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作物学报 ›› 2012, Vol. 38 ›› Issue (08): 1387-1396.doi: 10.3724/SP.J.1006.2012.01387

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

贵州省花生地方品种的遗传多样性

林茂1,2，李正强2，郑治洪2，吕建伟2，马天进2，李超3,*，阚健全1,*   

  1. 1西南大学食品科学学院，重庆400716；2贵州省农作物品种资源研究所，贵州贵阳550006; 3贵州省油料研究所，贵州贵阳550006
  • 收稿日期:2011-12-16 修回日期:2012-04-15 出版日期:2012-08-12 网络出版日期:2012-05-11
  • 通讯作者: 阚建全, E-mail:ganjq1965@163.com, Tel:023-65250375; 李超, E-mail: gzlichao@126.com, Tel: 0851-3762995
  • 基金资助:

    本研究由国家科技部农业科技成果转化项目(2009GB2F200334), 国家现代农业产业体系建设项目(CARS-14-贵州综合试验站), 贵州省农业科技支撑项目(黔科合NY字[2011]3015), 贵州省农业攻关项目(黔科合NY字[2009]3004), 贵州省创新能力建设项目(黔科合院所创新[2010]4002)和贵州省农科院专项资金项目(黔农科院院专项[2009]044号)资助。

Genetic Diversity of Peanut Landraces in Guizhou Province

LIN Mao1,2,LI Zheng-Qiang2,ZHENG Zhi-Hong2,LÜ Jian-Wei2,MA Tian-Jin2,LI Chao3,*,KAN Jian-Quan1,*   

  1. 1 College of Food Scienc,Southwest University,Chongqing 400716, China; 2 Institute of Crops Germplasm Resources of Guizhou, Guiyang 550006, China;
    3 Institute of Oil Crops of Guizhou Province, Guiyang 550006, China
  • Received:2011-12-16 Revised:2012-04-15 Published:2012-08-12 Published online:2012-05-11
  • Contact: 阚建全, E-mail:ganjq1965@163.com, Tel:023-65250375; 李超, E-mail: gzlichao@126.com, Tel: 0851-3762995

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被引次数

11

摘要： 为进一步了解贵州花生地方品种的遗传多样性，合理、高效利用花生资源，用50对SSR引物评价了贵州不同地理来源的68份花生地方品种，结果多态性较好的41对引物共扩增出79个等位基因，平均每个引物1.93个；多态性信息量(PIC)变幅为0.045(PM43)~0.951(PM169)；Shannon信息指数变幅为0.2518(PM79)~0.6926(PM188)，平均0.5268；Nei遗传多样性指数变幅为0.1699(PM79)~0.4995(PM188)，平均0.3556。采用类平均法对欧氏距离聚类分析表明，在遗传距离为0.94时将68个花生地方品种分成2个大类，同一地理来源、同一粒型的地方品种的亲缘关系并不是最近的，表明地方品种间亲缘关系与地理来源关系不大。说明贵州花生地方品种具有丰富遗传多样性。

关键词: 贵州地方品种, 花生, 遗传多样性

Abstract: In order to assess the genetic diversity of peanut in Guizhou province and provide a theoretical basis for breeding, 68 accessions of peanut landracescollected from Guizhou province were evaluated by 50 SSR markers. Seventy-nine alleles were amplified by forty-one polymorphic SSR primer pairs. The average number of alleles per locus was 1.93 with a range from 0.045 to 0.951. Total Shannon information index per locus varied widely from 0.2518 (PM79) to 0.6826 (PM188) with an average value of 0.5268; total Nei’s genetic diversity index per locus varied widely between 0.1699(PM79) and 0.4995 (PM188) with an average value of 0.3556. Cluster analysis with UPGMA showed that the landraces could be divided into two groups and the genetic relationship among materials with similar seed sizes was not in accord with their geographical origins. The peanut landrace in Guizhou showed higher genetic diversity.

Key words: Guizhou landrace, Peanut, Genetic diversity

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