl Genet, 2009,118:193-204. doi: 10.1007/s00122-008-0887-z
[2]	Smýkal P, Aubert G, Burstin J, Coyne C J, Ellis N T H, Flavell A J, Ford R, Hýbl M, Macas J, Neumann P, McPhee K E, Redden R J, Rubiales D, Weller J L, Warkentin T D. Pea (Pisum sativum L.) in the genomic era. Agronomy, 2012,2:74-115. doi: 10.3390/agronomy2020074
[3]	Tian S J, Kyle W S A, Small D M. Pilot scale isolation of proteins from field peas (Pisum sativum L.) for use as food ingredients. Int J Food Sci Technol, 1999,34:33-39. doi: 10.1046/j.1365-2621.1999.00236.x
[4]	Santalla M, Amurrio J M, De Ron A M. Food and feed potential breeding value of green, dry and vegetable pea germplasm. Can J Plant Sci, 2001,81:601-610. doi: 10.4141/P00-114
[5]	FAOSTAT. [2020-03-14]. http://www.fao.org/faostat/en/#data.
[6]	MacWilliam S, Wismer M, Kulshreshtha S. Life cycle and economic assessment of Western Canadian pulse systems: The inclusion of pulses in crop rotations. Agric Syst, 2014,123:43-53. doi: 10.1016/j.agsy.2013.08.009
[7]	Semagn K, Bjornstad A, Ndjiondjop M N. Principles, requirements and prospects of genetic mapping in plants. Afr J Biotechnol, 2006,5:2569-2587.
[8]	Dirlewanger E, Isaac P G, Ranade S, Belajouza M, Cousin R, de Vienne D. Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. Theor Appl Genet, 1994,88:17-27. doi: 10.1007/BF00222388 pmid: 24185876
[9]	Laucou V, Haurogne K, Ellis N, Rameau C. Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum. Theor Appl Genet, 1998,97:905-915. doi: 10.1007/s001220050971
[10]	Loridon K, McPhee K, Morin J, Dubreuil P, Pilet Nayel M L, Aubert G, Rameau C, Baranger A, Coyne C, Lejeune Henaut I, Burstin J. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor Appl Genet, 2005,111:1022-1031. doi: 10.1007/s00122-005-0014-3
[11]	DeCaire J, Coyne C J, Brumett S, Shultz J L. Additional pea EST-SSR markers for comparative mapping in pea (Pisum sativum L.). Plant Breed, 2012,131:222-226. doi: 10.1111/pbr.2011.131.issue-1
[12]	Mishra R K, Gangadhar B H, Nookaraju A, Kumar S, Park S W. Development of EST-derived SSR markers in pea (Pisum sativum) and their potential utility for genetic mapping and transferability. Plant Breed, 2012,131:118-124. doi: 10.1111/pbr.2011.131.issue-1
[13]	Leonforte A, Sudheesh S, Cogan N O I, Salisbury P A, Nicolas M E, Materne M, Forster J W, Kaur S. SNP marker discovery, linkage map construction and identification of QTLs for enhanced salinity tolerance in field pea (Pisum sativum L.). BMC Plant Biol, 2013,13:161. doi: 10.1186/1471-2229-13-161 pmid: 24134188
[14]	Sindhu A, Ramsay L, Sanderson L A, Stonehouse R, Li R, Condie J, Shunmugam A S K, Liu Y, Jha A B, Diapari M, Burstin J, Aubert G, Tar’an B, Bett K E, Warkentin T D, Sharpe A G. Gene-based SNP discovery and genetic mapping in pea. Theor Appl Genet, 2014,127:2225-2241. doi: 10.1007/s00122-014-2375-y
[15]	Sun X L, Yang T, Hao J J, Zhang X Y, Ford R, Jiang J Y, Wang F, Guan J P, Zong X X. SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties. Crop J, 2014,2:170-174. doi: 10.1016/j.cj.2014.03.004
[16]	Tayeh N, Aluome C, Falque M, Jacquin F, Klein A, Chauveau A, Berard A, Houtin H, Rond C, Kreplak J, Boucherot K, Martin C, Baranger A, Pilet-Nayel M L, Warkentin T D, Brunel D, Marget P, Le Paslier M C, Aubert G, Burstin J. Development of two major resources for pea genomics: the GenoPea 13.2K SNP Array and a high-density, high-resolution consensus genetic map. Plant J, 2015,84:1257-1273. doi: 10.1111/tpj.13070 pmid: 26590015
[17]	Boutet G, Carvalho S A, Falque M, Peterlongo P, Lhuillier E, Bouchez O, Lavaud C, Pilet-Nayel M L, Riviere N, Baranger A. SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population. BMC Genomics, 2016,17:121. doi: 10.1186/s12864-016-2447-2 pmid: 26892170
[18]	Ma Y, Coyne C J, Grusak M A, Mazourek M, Cheng P, Main D, McGee R J. Genome-wide SNP identification, linkage map construction and QTL mapping for seed mineral concentrations and contents in pea (Pisum sativum L.). BMC Plant Biol, 2017,17:43. doi: 10.1186/s12870-016-0956-4 pmid: 28193168
[19]	Barilli E, Cobos M J, Carrillo E, Kilian A, Carling J, Rubiales D. A high-density integrated DArTseq SNP-based genetic map of Pisum fulvum and identification of QTLs controlling rust resistance. Front Plant Sci, 2018,9:167. doi: 10.3389/fpls.2018.00167 pmid: 29497430
[20]	Kreplak J, Madoui M A, Capal P, Novak P, Labadie K, Aubert G, Bayer P E, Gali K K, Syme R A, Main D, Klein A, Berard A, Vrbova I, Fournier C, d’Agata L, Belser C, Berrabah W, Toegelova H, Milec Z, Vrana J, Lee H, Kougbeadjo A, Terezol M, Huneau C, Turo C J, Mohellibi N, Neumann P, Falque M, Gallardo K, McGee R, Tar’an B, Bendahmane A, Aury J M, Batley J, Le Paslier M C, Ellis N, Warkentin T D, Coyne C J, Salse J, Edwards D, Lichtenzveig J, Macas J, Dolezel J, Wincker P, Burstin J. A reference genome for pea provides insight into legume genome evolution. Nat Genet, 2019,51:1411-1422. doi: 10.1038/s41588-019-0480-1 pmid: 31477930
[21]	Kalia R K, Rai M K, Kalia S, Singh R, Dhawan A K. Microsatellite markers: an overview of the recent progress in plants. Euphytica, 2011,177:309-334. doi: 10.1007/s10681-010-0286-9
[22]	Vieira M L C, Santini L, Diniz A L, Munhoz C D. Microsatellite markers: what they mean and why they are so useful. Genet Mol Biol, 2016,39:312-328. doi: 10.1590/1678-4685-GMB-2016-0027 pmid: 27561112
[23]	Ali A, Pan Y B, Wang Q N, Wang J D, Chen J L, Gao S J. Genetic diversity and population structure analysis of Saccharum and Erianthus genera using microsatellite (SSR) markers. Sci Rep, 2019,9:10. doi: 10.1038/s41598-018-36877-0 pmid: 30626881
[24]	Hao L, Zhang G S, Lu D Y, Hu J J, Jia H X. Analysis of the genetic diversity and population structure of Salix psammophila based on phenotypic traits and simple sequence repeat markers. PeerJ, 2019,7:e6419. doi: 10.7717/peerj.6419 pmid: 30805247
[25]	Choi J K, Sa K J, Park D H, Lim S E, Ryu S H, Park J Y, Park K J, Rhee H I, Lee M, Lee J K. Construction of genetic linkage map and identification of QTLs related to agronomic traits in DH population of maize (Zea mays L.) using SSR markers. Genes Genomics, 2019,41:667-678. doi: 10.1007/s13258-019-00813-x pmid: 30953340
[26]	Yang T, Jiang J Y, Zhang H Y, Liu R, Strelkov S, Hwang S F, Chang K F, Yang F, Miao Y M, He Y H, Zong X X. Density enhancement of a faba bean genetic linkage map (Vicia faba) based on simple sequence repeats markers. Plant Breed, 2019,138:207-215. doi: 10.1111/pbr.2019.138.issue-2
[27]	Anjani K, Ponukumatla B, Mishra D, Ravulapalli D P. Identification of simple-sequence-repeat markers linked to Fusarium wilt (Fusarium oxysporum f. sp carthami) resistance and marker- assisted selection for wilt resistance in safflower (Carthamus tinctorius L.) interspecific offsprings. Plant Breed, 2018,137:895-902. doi: 10.1111/pbr.2018.137.issue-6
[28]	Swathi G, Rani C V D, Md J, Madhav M S, Vanisree S, Anuradha C, Kumar N R, Kumar N A P, Kumari K A, Bhogadhi C, Ramprasad E, Sravanthi P, Raju S K, Bhuvaneswari V, Rajan C P D, Jagadeeswar R. Marker-assisted introgression of the major bacterial blight resistance genes, Xa21 and xa13, and blast resistance gene, Pi54, into the popular rice variety, JGL1798. Mol Breed, 2019,39:12. doi: 10.1007/s11032-018-0919-6
[29]	Kumar N, Shikha D, Kumari S, Choudhary B K, Kumar L, Singh I S. SSR-based DNA Fingerprinting and diversity assessment among Indian germplasm of Euryale ferox: an aquatic underutilized and neglected food crop. Appl Biochem Biotechnol, 2018,185:34-41. doi: 10.1007/s12010-017-2643-9 pmid: 29082475
[30]	Siew G Y, Ng W L, Tan S W, Alitheen N B, Tan S G, Yeap S K. Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers. PeerJ, 2018,6:e4266. doi: 10.7717/peerj.4266 pmid: 29511604
[31]	Tayeh N, Aubert G, Pilet Nayel M L, Lejeune Henaut I, Warkentin T D, Burstin J. Genomic tools in pea breeding programs: Status and perspectives. Front Plant Sci, 2015,6:1037. doi: 10.3389/fpls.2015.01037 pmid: 26640470
[32]	Liu R, Fang L, Yang T, Zhang X Y, Hu J G, Zhang H Y, Han W L, Hua Z K, Hao J J, Zong X X. Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections. Sci Rep, 2017,7:5919. doi: 10.1038/s41598-017-06222-y pmid: 28724947
[33]	Wu X B, Li N N, Hao J J, Hu J G, Zhang X Y, Blair M W. Genetic diversity of Chinese and global pea (Pisum sativum L.) collections. Crop Sci, 2017,57:1-11. doi: 10.2135/cropsci2015.07.0415
[34]	Milczarski P, Bolibok Brągoszewska H, Myśków B, Stojałowski S, Heller Uszyńska K, Góralska M, Brągoszewski P, Uszyński G, Kilian A, Rakoczy Trojanowska M. A high density consensus map of rye (Secale cereale L.) based on DArT markers. PLoS One, 2011,6:e28495. doi: 10.1371/journal.pone.0028495 pmid: 22163026
[35]	Blenda A, Fang D D, Rami J F, Garsmeur O, Luo F, Lacape J M. A high density consensus genetic map of tetraploid cotton that integrates multiple component maps through molecular marker redundancy check. PLoS One, 2012,7:e45739. doi: 10.1371/journal.pone.0045739 pmid: 23029214
[36]	Sudheesh S, Lombardi M, Leonforte A, Cogan N O I, Materne M, Forster J W, Kaur S. Consensus genetic map construction for field pea (Pisum sativum L.), trait dissection of biotic and abiotic stress tolerance and development of a diagnostic marker for the er1 powdery mildew resistance gene. Plant Mol Biol Rep, 2015,33:1391-1403. doi: 10.1007/s11105-014-0837-7
[37]	Sudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan N O I, Materne M, Forster J W, Kaur S. Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.). Mol Breed, 2015,35:185. doi: 10.1007/s11032-015-0376-4
[38]	Yang T, Fang L, Zhang X Y, Hu J G, Bao S Y, Hao J J, Li L, He Y H, Jiang J Y, Wang F, Tian S, Zong X X. High-throughput development of SSR markers from pea (Pisum sativum L.) based on next generation sequencing of a purified Chinese commercial variety. PLoS One, 2015,10:e0139775. doi: 10.1371/journal.pone.0139775 pmid: 26440522
[39]	Kwon S J, Brown A F, Hu J, McGee R, Watt C, Kisha T, Timmerman-Vaughan G, Grusak M, McPhee K E, Coyne C J. Genetic diversity, population structure and genome-wide marker-trait association analysis emphasizing seed nutrients of the USDA pea (Pisum sativum L.) core collection. Genes Genomics, 2012,34:305-320. doi: 10.1007/s13258-011-0213-z
[40]	Kaur S J, Pembleton L W, Cogan N O, Savin K W, Leonforte T, Paull J, Materne M, Forster J W. Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers. BMC Genomics, 2012,13:104. doi: 10.1186/1471-2164-13-104 pmid: 22433453
[41]	Xu S C, Gong Y M, Mao W H, Hu Q Z, Zhang G W, Fu W, Xian Q Q. Development and characterization of 41 novel EST-SSR markers for Pisum sativum (Leguminosae). Am J Bot, 2012,99:E149-E153. doi: 10.3732/ajb.1100445
[42]	Bordat A, Savois V, Nicolas M, Salse J, Chauveau A, Bourgeois M, Potier J, Houtin H, Rond C, Murat F, Marget P, Aubert G, Burstin J. Translational genomics in legumes allowed placing in silico 5460 unigenes on the pea functional map and identified candidate genes in Pisum sativum L. G3: Genes Genom Genet 2011,1:93-103.
[43]	顾竟, 李玲, 宗绪晓, 王海飞, 关建平, 杨涛. 豌豆种质表型性状SSR标记关联分析. 植物遗传资源学报, 2011,12:833-839.
	Gu J, Li L, Zong X X, Wang H F, Guan J P, Yang T. Association analysis between morphological traits of pea and its polymorphic SSR markers. J Plant Genet Resour, 2011,12:833-839 (in Chinese with English abstract).
[44]	Burstin J, Deniot G, Potier J, Weinachter C, Aubert G, Barranger A. Microsatellite polymorphism in Pisum sativum. Plant Breed, 2001,120:311. doi: 10.1046/j.1439-0523.2001.00608.x
[45]	Dellaporta S L, Wood J, Hicks J B. A plant DNA minipreparation: version II. Plant Mol Biol Rep, 1983,1:19-21. doi: 10.1007/BF02712670
[46]	Meng L, Li H H, Zhang L Y, Wang J K. QTL IciMapping: integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J, 2015,3:269-283. doi: 10.1016/j.cj.2015.01.001
[47]	Voorrips R E. MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered, 2002,93:77-78. doi: 10.1093/jhered/93.1.77 pmid: 12011185
[48]	Parida S K, Kalia S K, Kaul S, Dalal V, Hemaprabha G, Selvi A, Pandit A, Singh A, Gaikwad K, Sharma T R, Srivastava P S, Singh N K, Mohapatra T. Informative genomic microsatellite markers for efficient genotyping applications in sugarcane. Theor Appl Genet, 2009,118:327-338. doi: 10.1007/s00122-008-0902-4
[49]	Kamaluddin , Khan M A, Kiran U, Ali A, Abdin M Z, Zargar M Y, Ahmad S, Sofi P A, Gulzar S. Molecular markers and marker-assisted selection in crop plants. In: Abdin M Z, Kiran U, Kamaluddin, Ali A, eds. Plant Biotechnology: Principles and Applications. Singapore: Springer Singapore, 2017. pp 295-328.
[50]	Nadeem M A, Nawaz M A, Shahid M Q, Doğan Y, Comertpay G, Yıldız M, Hatipoğlu R, Ahmad F, Alsaleh A, Labhane N, Özkan H, Chung G, Baloch F S. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnol Biotechnol Equip, 2018,32:261-285. doi: 10.1080/13102818.2017.1400401
[51]	Varshney R K, Graner A, Sorrells M E. Genic microsatellite markers in plants: features and applications. Trends Biotechnol, 2005,23:48-55. doi: 10.1016/j.tibtech.2004.11.005 pmid: 15629858
[52]	Smýkal P, Hybl M, Corander J, Jarkovsky J, Flavell A J, Griga M. Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theor Appl Genet, 2008,117:413-424. doi: 10.1007/s00122-008-0785-4
[53]	宗绪晓, 关建平, 王述民, 刘庆昌. 中国豌豆地方品种SSR标记遗传多样性分析. 作物学报, 2008,34:1330-1338. doi: 10.3724/SP.J.1006.2008.01330
	Zong X X, Guan J P, Wang S M, Liu Q C. Genetic diversity among Chinese pea (Pisum sativum L.) landraces revealed by SSR markers. Acta Agron Sin, 2008,34:1330-1338 (in Chinese with English abstract).
[54]	Zong X X, Ford R, Redden R R, Guan J P, Wang S M. Identification and analysis of genetic diversity structure within Pisum genus based on microsatellite markers. Agric Sci China, 2009,8:257-267. doi: 10.1016/S1671-2927(08)60208-4
[55]	宗绪晓, 关建平, 王述民, 刘庆昌, Redden R R, Ford R. 国外栽培豌豆遗传多样性分析及核心种质构建. 作物学报, 2008,34:1518-1528. doi: 10.3724/SP.J.1006.2008.01518
	Zong X X, Guan J P, Wang S M, Liu Q C, Redden R R, Ford R. Genetic diversity and core collection of alien Pisum sativum L. germplasm. Acta Agron Sin, 2008,34:1518-1528 (in Chinese with English abstract).
[56]	Prakash N, Kumar R, Choudhary V K, Singh C M. Molecular assessment of genetic divergence in pea genotypes using microsatellite markers. Legume Res, 2016,39:183-188.
[57]	Duarte J, Riviere N, Baranger A, Aubert G, Burstin J, Cornet L, Lavaud C, Lejeune Henaut I, Martinant J P, Pichon J P, Pilet Nayel M L, Boutet G. Transcriptome sequencing for high throughput SNP development and genetic mapping in pea. BMC Genom, 2014,15:126. doi: 10.1186/1471-2164-15-126
[58]	Guindon M F, Martin E, Cravero V, Gali K K, Warkentin T D, Cointry E. Linkage map development by GBS, SSR, and SRAP techniques and yield-related QTLs in pea. Mol Breed, 2019,39:54. doi: 10.1007/s11032-019-0949-8
[59]	Aubert G, Morin J, Jacquin F, Loridon K, Quillet M C, Petit A, Rameau C, Lejeune Henaut I, Huguet T, Burstin J. Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula. Theor Appl Genet, 2006,112:1024-1041. doi: 10.1007/s00122-005-0205-y
[60]	Duarte J, Riviere N, Baranger A, Aubert G, Burstin J, Cornet L, Lavaud C, Lejeune Henaut I, Martinant J P, Pichon J P, Pilet Nayel M L, Boutet G. Transcriptome sequencing for high throughput SNP development and genetic mapping in pea. BMC Genom, 2014,15:126. doi: 10.1186/1471-2164-15-126
[61]	Sindhu A, Ramsay L, Sanderson L A, Stonehouse R, Li R, Condie J, Shunmugam A S K, Liu Y, Jha A B, Diapari M, Burstin J, Aubert G, Tar’an B, Bett K E, Warkentin T D, Sharpe A G. Gene-based SNP discovery and genetic mapping in pea. Theor Appl Genet, 2014,127:2225-2241. doi: 10.1007/s00122-014-2375-y
[62]	Sybenga J. Recombination and chiasmata: few but intriguing discrepancies. Genome, 1996,39:473-484. doi: 10.1139/g96-061 pmid: 18469909
[63]	Knox M R, Ellis T H N. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations. Genetics, 2002,162:861-873. pmid: 12399396
[64]	Truong S K, McCormick R F, Morishige D T, Mullet J E. Resolution of genetic map expansion caused by excess heterozygosity in plant recombinant inbred populations. G3: Genes Genom Genet, 2014,4:1963-1969.
[65]	Ellis T H, Turner L, Hellens R P, Lee D, Harker C L, Enard C, Domoney C, Davies D R. Linkage maps in pea. Genetics, 1992,130:649-663. pmid: 1551583

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

连锁群 Linkage group	锚定标记的数目 Number of anchor markers	锚定标记的名称 Name of anchor markers
I	5	AA67, AD147, AF016458, D21, PsAS2
II	3	AA332, AD83, D23
III	4	AA355, AD270, PSAJ3318, PSBLOX13.2
IV	4	AA430942, AA122, AA285, AA315
V	1	PSGAPA1
VI	5	AA335, AB71, AD160, AD60, PSGSR1
VII	3	AB65, AF004843, PSAB60

连锁群 Linkage group	共有标记数目 Number of bridge markers	上图标记数目 Number of mapped markers	图谱长度 Map length (cM)	平均标记密度 Average marker density
I	3	82	976.8	11.9
II	7	68	682.7	10.0
III	8	102	1077.2	10.6
IV	7	104	1058.9	10.2
V	7	125	1018.2	8.1
VI	7	84	801.8	9.5
VII	14	103	977.0	9.5
平均Mean	7.6	95	941.8	10.0
总计Total	53	668	6592.6	69.8

An integrated high-density SSR genetic linkage map from two F₂ population in Chinese pea

RichHTML373

PDF

Abstract

Cite this article

share this article

Figures/Tables 9

References 65

Related Articles 15

Metrics

Comments

Recommended 0

连锁群 Linkage group	上图标记数目 Number of mapped markers		图谱长度 Map length (cM)		平均标记密度 Average marker density
连锁群 Linkage group	PSP1	PSP2	PSP1	PSP2	PSP1	PSP2
I	74	11	751.0	137.4	10.1	12.5
II	62	13	494.9	160.6	8.0	12.4
III	90	20	855.3	158.4	9.5	7.9
IV	97	14	904.7	119.0	9.3	8.5
I-2/V	113	19	822.5	173.3	7.3	9.1
VI	70	21	658.6	175.0	9.4	8.3
VII	97	20	843.6	203.4	8.7	10.2
平均Mean	86	17	761.5	161.0	8.9	9.8
总计Total	603	118	5330.6	1127.1	62.3	68.9

[1]	YU Chun-Miao, ZHANG Yong, WANG Hao-Rang, YANG Xing-Yong, DONG Quan-Zhong, XUE Hong, ZHANG Ming-Ming, LI Wei-Wei, WANG Lei, HU Kai-Feng, GU Yong-Zhe, QIU Li-Juan. Construction of a high density genetic map between cultivated and semi-wild soybeans and identification of QTLs for plant height [J]. Acta Agronomica Sinica, 2022, 48(5): 1091-1102.
[2]	CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[3]	ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[4]	WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[5]	ZHOU Xin-Tong, GUO Qing-Qing, CHEN Xue, LI Jia-Na, WANG Rui. Construction of a high-density genetic map using genotyping by sequencing (GBS) for quantitative trait loci (QTL) analysis of pink petal trait in Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(4): 587-598.
[6]	HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[7]	LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[8]	GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[9]	WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[10]	Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[11]	Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[12]	HU Wen-Jing,ZHANG Yong,LU Cheng-Bin,WANG Feng-Ju,LIU Jin-Dong,JIANG Zheng-Ning,WANG Jin-Ping,ZHU Zhan-Wang,XU Xiao-Ting,HAO Yuan-Feng,HE Zhong-Hu,GAO De-Rong. Mapping and genetic analysis of QTLs for Fusarium head blight resistance to disease spread in Yangmai 16 [J]. Acta Agronomica Sinica, 2020, 46(02): 157-165.
[13]	YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[14]	Zhi-Jun TONG,Yi-Han ZHANG,Xue-Jun CHEN,Jian-Min ZENG,Dun-Huang FANG,Bing-Guang XIAO. Mapping of quantitative trait loci conferring resistance to brown spot in cigar tobacco cultivar Beinhart1000-1 [J]. Acta Agronomica Sinica, 2019, 45(3): 477-482.
[15]	CHEN Fang,QIAO Lin-Yi,LI Rui,LIU Cheng,LI Xin,GUO Hui-Juan,ZHANG Shu-Wei,CHANG Li-Fang,LI Dong-Fang,YAN Xiao-Tao,REN Yong-Kang,ZHANG Xiao-Jun,CHANG Zhi-Jian. Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357 [J]. Acta Agronomica Sinica, 2019, 45(10): 1503-1510.

An integrated high-density SSR genetic linkage map from two F2 population in Chinese pea

RichHTML373

PDF

Abstract

Cite this article

share this article

Figures/Tables 9

References 65

Related Articles 15

Metrics

Comments

Recommended 0

An integrated high-density SSR genetic linkage map from two F₂ population in Chinese pea