作物学报 ›› 2009, Vol. 35 ›› Issue (12): 2139-2149.doi: 10.3724/SP.J.1006.2009.02139
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
刘顺湖1,4,周瑞宝2,*,喻德跃1,陈受宜3,盖钧镒1,*
LIU Shun-Hu1,4,ZHOU Rui-Bao2,*, YU De-Yue1,CHEN Shou-Yi3,GAI Jun-Yi1,*
摘要:
以科丰1号×南农1138-2组合衍生的184个重组自交家系(简称RIKY)和(Essex×ZDD2315)×ZDD2315衍生的114个BC1F2家系(简称BIEX)为材料,对蛋白质含量、蛋油总量与油脂含量,11S、7S、11S/7S,11S-1~11S-4, 7S-1~7S-6等4组16个性状利用WinQTL Cartographer Ver.2.5的复合区间作图法(CIM)、多区间作图法(MIM)和IciMapping Ver.2.0的完备区间作图法(ICIM)进行QTL分析, 结果表明:(1) 在RIKY和BIEX群体分别定位到17+个和21+个QTL,合计38+个QTL;在RIKY有蛋白、油脂、蛋油总量QTL11个,在11S和7S亚基组上分别只有1+和3+个;在BIEX有前性状QTL2+个,有后性状QTL分别9+和6+个;(2) 两群体16个性状上均没有检测到共享的QTL,说明两群体的蛋白质有关性状具有完全不同的遗传基础;RIKY的两个亲本间蛋白、油脂和蛋油总量有明显遗传差异,但在亚基组上遗传差异不大,而BIEX则反之;(3) 4组总、分性状中,两群体一致表现出蛋白、油脂和蛋油总量和11S、7S和11S/7S比值两组在总、分性状间共享QTL(共同遗传基础), 而11S亚基组和7S亚基组两组性状在总、分性状间无共享的QTL;(4) 蛋白质有关性状QTL定位结果和分离分析结果共同表明这类性状主效基因和微效基因均占较大比重,要考虑两者兼用的育种方法。
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