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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 1927-1934.doi: 10.3724/SP.J.1006.2013.01927

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Resistance Spectrum Difference between Two Broad-spectrum Blast Resistance Genes, Pigm and Pi2, and Their Interaction Effect on Pi1

YU Miao-Miao1,2,3,DAI Zheng-Yuan1,PAN Cun-Hong1,CHEN Xi-Jun2,YU Ling1,ZHANG Xiao-Xiang1,LI Yu-Hong1,XIAO Ning1,GONG Hong-Bing3,SHENG Sheng-Lan3,PAN Xue-Biao2,ZHANG Hong-Xi1,LI Ai-Hong1,*   

  1. 1 Lixiahe Agricultural Research Institute of Jiangsu Province / Yangzhou Station of Nanjing Sub-center of National Rice Improvement Center, Yangzhou 225007, China; 2 Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 3 Zhenjiang Institute of Agricultural Science in Jiangsu Hilly Region, Jurong 212400, China
  • Received:2013-03-21 Revised:2013-06-24 Online:2013-11-12 Published:2013-08-12
  • Contact: 李爱宏, E-mail: yzlah@126.com; Tel: 0514-87302340

Abstract:

Pigm, an R gene to rice blast disease, is either allelic or tightly linked to Pi2. In this study, a series of near-isogenic lines (NILs) containing Pigm and Pi2 under four genetic backgrounds of rice cultivars were established, respectively. The results from inoculating with 204 representative races at seedling stage showed that the resistance frequency of NILs carryingPigm and Pi2 all reachedmore than70%, but the overlapping degree of resistance spectrum between them was only 54.4% to 65.7%, and the resistance frequencyof hybrids pyramiding Pi1/Pigm and Pi1/Pi2 was more than 90%. Disease development from artificial inoculation at heading stage and natural induction in disease nursery showed consistent trend with that from inoculation at seedling stage. Agronomic traits of most NILs were similar to those of recurrent parent, indicating that the target region contains few linkage drags. In conclusion, Pigm is a broad-spectrum blast resistance gene possessing significant resistance spectrum difference from Pi2, and holds important value for rice blast breeding.

Key words: Blast resistance gene, Marker-assisted selection, Near-isogenic lines, Resistance spectrum, Interaction effect

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