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作物学报 ›› 2010, Vol. 36 ›› Issue (4): 549-554.doi: 10.3724/SP.J.1006.2010.00549

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中品95-5117抗大豆花叶病毒基因源分析

关荣霞1，陈玉波1,2,**，方宏亮3，刘硕4，腾卫丽5，李文滨5，王丕武2，常汝镇1，邱丽娟1,*   

  1. 1 中国农业科学院作物研究所 / 国家农作物基因资源与遗传改良重大科学工程 / 农业部作物种质资源利用重点开放实验室，北京 100081; 2 吉林农业大学农学院，吉林长春 130118; 3 蚌埠医学院，安徽蚌埠 233030; 4 南昌大学，江西南昌330031; 5 东北农业大学大豆研究所，黑龙江哈尔滨 150030
  • 收稿日期:2009-08-27 修回日期:2009-12-08 出版日期:2010-04-12 网络出版日期:2010-01-22
  • 通讯作者: 邱丽娟, E-mail:qiu_lijuan@263.net
  • 基金资助:

    本研究由国家自然科学基金项目（30490251），国家高技术研究发展计划（863计划）项目（2006AA10Z1B3，2006AA10Z164，2006aa100104，2006AA10Z1F1），中国农业科学院作物科学研究所基金基本科研业务费专项（082060302-09）和国家科技攻关计划项目（2006BAD13B05）资助。

Origin Analysis of Resistance Gene to Soybean Mosaic Virus in Soybean Line ICGR95-5117

GUAN Rong-Xia1, CHEN Yu-Bo1,2,**, FANG Hong-Liang3, LIU Shuo4, TENG Wei-Li5, LI Wen-Bin5, WANG Pei-Wu2, CHANG Ru-Zhen1, and QIU Li-Juan1,*   

  1. 1 National Key Facility for Crop Gene Resource and Genetic Improvement / Key Laboratory of Germplasm Utilization (MOA), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Jilin Agricultural University, Changchun 130118, China; 3 Bengbu Medical College, Bengbu 233030, China; 4 Nanchang University, Nanchang 330031, China; 5 Soybean Research Institute, Northeast Agricultural University, Harbin 150030, China
  • Received:2009-08-27 Revised:2009-12-08 Published:2010-04-12 Published online:2010-01-22
  • Contact: QIU li-juan, E-mail:qiu_lijuan@263.net

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1381

被引次数

5

摘要：

中品95-5117和中品95-5383是以中品661为亲本选育的抗东北花叶病毒病3号株系(SMV3)的大豆新品系。中品95-5383抗性基因的SCAR标记已被定位于大豆F连锁群(Chr.13)，与抗病基因Rsv1紧密连锁。利用大豆F连锁群的34对SSR引物及与抗病基因紧密连锁的SCAR标记SCN11及Rsv1候选基因标记Rsv1-f/r，对中品95-5117系谱亲本进行检测，结合对SMV3的抗性鉴定结果进行分析，旨在明确抗SMV3基因在系谱中的传递规律，为利用分子标记辅助选择培育抗SMV3新品种提供依据。通过SSR标记分析发现，中品95-5117和中品95-5383与亲本中品661的相似性最高，而与另外一个亲本鲁豆4号关系较远。SCAR标记SCN11检测表明，只有1份材料Mangnolid(F-53)B为感病基因型。系谱的Rsv1-f/r标记分析表明，Williams 82是中品95-5117中Rsv1基因的供体亲本。抗病性鉴定发现鲁豆4号高抗SMV3，但它并不携带Rsv1基因。据上述结果推测中品95-5117中不仅含有Rsv1，还具有来自鲁豆4号的抗病基因，证明该品系比其亲本中品661具有对SMV3更强的抗性。

关键词: 大豆, 中品95-5117, 系谱, SMV

Abstract:

Soybean mosaic virus (SMV) is a prevalent viral pathogen of soybean. The soybean mosaic virus N3 (SMV3) is the most toxic strain in Northeast China. Soybean lines ICGR95-5117 and ICGR95-5383 with high resistance to SMV3, which were all derived from ICGR661, are recently released from Chinese Academy of Agricultural Sciences. Several genes resistant to soybean mosaic virus have been identified and mapped on different soybean linkage groups by molecular markers. The resistance gene in ICGR95-5383 has been mapped on soybean linkage group F near Rsv1 gene. A dominant marker developed from the candidate gene of Rsv1 has been reported recently. The purpose of this research was to find the origin of the SMV3 resistance gene in ICGR95-5117 and the relationship of this resistance gene with Rsv1 by analyzing the pedigree. Thirty four SSR markers selected from linkage group F and two markers, SCAR marker (SCN11) tightly linked with resistance gene in ICGR95-5383 and Rsv1-f/r makers co-segregated with resistance gene Rsv1, were used to detect the materials in pedigree of ICGR95-5117. The similarity coefficient of the two lines between ICGR95-5117, ICGR95-5383 and ICGR661 was the highest. All the material except for Mangnolid(F-53)B had SMV3 resistance allele at locus SCN11. Williams82 and ICGR661 had the same allelic variation at Rsv1-f/r locus with ICGR95-5117, revealing that the Rsv1 gene in ICGR95-5117 was inherited from Williams 82 via ICGR661. But ICGR95-5117 had higher resistance than ICGR661, indicating it might inherit different genes from SMV3 resistance parent Ludou4.

Key words: Soybean, ICGR95-5383, Pedigree, SMV

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