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作物学报 ›› 2010, Vol. 36 ›› Issue (3): 376-384.doi: 10.3724/SP.J.1006.2010.00376

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

粳稻叶绿素含量QTL与其合成降解相关基因的比较分析

姜树坤1，张喜娟2，徐正进1,*，陈温福1   

  1. 1沈阳农业大学/农业部作物生理生态遗传育种重点开放实验室，辽宁沈阳110161；2辽宁省丹东农业科学院，辽宁凤城118109
  • 收稿日期:2009-10-15 修回日期:2009-12-08 出版日期:2010-03-12 网络出版日期:2010-01-22
  • 通讯作者: 徐正进, E-mail: xuzhengjin@126.com
  • 基金资助:

    本研究由国家自然科学基金项目（30871468）和国家重点基础研究发展计划（973计划）项目（2009CB126007）资助。

Comparison between QTLs for Chlorophyll Content and Genes Controlling Chlorophyll Biosynthesis and Degradation in Japonica Rice (Oryza sativa L.)

JIANG Shu-Kun1,ZHANG Xi-Juan2,XU Zheng-Jin1,*,CHEN Wen-Fu1   

  1. 1Key Laboratory of Crop Physiology，Ecology，Genetics and Breeding，Ministry of Agriculture/Shenyang Agricultural University，Shenyang 110161，China；2Dandong Academy of Agricultural Sciences，Fengcheng 118109，China
  • Received:2009-10-15 Revised:2009-12-08 Published:2010-03-12 Published online:2010-01-22
  • Contact: XU Zheng-JIn, E-mail: xuzhengjin@126.com

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1938

被引次数

34

摘要：

为剖析水稻叶绿素不同时期的表达规律及后期持绿的遗传机制，以沈农265和丽江新团黑谷的粳-粳交重组自交系为材料，对水稻分蘖期、抽穗期和成熟期的叶绿素含量以及生育后期的持绿能力进行QTL定位分析。检测到5个控制分蘖期叶绿素含量的QTL、7个控制水稻抽穗期叶绿素含量的QTL和10个控制成熟期叶绿素含量的QTL，分布在除第5染色体以外的11条染色体上。比较它们与编码叶绿素合成及降解过程中的重要酶的基因发现，虽然生育前期检测到的QTL较少，但对应的叶绿素合成降解相关基因却较多。随生育期的推移，检测到的QTL数目增多，但对应的叶绿素合成降解相关基因却减少。暗示生育前期大多数叶绿素合成(降解)相关基因表达的水平差异不大，后期控制叶绿素合成降解的个别关键基因表达水平增加。并以此为基础提出了叶片生育后期持绿的两种可能遗传基础。

关键词: 粳稻, 叶绿素含量, 持绿性, 数量性状位点, 叶绿素合成降解

Abstract:

To understand the expression patterns of chlorophyll content related genes at different stages and genetic mechanisms of stay-green at later stage, we analyzed the QTLs controlling chlorophyll content at the stages of tillering, heading and maturity by employing 126 recombinant inbred lines (RILs) derived from a cross between two japonica rice cultivars, Shennong 265 and Lijiangxintuanheigu. Five, seven and ten QTLs controlling chlorophyll contents at tillering stage, heading stage and maturity stage were detected, respectively. They were distributed on all rice chromosomes except chromosome 5. Comparison of the QTLs and the genes underlying the key enzymes of chlorophyll biosynthesis and degradation revealed that relatively more QTLs detected at earlier stage co-located with the genes related to chlorophyll biosynthesis and degradation. With the growth stage going on, more QTLs were detected but only a few of them involved in chlorophyll biosynthesis and degradation. The results suggested that the expression level of most genes related to chlorophyll biosynthesis (degradation) had no difference at earlier stage but specific key genes increased at later stage. And two possible genetic bases for stay-green were proposed.

Key words: Japonica rice, Chlorophyll content, Stay-green, Quantitative trait loci, Chlorophyll biosynthesis and degradation




[1] Beale S I. Green genes gleaned. Trends Plant Sci, 2005, 10: 309-312



[2] Hörtensteiner S. Chlorophyll degradation during senescence. Annu Rev Plant Biol, 2006, 57: 55-77



[3] Takahashi Y, Shomura A, Sasaki T, Yano M. Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the a subunit of protein kinase CK2. Proc Natl Acad Sci USA, 2001, 98: 7922-7927



[4] Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, Angeles E R, Qian Q, Kitano H, Matsuoka M. Cytokinin oxidase regulates rice grain production. Science, 2005, 309: 741-745



[5] Song X J, Huang W, Shi M, Zhu M Z, Lin H X. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat Genet, 2007, 39(5): 623-630



[6] Fan C C, Xing Y Z, Mao H L, Lu T T, Han B, Xu C G, Li X H, Zhang Q F. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative trans-membrane protein. Theor Appl Genet, 2006, 112: 1164-1171



[7] Jiang S-K (姜树坤), Zhong M(钟鸣), Xu Z-J(徐正进). Classification of rice cultivars with RAPD molecular markers. J Shenyang Agric Univ (沈阳农业大学学报), 2006, 37(4): 639-641 (in Chinese with English abstract)



[8] Panaud O, Chen X, Mccouch S D. Development of micro-satellite marker and characterization of sample sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597-607



[9] Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newberg L. MAPMAKER: an interactive computer for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174-182



[10] Zeng Z B. Precision mapping of quantitative trait loci. Genetics, 1994, 136: 1457-1468



[11] Huang C(黄成), Jiang S-K (姜树坤), Liu M-H(刘梦红), Xu Z-J(徐正进), Chen W-F(陈温福). QTL dissection of heading date in rice (Oryza sativa L. japanica). Acta Agric Boreali-Sin (华北农学报), 2009, 24(3): 7-9 ( in Chinese with English abstract)



[12] Cao S-Q(曹树青), Lu W(陆巍), Zhai H-Q(翟虎渠), Sheng S-L(盛生兰), Gong H-B(龚红兵), Yang T-N(杨图南), Zhang R-X(张荣铣). Research on the method to estimating flag leaf photosynthesis function duration at rice seedling stage by relative steady phase of chlorophyll content. Chin J Rice Sci (中国水稻科学), 2001, 15(4): 309-313 (in Chinese with English abstract)



[13] Liu W, Fu Y, Hu G, Si H, Zhu L, Wu C, Sun Z. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta,2007, 226: 785-795



[14] Zhang H, Li J, Yoo J H, Yoo S C, Cho S H, Koh H J, Seo H S, Paek N C. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 2006, 62: 325-337



[15] Jung K H, Hur J, Ryu C H, Choi Y, Chung Y Y, Miyao A, Hirochika H, An G. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44: 463-472



[16] Suzuki J Y, Bollivar D W, Bauer C E. Genetic analysis of chlorophyll biosynthesis. Annu Rev Genet, 1997, 31: 61-89



[17] Wu Z, Zhang X, He B, Diao L, Sheng S, Wang J, Guo X, Su N, Wang L, Jiang L, Wang C, Zhai H, Wan J. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29-40



[18] Morita R, Kusaba M, Yamaguchi H, Amano E, Miyao A, Hirochika H, Nishimura M. Characterization of chlorophyllide a oxygenase (CAO) in rice (Oryza sativa). Breed Sci, 55: 361-364



[19] Lee S, Kim J H, Yoo E S, Lee C H, Hirochika H, An G. Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 2005, 57: 805-818



[20] Kusaba M, Ito H, Morita R, Iida S, Sato Y, Fujimoto M, Kawasaki S, Tanaka R, Hirochika H, Nishimura M, Tanaka A. Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence. Plant Cell, 2007, 19: 1362-1375



[21] Jiang H, Li M, Liang N, Yan H, Wei Y, Xu X, Liu J, Xu Z, Chen F, Wu G. Molecular cloning and function analysis of the stay green gene in rice. Plant J, 2007, 52: 197-209



[22] Tong H-H(童汉华), Mei H-W(梅捍卫), Xing Y-Z(邢永忠), Cao Y-P(曹一平), Yu X-Q(余新桥), Zhang S-Q(章善庆), Luo L-J(罗利军). QTL analysis for morphological and physiological characteristics of flag leaf at the late developmental stage in rice. Chin J Rice Sci (中国水稻科学), 2007, 21(5): 493-499 (in Chinese with English abstract)



[23] Ishimaru K, Yano M, Aoki N, Ono K, Hirose T, Lin S Y, Monna L, Sasaki T, Ohsugi R. Toward the mapping of physiological and agronomic characters on a rice function map: QTL analysis and comparison between QTLs and expressed sequence tags. Theor Appl Genet, 2001, 102: 793-800

 

[24] Yang Q-H(杨权海), Lu W(陆巍), Hu M-L(胡茂龙), Wang C-M(王春明), Zhang R-X(张荣铣), Yano M(失野昌裕), Wan J-M(万建民). QTL and epistatic interaction underlying leaf chlorophyll and H2O2 content variation in rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2003, 30(3): 245-250



[25] Abdelkhalik A F, Shishido R, Nomura K, Ikehashi H. QTL-based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L.). Theor Appl Genet, 2005, 110: 1226-1235



[26] A Jia L-T(阿加拉铁), Zeng L-J(曾龙军), Xue D-W(薛大伟), Hu J(胡江), Zeng D-L(曾大力), Gao Z-Y(高振宇), Guo L-B(郭龙彪), Li S-G(李仕贵), Qian Q(钱前). QTL analysis for chlorophyll content in four grain-filling stage in rice. Acta Agron Sin (作物学报), 2008, 34(1): 61-66 (in Chinese with English abstract)

[27] Liu D-H(刘道宏). Plant leaf senescence. Plant Physiol Commun (植物生理学通讯), 1983, (2): 16-21 (in Chinese)
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