作物学报 ›› 2011, Vol. 37 ›› Issue (07): 1259-1265.doi: 10.3724/SP.J.1006.2011.01259
朱娟娟1,梁银丽1,2,Tremblay Nicolas3,*
ZHU Juan-Juan1,LIANG Yin-Li1,2,Tremblay Nicolas3,*
摘要: SPAD-502叶绿素仪与Dualex-3多酚仪均可诊断作物的氮素营养状况, 为了探讨不同水分条件下该指标对作物施氮量的响应, 以玉米品种Pioneer 38B84为研究对象, 在温室盆栽条件下, 研究3个土壤水分水平(干旱、干旱后复水和水分良好)和4个施氮水平(0、50、50+75和200 kg hm-2), 即12个处理对植物含氮量、SPAD值、Dualex值以及SPAD/Dualex比值的影响。结果表明, 植物含氮量、SPAD值和SPAD/Dualex比值均随施氮量的增加而增加, Dualex值随施氮量的增加而减少。干旱胁迫初期, 植物含氮量、SPAD值和SPAD/Dualex比值迅速下降, Dualex值迅速增加;随着干旱胁迫时间的推进, 各指标逐渐呈相反趋势变化。干旱复水后, 植物含氮量、Dualex值和SPAD/Dualex比值逐渐恢复, 然而SPAD值恢复较小。SPAD值(r=0.92)、Dualex值(r= -0.86)及SPAD/Dualex(r=0.63)值与植物含氮量呈极显著的相关性, 但试验后期, 由于生长阶段的不同, SPAD值和Dualex值与植物含氮量在干旱条件下(SPAD:r=0.90;Dualex:r= -0.83)的相关性高于在水分良好条件下(SPAD:r=0.39; Dualex:r= -0.44)。通过比较不同水分条件下, SPAD值、Dualex值和SPAD/Dualex值对不同施氮量的响应, 发现Dualex值在不同水分条件下均能较好地反应施氮水平。
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