作物学报 ›› 2010, Vol. 36 ›› Issue (05): 713-720.doi: 10.3724/SP.J.1006.2010.00713
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
许凤华1,程治军1,王久林1,吴自明1,孙伟2,张欣1,雷财林1,王洁1,吴赴清1,郭秀平1,刘玲珑3,万建民1,3*
XU Feng-Hua1,CHENG Zhi-Jun1,WANG Jiu-Lin1,WU Zi-Ming1,SUN Wei2,ZHANG Xin1,WANG Jie1,WU Fu-Qing1,GUO Xiu-Ping1,LIU Ling-Long3,WAN Jian-Min1,3,*
摘要:
叶色突变是一类十分明显的性状突变,在高等植物的叶绿素合成、叶绿体结构、功能、遗传、分化与发育等基础研究中均具有重要意义。到目前为止,已鉴定多个重要的水稻功能基因,据不完全统计,水稻中至少已定位了79个叶色突变位点,并已成功克隆出多个叶色相关基因,其中OsCHLH、OsCAO1、OsCAO2、chlorina 1、chlorina 9、ygl等直接参与编码叶绿素合成,其余基因均参与叶绿体发育调控。在日本晴(Nipponbare) T-DNA插入突变体库中筛选到一例对温度敏感的白条纹突变体gws (green-white-stripe),遗传分析表明它来自组织培养过程中的单隐性基因突变。利用gws与培矮64杂交组合的F2代群体,将Gws精细定位于第六染色体标记InDel 15和InDel 16之间,物理距离为73 kb,此区间内包含13个基因。基因组序列分析发现,突变体在核糖核苷二磷酸还原酶小亚基(ribonucleoside-diphosphate reductase small chain, RNRS1)编码区第314~315碱基发生缺失,第316~317碱基由GC变为TT,导致该基因阅读框移码突变,蛋白质翻译提前终止。该基因是已经报道的水稻白条纹叶基因St1 (Stripe 1)的等位基因,gws突变体较st1突变体的白条纹出现早且明显,gws白条纹表型出现在第2片叶之后,而st1的白条纹表型仅出现在第四或五片叶之后。
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