铝胁迫下甘蓝型油菜苗期光合相关性状的QTL定位及候选基因筛选

doi:10.3724/SP.J.1006.2022.14225

Abstract

Abstract:

Due to excessive soil acidification, aluminum in soil has become one of the main factors limiting crop growth and yield. In this study, a high generation of recombinant inbred line (RIL) population constructed from Brassica napus L. varieties Zhongshuang 11 (ZS11) and 10D130 treated with 2400 µmol L^-1 aluminum solution was used as experimental materials. To explore the photosynthetic capacity and chlorophyll fluorescence related traits of rapeseed seedling stage under aluminum toxicity stress, we calculated their relative values (treatment/control) and mapped their QTLs, and screened suitable candidate genes according to the confidence interval of QTLs. The results showed that there was significantly correlation between photosynthetic capacity and chlorophyll fluorescence parameters, but there was no significant correlation between photosynthetic capacity and the chlorophyll fluorescence parameters. Relative P_n, relative G_s, relative C_i, relative Φ_PSII, relative F_v/F_o, relative F_v′/F_m′, relative q_P, relative ETR, relative NPQ, and relative dry weight were investigated and QTLs mapping were analyzed. A total of 16 QTLs related to aluminum stress and photosynthesis were detected. The locus was located on chromosomes A03, A05, A06, A08, A09, A10, C02, and C09, respectively. LOD value was 2.05-3.18, and the phenotypic variation explained was 8.1%-12.3%. The confidence intervals of QTLs for relative G_s and relative F_v'/F_m', relative Φ_PSII and relative ETR were found completely overlap at 106.038-109.129 cM of chromosome A03, 145.355-167.417 cM of chromosome A06, respectively. 47 candidate genes that may be related to aluminum stress and photosynthesis were screened, involving organic acid secretion, metal ion transport, hormone regulation, initiation of antioxidant protection, chloroplast self-regulation, and phosphorus uptake regulation. This study lays the foundation for the function research of aluminum resistance and related photosynthetic genes and the breeding of aluminum tolerant and high photosynthetic efficiency rapeseed.

Key words: aluminum stress, photosynthetic traits, QTLs, candidate genes screening, Brassica napus L.

WU Jia-Yi, YUAN Fang, MENG Li-Jiao, LI Chen-Yang, SHI Hong-Song, BAI Yan-Song, WU Xiao-Ru, LI Jia-Na, ZHOU Qing-Yuan, CUI Cui. QTL mapping and candidate genes screening of photosynthesis-related traits in Brassica napus L. during seedling stage under aluminum stress[J].Acta Agronomica Sinica, 2022, 48(11): 2749-2764.

Figures/Tables 7

Fig. 1

Table 1

Fig. 2

Table 2

Table 3

Table 4

Candidate genes related to Aluminium stress and photosynthesis"

性状 Trait	物理区间 Physical interval	甘蓝型油菜基因编号 Gene ID in B. napus	拟南芥基因登录号 Gene accession	基因注释 Gene annotation
RPn	1006640-1008278	BnaA10g01990D	AT1G03550	分泌载体膜家族蛋白 Secretory carrier membrane protein (SCAMP) family protein
	1017170-1017425	BnaA10g02020D	AT1G03600	PSB27
	1159799-1160267	BnaA10g02210D	AT1G03850	谷氧还家族蛋白 Glutaredoxin family protein
	1416448-1417208	BnaA10g02700D	AT1G04560	AWPM-19家族蛋白 AWPM-19-like family protein
	1420412-1425818	BnaA10g02720D	AT1G04580	醛氧化酶 4 Aldehyde oxidase 4 (AO4)
	270316-271150	BnaA10g00540D	AT1G01360	脱落酸受体调节成分1 Regulatory component of ABA receptor 1 (RCAR1)
	2297229-2298311	BnaA10g04360D	AT1G06680	光系统II亚基 P-1 Photosystem II subunit P-1
	2428863-2433922	BnaA10g04550D	AT1G07110	果糖-2,6-二磷酸酯酶 Fructose-2,6-bisphosphatase (F2KP)
	4967798-4968762	BnaA10g06520D	AT5G53160	脱落酸受体调节成分3 Regulatory components of ABA receptor 3 (RCAR3)
	5777349-5778320	BnaA10g07350D	AT5G54270	捕光色素叶绿素a/b结合蛋白 3 Light-harvesting chlorophyll a/b-binding protein 3 (LHCB3)
RGs	11450896-11455054	BnaA03g23880D	AT4G09020	异淀粉酶3 Isoamylase 3 (ISA3)
	11592964-11594227	BnaA03g24110D	AT4G09670	氧化还原酶家族蛋白 Oxidoreductase family protein
	11703183-11704457	BnaA03g24350D	AT4G10340	捕光色素叶绿素a/b结合蛋白5 Light harvesting chloroplyll a/b-binding protein 5 (LHCB5)
性状 Trait	物理区间 Physical interval	甘蓝型油菜基因编号 Gene ID in B. napus	拟南芥基因登录号 Gene accession	基因注释 Gene annotation
	12051498-12054303	BnaA03g24930D	AT4G12020	WRKY19
RCi	1050779-1052616	BnaC02g02350D	AT5G05340	过氧化物酶超家族蛋白 Peroxidase superfamily protein
	1312700-1315286	BnaC02g02870D	AT5G03860	苹果酸合酶 Malate synthase (MLS)
	1338228-1339653	BnaC02g02940D	AT2G18960	H(+)-ATP酶 H(+)-ATPase 1 (HA1)
RΦPS	23332213-23333764	BnaA06g35540D	AT5G47180	植物 VAMP (囊泡相关膜蛋白)家族蛋白 Plant VAMP (vesicle-associated membrane protein) family protein
	23354413-23359357	BnaA06g35590D	AT5G47110	叶绿素a/b结合家族蛋白 Chlorophyll a/b binding family protein
	24314147-24316076	BnaA06g37230D	AT4G38970	果糖二磷酸醛缩酶2 Fructose-bisphosphate aldolase 2 (FBA2)
	24206087-24209026	BnaA06g37120D	AT5G42480	叶绿体的积累和复制6 Accumulation and replication of chloroplasts 6 (ARC6)
Rfv	44394910-44396026	BnaC09g43010D	AT3G58810	金属耐受蛋白A2 Metal tolerance protein A2 (MTPA2)
	44536233-44537627	BnaC09g43140D	AT5G14040	磷酸盐转运蛋白3 Phosphate transporter 3
	46193139-46193697	BnaC09g46220D	AT3G21490	重金属转运/解毒超家族蛋白 Heavy metal transport/detoxification superfamily protein
	48190280-48191717	BnaC09g50250D	AT5G05000	叶绿体外膜蛋白转运机器的构件蛋白34 Translocon at the outer envelope membrane of chloroplasts 34 (TOC34)
Rfv°	13146862-13148728	BnaA03g26740D	AT4G00910	铝活化的苹果酸盐转运家族蛋白 Aluminium activated malate transporter family protein
	4120082-4121722	BnaA09g08390D	AT3G32980	过氧化物酶超家族蛋白 Peroxidase superfamily protein
	4519220-4519695	BnaA09g09080D	AT2G34430	捕光叶绿素蛋白复合物II亚基 Light-harvesting chlorophyll-protein complex II subunit B1 (LHB1B1)
RqP	23778823-23781902	BnaA03g46370D	AT4G23980	生长素应答因子9 Auxin response factor 9 (ARF9)
	27082166-27084124	BnaA03g52000D	AT4G32320	抗坏血酸过氧化物酶6 Ascorbate peroxidase 6 (APX6)
	27439846-27442123	BnaA03g52600D	AT5G51470	生长素应答GH3家族蛋白 Auxin-responsive GH3 family protein
	27829209-27831558	BnaA03g53180D	AT4G35090	过氧化氢酶2 Catalase 2 (CAT2)
RETR	23332213-23333764	BnaA06g35540D	AT5G47180	植物 VAMP (囊泡相关膜蛋白)家族蛋白 Plant VAMP (vesicle-associated membrane protein) family protein
	23354413-23359357	BnaA06g35590D	AT5G47110	叶绿素a/b结合家族蛋白 Chlorophyll a/b binding family protein
	24206087-24209026	BnaA06g37120D	AT5G42480	叶绿体的积累和复制 6 Accumulation and replication of chloroplasts 6 (ARC6)
	24314147-24316076	BnaA06g37230D	AT4G38970	果糖二磷酸醛缩酶2 Fructose-bisphosphate aldolase 2 (FBA2)
性状 Trait	物理区间 Physical interval	甘蓝型油菜基因编号 Gene ID in B. napus	拟南芥基因登录号 Gene accession	基因注释 Gene annotation
	11764739-11766413	BnaA08g13620D	AT4G28610	磷饥饿响应1 Phosphate starvation response 1 (PHR1)
RNPQ	525671-526016	BnaA09g00850D	AT2G02930	谷胱甘肽S-转移酶 F3 Glutathione S-transferase F3 (GSTF3)
	530729-531954	BnaA09g00880D	AT4G02530	叶绿体类囊体腔蛋白 Chloroplast thylakoid lumen protein
	607437-608064	BnaA09g01080D	AT4G02770	光系统I亚基D-1 Photosystem I subunit D-1 (PSAD-1)
	392076-395981	BnaA09g00710D	AT4G02280	蔗糖合酶3 Sucrose synthase 3 (SUS3)
	775432-776934	BnaA09g01470D	AT4G03280	光合电子转移C Photosynthetic electron transfer C (PETC)
RDW	7333238-7334039	BnaA03g15830D	AT2G34430	捕光叶绿素蛋白复合物II亚基 Light-harvesting chlorophyll-protein complex II subunit B1 (LHB1B1)
	5050885-5053547	BnaA03g11260D	AT5G55700	β-淀粉酶4 Beta-amylase 4 (BAM4)
	5090429-5091422	BnaA03g11320D	AT5G55560	蛋白激酶超家族蛋白 Protein kinase superfamily protein
	6332750-6334339	BnaA03g13820D	AT2G30250	WRKY DNA结合蛋白25 WRKY DNA-binding protein 25 (WRKY25)
	7108373-7109471	BnaA03g15320D	AT2G03610	F-box家族蛋白 F-box family protein

Table 4

Fig. 3

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性状 Trait	亲本 Parents		RIL群体 RIL population					遗传率 Heritability (%)
性状 Trait	ZS11	10D130	均值 Mean	范围 Range	极差 Range value	标准差 SD	变异系数 CV (%)	遗传率 Heritability (%)
RPn	1.048	0.751	0.617^**	0.285-1.589	1.304	0.249	40.357	95.4
RGs	1.040	0.985	0.472^**	0.184-1.521	1.337	0.213	45.127	93.2
RCi	0.957	0.945	0.957^**	0.827-1.106	0.279	0.054	5.643	81.8
RTr	1.860	0.989	0.537^**	0.215-2.324	2.109	0.275	51.210	84.0
RΦPS	0.997	0.920	0.910^**	0.531-1.322	0.791	0.114	12.527	90.7
Rfv	0.857	1.062	0.937^**	0.681-1.114	0.433	0.069	7.364	82.4
Rfv°	1.006	0.971	0.943^**	0.793-1.114	0.321	0.051	5.408	80.0
RqP	0.994	0.952	0.969^**	0.706-1.293	0.587	0.081	8.359	87.0
RETR	0.997	0.920	0.909^**	0.682-1.168	0.486	0.078	8.581	81.8
RNPQ	1.012	0.952	1.733^**	0.952-3.512	2.560	0.498	28.736	89.2
RDW	0.869	1.439	0.551^**	0.828-1.720	1.525	0.270	49.002	97.3

测定指标Trait	RPn	RGs	RCi	RTr	RΦPS	Rfv	Rfv'	RqP	RETR	RNPQ
RGs	0.274^**
RCi	0.036	0.118
RTr	0.272^**	0.584^**	0.272^**
RΦPS	-0.078	0.021	-0.034	0.031
Rfv	-0.047	0.130	0.081	0.204^*	0.188^*
Rfv°	-0.034	-0.061	-0.188^*	0.001	0.427^**	0.060
RqP	-0.032	0.056	0.071	0.003	0.404^**	0.201^*	0.085
RETR	-0.007	0.000	0.001	0.061	0.698^**	0.268^**	0.540^**	0.484^**
RNPQ	-0.003	0.100	0.161	-0.030	-0.304^**	0.012	-0.516^**	-0.095	-0.388^**
RDW	0.197^*	0.038	-0.077	0.087	0.075	-0.030	-0.093	0.125	0.052	0.011

性状 Trait	QTL	标记区间 Marker interval	LOD	表观贡献率 PVE (%)	加性效应 Additive effect	置信区间 Confidence interval (cM)
RPn	qRP_n-A10	AX-177828527-AX182175987	3.18	12.3	-0.0904037	9.474-39.386
RGs	qRg_s-A03-1	AX-95635223-AX-177830413	2.20	8.7	-0.0655227	96.668-100.169
	qRg_s-A03-2	AX-177913067-AX-95507652	2.11	8.3	-0.0643332	106.038-109.129
RCi	qRC_i-C02	AX-86224097-AX-179307085	2.05	8.1	-0.0157819	0.468-3.458
RΦPS	qRΦPS-A06	AX-95503227-AX-177632560	2.84	11.0	0.0472636	145.355-167.417
Rfv	qRf_v-C09	AX-95506407-AX-177910786	2.37	9.3	-0.0296313	38.45-86.514
Rfv°	qRf_v'-A03	AX-95638185-AX-182141998	2.41	9.4	-0.0158728	103.15-118.351
	qRf_v'-A09-1	AX-177835940-AX177912292	2.36	9.2	-0.0166685	87.81-96.271
	qRf_v'-A09-2	AX-95506944-AX-182127697	2.19	8.6	0.0154628	15.138-15.609
RqP	qRqP-A03	AX-95509347-AX-177828121	2.62	10.2	-0.0252692	198.519-228.654
RETR	qRETR-A06	AX-95503227-AX-177632560	2.96	11.4	0.0314658	145.355-167.417
	qRETR-A08	AX-95505568-AX-179307653	2.19	8.6	0.0234311	31.074-45.768
RNPQ	qRNPQ-A09-1	AX-182127697-AX-177632723	2.65	10.3	-0.1639910	15.138-36.592
	qRNPQ-A09-2	AX-177912943-AX-182155511	2.55	9.9	-0.1615910	0-9.664
RDW	qRDW-A03	AX-95502575-AX-182145123	2.37	9.3	-0.0737873	54.689-75.861
	qRDW-A05	AX-95665384-AX-95666447	2.78	10.8	-0.0638520	59.152-61.883

QTL mapping and candidate genes screening of photosynthesis-related traits in Brassica napus L. during seedling stage under aluminum stress

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