关键词: 水稻, 白化转绿基因, 近等基因系, 农艺性状, 外观米质, 多态性分析

Abstract:

Chlorophyll content mutants are important genetic material for photosynthesis and cytoplasmic-nuclear interaction studies, and useful breeding materials for identifying and improving the purity of hybrid seeds. A variant of green-revertible albino (gra) was discovered among the progenies from a crossing of G46B (indica)×Sirio (japonica). A pair of near-isogenic lines (NILs) of Gra and gra were bred by continuously heterozygote self-crossing. The near-isogenic level was evaluated through analysis of polymorphism detected by 120 InDels markers and the results indicated that Gra and gra is a good pair of NILs. Comparative analysis on agronomic and appearance quality traits of NILs indicated that the gra(t)-gene reduced half of the content of chlorophyll at 4–5th leaf stage, and significantly decreased the white root number at transplanting stage, leaf producing speed, tillering ability as well as available spike number and yield per plant. The gra(t)-gene also significantly influenced the length of 2, 3–5, 7, and 9–11th leaves and the width of 3–7 and 9th leaves. However, the gra(t) gene didn’t affect the other agronomic traits and the appearance quality of the milled rice investigated in this study.

Key words:

Rice, gra(t)-gene, NILs, Agronomic traits, Appearance quality, Polymorphism analysis