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作物学报 ›› 2010, Vol. 36 ›› Issue (06): 1044-1049.doi: 10.3724/SP.J.1006.2010.01044

• 研究简报 • 上一篇    下一篇

水分胁迫及复水条件下外源Ca2+对玉米幼苗根系水力导度及生长的影响

吴妍1,张岁岐1,2,*,刘小芳2,山仑1,2   

  1. 1 西北农林科技大学 / 黄土高原土壤侵蚀与旱地农业国家重点实验室，陕西杨凌 712100；2 中国科学院水利部水土保持研究所，陕西杨凌 712100
  • 收稿日期:2009-12-15 修回日期:2010-03-19 出版日期:2010-06-12 网络出版日期:2010-04-14
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2009CB118604），国家高技术研究发展计划（863计划）项目（2007AA100202），国家自然科学基金项目（30971714）和教育部新世纪优秀人才支持计划项目资助。

Effect of Calcium on Maize Seedling Root Hydraulic Conductivity and Growth under Water Stress and Rehydration Conditions

 WU Yan1,ZHANG Sui-Qi1,2,*,LIU Xiao-Fang2,SHAN Lun1,2   

  1. 1State Key Laboratory of Soil Erosion and Dryland farming of the Loess Plateau/Northwest A&F University,Yangling 712100,China;2Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry of Water Resources,Yangling 712100,China
  • Received:2009-12-15 Revised:2010-03-19 Published:2010-06-12 Published online:2010-04-14

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1571

被引次数

8

摘要：

利用10%PEG-6000模拟–0.2 MPa的水分胁迫，研究了外源Ca2+(在1/2 Hoagland营养液中添加10 mmol L-1 CaCl2)对水分胁迫7 d后及复水2 d，玉米幼苗整株根系水力导度(Lpr)、根系生长及叶水势(ψw)的影响。结果表明，正常水分条件下，外源Ca2+处理降低了Lpr，但对叶水势无影响；水分胁迫条件下，外源Ca2+显著提高了Lpr、叶水势，减缓了水分胁迫对植物的伤害；复水1 d，两种钙水平下Lp均无明显恢复，但Ca2+处理的Lpr显著高于对照，而叶水势无显著差异且均能恢复至正常供水时的水平；复水2 d，Ca2+处理的Lpr即能恢复至正常供水时的水平，对照仅恢复为正常供水时的59.06%。进一步用HgCl2检测表明，正常水分条件下外源Ca2+对水通道蛋白(AQP)活性没有影响；而水分胁迫下，外源Ca2+提高了AQP活性，对照AQP活性下降，说明水分胁迫时外源Ca2+促进了水分跨膜途径运输；复水2 d，外源Ca2+处理AQP活性恢复至正常供水时的水平，对照AQP活性未能恢复。另外，外源Ca2+处理减缓了水分胁迫对植物生长发育的抑制作用，促进了复水时侧根发育，增加根系吸水面积，为植株迅速恢复供水提供了形态学基础，增加了复水后的补偿效应。

关键词: 水分胁迫, 复水, Ca2+, AQP, 根系水力导度

Abstract:

The effects of extra calcium (additions of 10 mmol L-1 CaCl2 into half-strength Hoagland solution) on maize (Zea mays L.) seedling root hydraulic conductivity (Lpr), morphology and leaf water potential (ψw) were investigated under water stress, which was simulated by 10% PEG-6000 with osmotic potential (ψs) value of -0.2 MPa for seven days, and subsequent two days rehydration. Whole root hydraulic conductivity (Lpr) and leaf water potential (ψw) were determined by pressure chamber. After these treatments, it could be seen that water stress reduced Lpr, which was restored when calcium was added to the solution for growing the water-stressed plants. In addition, the Lpr recovered to the level of the control after re-watering at high Ca2+ level, but the recovery of Lpr was only 59.06% at regular Ca2+ level. HgCl2 (50 μmol L-1) treatment caused a sharp decline in Lpr, which was almost restored by treatment with 5 mmol L-1 β-mercaptoethanol. The reduction of Lpr by Hg2+ was 53.20% and 74.55% at regular and high calcium levels, respectively under water stress condition, and 62.1% under normal condition. The results suggest that Ca2+ increased the passage of water through the cell membrane of roots by increasing the activity of Hg-sensitive AQP under water stress. The percentage of reduction by Hg2+ was decreased to the control level two days after re-watering in CaCl2 treated plants. The leaf water potential was declined significantly under water stress, particularly at regular calcium level with respect to the decrease of Lpr. However, ψw recovered rapidly onw day after re-watering. Furthermore, water stress had a detrimental effect on the root growth. The addition of calcium, especially at low water potential, increased the root surface area and primary root diameter, and it promoted primary root enlongation and lateral root development. Compared with the controls, the growth of Ca treated plants was recovered gradually with the prolonging of rehydration. Therefore, these results indicated that the extra calcium, with respect to root growth and water uptake, mitigates the negative effect of water stress and enhances the compensatory effects of rehydration.

Key words: Water stress, Rehydration, Calcium, AQP, Root hydraulic conductivity

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