Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (2): 511-517.doi: 10.3724/SP.J.1006.2022.11025

• RESEARCH NOTES • Previous Articles     Next Articles

Molecular cytogenetic identification of wheat-Thinopyrum intermedium 2A/6St substitution strain 014-459

TAO Jun1,2(), LAN Xiu-Jin1,*()   

  1. 1Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
    2Mianyang Academy of Agricultural Sciences, Mianyang 621023, Sichuan, China
  • Received:2021-03-09 Accepted:2021-06-16 Online:2022-02-12 Published:2021-06-29
  • Contact: LAN Xiu-Jin E-mail:tj4bmy@163.com;lanxiujin@163.com

RichHTML394

10

PDF

216

Abstract:

Thinopyrum intermedium is a valuable resource for wheat genetic improvement, and numerous wheat-Th. intermedium chromosome addition and substitution lines and partial amphiploids have been developed. Zhong 4 is a wheat-Th. intermedium partial amphidiploid, which can easily hybridize with wheat and is extensively utilized for wheat improvement. The strain 014-459 was a progeny of partial amphidiploid Zhong 4 descendant of wheat and Th. intermedium with some special characteristics such as high crude protein and wet gluten content and partial F1 of common wheat cultivars and strain 014-459 were sterility while a few others were fertility despite reciprocal cross. Molecular cytogenetic identification of strain 014-459 were detected for speculated contained fragment of Th. intermedium chromosomes based on its special characters. Genomic composition of strain 014-459 was detected by FISH, GISH, and PLUG (polymerase chain reaction-based landmark unique gene) marker analysis. Combined FISH/GISH analysis confirmed that a pair of wheat 2A chromosomes in strain 014-459 of the hybrid progeny between wheat-Thinopyrum intermedium partial amphidiploid Zhong 4 and wheat was replaced by a pair of St chromosomes from Thinopyrum intermedium. PLUG marker analysis verified that this pair belonged to the sixth homologous group. The special characters of strain 014-459 might be attributable to the substitution of Th. intermedium St chromosome into wheat. The study of molecular cytogenetic identification of strain 014-459 was probably beneficial to quality improvement and utilization of 6St chromosome of Th. intermedium in wheat breeding.

Key words: GISH, FISH, substitution strain, Pseudoroegneria strigosa, cytogenetics

Fig. 1

Meiosis in wheat-Thinopyrum intermedium A-B: meiotic metaphase I; C-E: meiotic anaphase I; F-H: meiotic telophase I."

Fig. 2

In situ hybridization and pollen mother-cell meiosis in wheat-Thinopyrum intermedium A: FISH map of strain 014-459. Oligo-pSc119.2-2 (green), oligo-pTa535-2 (red); B: GISH map of strain 014-459. DNA of Th. intermedium used as the probe, and DNA of CS was used as the blocker; C: GISH map of strain 014-459. DNA of Pseudoroegneria strigosa was used as the probe and DNA of CS was used as the blocker; D: FISH map of strain 014-459. St2-80 was used as the probe and DNA of CS was used as the blocker; E: GISH map of pollen mother cells of strain 014-459 at the meiotic metaphase. DNA of Th. intermedium was used as the probe and DNA of CS was used as the blocker."

Fig. 3

In situ hybridization in wheat-Thinopyrum intermedium A: FISH map of strain 014-459. Oligo-pSc119.2-2 (green), oligo-pTa535-2 (red); B: GISH map of strain 014-459. DNA of Th. intermedium used as the probe, and DNA of CS was used as the blocker; C: GISH map of strain 014-459. DNA of rye was used as the probe and DNA of Chinese Spring (CS) wheat was used as the blocker."

Fig. 4

PLUG marker analysis in wheat-Thinopyrum intermedium A: TNAC1763 amplification product (Taq I digestion), with arrows indicating St-specific bands; B: TNAC1178 amplification product (Taq I digestion), with arrows indicating a CS-specific amplification band, which was absent from 014-459 and St (Pseudoroegneria strigosa)."

[1] Salina E A, Adonina I G, Badaeva E D, Kroupin P Y, Stasyuk A I, Leonova I N, Shishkina A A, Divashuk M G, Starikova E V, Khuat T M L. A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases. Euphytica, 2015,204:91-101.
[2] Chen Q, Conner R L, Ahmad F, Laroche A, Fedak G, Thomas J B. Molecular characterization of the genome composition of partial amphiploids derived from Triticum aestivum×Thinopyrum ponticum and T. aestivum×T. intermedium as sources of resistance to wheat streak mosaic virus and its vector, Aceria tosichella. Theor Appl Genet, 1998,97:1-8.
[3] Bao Y, Li X, Liu S, Cui F, Wang H. Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium partial amphiploid resistant to powdery mildew and stripe rust. Cytogenet Genome Res, 2009,126:390-395.
[4] 祁适雨, 于世选, 张耀辉, 于光华, 宋凤英. 春小麦与天蓝冰草远缘杂交育种的研究. 中国农业科学, 1979,12(2):1-11.
Qi S Y, Yu S S, Zhang Y H, Yu G H, Song F Y. Studies on wheat breeding by distant hybridization between wheat and Agropyron glaucum. Sci Agric Sin, 1979,12(2):1-11 (in Chinese with English abstract).
[5] Li H, Wang X. Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat. J Genet Genom, 2009,36:557-565.
[6] Li G, Wang H, Lang T, Li J, La S, Yang E, Yang Z. New molecular markers and cytogenetic probes enable chromosome identification of wheat- Thinopyrum intermedium introgression lines for improving protein and gluten contents. Planta, 2016,244:1-12.
[7] Georgieva M, Sepsi A, Tyankova N, Molnár-Láng M. Molecularcytogeneticcharacterization of two high protein wheat-Thinopyrum intermedium partialamphiploids. J Appl Genet, 2011,52:269-277.
[8] 祁适雨, 肖志敏, 辛文利, 孙连发, 张延滨, 何孟元, 卜秀玲, 韩方普, 辛志勇, 马有志. “远中”号小偃麦在小麦育种中的应用. 麦类作物学报, 2000,20:10-15.
Qi S Y, Xiao Z M, Xin W L, Sun L F, Zhang Y B, He M Y, Pu X L, Han F P, Xin Z Y, Ma Y Z. Utilization of Yuanzhong octopioidy Trititrigia in wheat breeding. J Triticeae Crops, 2000,20:10-15 (in Chinese with English abstract).
[9] 薛秀庄, 王祥正. 八倍体小偃麦中3、中4、中5在小麦育种中的应用. 陕西农业科学, 1989, (5):1-5.
Xue X Z, Wang X Z. Utilization of octoploid tritrigia Zhong 3, Zhong 4 and Zhong 5. Shaanxi J Agric Sci, 1989, ( 5):1-5 (in Chinese with English abstract).
[10] 王黎明, 林小虎, 张平杰, 张志雯, 王玉海, 赵逢涛, 高居荣, 李文才, 李兴峰, 王洪刚. 小麦-中间偃麦草二体异代换系山农0095的选育及其鉴定. 中国农业科学, 2005,38:1958-1964.
Wang L M, Lin X H, Zhang P J, Zhang Z W, Wang Y H, Zhao F T, Gao J R, Li W C, Li X F, Wang H G. Breeding and characterization of wheat-Thinopyrum intermedium alien substitution line Shannong 0095. Sci Agric Sin, 2005,38:1958-1964 (in Chinese with English abstract).
[11] 张耀辉, 宋建荣, 岳维云, 吕莉莉, 王希恩. 八倍体小偃麦中5的利用. 甘肃农业科技, 2007, ( 10):8-10.
Zhang Y H, Song J R, Yue W Y, Lyu L L, Wang X E. The use of octoploid tritelytrigia variety, Zhong 5. Gansu Agric Sci Technol, 2007, ( 10):8-10 (in Chinese).
[12] 杨永乾, 李小军, 宋杰, 孙玉, 茹振钢. 小麦-中间偃麦草代换系中233的分子细胞学鉴定. 麦类作物学报, 2014,34:449-453.
Yang Y Q, Li X J, Song J, Sun Y, Ru Z G. Molecular and cytological identification of wheat-Thinopyrum intermedium alien substitution line Zhong 233. J Triticeae Crops, 2014,34:449-453 (in Chinese with English abstract).
[13] 李爱博, 李金荷, 宋建荣, 王化俊, 张耀辉, 尚勋武, 曹世勤, 李葆春. 小麦-中间偃麦草抗锈易位系中梁27的鉴定及分析. 麦类作物学报, 2015,35(11):27-31.
Li A B, Li J H, Song J R, Wang H J, Zhang Y H, Shang X W, Cao S Q, Li B C. Idientification and analysis of Zhongliang 27, a wheat-Thinopyrum intermedium translocation line with resistance to stripe rust. J Triticeae Crops, 2015,35(11):27-31 (in Chinese with English abstract).
[14] Stebbins G L, Pun F T. Artificial and natural hybrids in the Gramineae, tribe hordeae: VI. Chromosome pairing in Secale cereale × Agropyron intermedium and the problem of genome homologies in the Triticinae. Genetics, 1953,38:600-608.
[15] Dewey D R. The genome structure of intermediate wheatgrass. J Hered, 1962,53:282-290.
[16] Xu J, Conner R L. Intravarietal variation in satellites and C-banded chromosomes of Agropyron intermedium ssp. Trichophorum cv. Greenleaf. Genome, 1994,37:305-310.
[17] Liu Z W, Wang R R. Genome analysis of Elytrigia caespitosa, Lophopyrum nodosum, Pseudoroegneria geniculata ssp. scythica and Thinopyrum intermedium (Triticeae: Gramineae). Genome, 1993,36:102-111.
[18] Assadi M, Runemark H. Hybridisation, genomic constitution and generic delimitation in Elymus s. l.(Poaceae: Triticeae). Plant Syst Evol, 1995,194:189-205.
[19] Le H T, Armstrong K C, Miki B. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Mol Biol Rep, 1990,7:150-158.
[20] Heslopharrison J S, Leitch A R, Schwarzacher T, Anamthawatjónsson K. Detection and characterization of 1B/1R translocations in hexaploid wheat. Heredity, 1990,65:385-392.
[21] Schwarzacher T, Anamthawat-Jónsson K, Harrison G E, Islam A K M R, Jia J Z, King I P, Leitch A R, Miller T E, Reader S M, Rogers W J. Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theor Appl Genet, 1992,84:778-786.
[22] 吉万全, 薛秀庄, 王秋英, 王长有, Fedak G, Patroski R, 刘广田. 中间偃麦草的GISH分析. 西北植物学报, 2001,21:401-405.
Ji W Q, Xue X Z, Wang Q Y, Wang C Y, Fedak G, Patroski R, Liu G T. GISH analysis of Thinopyrum intermedium. Acta Bot Boreal-Occident Sin, 2001,21:401-405 (in Chinese with English abstract).
[23] Zhang X Y, Koul A, Petroski R, Ouellet T, Fedak G, Dong Y S, Nwang R R. Molecular verification and characterization of BYDV-resistant germ plasms derived from hybrids of wheat with Thinopyrum ponticum and Th intermedium. Theor Appl Genet, 1996,93:1033-1039.
[24] Chen Q, Conner R L, Laroche A, Thomas J B. Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization. Genome, 1998,41:580-586.
[25] Chen Q. Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe: a landmark approach for Thinopyrum genome research. Cytogenet Genome Res, 2005,109:350-359.
[26] Chen Q, Friebe B, Conner R L, Laroche A, Thomas J B, Gill B S. Molecular cytogenetic characterization of Thinopyrum intermedium-derived wheat germplasm specifying resistance to wheat streak mosaic virus. Theor Appl Genet, 1998,96:1-7.
[27] Tang S, Li Z, Jia X, Larkin P J. Genomic in situ hybridization (GISH) analyses of Thinopyrum intermedium, its partial amphiploid Zhong 5, and disease-resistant derivatives in wheat. Theor Appl Genet, 2000,100:344-352.
[28] 孙善澄. 小偃麦新品种与中间类型的选育途径、程序和方法. 作物学报, 1981,7:51-58.
Sun S C. The approach and methods of breeding new varieties and new species from Agrotriticum hybrids. Acta Agron Sin, 1981,7:51-58 (in Chinese with English abstract).
[29] Liu Z, Li D Y, Zhang X Y. Genetic relationships among five basic genomes St, E, A, band D in Triticeae revealed by genomic southern and insitu hybridization. Integr Plant Biol, 2007,49:1080-1086.
[30] Han F P, Liu B, Fedak G, Liu Z H. Genomic constitution and variation in five partial amphiploids of wheat- Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor Appl Genet, 2004,109:1070-1076.
[31] Rayburn A L, Gill B S. Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol Biol Rep, 1986,4:102-109.
[32] Rayburn A L, Gill B S. Molecular analysis of D-genome of the Triticea. Theor Appl Genet, 1987,73:385-388.
[33] Mcintyre C L, Pereira S, Moran L B, Appels R. New Secale cereale(rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome, 1990,33:635-640.
[34] Mukai Y, Nakahara Y, Yamamoto M. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome, 1993,36:489-494.
[35] Pedersen C, Langridge P. Identification of the entire chromosome complement of bread wheat by two-colour FISH. Genome, 1997,40:589-593.
[36] Tang Z, Yang Z, Fu S. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet, 2014,55:313-318.
[37] Ishikawa G, Nakamura T T, Saito M, Nasuda S, Endo T R, Wu J, Matsumoto T. Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers. Theor Appl Genet, 2009,118:499-514.
[38] Hu L J, Li G R, Zeng Z X, Chang Z J, Cheng L, Zhou J P, Yang Z J. Molecular cytogenetic identification of a new wheat- Thinopyrum substitution line with stripe rust resistance. Euphytica, 2011,177:169-177.
[39] 陶军, 兰秀锦. 对具有一个共同亲本的杂种F1育性差异的初步研究. 四川农业大学学报, 2020,38(1):1-7.
Tao J, Lan X J. Preliminary study on fertility difference of F1 hybrids based on a common parent. J Sichuan Agric Univ, 2020,38(1):1-7 (in Chinese with English abstract).
[40] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001,103:455-461.
[41] Komuro S, Endo R, Shikata K, Kato A. Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome, 2013; 56:131-137.
[42] Zeng D Y, Luo J T, Li Z L, Chen G, Zhang L Q, Ning S Z, Yuan Z W, Zheng Y L, Hao M, Liu D C. High transferability of homoeolog-specific markers between bread wheat and newly synthesized hexaploid wheat lines. PLoS One, 2016,11:e0162847.
[43] Hao M, Luo J T, Yang M, Zhang L Q, Yan Z H, Yuan Z W, Zheng Y L, Zhang H G, Liu D C. Comparison of homoeologous chromosome pairing between hybrids of wheat genotypes Chinese Spring ph1b and Kaixian-luohanmai with rye. Genome, 2011,54:959-964.
[44] Wang L, Shi Q H, Su H D, Wang Y, Sha L N, Fan X, Kang H Y, Zhang H Q, Zhou Y H. St2-80: a new FISH marker for St genome and genome analysis in Triticeae. Genome, 2017,60:553-563.
[45] Wan Y, Liu K, Wang D, Shewry P R. High-molecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat. Theor Appl Genet, 2000,101:879-884.
[46] 茹岩岩, 张学勇, 李大勇, 游光霞, 晏月明. 对基因组原位杂交信号释译可能出现的片面性——来自一个小麦易位系(A-3)中外源遗传物质鉴定的启示. 作物学报, 2002,28:6-10.
Ru Y Y, Zhang X Y, Li D Y, You G X, Yan Y M. Risk in explanation of GISH signals enlightenment from verification of alien chromatin in a wheat translocation line (A3) by GISH. Acta Agr Sin, 2002,28:6-10 (in Chinese with English abstract).
[47] Linc G, Gaál E, Molnár I, Icsó D, Molnár-Láng M. Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship. PLoS One, 2017,12:e0173623.
[48] Martín P, Gómez M, Carrillo J M. Interaction between allelic variation at the Glu-D1 locus and a 1BL.1RS translocation on flour quality in bread wheat. Crop Sci, 2001,41:1080-1084.
[49] Clarke B C, Mukai Y, Appels R. The Sec-1 locus on the short arm of chromosome 1R of rye(Secale cereale). Chromosoma, 1996,105:269-275.
[50] Burnett C J, Lorenz K J, Carver B F. Effects of the 1B/1R translocation in wheat on composition and properties of grain and flour. Euphytica, 1995,86:159-166.
[51] Graybosch R A, Peterson C J, Shelton D R, Baenziger P S. Genotypic and environmental modification of wheat flour protein composition in relation to end-use quality. Crop Sci, 1996,36:296-300.
[52] Li Z, Ren T H, Yan B J, Tan F Q, Yang M Y, Ren Z L. A mutant with expression deletion of gene sec-1 in a 1RS.1BL line and its effect on production quality of wheat. PLoS One, 2016,11:e0146943.
[1] Lin-Sheng WANG,Ya-Li ZHANG,Guang-Hui NAN. Molecular and Cytogenetic Identification of Triticum aestivum-Leymus racemosus Translocation Line T5AS-7LrL·7LrS [J]. Acta Agronomica Sinica, 2018, 44(10): 1442-1447.
[2] QI Xiao-Lei,BAO Yin-Guang,LI Xing-Feng,QIAN Zhao-Guo,WANG Rui-Xia,WU Ke,WANG Hong-Gang. Cytological Identification and Chromosome Constitution Analyses of Ten Octoploid Trititrigia Accessions [J]. Acta Agron Sin, 2017, 43(07): 967-973.
[3] LI Li-Na,,DU Pei,FU Liu-Yang,LIU Hua,XU Jing,QIN Li,YAN Mei,HAN Suo-Yi,HUANG Bing-Yan,DONG Wen-Zhao,TANG Feng-Shou,ZHANG Xin-You. Development and Characterization of Amphidiploid Derived from Interspecific Cross between Cultivated Peanut and Its Wild Relative Arachis oteroi [J]. Acta Agron Sin, 2017, 43(01): 133-140.
[4] WANG Xiu-Juan,CHEN Xin-Hong,PANG Yu-Hui,JING Fan,ZHANG Jun,HU Si-Yuan,ZAN Kai,WU Jun,YANG Qun-Hui,ZHAO Ji-Xin *. Molecular Cytogenetic Characterization of Triticum–Psathyrostachys Substitution Line DH2322 [J]. Acta Agron Sin, 2015, 41(02): 207-213.
[5] LI Jian-Bo,QIAO Lin-Yi,LI Xin,ZHANG Xiao-Jun,ZHAN Hai-Xian,GUO Hui-Juan,REN Yong-Kang,CHANG Zhi-Jian. Molecular Mapping of Powdery Mildew Resistance Gene PmCH7124 in a Putative Wheat–Thinopyrum intermedium Introgression Line [J]. Acta Agron Sin, 2015, 41(01): 49-56.
[6] LUO Qiao-Ling,ZHENG Qi,XU Yun-Feng,LI Li-Hui,HAN Fang-Pu,XU Hong-Xing,LI Bin,MA Peng-Tao,AN Diao-Guo. Main Agronomic Traits of 390 Wheat-Rye Derivatives and GISH/FISH Identification of Their Outstanding Materials [J]. Acta Agron Sin, 2014, 40(08): 1331-1339.
[7] SUN Cui-Hua,HOU Li-Yuan,GUO Hui-Juan,ZHANG Xiao-Jun,JIA Ju-Qing,LI Xin,ZHAN Hai-Xian,CHANG Zhi-Jian. Molecular Mapping of Powdery Mildew Resistance Gene pmCH83 in a Putative Wheat–Thinopyrum intermedium Cryptic Introgression Line [J]. Acta Agron Sin, 2013, 39(12): 2107-2114.
[8] CUI Cheng-Qi,WANG Lin-Sheng,CHEN Pei-Du. Molecular and Cytogenetic Identification of Triticum aestivum–Leymus racemosus Translocation Lines T7BS•7Lr#1S and T2AS•2AL-7Lr#1S [J]. Acta Agron Sin, 2013, 39(02): 191-197.
[9] ZHANG Jia-Jia,YUAN Hong-Xia,ZHANG Rui-Qi,XING Xiao-Ping,DAI Jun-Li,NIU Ji-Shan,LI Hong-Lian,CHEN Pei-Du. Analysis of Resistance to Heterodera filipjevi in Triticum aestivum–Dasypyrum villosum Germplasm [J]. Acta Agron Sin, 2012, 38(11): 1969-1976.
[10] ZHAO Hai-Bin,ZHANG Yan-Ming,SHI Chun-Long,YAN Xiao-Dan,TIAN Chao,LI Yong-Peng,LI Ji-Lin. Development and Cytogenetic Analysis of Perennial Wheat in Cold Region [J]. Acta Agron Sin, 2012, 38(08): 1378-1386.
[11] LIU Jin-Shuai, LAI Hui-Cheng, JIA Zhen-Hong. Image Segmentation of Cotton Based on YCbCcr Color Space and Fisher Discrimination Analysis [J]. Acta Agron Sin, 2011, 37(07): 1274-1279.
[12] SONG Zhao-Jian,DU Chao-Qun,HU E-Ping,TANG Zhi-Qiang,CHEN Dong-Ling,HE Yu-Chi. Identification and Comparison of diploid and Tetraploid Hybrids of Oryza sativa X O.meyeriana [J]. Acta Agron Sin, 2010, 36(07): 1144-1152.
[13] LI Fang, ZHANG Gai-Sheng.
FISH Analysis of Resynthesized Allotetraploid Arabidopsis
[J]. Acta Agron Sin, 2010, 36(07): 1216-1220.
[14] PENG Ren-Hai;SONG Guo-Li;LIU Fang;LI Shao-Hui;WANG Chun-Ying;ZHANG Xiang-Di;WANG Yu-Hong;WANG Kun-Bo. An Efficient Method of Cotton DNA Fibers Preparation for FISH [J]. Acta Agron Sin, 2009, 35(3): 412-417.
[15] KONG Fang;JIANG Jin-Jin;WU Lei;WANG You-Ping. Relationships among Genome A, B, and C Revealed by FISH and CAPS [J]. Acta Agron Sin, 2008, 34(07): 1188-1192.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd