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Table of Content

    12 December 2019, Volume 45 Issue 12
    • CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
      Cloning and analysis of BoCDPK14 in self-incompatibility Brasscia olerace
      Xiao-Jing BAI,Xiao-Ping LIAN,Yu-Kui WANG,He-Cui ZHANG,Qian-Ying LIU,Tong- Hong ZUO,Yi-Zhong ZHANG,Qin-Qin XIE,Deng-Ke HU,Xue-Song REN,Jing ZENG,Shao-Lan LUO,Min PU,Li-Quan ZHU
      Acta Agronomica Sinica. 2019, 45(12):  1773-1783.  doi:10.3724/SP.J.1006.2019.94040
      Abstract ( 996 )   HTML ( 95 )   PDF (2173KB) ( 926 )   Save
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      Brasscia oleracea self-incompatibility (SI) is the reaction of rejection or inhibition of stigma to pollens from the same haplotype. Calcium-dependent protein kinase (CDPK) is an important component of plants involved in resistance to stress signals. In this study, we identified an up-regulated gene named BoCDPK14 based on the stigma transcriptome data in 0-60 min self-pollination. The gene was highly homologous to the calcium dependent protein kinase gene involved in stress signaling in Arabidopsis lyrata. BoCDPK14 gene contained an open reading frame (ORF) with the length of 1599 bp, encoding a protein with the length of 533 amino acid residues. It was a hydrophilic protein expressed in cytoplasm, and could be induced to express a 60.4 kD protein in the cytoplasm of E. coli Transetta (DE3). It was suggested that BoCDPK14 is an active cytoplasmic protein. The 2000 bp upstream of BoCDPK14 translation initial codon contained elements for responses to stress, hormone, and metabolic regulation. BoCDPK14 was expressed in stigma, pollen, flower bud, petal, and leaf, with lower expression level in stigma than in pollen. The results of qRT-PCR revealed that BoCDPK14 mRNA expression level after self- and cross-pollinations for 0 min to 60 min was consistent with that of RNA-seq data. The interaction between BoCDPK14 protein kinase domain and glutamate receptor channel protein BoGLR2.8d was identified by yeast two-hybrid, suggesting that BoCDPK14 might be a novel protein involved in SI reaction. These results suggest that BoCDPK14 may act as a Ca 2+ signal element to participate the process of response to pollen stimulation in the stigma of Brasscia oleracea, which provides a new insight into the further research and utilization of self-incompatibility of Brasscia oleracea.

      Genome-wide identification of Laccase gene family in update G. hirsutum L. genome and expression analysis under V. dahliae stress
      Jing ZHAO,Xu-Tong LI,Xue-Zhong LIANG,Zhi-Cheng WANG,Jing CUI,Bin CHEN,Li-Qiang WU,Xing-Fen WANG,Gui-Yin ZHANG,Zhi-Ying MA,Yan ZHANG
      Acta Agronomica Sinica. 2019, 45(12):  1784-1795.  doi:10.3724/SP.J.1006.2019.94053
      Abstract ( 772 )   HTML ( 44 )   PDF (6597KB) ( 609 )   Save
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      Verticillium dahliae stress causes a disease in vascular bundle that decreases cotton yield and fiber quality. During cotton defense against pathogen infection, disease resistance genes play important roles. Laccase is a multifunctional oxidase that plays an important role in lignin synthesis and plant resistance. High-quality cotton reference genome is necessary to improve the accuracy of gene family analysis. In this study, the laccase (GhirLAC) family genes in the update genome of G. hirsutum L. cv. TM-1 were identified by bioinformatics, and its physical and chemical properties, gene structure, chromosome location and expression pattern under V. dahliae stress were analyzed. There were 83 members of GhirLAC family in the genome of G. hirsutum L., which distributed on 24 chromosomes. All GhirLAC proteins predicted were located in extracellular and had the same conserved motif. Phylogenetic analysis showed that the members of the GhirLAC genes family were divided into seven subgroups. According to the analysis results of cotton transcription under V. dahliae stress, it was clear that the expression pattern of GhirLAC genes could be divided into three groups, of which, group 1 and group 2 GhirLAC genes displayed down-regulation and up-regulation expression patterns, respectively, suggesting that these genes should play important roles in cotton Verticillium wilt resistance. Furthermore, we identified three candidate genes expression patterns induced by V. dahliae, including GhirLAC02 (GhLAC4), GhirLAC38 (GhLAC11), and GhirLAC20 (GhLAC12), the qPCR results were consistent with the expression trend based on transcriptome data. This study lays a foundation for further analysis of disease resistance function and molecular mechanism of GhirLAC gene in cotton.

      Growth habit identification and diversity and stability analysis of heading date in Tibetan barley (Hordeum vulgare L.)
      Lhundrupnamgyal,Hui-Hui LI,Gang-Gang GUO, Chemiwangmo,Li-Yun GAO,Ya-Wei TANG, Nyematashi, Dawadondrup, Dolkar
      Acta Agronomica Sinica. 2019, 45(12):  1796-1805.  doi:10.3724/SP.J.1006.2019.91025
      Abstract ( 717 )   HTML ( 28 )   PDF (4299KB) ( 587 )   Save
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      To improve the utilization of Tibetan barley germplasm and select appropriate parents in hybridization breeding, evaluated the growth habit and heading date diversity of 1605 Tibetan barley germplasm under the spring and autumn sowing conditions in Nyingchi and Lhasa, Tibet. The growth habit was determined by the difference in heading date observed in two spring sowing dates in Lhasa, i.e. normal spring sowing I and 10 days later of spring sowing II, showing that 96.2% of the landraces were spring barley distributed in all three ecological regions; 3.8% of the landraces were the winter barley accessions originated mainly from the southeastern ecological region of Tibet. Shannon-Wiener’s analysis showed that the heading date diversity was higher under spring-sown environment than under autumn-sown environment. The heading date diversity was highest in Lhasa region with spring sowing, moderate in Nyingchi spring-sown environment, and lowest in Nyingchi autumn-sown environment. In the same environment, landraces had higher diversity than modern varieties. A total of 368 spring-type landraces and 21 varieties were stable in heading date diversity across environments, and the cultivar Kangqing 3 had the highest stability among all tested materials. This study helps to further understand the growth habits, heading dates diversity and environmental stability of barley resources in Tibet, and provides useful information for the development of broad-adapted barley varieties.

      Genome-wide association analysis and candidate genes prediction of waterlogging-responding traits in Brassica napus L.
      Yang-Yang LI,Rong-Rong JING,Rong-Rong LYU,Peng-Cheng SHI,Xin LI,Qin WANG,Dan WU,Qing-Yuan ZHOU,Jia-Na LI,Zhang-Lin TANG
      Acta Agronomica Sinica. 2019, 45(12):  1806-1821.  doi:10.3724/SP.J.1006.2019.94027
      Abstract ( 907 )   HTML ( 48 )   PDF (9249KB) ( 738 )   Save
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      The yield and quality of rapeseed are significantly affected by waterlogging. Many researches on waterlogging of rapeseed mostly aimed at its mechanism and physiological response, but a few of them identified the genes related to waterlogging. In this study, 248 Brassica napus L. accessions were planted in flowerpots with treatments of waterlogging and well watering for four weeks when the plants had four true leaves in rain-proof installation, and eight waterlogging-responding traits were investigated. The genome-wide association analysis was carried out based on 60K Illumina Infinium SNP genotype data and the candidate genes associated with waterlogging responses were predicted. Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering. The root fresh weight and dry weight were lower in most accessions and higher in some accessions under waterlogging stress. A total of 36 SNPs were identified associated with waterlogging traits and explained the phenotypic variation of 8.28%-12.95%. There were significant (P < 0.05) or extremely significant (P < 0.01) differences in the waterlogging resistance index among genotypes of 17 SNPs. The Blocks containing 17 SNPs covered 71 candidate genes. Among them, 64 candidate genes were homologous to Arabidopsis genes by blast. They encoded transcription factors (e.g. transcription initiation factor IIF, bZIP68, myb-like HTH transcriptional regulator family protein), transport protein (e.g. SUC2), inhibitor of growth (e.g. ING2), protein phosphatase (e.g. protein phosphatase 2C family protein), DNA/RNA binding proteins [e.g. bHLH DNA-binding superfamily protein, RNA-binding (RRM/RBD/RNP motifs) family protein], hormone response proteins (e.g. ARF8, ADC2, ERD6, NF-YC9), oxidative/osmotic/cold stress or water deprivation response proteins (e.g. ERD6, NP1, TIR-NBS-LRR, CRT1b). These results would be benefit for revealing the waterlogging resistance mechanism and cultivating new varieties with waterlogging resistance in Brassica napus L.

      Cloning and analysis of candidate gene resistant to SC3 in soybean
      Wen-Yang XIANG,Yong-Qing YANG,Qiu-Yan REN,Tong-Tong JIN,Li-Qun WANG,Da-Gang WANG,Hai-Jian ZHI
      Acta Agronomica Sinica. 2019, 45(12):  1822-1831.  doi:10.3724/SP.J.1006.2019.94054
      Abstract ( 526 )   HTML ( 15 )   PDF (3930KB) ( 684 )   Save
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      Soybean mosaic virus (SMV) is one of the prevalent pathogens of soybean, causing great reduction of soybean yield worldwide. Soybean disease resistance-breeding is currently the most cost-effective measure to control SMV, and identification of resistance genes is the basis of disease resistance breeding. According to the previous mapping result of resistance gene to SMV strain SC3, two genes (GmR47, GmR51) with TIR-NBS-LRR domain were cloned. Bioinformatics analysis showed that both GmR47 and GmR51 genes have SNP mutations in the susceptible varieties and resistant varieties, and the mutation sites are located in the conserved domain. These two proteins encoded by GmR47 and GmR51 genes are predicted to be Tobacco mosaic virus (TMV) resistant N proteins. The results of homologous alignment between species indicated that GmR47 and GmR51 genes were close to those of wild soybean. The expression of GmR47 and GmR51 was analyzed after inoculation with soybean mosaic virus in soybean, demonstrating that GmR47 and GmR51 could increase the expression level in response to SMV infection, with the higher level in resistant varieties than in susceptible varieties. Analysis of the alternative splicing of GmR47 and GmR51 revealed that the two genes have different splice variants IN1, IN2, and IN3. The response analysis of splices to SMV showed that all splices were able to increase the expression in response to virus induction, with the higher level in resistant varieties than in susceptible varieties. It indicated that these alternative splicing may be involved in the disease resistance process of soybean to SMV. The result of this study lay a foundation for the study of subsequent gene function.

      QTL mapping of adult-plant resistance to stripe rust in wheat variety holdfast
      Fang-Ping YANG,Jin-Dong LIU,Ying GUO,Ao-Lin JIA,Wei-E WEN,Kai-Xiang CHAO,Ling WU,Wei-Yun YUE,Ya-Chao DONG,Xian-Chun XIA
      Acta Agronomica Sinica. 2019, 45(12):  1832-1840.  doi:10.3724/SP.J.1006.2019.91026
      Abstract ( 828 )   HTML ( 12 )   PDF (861KB) ( 444 )   Save
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      Holdfast is an elite wheat cultivar from the United Kingdom with well durable resistance to stripe rust for many years. The aim of the present study was to identify adult-plant resistance (APR) genes to stripe rust in Holdfast and closely linked molecular markers to provide materials and methods for breeding wheat cultivars with durable resistance. A recombinant inbred lines (RIL) population, generated from the cross between Mingxian 169 (highly susceptible) and Holdfast, was planted in Zhongliang and Gangu of Gansu province, and Chengdu of Sichuan province during 2014-2015 and 2015-2016 cropping seasons for evaluating maximum disease severities (MDS) of stripe rust. The chromosomal locations of QTL were firstly determined based on the wheat 660K SNP array and bulked segregant analysis. Then kompetitive allele specific PCR (KASP) markers were developed to genotype the entire RIL population. Finally, the quantitative trait loci (QTLs) mapping of adult-plant resistance to stripe rust in the RIL population were performed, and two QTLs for APR to stripe rust were mapped on chromosomes 5AL and 7AL, respectively. The QTL on chromosome 5AL, designated QYr.gaas-5AL, was identified in all environments, including Zhongliang 2015, Chengdu 2015, Chengdu 2016, Gangu 2015, Gangu 2016, explaining phenotypic variances ranging from 6.5% to 9.3%. QYr.gaas-5AL was flanked by markers Ax-109948955 and Ax-108798241 with genetic distances of 0.5 cM and 1.1 cM, respectively. The 7AL QTL, designated QYr.gaas-7AL, was detected in two environments Gangu 2015 and Gangu 2016, explaining 6.2% and 7.3% of the phenotypic variances, respectively. QYr.gaas-7AL was mapped between markers Ax-110361069 and Ax-108759561 with genetic distances of 0.5 cM and 0.7 cM, respectively. The RILs containing the resistance allele at QYr.gaas-5AL and QYr.gaas-7AL loci were more resistant to stripe rust and had significantly lower MDS than those without the resistance alleles of QYr.gaas-5AL and QYr.gaas-7AL, indicating that QYr.gaas-5AL and QYr.gaas-7Al can effectively reduce stripe rust severities, thus may be used in wheat breeding for improvement of stripe rust resistance.

      Identification and expression analysis of GRF transcription factor family of Chenopodium quinoa
      Pi-Biao SHI,Bing HE,Yue-Yue FEI,Jun WANG,Wei-Yi WANG,Fu-You WEI,Yuan-Da LYU,Min-Feng GU
      Acta Agronomica Sinica. 2019, 45(12):  1841-1850.  doi:10.3724/SP.J.1006.2019.94049
      Abstract ( 824 )   HTML ( 32 )   PDF (3002KB) ( 1507 )   Save
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      Growth-regulating factors (GRFs) are plant-specific proteins which play an important role in regulating plant growth and development. Quinoa is one of the plant sources that can meet human daily nutritional needs and is also considered as one of the most promising crops in the future. However, no systematical study about GRF gene family has been performed in quinoa to date. In this study, the GRFs in the whole genome of quinoa were identified by bioinformatics method, and their physicochemical properties, gene structure, conserved domain, phylogenetic relationship and tissue expression were analyzed. There were 18 GRF transcription factors in quinoa, with the protein length from 77 to 621 aa, the molecular weight from 8.81 to 67.38 kD and the isoelectric point from 5.23 to 9.37. Each member contained 1-4 introns and 2-5 exons. And all 18 GRF proteins possessed highly conserved QLQ domain composed of 31-35 aa or WRC domain composed of 25-43 aa. Phylogenetic analysis showed that the GRF transcription factors were more closely related between quinoa and Arabidopsis than between quinoa and rice. The expression level of GRFs was higher in seed, moderate in inflorescence and root, and relatively lower in other tissues, showing obvious tissue expression specificity.

      TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
      Regulatory effects of coronatine on photosynthetic characteristics and yield of soybean under different irrigation conditions
      Hai-Yue YU,Yan YAN,Yu-Shi ZHANG,Ming-Cai ZHANG,Zhao-Hu LI
      Acta Agronomica Sinica. 2019, 45(12):  1851-1858.  doi:10.3724/SP.J.1006.2019.94035
      Abstract ( 555 )   HTML ( 24 )   PDF (561KB) ( 339 )   Save
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      Drought is one of the important factors limiting the high and stable yield of soybean, and an important problem restricting the sustainable production of soybean in China. Using biological regulators to improve the drought tolerance of soybean in production is a new feasible biological water-saving mode. In this study, the field experiments were conducted to investigate the effects of coronatine (COR) on leaf water potential, photosynthetic characteristics, plant traits, yield and its components of soybean under conventional irrigation and no irrigation conditions from 2015 to 2016. Under conventional irrigation conditions, COR could not change the values of leaf water potential, chlorophyll content, photosynthetic rate, chlorophyll fluorescence parameters, and activities of RuBP carboxylase and SPS in soybean leaves compared to control. And there was no significant difference in yield and biomass between COR treatment and control. However, under no irrigation conditions, COR significantly increased the leaf water potential, chlorophyll content, photosynthetic rate and chlorophyll fluorescence parameters, as well as the activities of RuBP carboxylase and SPS of soybean leaves, thus improved the yield and yield components. These results suggest that COR has a positive regulatory effect on photosynthetic characteristics and yield formation of soybean under no irrigation conditions, and can be used as a new technical means for cultivation preventing drought resistance in soybean production.

      Characteristics and influencing factors of geographical agglomeration of cotton plantation in Xinjiang
      Chun-Yue MA,Sawut Mamat,Ablet Ershat,Jie YAO
      Acta Agronomica Sinica. 2019, 45(12):  1859-1867.  doi:10.3724/SP.J.1006.2019.94041
      Abstract ( 734 )   HTML ( 11 )   PDF (3129KB) ( 585 )   Save
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      Xinjiang is the largest cotton production base in China. The study on the geographical agglomeration characteristics of cotton planting in Xinjiang is of great significance in adjusting and optimizing the agricultural structure distribution of cotton production, increasing farmers’ income, and promoting the sustainable development of cotton. Based on the cotton production data of Xinjiang from 1988 to 2016, we explored the spatial-temporal change characteristics of cotton plantation by Location quotient, Gini coefficient, and spatial autocorrelation analysis, qualitatively and quantitatively analyzed various influence factors on the geographical agglomeration of cotton plantation by spatial panel data model, and revealed the main driving force of cotton planting industry in Xinjiang. The cotton planting area in Xinjiang experienced three stages, including continuous growth (1988-1999), slow decrease (2000-2004) and fluctuating growth (2005-2016). In 2016, the cotton planting area accounted for nearly 3/5 of the total area in China, and its professional agglomeration level was higher than that of other regions in China, with the increasingly strengthened leading position since 1992. The regional characteristics of cotton planting industry in Xinjiang were obvious. The change of cotton area in southern Xinjiang dominated the change of cotton plantation in Xinjiang. The agglomeration level of cotton plantation in Xinjiang showed a trend of slow recovery after a decline in fluctuation since 1988. The high aggregation (H-H) area transferred from Kashgar area to Kuqa and Xinhe in Aksu area, at the same time, the changes of high-low cluster, low-high cluster and low-low cluster were not significant. The main factors promoting the geographical agglomeration of cotton planting in Xinjiang included the proportion of productive land area, cotton comparative income, mechanization level and policy factors.

      Effect of planting density on light interception within canopy and grain yield of different plant types of maize
      Yan-Wen BAI,Yong-Hong YANG,Ya-Li ZHU,Hong-Jie LI,Ji-Quan XUE,Ren-He ZHANG
      Acta Agronomica Sinica. 2019, 45(12):  1868-1879.  doi:10.3724/SP.J.1006.2019.93011
      Abstract ( 1060 )   HTML ( 40 )   PDF (976KB) ( 1406 )   Save
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      The objective of this study was to clarify the relationship between light interception in canopy and dry matter production and grain yield in different plant types of maize. The response of morphological characteristics, canopy light distribution, grain filling parameters and dry matter accumulation were studied using three different maize hybrids Shaandan 609 (SD609, compact), Qinlong 14 (QL14, semi-compact), and Shaandan 8806 (SD8806, flat) with four plant densities (4.5×10 4, 6.0×10 4, 7.5×10 4, and 9.0×10 4plants hm -2) in the field from 2016 to 2017. The average yields of SD609, QL14, and SD8806 were 12,176, 9624, and 8533 kg hm -2, respectively, within two years, reaching high yields under 9.0×10 4, 7.5×10 4, and 6×10 4 plants hm -2, with the yield increase of 26.9%, 20.4%, and 19.7% compared with those under 4.5×10 4 plants hm -2, respectively. With the increase of plant density, leaf area decreased, but LAI and leaf orientation value increased. The middle leaves of SD609 were more upright and larger than those of QL14 under 9×10 4 plants hm -2. With increasing plant density, Dmax (days to the maximum grain-filling rate), Wmax (kernel weight at the maximum grain filling rate), Gmax (maximum grain-filling rate), Gave (average grain-filling rate) and P (active filling period) decreased, the Dmax for SD609 was 1.4 days and 3.0 days earlier than that of QL14 and SD8806, and the Wmax and P were higher than those of SD636 (0.3 g and 3.3 d) and SD8806 (1.1 g and 5.4 d), respectively. The dry matter accumulation after silking and the contribution of dry matter transportation to grain yield increased and then decreased with the increase of plant density, the accumulation, transportation and contribution to grain of dry matter after anthesis were higher in SD609 than QL14 (5.1%, 36.0%, 33.5%) and SD8806 (26.6%, 46.7%, 59.1%). The light interception in the ear canopy was significantly correlated with yield (r = 0.631, P < 0.05), the dry matter accumulation after silking (r = 0.661) and average grain filling rate (r = 0.859) at P < 0.01. Thus, compared with QL14 and SD8806, SD609 could regulate the mid and upper leaves more vertical under close planting, improve the light distribution in the mid and lower canopy, maintain a higher area of green leaves, delay the senescence of canopy leaves, increase dry matter accumulation after anthesis and grain filling rate, so obtain a higher grain yield.

      Nitrogen accumulation, metabolism, and yield of proso millet in proso millet- mung bean intercropping systems
      Ke DANG,Xiang-Wei GONG,Guang-Hua CHEN,Guan ZHAO,Long LIU,Hong-Lu WANG,Pu YANG,Bai-Li FENG
      Acta Agronomica Sinica. 2019, 45(12):  1880-1890.  doi:10.3724/SP.J.1006.2019.94042
      Abstract ( 1222 )   HTML ( 25 )   PDF (505KB) ( 787 )   Save
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      Cereal-legume intercropping is a ecological and efficient planting pattern. To investigate the effects of proso millet- mung bean intercropping on nitrogen accumulation, metabolism and yield of proso millet from the flowering to maturity stage, we designed four patterns of proso millet (P) to mung bean (M) including 2:2, 4:2, 4:4, and 2:4 in 2017 and 2018 in Yulin, Shaanxi province. The intercropping significantly improved the nitrogen contents in root, stem, leaf and sheath of proso millet at the flowering stage. The nitrogen content in panicle at the maturity stage increased by 10.9%-15.9% compared with the sole cropping of proso millet. Intercropping increased the dry matter accumulation by 11.6%-32.1% and the nitrogen accumulation of proso millet by 12.8%-36.9%. The nitrogen transportation from leaves and stems to panicles increased by 51.7%-78.9% and 24.1%-55.6%, respectively. The proso millet-mung bean intercropping significantly promoted the nitrogen metabolism of proso millet flag leaves and roots. The nitrate reductase activity, glutamine synthase activity, soluble protein content and free amino acid content increased to a different degree, with the maximum in 2P4M treatment. The productivity and yield were enhanced in intercropping by boosting physiological metabolism and nitrogen assimilation. Overall, these results suggest that proso millet-mung bean intercropping pattern enhances the nitrogen accumulation, metabolism, and yield of proso millet, and 2P4M treatment is optimal to improve nitrogen uptake and yield under proso millet/mung bean intercropping systems in Yulin, Shaanxi.

      Establishment and applicant of near-infrared reflectance spectroscopy models for predicting main fatty acid contents of single seed in peanut
      Jian-Guo LI,Xiao-Meng XUE,Zhao-Hua ZHANG,Zhi-Hui WANG,Li-Ying YAN,Yu-Ning CHEN,Li-Yun WAN,Yan-Ping KANG,Dong-Xin HUAI,Hui-Fang JIANG,Yong LEI,Bo-Shou LIAO
      Acta Agronomica Sinica. 2019, 45(12):  1891-1898.  doi:10.3724/SP.J.1006.2019.94016
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      Profile of fatty acid is the main factor determining the nutritional value and shelf life of peanut. High oleate peanut has been increasingly favored by customers and peanut processing enterprises, as it provides a prolonged shelf-life and a beneficial effect on human health. Therefore, creating high oleate peanut cultivar is an important objective for peanut breeding. Developing a nondestructive method which could detect the fatty acid composition in peanut rapidly, efficiently and accurately is an important technical support for accelerating the processing. In this study, models for predicting the contents of main fatty acids (oleic acid, linoleic acid and palmitic acid) in a single peanut seed were built up using near-infrared reflectance spectroscopy (NIRS). The coefficient of multiple determination (R 2) and the root mean squared error of external calibration (RMSECV) of prediction models were 0.907 and 3.463 for oleic acid, 0.918 and 2.824 for linoleic acid, 0.824 and 0.782 for palmitic acid, respectively. One hundred peanut seeds were analyzed by both NIR and gas chromatography (GC) to validate the accuracy of the prediction models. The correlation coefficients of oleic acid, linoleic acid and palmitic acid between NIR values and GC values were 0.88, 0.90, and 0.71, respectively, suggesting that these models could accurately predict the contents of the three main fatty acids in a single peanut seed. Furthermore, based on these prediction models, a breeding method of high oleate peanut without assistance of molecular marker was developed, and a high oleate peanut cultivar Zhonghua 215 was successfully bred.

      RESEARCH NOTES
      Analysis method of wheat germination metabolomics based on UPLC-QTOF/ MS
      Li-Na WANG,Bu-Jun WANG
      Acta Agronomica Sinica. 2019, 45(12):  1899-1904.  doi:10.3724/SP.J.1006.2019.91017
      Abstract ( 857 )   HTML ( 12 )   PDF (379KB) ( 612 )   Save
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      To investigate the effects of different extraction methods on the extraction of wheat metabolites in germination, we established a pre-treatment and analysis method for the metabolomics samples based on UPLC-QTOF/MS. In this study, we used Zhoumai 26 grains germinated for 2 days, and designed L9(3 4) orthogonal experiments with extraction solvents, extraction methods and extraction time three levels based on UPLC-QTOF/MS. Besides, the best combination of extraction was obtained by principal component and cluster analysis: 1609 metabolic peaks were detected by 80% acetonitrile (0.1% formic acid) with shocking for 30 min, indicating that the extraction solvent plays a major role in the extraction of metabolites. A total of 92 wheat metabolites were identified in this study.

      Development and validation of the functional marker of grain weight-related gene TaCYP78A5 in wheat (Triticum aestivum L.)
      Wen-Jie SI,Lin-Nan WU,Li-Jian GUO,Meng-Die ZHOU,Xiang-Li LIU,Meng MA,Hui-Xian ZHAO
      Acta Agronomica Sinica. 2019, 45(12):  1905-1911.  doi:10.3724/SP.J.1006.2019.91016
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      In order to develop the functional marker of wheat grain weight related gene TaCYP78A5 (Triticum aestivum Cytochrome P450 78A5) and excavate superior alleles for 1000-grain weight, functional marker CAPS-5Ap was developed according to the different SNP loci of TaCYP78A5-2A by analyzing the TaCYP78A5 promoter sequence of 30 wheat varieties and alignment evaluation. There were five SNP loci in TaCYP78A5-2A promoter regions of 30 wheat varieties, which could be divided into two kinds of genotype, TaCYP78A5-2Ap-HapIand TaCYP78A5-2Ap-HapII. The frequency of TaCYP78A5-2Ap-HapI and TaCYP78A5-2Ap-HapII in 323 modern varieties of wheat was 17.96% and 82.04% respectively, which indicating that the CAPS-5Ap can be used to identify two genotypes of TaCYP78A5-2A promoter sequence in wheat. What's more, the correlation between the genotype data and 1000-grain weight phenotype data of 323 modern varieties was analyzed, showing that CAPS-5Ap marker associated with grain weight, and TaCYP78A5-2Ap-HapII was the superior genotype for 1000-grain weight. These results may provide useful information for molecular marker assisted selection and character improvement in breeding for high yield wheat.

Co-sponsored:
the Crop Science Society of China
the Institute of Crop Science, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Chen Xiao-ya Yang Jian-chang Zhang Xian-long Wang Jian-kang Xu Ming-liang Liu Chun-ming Wang Dao-wen Sun Chuan-qing Ding Yan-feng Jin Wei-wei Chu Cheng-cai Cheng Wei-hong
Director of the editorial department:
Yan Chun-ling
CN 11-1809/S
ISSN 0496-3490
Post subscription code: 82-336

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  • Started in 2013
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Editor in chief: Wan Jian-min
CN 10-1112/S
ISSN 2095-5421, 2214-5141(online)
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