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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (1): 19-29.doi: 10.3724/SP.J.1006.2021.01050

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Overexpression of TaJRL53 enhances the Fusarium head blight resistance in wheat

CHEN Tong-Rui(), LUO Yan-Jun, ZHAO Pan-Ting, JIA Hai-Yan*(), MA Zheng-Qiang   

  1. College of Agriculture, Nanjing Agricultural University / State Key Laboratory for Crop Genetics and Germplasm Enhancement / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, Jiangsu, China
  • Received:2020-06-17 Accepted:2020-09-13 Online:2021-01-12 Published:2020-09-30
  • Contact: JIA Hai-Yan E-mail:2017101089@njau.edu.cn;hyjia@njau.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2016YFD0101004)

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Abstract:

Jacalin-related lectins (JRLs) are a group of plant lectins with jacalin protein domain, which play important roles during plant response to biotic and abiotic stress. According their carbohydrate binding specificities, the JRLs were described as two types: galactose-binding acalins (gJRLs) and mannose-binding jacalins (mJRLs). Previous studies have shown that TaJRL53 encodes a protein with Jacalin and Dirigent domains and can be up-regulated by Fusarium graminearum infection. Wheat FHB induced by this pathogen is a disastrous disease, which not only reduces the grain yield, but also contains deoxynivalenol and other toxins, which seriously affect the health of human and livestock. To dissect the gene’s function in FHB resistance, we silenced and overexpressed the TaJRL53 in wheat respectively. As a result, attenuating TaJRL53 increased susceptibility to Fusarium graminearum. And susceptible wheat transformed with TaJRL53 over-expression showed higher Fusarium head blight resistance, the number of diseased spikelets (NDS) and length of diseased spike rachis (LDR) of transgenics wheat with TaJRL53 overexpression were significantly reduced than those of wild type. After Fusarium graminearum infection, the expression of ROS synthesis pathway, JA synthesis and signaling pathways marker genes, and disease related genes in TaJRL53 over-expression transgenic plants were higher than those in non-transgenic control. These results suggest that TaJRL53 enhanced the FHB resistance in wheat through regulating ROS synthesis pathway and JA signal transduction pathways. This study enhanced the understanding of wheat JRLs family genes’ function and laid a foundation for the mechanism dissection of TaJRL53 resistant to FHB in wheat.

Key words: wheat, Jacalin-related lectins, FHB resistance, ROS, JA pathway

Fig. 1

Scheme of the TaJRL53 overexpression vector"

Fig. 2

Tissue-specific expression of TaJRL53 A: seedling stage; B: flowering stage. Bar=50 μm."

Fig. 3

Expression of TaJRL53-GFP fusion protein in onion epidermal cells"

Fig. 4

The FHB resistance of spikes after TaJRL53 silenced A: Expression level of TaJRL53 in spike after inoculation with virus; B: Number of disease spikelet; C: Length of diseased rachides; D: Disease symptoms of spikes; The numbers in brackets show the number of samples. * and *** represent significant difference at level P < 0.05 and P < 0.001 (Student’s t-test)."

Fig. 5

PCR test and expression level of TaJRL53 in T0 transgenic plants with TaJRL53 overexpression M: marker; 1-6: transgenic plants from Bobwhite; 7: transgenic plant from PH691. PH: PH691; Bob: Bobwhite; H: H2O; P: positive control."

Fig. 6

FHB resistance of TaJRL53 overexpression transgenic plants A: inoculated leaf; B: lesion area; C: inoculated spike; D: number of diseases spikelets; E: length of diseased rachide. 1-5: transgenic plants from Bobwhite; 6: transgenic plant from Ph691. The numbers in brackets of B, D, and E show the number of samples. *, **, and ***: significant difference at P < 0.05, P < 0.01, and P < 0.001 (Student’s t-test)."

Fig. 7

Expression of marker genes in resistance signaling pathways A-C: marker genes in JA singnal pathway; D, H, I: pathogenesis-related genes; E, F: ROS biosynthesis-related genes; G: ROS transport-related genes; J-L: SA singnal pathway-related genes; M, N: SA biosynthesis-related genes; O: lignin biosynthesis genes; Bob: Bobwhite; 1-4: transgenic plants from Bobwhite; * and **: significant difference at P < 0.05 and P < 0.01 (Student’s t-test)."

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