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作物学报 ›› 2012, Vol. 38 ›› Issue (04): 732-739.doi: 10.3724/SP.J.1006.2012.00732

• 耕作栽培·生理生化 • 上一篇    下一篇

低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响

孙富1,3，杨丽涛1,*，谢晓娜1，刘光玲1，李杨瑞1,2,3,*   

  1. 1 广西大学农学院 / 亚热带农业生物资源保护与利用国家重点实验室，广西南宁530005；2 广西农业科学院，广西南宁530007；3中国农业科学院甘蔗研究中心 / 广西作物遗传改良生物技术重点开放实验室 / 广西甘蔗遗传改良重点实验室，广西南宁530007
  • 收稿日期:2011-07-27 修回日期:2011-12-19 出版日期:2012-04-12 网络出版日期:2012-02-13
  • 通讯作者: 杨丽涛, E-mail: litao61@hotmail.com; 李杨瑞, E-mail: liyr@gxaas.net
  • 基金资助:

    本研究由国家科技支撑计划项目(2007BAD30B00，2008BADB8B00)，科技部国际合作项目(2008DFA30600，2009DFA30820)，引进国外先进农业科学技术计划(948计划)项目(2009-Z8)，广西科技攻关重点项目(桂科攻0782004，桂科攻0895003-2，桂科攻0992021-2)和广西科技攻关项目(桂科能0815011)资助。

Effect of Chilling Stress on Physiological Metabolism in Chloroplasts of Seedlings of Sugarcane Varieties with Different Chilling Resistance

SUN Fu1,3,YANG Li-Tao1,*,XIE Xiao-Na1,LIU Guang-Ling1,LI Yang-Rui1,2,3,*   

  1. 1 Agricultural College / State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530005, China; 2 Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3 Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Crop Genetic Improvement and Biotechnology Lab / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China.
  • Received:2011-07-27 Revised:2011-12-19 Published:2012-04-12 Published online:2012-02-13
  • Contact: 杨丽涛, E-mail: litao61@hotmail.com; 李杨瑞, E-mail: liyr@gxaas.net

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被引次数

79

摘要： 土培桶栽抗寒品种GT28与不抗寒品种ROC22，测定正常(28℃)和低温(4℃)条件下甘蔗幼苗叶片的质膜透性、叶绿素荧光参数等相关生理指标。结果表明，低温与正常条件下相比，两品种幼苗叶片的膜透性显著增大，且ROC22增幅较大；初始荧光(Fo)、非光化学猝灭系数(NPQ)显著上升，最大光能转化效率(Fv/Fm)、反应中心激发能捕获效率(Fv'/Fm')、光化学猝灭系数(qP)、PSII实际量子效率(ΦPSII)均下降，以ROC22降幅较大；叶绿体希尔反应活力、Mg2+-ATPase、Ca2+-ATPase活性下降，叶绿体中丙二醛(MDA)含量增加，以ROC22变幅较大；超氧化物歧化酶(SOD)、过氧化物酶(POD)、谷胱甘肽还原酶(GR)活性均上升，GT28的增幅比ROC22显著。上述参数与品种抗寒性密切相关，GT28比ROC22抗寒性更强。

关键词: 甘蔗, 低温胁迫, 叶绿体, 叶绿素荧光, ATP酶, 活性氧

Abstract: Chilling occurs frequently in recent years, and has become a major limiting factor for sugarcane production. Chloroplasts are the main organelle for photosynthesis and one of the subcellular fractions for chilling sensitivity in plants. In order to investigate the effect of chilling stress on the physiological metabolism of sugarcane chloroplast, and reveal the relationships between the physiological characteristics in chloroplast and chilling tolerance of sugarcane, the experiment was conducted with two varieties of GT28 (chilling resistant) and ROC22 (chilling susceptible) with two treatments: control (28°C) and chilling stress (4°C). The relative electrical conductance (REC) of sugarcane seedling leaves, and chlorophyll fluorescence parameters and some physiological indiees in chloroplasts were measured. The results showed that under the low temperature stress condition, the plasma membrane permeability of the seedling leaves, initial fluorescence (Fo) and NPQ increased significantly in both varieties, and the chilling resistant variety GT28 was more stable than the chilling sensitive variety ROC22; and theFv/Fm, Fv'/Fm', qP, and ΦPSII decreased in the two sugarcane varieties, and decreased less in GT28 than in ROC22. The activities of Hill reaction, Mg2+-ATPase and Ca2+-ATPase in chloroplasts decreased, and those in GT28 decreased less than those in ROC22. The MDA content and SOD, POD, GR activities in the chloroplasts increased, and sugarcane variety GT28 showed lower increase in MDA but higher increase in SOD, POD, GR activities. The chlorophyll fluorescence parameters and the physiological characteristics in the chloroplasts of sugarcane leaves were affected significantly by chilling stress. The changes of chlorophyll fluorescence and the physiological characteristics in the chloroplasts are correlated significantly to low temperature resistance, and GT28 is more chilling resistant than ROC22.

Key words: Sugarcane (Saccharum officenarum L), Chilling stress, Chloroplast, Chlorophyll fluorescence, ATPase, ROS

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