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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1030-1037.doi: 10.3724/SP.J.1006.2009.01030


Global Genome Expression Analysis of Transcription Factors under PEG Osmotic Stress in Rice Root System

MA Ting-Chen12,CHEN Rong-Jun2,YU Rong-Rong2,ZENG Han-Lai1,ZHANG Duan-Pin1*   

  1. 1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University,Wuhan 430070,China;2Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China
  • Received:2008-12-10 Revised:2009-02-17 Online:2009-06-12 Published:2009-04-16
  • Contact: ZHANG Duan-Ping,E-mail:tingchenm@163.com


Transcription factors play an important role in plant stress tolerance. The Affymetrix rice genome arrays were used to research the expression change of transcription factors and their families in two rice cultivars with different levels of drought tolerance under PEG osmotic stress. Results showed that a total of 95 transcripts encoding transcription factors (24 transcripts were down-regulated and 71 transcripts were up-regulated at the transcription level) in Xingfengzao 119 and 129 transcripts (69 transcripts were down-regulated and 60 transcripts were up-regulated at the transcription level) in Aihua 5 were affected by PEG osmotic stress. Transcription factors transcripts in response to PEG osmotic stresses were belonged to 30 transcription factors families for each of two cultivars, but there was a difference between the two 30 families. The transcription factors transcripts for expressed cultivar specific-response to PEG osmotic stress, with 72 transcripts for Xingfengzao 119 and 106 for Aihua 5. There was an overlap of transcripts in response to PEG osmotic stress between Xingfengzao 119 and Aihua 5, with the 16 and 7 up-regulated and the down-regulated at the transcription level, respectively. Distribution of PEG osmotic stress regulating genes of two cultivars on rice chromosome was different and their transcripts overlapped were located in 0.432 to 26.139 Mb of chromosome 2 and 0.076 to 20.597 Mb of chromosome 5.

Key words: Rice, Root system, Microarray, Transcription factors, Different drought tolerance

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