欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2011, Vol. 37 ›› Issue (12): 2293-2298.doi: 10.3724/SP.J.1006.2011.02293

• 研究简报 • 上一篇    下一篇

苗期低温胁迫对扬麦16叶片抗氧化酶和渗透调节物质的影响

李春燕,陈思思,徐雯,李东升,顾骁,朱新开,郭文善,封超年*   

  1. 扬州大学江苏省作物遗传生理重点实验室, 江苏扬州 225009
  • 收稿日期:2011-05-27 修回日期:2011-09-18 出版日期:2011-12-12 网络出版日期:2011-09-29
  • 通讯作者: 封超年, E-mail: fengchaonian@yzu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(0971729, 31000683), 江苏省高校自然科学研究项目(10KJB210007)和江苏省科技支撑计划项目(BE2009426)资助。

Effect of Low Temperature at Seedling Stage on Antioxidation Enzymes and Cytoplasmic Osmoticum of Leaves in Wheat Cultivar Yangmai 16

LI Chun-Yan,CHEN Si-Si,XU Wen,LI Dong-Sheng,GU Xiao,ZHU Xin-Kai,GUO Wen-Shan,FENG Chao-Nian*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China
  • Received:2011-05-27 Revised:2011-09-18 Published:2011-12-12 Published online:2011-09-29
  • Contact: 封超年, E-mail: fengchaonian@yzu.edu.cn

PDF

1901

被引次数

83

摘要： 以扬麦16为试验材料, 利用人工气候箱模拟低温逆境, 研究-4℃、-6℃和-8℃低温胁迫对小麦苗期生长和叶片生理特性的影响。结果表明, 随着处理温度的降低、胁迫时间的延长, 小麦植株由叶尖萎蔫变黄的2、3级冻害加重至叶片全枯的4级冻害, 甚至大部分茎蘖冻死的5级冻害, –4℃和–6℃处理24 h没有发生4级及以上冻害。–8℃处理24 h和48 h后植株5级冻害比例显著增加, 植株死亡率分别为16.7%和39.6%。低温胁迫初期小麦叶片中SOD、POD和CAT活性均呈上升趋势, 随胁迫程度加重, SOD最先表现出下降趋势, POD和CAT变化稍缓, MDA含量增加。苗期低温胁迫使叶片中游离脯氨酸、可溶性蛋白和可溶性糖含量明显高于对照, 且随温度的降低和处理时间的延长呈上升趋势, 表现出对低温逆境的适应性。

关键词: 小麦, 苗期, 低温, 生理特性

Abstract: Morphological, physiological and biochemical changes in wheat seedlings (cv. Yangmai 16) under low temperature treatments (-4°C, -6°C, and -8°C) were studied by means of artificial temperature-controlled phytotron. Severer freezing-injured plants were observed in treatments with lower temperature and longer stress. The symptoms of injury were that the top of leaves became yellow and wilting (the second and third grade of freezing injury), then the whole leaves were all wilting (the fourth grade of freezing injury), and main stems and most of tillers were finally dead (the fifth grade of freezing injury). No plants showed freezing injury greater than the third grade in treatments of -4°C and -6°C for 24 h. However, the treatments of -8°C for 24 h and 48 h resulted in 16.7% and 39.6% of plant being frozen to death, respectively. The activities of superoxide dismutase (SOD), catalase (CAT), and peroxide dismutase (POD) in the second leaf from the top increased at the beginning of low temperature treatments, and with the increasing of stressSOD activity declined. The contents of malonaldehyde (MDA), proline, soluble sugar, and soluble protein were significantly increased in treatments with lower temperature and longer stress. These physiological and biochemical changes may help to alleviate the low-temperature injury to wheat plants.

Key words: Wheat, Seedling stage, Low temperature, Physiological characteristics

[1]Li M-S(李茂松), Wang D-L(王道龙), Zhong X-L(钟秀丽), Wang C-Y(王春艳), Shu C-H(苏常红), Zhao P(赵鹏), Yan X-Y(阎旭雨), Kiribuchi-Otobe C, Yoshida H. Current situation and prospect of research on frost of winter wheat. J Nat Disasters (自然灾害学报), 2005, 14(4): 72–78 (in Chinese with English abstract)
[2]Sun Y-D (孙亚东), Yang S-Y (杨书运). Analysis on the effects of low temperature and snow disaster on overwintering crops in 2008. J Anhui Agric Sci (安徽农业科学), 2008, 36(14): 5826–5827 (in Chinese with English abstract)
[3]Yu J(于晶), Zhang L(张林), Cui H(崔红), Zhang Y-X(张永侠), Cang J(苍晶), Hao Z-S(郝再彬), Li Z-F(李卓夫). Physiological and biochemical characteristics of Dongnongdongmai 1 before wintering in high-cold area. Acta Agron Sin (作物学报), 2008, 34(11): 2019–2025 (in Chinese with English abstract)
[4]Wang J-Y(王纪元), Yu S-Q(喻尚其). Studies on resistance and membrane lipid fluidity of various breeds of cold-resistant wheat. Biotechnology (生物技术), 1998, 8(2): 28–30 (in Chinese with English abstract)
[5]Zhao J-Z(赵晋忠), Shi R-X(史瑞雪), Ding C-S(丁超盛). The study on relationship between radicals metabolize and cold injury and resistance in winter wheat. J Shanxi Agric Univ (山西农业大学学报), 2001, 21(1): 57–59 (in Chinese with English abstract)
[6]Yu M(喻敏), Hu C-X(胡承孝), Wang Y-H(王运华). Influence of molybdenumon the activity of catalase and peroxidase in winter wheat cultivars with different molybdenum efficiency. Plant Nutr Fert Sci (植物营养与肥料学报), 2007, 13(1): 164–166 (in Chinese with English abstract)
[7]Wang S-G(王树刚), Wang Z-L(王振林), Wang P(王平), Wang H-W(王海伟), Li F(李府), Huang W(黄玮), Wu Y-G(武玉国), Yin Y-P(尹燕枰). Evaluation of wheat freezing resistance based on the responses of the physiological indices to low temperature stress. Acta Ecol Sin (生态学报), 2011, 31(4): 1064–1072 (in Chinese with English abstract)
[8]Sun J-Y(孙金月), Zhao Y-T(赵玉田), Liang B-W(梁博文), Liu F(刘方), You Y(尤勇). Changes of hydroxyproline-rich glycoprotein in wheat under cold stress and its relationship to cold resistance. J Plant Genet Resour (植物遗传资源学报), 2004, 5(1): 6–11 (in Chinese with English abstract)
[9]Wang H-Z(王贺正), Chen M-C(陈明灿), He W-C(贺文闯), Liu Y-J(李友军), Fu G-Z(付国占), Xiu G-W(徐国伟). Effect of phosphorus and potassium on cold resistance of wheat seedling. J Triticeae Crops (麦类作物学报), 2009, 29(1): 141–145 (in Chinese with English abstract)
[10]Gu D-J(顾德军), Liu R-K(刘荣坤). The effects of potassium dihydrogen phosphate buffer on the superoxid dismutase and cold-resistance of wheat seedlings. Agronomy Abroad: Wheat, Barley, Triticale, etc. (国外农学——麦类作物), 1994, 14(6): 48–49 (in Chinese with English abstract)
[11]Zhang Z-L(张志良). Handbook of Plant Physiology Experiment (植物生理学实验指导). Beijing: Higher Education Press, 1990 (in Chinese)
[12]Wang X-N(王晓楠), Fu L-S(付连双), Li Z-F(李卓夫), Sun Y-L(孙艳丽), Wang Y-B(王玉波), Liu C(刘灿), Wang J-W(王金伟), Chen Y-X(陈禹兴). Morphogenesis and physiological basis in wheat cultivars with different levels of cold-resistance during cold acclimation and freezing period. Acta Agron Sin (作物学报), 2009, 35(7): 1313–1319 (in Chinese with English abstract)
[13]Wang X-X(王孝宣), Li S-D(李树德), Dong H-R(东惠茹), Gao Z-H(高振华), Dai S-S(戴善书). The relationship of cold-tolerance with ABA, soluble sugar and respiratory intensity in tomato. Acta Hort Sin (园艺学报), 1998, 25(1): 56–60 (in Chinese with English abstract)
[14]Liu H-Y(刘慧英), Zhu Z-J(朱祝军), Lü G-H(吕国华), Qian Q(钱秋). Study on relationship between physiological changes and chilling tolerance in grafted watermelon seedlings under low temperature stress. Sci Agric Sin (中国农业科学), 2003, 36(1): 1325–1329 (in Chinese with English abstract)
[15]Chen J-Z(陈杰忠), Xu C-X(徐春香), Liang L-F(梁立峰). Effect of low temperature on protein and proline in banana (Musa spp.) leaves. J South China Agric Univ (华南农业大学学报), 1999, 20(3): 54–58 (in Chinese with English abstract)
[1] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[2] 胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846.
[3] 陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875.
[4] 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[5] 习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617.
[6] 高沛阳, 李瑾璇, 董宇奎, 石玉, 张振, 张永丽. 测墒补灌下小麦分蘖发生和成穗对施氮量的响应[J]. 作物学报, 2026, 52(6): 1847-1858.
[7] 金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829.
[8] 张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[9] 徐苗苗, 邸太妹, 王洁, 吴叶蝶, 刘恩贝, 王玉春, 王新超, 王璐. 外源槲皮素增强茶树抗寒性的分子机制[J]. 作物学报, 2026, 52(5): 1418-1429.
[10] 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[11] 姚术, 郭凯悦, 翟慧慧, 姚佳慧, 邓文琪, 闫玲, 黄驰, 高阳, 俞嫣然, 赵振邦, 李英慧, 王晓波, 李佳佳. 大豆苗期耐低铁综合评价及优异种质筛选[J]. 作物学报, 2026, 52(5): 1373-1387.
[12] 何万龙, 耿洪伟, 张飞飞, 米克热阿依·阿巴白克热, 罗紫洋, 李鹏程, 周钊宇, 程宇坤. 基于深度学习的小麦重要病害图像识别系统的研究[J]. 作物学报, 2026, 52(5): 1401-1417.
[13] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[14] 侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219.
[15] 尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2