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作物学报 ›› 2010, Vol. 36 ›› Issue (07): 1061-1066.doi: 10.3724/SP.J.1006.2010.01061

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

大豆质核互作雄性不育系NJCMS3A双亲雄性育性基因的SSR标记

李曙光,赵团结*,盖钧镒*   

  1. 南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,江苏南京210095
  • 收稿日期:2010-01-18 修回日期:2010-04-20 出版日期:2010-07-12 网络出版日期:2010-05-20
  • 通讯作者: 赵团结, 盖钧镒, E-mail: sri@njau.edu.cn; Tel: 025-84395405
  • 基金资助:
    本研究由国家高技术研究发展计划(863计划)项目(2009AA101106, 2006AA100104), 国家重点基础研究发展计划(973计划)项目(2010CB125906, 2009CB118404, 2006CB101708), 国家自然科学基金项目(30671314)和高等学校创新引智计划(B08025)资助。

Mapping Male Fertility Gene with SSR Markers in Parents of Cytoplasmic- Nuclear Male-Sterile Line NJCMS3A in Soybean

LI Shu-Guang,ZHAO Tuan-Jie*,GAI Jun-Yi*   

  1. Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095,China
  • Received:2010-01-18 Revised:2010-04-20 Published:2010-07-12 Published online:2010-05-20
  • Contact: ZHAO Tuan-Jie,GAI Jun-Yi,E-mail: sri@njau.edu.cn; Tel: 025-84395405

摘要: 利用大豆质核互作雄性不育系NJMCS3A的质、核供体亲本N21566和N21249构建F2和BC1F1育性分离群体进行雄性育性的遗传分析与基因定位。结果表明, F1正反交可育,F2和BC1F1的可育株与不育株分离比例经χ2测验分别符合3∶1和1∶1,表明NJCMS3A供体亲本雄性育性由一对基因控制,可育等位基因为显性。该基因可能是NJCMS3A的一个恢复基因。选用793对SSR引物对F2和BC1F1群体分别进行育性基因定位,发现该育性基因位于O连锁群上,在Satt331和Satt477标记之间,与Satt331、CSSR133和Satt477标记距离的次序一致,分别为8.1~10.4 cM、11.4~16.4 cM、13.3~19.2 cM。

关键词: 大豆, 质核互作雄性不育, 雄性育性遗传, 基因定位

Abstract: Utilization of the three lines (cytoplasmic-nuclear male-sterile (CMS) line, maintainer line and restorer line) in producing hybrids with heterosis has been very successful in a number of crops including soybean. The soybean CMS line NJCMS3A was developed through six consecutive backcross generations with N21566 as cytoplasm source and N21249 as nuclear donor. The objective of our study was to reveal the genetic mechanism and mapping the male fertility gene in the two parents of the CMS line NJCMS3A, i.e. N21566 and N21249. The male fertility of (N21566×N21249) F2 and BC1F1 showed that the segregation ratio of fertile to sterile plants fitted the expected ratios of 3:1 and 1:1, respectively, indicating one pair of gene conferring male fertility in the parents of NJCMS3A with dominance allele in N21249 being male fertile. This gene might be one of the restorer genes for the male sterility of NJCMS3A. The mapping results on BC1F1 and F2 showed that out of the 793 randomly selected SSR markers, Satt331, CSSR133 and Satt477 on linkage group O were linked to the male fertility gene Rf with their genetic distances of 8.1~10.4 cM, 11.4~16.4 cM, 13.3~19.2 cM, respectively, in a same order and its location between Satt331 and Satt477.

Key words: Soybean, Cytoplasmic-nuclear male sterility, Fertility inheritance, Gene mapping

[1]         Bentolila S, Alfonso A A, Hanson M R. A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants. Proc Natl Acad Sci USA, 2002, 99: 10887–10892
[2]         Schnable P S, Wise R P. The molecular basis of cytoplasmic male sterility and fertility restoration. Trends Plant Sci, 1998, 3: 175–180
[3]         Wise R P, Pring D R. Nuclear-mediated mitochondrial gene regulation and male fertility in higher plants: Light at the end of the tunnel? Proc Natl Acad Sci USA, 2002, 99: 10240–10242
[4]         Davis W H. Route to hybrid soybean production. United States Patent. 1985, US 4545146
[5]         Sun H(孙寰), Zhao L-M(赵丽梅), Huang M(黄梅). Studies on cytoplasmic-nuclear male sterile soybean. Chin Sci Bull, 1993, 38: 1535–1536 (in Chinese)
[6]         Zhao L-M(赵丽梅), Sun H(孙寰), Huang M(黄梅). The development and preliminary studies on cytoplasmic male sterile soybean line ZA. Soybean Sci(大豆科学), 1998, 17(3): 268–270 (in Chinese with English abstract)
[7]         Gai J Y, Cui Z L, Ji D F, Ren Z J, Ding D R. A report on the nuclear cytoplasmic male sterility from a cross between two soybean cultivars. Soybean Genet Newslett, 1995, 22: 55–58
[8]         Ding D R, Gai J Y, Cui Z L, Qiu J X. Development of a cytoplasmic-nuclear male-sterile line of soybean. Euphytica, 2002, 124: 85–91
[9]         Bai Y-N(白羊年), Gai J-Y(盖钧镒). Development of soybean cytoplasmic-nuclear male-sterile line NJCMS2A and restorability of its male fertility. Sci Agric Sin (中国农业科学), 2003, 36(7): 740–745 (in Chinese with English abstract)
[10]      Zhao T J, Gai J Y. Discovery of new male-sterile cytoplasm sources and development of a new cytoplasmic-nuclear male-sterile line NJCMS3A in soybean. Euphytica, 2006, 152: 387–396
[11]      Zhang L(张磊), Dai O-H(戴瓯和). Selection and breeding of nucleo-cytoplasmic male sterile line W931A in soybean. Sci Agric Sin (中国农业科学), 1997, 30(6): 90–91 (in Chinese with English abstract)
[12]      Zhang L(张磊), Dai O-H(戴瓯和), Zhang L-Y(张丽亚). Breeding of soybean male sterile line of nucleo-cyotplasmic interaction. Soybean Sci (大豆科学), 1999, 18(4): 327–330 (in Chinese with English abstract)
[13]      Zhang L(张磊), Dai O-H(戴瓯和), Huang Z-P(黄志平), Li J-Q(李杰坤). Selection of soybean male sterile line of nucleo-cytoplasmic interaction and its fertility. Sci Agric Sin (中国农业科学), 1999, 32(4): 34–38 (in Chinese with English abstract)
[14]      Xu Z-Y(许占友), Li L(李磊), Chang R-Z(常汝镇), Qiu L-J(邱丽娟), Wang M-B(汪茂斌), Li Z(李智), Yu W(于伟), Li X-H(李向华). Genetic analysis of nuclear male sterile genes of soybean cytoplasmic male sterile lines. Sci Agric Sin(中国农业科学), 1999, (suppl): 1–8 (in Chinese with English abstract)
[15]      Zhao L-M(赵丽梅), Sun H(孙寰), Wang S-M(王曙明), Wang Y-Q(王跃强), Huang M(黄梅), Li J-P(李建平). Breeding of hybrid soybean HybSoy 1. Chin J Oil Crop Sci (中国油料作物学报), 2004, 26(3): 15–17 (in Chinese with English abstract)
[16]      Peng B(彭宝), Zhao L-M(赵丽梅), Wang S-M(王曙明), Cheng Y-X(程砚喜), Sun H(孙寰), Wang Y-Q(王跃强), Li J-P(李建平), Zhang W-L(张伟龙). Studies on breeding of “HybSoy 2” soybean and high yield. J Jilin Agric Sci (吉林农业科学), 2008, 33(2): 3–4 (in Chinese with English abstract)
[17]      Zhang L(张磊), Dai O-H(戴瓯和), Huang Z-P(黄志平), Li J-Q(李杰坤), Zhang L-Y(张丽亚), Hu C(胡晨). Breeding of hybrid soybean Zayoudou 1. Soybean Bull (大豆通报), 2007, (2): 14–16 (in Chinese)
[18]      Xu Z-Y(许占友), Li L(李磊), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Wang M-B(汪茂斌), Li Z(李智), Guo B(郭蓓). Selection of three lines and localization of the restorer genes in soybean using SSR markers. Sci Agric Sin (中国农业科学), 1999, 32(2): 32–38 (in Chinese with English abstract)
[19]      Zhao L-M(赵丽梅), Wang Y-M(王玉民), Sun H(孙寰), Zhao H-K(赵洪锟), Cheng Y-X(程延喜), Peng B(彭宝), Wang S-M(王曙明), Zhang W-L(张伟龙), Dong Y-S(董英山). Identification of SSR markers linked to the fertility restorer gene for the CMS in soybean. Soybean Sci (大豆科学), 2007, 26(6): 835–939 (in Chinese with English abstract)
[20]      Wang Y, Zhao L, Wang X, Sun H. Molecular mapping of a fertility restorer gene for cytoplasmic male sterility in soybean. Plant Breed, 2010, 129: 9–12
[21]      Yang S P, Duan M P, Meng Q C, Qiu J, Fan J M, Zhao T J, Yu D Y, Gai J Y. Inheritance and gene tagging of male fertility of cytoplasmic-nuclear male-sterile line NJCMS1A in soybean. Plant Breed, 2007, 126: 302–305
[22]      Dong J-S(董建生), Yang S-P(杨守萍), Yu D-Y(喻德跃), Gai J-Y(盖钧镒). Inheritance and gene tagging of male fertility restoration of cytoplasmic-nuclear male sterile line NJCMS2A in soybean. Soybean Sci (大豆科学), 2008, 27(2): 181–185 (in Chinese with English abstract)
[23]      Tang F-Y(汤复跃), Zhou L-R(周立人), Cheng X(程潇), Zhang L(张磊), Chen P(陈培), Jiang Y-F(江莹芬). SSR marker location for fertility restorer gene of M-cytoplasmic male sterility in soybean. Soybean Sci (大豆科学), 2008, 27(3): 383–386 (in Chinese with English abstract)
[24]      Keim P, Olson T C, Shoemaker R C. A rapid protocol for isolating soybean DNA. Soybean Genet Newslett, 1988, 15: 150–152
[25]      Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregate analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[26]      Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122–128
[27]      Xia Z, Tsubokura Y, Hoshi M, Hanawa M, Yano C, Okamura K, Ahmed T A, Anai T, Watanabe S, Hayashi M, Kawai T, Hossain K G, Masaki H, Asai K, Yamanaka N, Kubo N, Kadowaki K, Nagamura Y, Yano M, Sasaki T, Harada K. An integrated high-density linkage map of soybean with RFLP, SSR, STS, and AFLP markers using a single F2 population. DNA Res, 2007, 14: 257–269 Ding D-R(丁德荣), Gai J-Y(盖钧镒). Pollinating insects and natural outcrossing amount of soybean male sterile materials in southern china. Soybean Sci (大豆科学), 2000, 19(1):74–79 (in Chinese with English abstract)
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