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作物学报 ›› 2009, Vol. 35 ›› Issue (9): 1662-1671.doi: 10.3724/SP.J.1006.2009.01662

• 耕作栽培·生理生化 • 上一篇    下一篇

我国玉米自交系叶片保绿性及其与产量的关系

刘开昌1,董树亭2,赵海军1,王庆成1,*,李宗新1,刘霞1,张慧1   

  1. 1山东省农业科学院玉米研究所，山东济南250100；2山东农业大学作物学国家重点实验室，山东泰安271018
  • 收稿日期:2008-12-31 修回日期:2009-04-23 出版日期:2009-09-12 网络出版日期:2009-07-04
  • 通讯作者: 王庆成, E-mail: qcwang@saas.ac.cn; Tel: 0531-83179402
  • 基金资助:

    本研究由国家“十一五”科技支撑计划项目（2006BAD02A09，2007BAD89B09，2007BAD31B03），山东省农业科学院博士基金项目（2007YBS002），山东省农业科学院高技术自主创新基金（2007YCX024）资助。

Leaf Stay-Green Traits in Chinese Maize Inbred Lines and Their Relationship with Grain Yield

LIU Kai-Chang1,DONG Shu-Ting2,ZHAO Hai-Jun1,WANG Qing-Cheng1,*,LI Zong-Xin1,LIU Xia1,ZHANG Hui1   

  1. 1 Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2008-12-31 Revised:2009-04-23 Published:2009-09-12 Published online:2009-07-04
  • Contact: WANG Qing-Cheng, E-mail: qcwang@saas.ac.cn; Tel: 0531-83179402

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3705

被引次数

51

摘要：

选用我国75个常用不同基因型玉米自交系，对其叶片保绿性参数进行了定点动态测定。结果表明，不同自交系抽丝后叶片保绿度的变化均符合方程y = aeb-cx/ (1+eb-cx)，成熟期的绿叶数、成熟期叶绿素含量和相对绿叶面积平均衰减速率(Vm)可作为区分玉米保绿型与非保绿型的关键指标。按照Hiechical聚类分析方法，筛选出12个保绿型自交系，其成熟期相对绿叶面积在60%以上，其Vm平均值为0.687% d-1，在生长季内相对绿叶面积无大幅度衰减，成熟期绿叶数多，叶绿素含量较高；其余63个为非保绿型自交系，还可分为植株叶片衰老较快型与植株叶片衰老较慢型两个亚类。不同自交系抽丝后叶片保绿性与叶面积持续期、单株产量均呈正相关。保绿型的叶面积持续期和单株产量比非保绿型分别高20.02%~23.87%和50.44%~59.38%；与非保绿型自交系相比，保绿型在籽粒灌浆期绿叶面积大，叶绿素含量高，群体光合速率高，光合作用时间长，因而生物产量较高。

关键词: 玉米, 自交系, 保绿性, 产量

Abstract:

Leaf stay-green trait is one of the major targets in maize (Zea mays L.) breeding. Although sugar concentration in culm, green leaf number, leaf area, and chlorophyll content at maturity have been used to evaluate the stay-green trait of maize in earlier studies, there is no a common criterion. To further study the evaluation system for stay-green in maize and disclose the relationship of stay-green with grain yield, we sampled 75 inbred lines from common parents of Chinese maize cultivars. At silking stage and 10, 20, 30, 40, 50, and 60 d after silking, the leaf area (LA), leaf area duration (LAD), relative leaf area, and relative green-leaf area (RGLA) were measured. The results showed that the changes of RGLA after silking accorded with the equation of y = aeb-cx/ (1+eb-cx). On the basis of correlation analysis, the green leaf number, chlorophyll content at physiological maturity, and mean decreasing rate of RGLA (Vm) were selected as the key indices to discriminate the stay-green and non-stay-green maize genotypes. According to relative green leaf area at physiological maturity (MRGLA), the maximum decrease rate of RGLA (Vmax), and the mean decreasing rate of RGLA (Vm), the 75 inbred lines were classified into stay-green and non-stay-green two types with Hiechical clustering analysis. The stay-green type was composed of 12 inbred lines with the following common characteristics: MRGLA of more than 60%, Vm of 0.687% d-1, no significant decrease of RGLA during the whole growing period (67.07% at maturity), and high green leaf number (8.8 leaves) and chlorophyll content (4.43 mg dm-2) at physiological maturity. The non-stay-green type, consisting of 63 inbred lines, was further categorized with quick-leaf-senescence (50 inbred lines) and slow-leaf-senescence (13 inbred lines) subgroups. In the quick- and slow-leaf senescence subgroups, the RGLA at maturity, Vm, green leaf number at maturity, and chlorophyll content at maturity were 17.75% and 33.55%, 1.89% d-1 and 1.44% d-1, 3.2 and 6.2, and 2.06 mg dm-2 and 3.17 mg dm-2, respectively. At physiological maturity, the RGLA was positively correlated with LAD (r= 0.8861, P < 0.01) and yield per plant (r = 0.8221, P < 0.01). The LAD and yield per plant were 20.02–23.87% and 50.44–59.38% higher in the stay-green type than in the non-stay-green type, respectively. Thus, the stay-green type had higher yield potential due to larger green leaf area, higher chlorophyll content and photosynthesis efficiency as well as longer photosynthesis duration.

Key words: Maize, Inbred line, Stay-green, Yield

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