欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1851-1857.doi: 10.3724/SP.J.1006.2009.01851

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

以分子标记辅助选择育成抗条纹叶枯病水稻新品种“武陵粳1号”

潘学彪1*，陈宗祥1，左示敏1，张亚芳1，吴旭江1，马宁1，江祺祥2，阙金华3，周春和3   

  1. 1扬州大学作物遗传生理江苏省重点实验室/植物功能基因组学教育部重点实验室，江苏扬州225009；2江苏省常州市武进区稻麦育种场，江苏武进213149；3江苏省种子管理站，江苏南京210036
  • 收稿日期:2009-02-02 修回日期:2009-04-27 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 潘学彪, E-mail: shuidao@yzu.edu.cn
  • 基金资助:

    本研究由国际科技支撑计划项目（2006BAD01A01-5）和引进国际先进农业科学技术计划（948计划）重大项目（2006-G51）资助。

A New Rice Cultivar“Wulingjing1” with Resistance to Rice Stripe Virus Bred by Marker Assisted Selection

PAN Xue-Biao1,*,CHEN Zong-Xiang1,ZUO Shi-Min1,ZHANG Ya-Fang1,WU Xu-Jiang1,MA Ning1,JIANG  Qi-Xiang2,QUE Jin-Hua3,ZHOU  Chun-He3   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Education Ministry, Yangzhou University, Yangzhou 225009, China; 2 Wujin Rice and Wheat Breeding Station, Changzhou 213149, China; 3 Seeds Management Station of Jiangsu Province, Nanjing 210036, China
  • Received:2009-02-02 Revised:2009-04-27 Published:2009-10-12 Published online:2009-08-07
  • Contact: PAN Xue-Biao, E-mail: shuidao@yzu.edu.cn

PDF

1761

被引次数

31

摘要：

条纹叶枯病是21世纪以来江苏省最重要的水稻病害之一。食味品质较优的主栽品种武育粳3号因高度感染该病而种植面积锐减。本研究以镇稻88为抗条纹叶枯病毒病基因Stvb-i的供体亲本，采用回交育种策略，改良武育粳3号的抗条纹叶枯病性能。在连续回交和自交过程中，以紧密连锁的双侧分子标记对Stvb-i进行“前景”选择，同时对后代与轮回亲本遗传背景的相似程度进行分子标记辅助“背景”选择，在短期内育成抗条纹叶枯病的“武育粳3号”，命名为“武陵粳1号”。新品种保持了原品种的基本农艺性状、丰产性、稳产性和优异的食味品质，并大幅度提高了其条纹叶枯病抗性水平，在江苏省多点抗性鉴定试验中的平均病株率仅为4.4%，极显著低于原品种(53.2%)。

关键词: 水稻条纹叶枯病, 抗性基因供体, 回交育种, 轮回亲本, 标记辅助选择

Abstract:

Rice stripe virus disease is one of the most serious diseases in rice production area of Jiangsu province since 2002. A commercial rice cultivar with excellent food taste-quality, Wuyujing 3, was highly susceptible to the disease and was improved through the MAS strategy in a backcross project with “Zhendao 88” as donor parent of resistance gene Stvb-i. In each backcross generation, we selected the progenies with Stvb-i based on the closely flanked markers. In advanced backcross generations, the marker’s detection for genetic background of selected plants was performed, along with the estimate by the eye for the similarity of selected plants to recurrent parent in synthetic agronomic traits. As a result, a new cultivar, Wulingjing 1, was fleetingly bred in BC3F4 generation, whose resistance to the disease was significantly increased and main characters especially on excellent taste-quality were consistent with those of the recurrent parent Wuyujing 3. In resistance identification tests with multiple locations organized by Seed Management Station of Jiangsu Province, the average percent of affected plants of the new cultivar was only 4.4% and significantly lower than that of recurrent parent (53.2%).

Key words: Rice stripe disease, Resistance gene donor, Backcross breeding, Current parent, MAS

[1] Chen Z-D(陈志德), Zhong W-G(仲维功), Yang J(杨杰), Wang J(王军). Study on the yielding traits and quality characters of Wu yujing 3 and the suggestion for rice breeding. J金陵科技学院学报), 2007, 23(4): 50-53 (in Chinese with English abstract)(

[2] Jiang Q-X(江祺祥), Yang M-F(杨明方), Wang Z-M(王子明), Qian Y-X(钱永祥). A new japonica rice Wuyujing 3 with middle length of the whole growth stage, its breeding and commercial spread. J Jiangsu Agric Sci (江苏农业科学), 1993, (3): 8-10 (in Chinese)

[3] Lu B-G(卢白关), Xu D-Y(徐大勇), Fang Z-W(方兆伟), Qin D-R(秦德荣), Liu H-Q(刘汉青), Pan J-W(攀技伟). Investigation of rice stripe disease developed on some japonica rice cultivars. J Jiangsu Agric Sci (江苏农业科学), 2006, (4): 43-45 (in Chinese)

[4] Chen T(陈涛), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Lin J(林静), Zhang S-B(张所兵), Wang C-L(王才林). Research progress of resistant genetics and breeding against rice stripe disease. J Jiangsu Agric Sci (江苏农业科学), 2006, (2): 1-4 (in Chinese)

[5] Hayano Y, Saito K, Nakamura S, Kawasaki S, Iwasaki M. Fine physical mapping of the rice stripe resistance gene locus, Stvb-i. Theor Appl Genet, 2000, 101: 59-63

[6] Hayano Y, Tsuji T, Fujii K, Saito K, Iwasaki M, Saito A. Loca-lization of the rice stripe disease resistance gene Stvb-i by graphical genotyping and linkage analyses with molecular markers. Theor Appl Genet, 1998, 96: 1044-1049

[7] Sun D-Z(孙黛珍), Jiang L(江玲), Zhang Y-X(张迎信), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Wan J-M(万建民). Detection of QTLassociated with rice stripe resistance in cultivar IR24. Acta Agron Sin (作物学报), 2007, 33(1): 25-30 (in Chinese with English abstract)

[8] Ding X-L(丁秀兰), Jiang L(江玲), Liu S-J(刘世家), Wang C-M(王春明), Chen L-M(陈亮明), Cheng Z-B(程兆榜), Fan Y-J(范永坚), Zhou Y-J(周益军), Wan J-M(万建民). QTL analysis for rice stripe disease resistance gene using recombinant inbred lines (RILs) derived from crossing of Kinmaze and DV85. Acta Genet Sin (遗传学报), 2004, 31(3): 287-292 (in Chinese with English abstract)

[9] Wu S J, Zhong H, Zhou Y, Zuo H, Zhou L H, Zhu J Y, Ji C Q, Gu S L, Gu M H, Liang G H. Identification of QTLs for the resistance to rice stripe virus in the indica rice variety Dular. Euphytica, 2009, 165: 557-567

[10] Pan X-B(潘学彪), Liang G-H(梁国华), Chen Z-X(陈宗祥), Zhang Y-F(张亚芳). Breeding strategy on resistance to rice stripe in Jiangsu. J Jiangsu Agric Sci (江苏农业科学), 2005, (5): 22-23 (in Chinese) Pan C-H(潘存红), Wang Z-B(王子斌), Ma Y-Y(马玉银), Yin Y-J(殷跃军), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Chen Z-X(陈忠祥), Pan X-B(潘学彪). InDel and SNP markers and their application in map-based cloning of rice genes. Chin J Rice Sci (中国水稻科学), 2007, 21(5): 447-453 (in Chinese with English abstract)
[1] 马红勃, 刘东涛, 冯国华, 王静, 朱雪成, 张会云, 刘静, 刘立伟, 易媛. 黄淮麦区Fhb1基因的育种应用[J]. 作物学报, 2022, 48(3): 747-758.
[2] 韩玉洲, 张勇, 杨阳, 顾正中, 吴科, 谢全, 孔忠新, 贾海燕, 马正强. 小麦株高QTL Qph.nau-5B的效应评价[J]. 作物学报, 2021, 47(6): 1188-1196.
[3] 黄义文, 代旭冉, 刘宏伟, 杨丽, 买春艳, 于立强, 于广军, 张宏军, 李洪杰, 周阳. 小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系[J]. 作物学报, 2021, 47(11): 2080-2090.
[4] 胡茂龙, 程丽, 郭月, 龙卫华, 高建芹, 浦惠明, 张洁夫, 陈松. 油菜抗咪唑啉酮类除草剂基因标记的开发与应用[J]. 作物学报, 2020, 46(10): 1639-1646.
[5] 张平,姜一梅,曹鹏辉,张福鳞,伍洪铭,蔡梦颖,刘世家,田云录,江玲,万建民. 通过分子标记辅助选择将耐储藏主效QTL qSS-9 Kas转入宁粳4号提高其种子贮藏能力[J]. 作物学报, 2019, 45(3): 335-343.
[6] 杨勇,陆彦,郭淑青,石仲慧,赵杰,范晓磊,李钱峰,刘巧泉,张昌泉. 籼稻背景下导入Wx in等位基因改良稻米食味和理化品质[J]. 作物学报, 2019, 45(11): 1628-1637.
[7] 张安宁,刘毅,王飞名,谢岳文,孔德艳,聂元元,张分云,毕俊国,余新桥,刘国兰,罗利军. 节水抗旱稻恢复系的抗褐飞虱分子标记辅助选育及抗性评价[J]. 作物学报, 2019, 45(11): 1764-1769.
[8] 李玉玲,蒋正宁,胡文静,李东升,程婧晔,裔新,程晓明,吴荣林,程顺和. CIMMYT小麦种质C615抗叶锈病QTL分析[J]. 作物学报, 2018, 44(6): 836-843.
[9] 田宇,杨蕾,李英慧,邱丽娟. 抗大豆胞囊线虫SCN3-11位点的KASP标记开发和利用[J]. 作物学报, 2018, 44(11): 1600-1611.
[10] 朱展望, 徐登安, 程顺和, 高春保, 夏先春, 郝元峰, 何中虎. 中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源[J]. 作物学报, 2018, 44(04): 473-482.
[11] 张宏军, 宿振起, 柏贵华, 张旭, 马鸿翔, 李腾, 邓云, 买春艳, 于立强, 刘宏伟, 杨丽, 李洪杰, 周阳. 利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性[J]. 作物学报, 2018, 44(04): 505-511.
[12] 姚姝,陈涛,张亚东,朱镇,赵庆勇,周丽慧,赵凌,赵春芳,王才林. 利用分子标记辅助选择聚合水稻Pi-ta、Pi-b和Wx-mq基因[J]. 作物学报, 2017, 43(11): 1622-1631.
[13] 童治军,焦芳婵,方敦煌,陈学军,吴兴富,曾建敏,谢贺,张谊寒,肖炳光*. 烟草染色体片段代换系的构建与遗传评价[J]. 作物学报, 2016, 42(11): 1609-1619.
[14] 楼珏，杨文清，李仲惺，罗天宽，谢永楚，郑国楚，岳高红，徐建龙，卢华金. 聚合稻瘟病、白叶枯病和褐飞虱抗性基因的三系恢复系改良效果的评价[J]. 作物学报, 2016, 42(01): 31-42.
[15] 马建,马小定,赵志超,王帅,王久林,王洁,程治军，雷财林. 水稻抗稻瘟病基因Pi35功能性分子标记的开发及其应用[J]. 作物学报, 2015, 41(12): 1779-1790.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2