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作物学报 ›› 2010, Vol. 36 ›› Issue (05): 713-720.doi: 10.3724/SP.J.1006.2010.00713

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

水稻白条纹叶Gws基因的精细定位与遗传分析

许凤华1,程治军1,王久林1,吴自明1,孙伟2,张欣1,雷财林1,王洁1,吴赴清1,郭秀平1,刘玲珑3,万建民1,3*   

  1. 1 中国农业科学院作物科学研究所, 北京100081；2 山东省临沂市农业科学院, 山东临沂276012；3 南京农业大学作物遗传与种质创新国家重点实验室 / 江苏省植物基因工程技术研究中心, 江苏南京210095
  • 收稿日期:2009-11-25 修回日期:2010-02-07 出版日期:2010-05-12 网络出版日期:2010-03-15
  • 通讯作者: 万建民, E-mail: wanjm@caas.net.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA100101）资助。

Genetic Analysis and Fine-Mapping of Gws Gene Using green-white-stripe Rice Mutant

XU Feng-Hua1,CHENG Zhi-Jun1,WANG Jiu-Lin1,WU Zi-Ming1,SUN Wei2,ZHANG Xin1,WANG Jie1,WU Fu-Qing1,GUO Xiu-Ping1,LIU Ling-Long3,WAN Jian-Min1,3,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Linyi Academy of Agricultural Sciences, Linyi 276012, China; 3 State Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-11-25 Revised:2010-02-07 Published:2010-05-12 Published online:2010-03-15
  • Contact: WAN Jian-min, E-mail: wanjm@caas.net.cn

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被引次数

34

摘要：

叶色突变是一类十分明显的性状突变，在高等植物的叶绿素合成、叶绿体结构、功能、遗传、分化与发育等基础研究中均具有重要意义。到目前为止，已鉴定多个重要的水稻功能基因，据不完全统计，水稻中至少已定位了79个叶色突变位点，并已成功克隆出多个叶色相关基因，其中OsCHLH、OsCAO1、OsCAO2、chlorina 1、chlorina 9、ygl等直接参与编码叶绿素合成,其余基因均参与叶绿体发育调控。在日本晴(Nipponbare) T-DNA插入突变体库中筛选到一例对温度敏感的白条纹突变体gws (green-white-stripe)，遗传分析表明它来自组织培养过程中的单隐性基因突变。利用gws与培矮64杂交组合的F2代群体，将Gws精细定位于第六染色体标记InDel 15和InDel 16之间，物理距离为73 kb，此区间内包含13个基因。基因组序列分析发现，突变体在核糖核苷二磷酸还原酶小亚基(ribonucleoside-diphosphate reductase small chain, RNRS1)编码区第314~315碱基发生缺失，第316~317碱基由GC变为TT，导致该基因阅读框移码突变，蛋白质翻译提前终止。该基因是已经报道的水稻白条纹叶基因St1 (Stripe 1)的等位基因，gws突变体较st1突变体的白条纹出现早且明显，gws白条纹表型出现在第2片叶之后，而st1的白条纹表型仅出现在第四或五片叶之后。

关键词: 水稻, 突变体, 叶绿素, 白条纹叶, stl突变体

Abstract:

Fine mapping and cloning of genes for leaf color mutation are of great importance in the study of chlorophyll biosynthesis and the structure, function, genetics and development of chloroplast in plant. A temperature-sensitive green-white-stripe leaf mutant gws was isolated from a rice T-DNA insertion mutant pool. Genetic analysis showed that the mutation was generated in tissue culture, rather than by T-DNA insertion, and it was controlled by a recessive nuclear gene. Linkage analysis of stripe plants with homologous recessive genes in an F2 population from the cross of gws × PA 64 indicated that Gws was flanked by the SSR markers of RM19680 and RM136 on Chromosome6, with genetic distances of 1.1 cM and 3.6 cM, respectively. The gene was further delimited by the newly developed markers InDel 15 and InDel 16 into a 73 kb region. A total of 13 genes, including 3 candidate leaf color genes, were found at this interval. Sequence alignments of these candidate genes between the wild-type and mutant revealed at least two bases deletion in the exon of LOC Os06g14620 (ribonucleoside-diphosphate reductase small chain), resulting in a frame-shift mutation and a premature stop codon. The same gene mutation caused by similar green-white-stripe (St1-Stripe 1) was documented previously. A slightly different phenotypes were observed between the mutants st1 and gws. In st1 plants, chlorotic leaves with a few longitudinal green stripes were observed until the fourth or fifth leaf blade mergence and the phenotype was later than that in gws emerged from second leaf seedling.

Key words: Rice, Mutant, Chloroplast, green-white-stripe, stl mutant

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