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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 233-241.doi: 10.3724/SP.J.1006.2010.00233

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Introgressed Segments in Near-Isogenic Lines Carrying Soybean Maturity Genes

WEI Jin-Peng1,**,E Wen-Di2,**,LIU Zhang-Xiong1,GUAN Rong-Xia1,CHANG Ru-Zhen1,QIU Li-Juan1*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 China Agricultural Technology Extension Service Center, Beijing 100026, China
  • Received:2009-05-26 Revised:2009-10-02 Online:2010-02-10 Published:2009-12-21
  • Contact: QIU Li-Juan,E-mail: qiu_lijuan@263.net;Tel:010-82105843

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Abstract:

It is very important to exploit the soybean maturity genes for developing varieties with high-yield and wide adaptability in both theory and practice. The objective of the study was to identify the size and position of introgressed segments associating with maturity genes, which provides information of fine mapping and cloning maturity genes. Twenty-three introgression lines (near isogenic lines, NILs) carrying four soybean maturity genes and their recurrent parent, were analyzed with 243 SSR markers, and 266 introgression segments were found, so each NIL containing 11.6 introgressed segments on average. The majority of 150 introgressed segments with E1, were located on chromosome 6, while 55, 49; 73 introgressed segments with E2, e3, E5on chromosomes 20, 12 and 20 respectively. The NILs with the same maturity gene trended to cluster together. The results showed that E1 was related to the interval of satt643–sat_312 on chromosome 6 and sat_095 on chromosome 11; both E2 and E5 loci associated with the interval of satt587–satt496 on chromosome 20; e3 was related to the interval of satt317–satt181 on chromosome 12. Therefore, E1 locus was validated with NILs, anda new markerrelated to E1 was detected, and E2, e3, E5 gene-related markers were identified.

Key words: Soybenan, Near-isogenic lines, Introgressed segments, Maturity


[1] McBlain B, Bernard R L. A new gene affecting the time of flowering and maturity in soybean. J Hered, 1987, 78: 160-162

[2] Bernard R L.Two genes for time of flowering and maturity in soybeans. Crop Sci, 1971, 11: 242-244

[3] Buzzell R I. Inheritance of a soybean flowering response to fluorescent-daylength conditions. Can J Genet Cytol, 1971, 13: 703-707

[4] Buzzell R I, Voldeng H D. Inheritance of insensitivity to long daylength. Soybean Genet News, 1980, 7: 26-29

[5] Bonato E R, Vello N A. E6, a dominant gene conditioning early flowering and maturity in soybeans. Genet Mol Biol, 1999, 22: 229-232

[6] Cober E R, Voldeng H D. A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T. Crop Sci, 2001, 41: 698-701

[7] Ray J D, Hinson K, Mankono J E B, Malo M F. Genetic control of a long-juvenile trait in soybean. Crop Sci, 1995, 35, 1001-1006

[8] Chang R-Z(常汝镇). A genetic analysis on effect of maturity genes in soybeans. Soybean Sci (大豆科学), 1992, 11(2): 127-133 (in Chinese with English abstract)

[9] Chang R-Z(常汝镇), Li X-H(李星华). Study on effect of maturity genes in soybeans under summer sowing condition. Chin J Oil Crop Sci (中国油料), 1993, 15(3): 15-17 (in Chinese with English abstract)

[10] Liu B H, Kanazawa A, Matsumura H, Takahashi R, Harada K, Abe J. Genetic redundancy in soybean photoresponses associated with duplication of the phytochrome a gene. Genetics, 2008, 180: 995-1007

[11] Wang Y(王英), Han T-F(韩天富). Study on the heredity and QTL mapping of growth period traits in soybean. J Anhui Agric Sci (安徽农业科学), 2008, 36(4): 1391-1393 (in Chinese with English abstract)

[12] Zhao Y-F(赵永锋), Chen J-T(陈景堂), Zhu L-Y(祝丽英), Jia X-Y(贾晓艳), Huang Y-Q(黄亚群), Liu Z-Z(刘志增). Status of research on application and establishment of SSILs in maize. J Maize Sci (玉米科学), 2006, 14(3): 17-19 (in Chinese with English abstract)

[13] Paterson A H, Deverna J W, Lanini B, Tanksley S D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes , in an interspecies cross of tomato. Genetics, 1990, 124: 735-742

[14] Yamamoto T, Kuboki Y, Lin S Y, Sasaki T, Yano M. Fine mapping of quantitative trait loci Hd1, Hd2, and Hd3, controlling heading date of rice, as single mendelian factors. Theor Appl Genet, 1998, 97: 37-44

[15] Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene, CONSTANS. Plant Cell, 2000, 12: 2473-2483

[16] Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield- associated QTL. Genetics, 1995, 141: 1147-1162

[17] Xu H-S(徐华山), Sun Y-J(孙永建), Zhou H-J(周红菊), Yu S-B(余四斌). Development and characterization of contiguous segment substitution lines with background of an elite restorer Line. Acta Agron Sin (作物学报), 2007, 33(6): 979-986 (in Chinese with English abstract)

[18] Meksem K, Pantazopoulos P, Njiti V N, Hyten L D, Arelli P R, Lightfoot D A. ‘Forrest’ resistance to the soybean cyst nematode is bigenic: saturation mapping of the Rhg1 and Rhg4 loci. Theor Appl Genet, 2001, 103:710-717

[19] Maughan P J, Sagllai-Maroof M A, Buss G R, Huestis G M. Amplified fragment length polymorphism (AFLP) in soybean: Species diversity, inheritance, and near-isogenic line analysis. Theor Appl Genet, 1996, 93: 392-401

[20] Meksem K, Doubler T W, Chancharoenchai K, Nijti N, Chang S J, Rao Arelli A P, Cregan P E, Gray L E, Gibson P T, Lightfoot D A. Clustering among loci underlying soybean resistance to Fusarium solani, SDS and SCN in near-isogenic lines. Theor Appl Genet, 1999, 99: 1131-1142

[21] Chowdhury A K, Srinives P, Saksoong P, Tongpamnak P. RAPD markers linked to resistance to downy mildew disease in soybean. Euphytica, 128: 55-60, 2002

[22] Cairo C A, Stein J, Delgado L, Bortolotti S, Guelman S A, Ortiz J P A, Morandi E N. Tagging the juvenile locus in soybean (Glycine max L. Merr.) with molecular markers. Euphytica, 2002, 124: 387-395

[23] Li Y-X(李永祥), Liu C(刘成), Shi Y-S(石云素), Shen H-B(申海兵), Song Y-C(宋燕春), Wang T-Y(王天宇), Li Y(黎裕). Development and evaluation of maize introgression lines with drought tolerance in flowering-time. J Plant Genet Resour (植物遗传资源学报), 2008, 9(3): 293-296 (in Chinese with English abstract)

[24] Cregan P B, Jarvik T, Bush A L, Shoemaker R C, Lark K G, Kahler A L, Kaya N, VanToai T T, Lohnes D G, Chung J, Specht J E. An integrated genetic linkage map of the soybean genome. Crop Sci, 1999, 39: 1464-1490

[25] Liu G-M(刘冠明), Li W-T(李文涛), Zeng R-Z(曾瑞珍), Zhang G-Q(张桂权). Development of single segment substitution lines (SSSLs) of subspecies in rice. Chin J Rice Sci (中国水稻科学), 2003, 17(3): 201-204 (in Chinese with English abstract)

[26] Keim P, Diemb W, Olson T C, Shoemaker R C. RFLP mapping in soybean association between marker loci and variation in quantitative traits. Genetics, 1990, 126: 735-742

[27] Yamanaka N, Ninomiya S, Hoshi M, Tsubokura Y, Yano M, Nagamura Y, Sasaki T, Harada K. An informative linkage map of soybean reveals QTLs for flowering time, leaflet morphology and regions of segregation distortion. DNA Res, 2001, 8: 61-72

[28] Yamanaka N, Watanabe S, Toda K, Hayashi M, Fuchigami H, Takahashi R, Harada K. Fine mapping of the FT1 locus for soybean flowering time using a residual heterozygous line derived froma recombinant inbred line. Theor Appl Genet, 2005, 110: 634-639

[29] Molnar S J, Rai S, Charette M, Cober E. Simple sequence repeat (SSR) markers linked to E1, E3, E4, and E7 maturity genes in soybean. Genome, 2003, 46: 1024-1036

[30] Mamsur L M, Orf J H, Chase K, Jarvik T, Cregan P B, Lark K G. Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci, 1996, 36:1327-1336

[31] Mansur L M, Lark K G, Kross H, Oliveira A. Interval mapping of quantitative trait loci for reproductive morphological and seed traits of soybean ( Glycine max L. Merr.). Theor Appl Genet, 1993, 86: 907-913

[32] Orf J H, Chase K, Jarvik T, Cregan P B, Adler F R. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642-1651

[33] Zhang W K, Wang Y J, Luo G Z, Zhang J S, He C Y, Wu X L, Gai J Y, Chen S Y. QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) map and their association with EST markers. Theor Appl Genet, 2004, 108: 1131-1139

[34] Tasma I M, Lorenzen L L, Green D E, Shoemaker R C. Mapping genetic loci for flowering time, maturity, and photoperiod insensitivity in soybean. Mol Breed, 2001, 8: 25-35

[35] Ning H-X(宁慧霞), Li Y-H(李英慧), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Guan R-X(关荣霞), Luo Y-P(罗淑萍), Qiu L-J(邱丽娟). Deducing maturity genotype of the Chinese soybean varieties. Acta Agron Sin 作物学报), 2008, 34(3): 382-388 (in Chinese with English abstract)(

[36] Li W-T(李文涛), Zeng R-Z(曾瑞珍), Zhang Z-M(张泽民), Zhang G-Q(张桂权). Analysis of introgressed segments in near-isogenic lines for F1 pollen sterility in rice. Chin J Rice Sci (中国水稻科学), 2003, 17(2): 95-99 (in Chinese with English abstract)

[37] Wang D, Graef G L, Procopiuk A M, Diers B W. Identification of putative QTL that underlie yield in interspecific soybean backcross populations. Theor Appl Genet. 2003, 108: 458-467

[38] Cober E R. Voldeng H D. A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T. Crop Sci, 2001, 41: 698-701

[39] Cober E R, Voldeng H D. Low R: FR light quality delays flowering of E7E7 soybean lines. Crop Sci, 2001, 41: 1823-1826

[40] Hisakazu M, Liu B H, Jun A, Takahashi R. AFLP mapping of soybean maturity gene E4. J Hered, 2008: 1-5

[41] Abe J, Xu D H, Miyano A, Komatsu K, Kanazawa A, Shimamoto Y. Photoperiod-insensitive Japanese soybean landraces differ at two maturity loci. Crop Sci, 2003, 43: 1300-1304
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