欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (08): 1387-1396.doi: 10.3724/SP.J.1006.2012.01387

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

贵州省花生地方品种的遗传多样性

林茂1,2，李正强2，郑治洪2，吕建伟2，马天进2，李超3,*，阚健全1,*   

  1. 1西南大学食品科学学院，重庆400716；2贵州省农作物品种资源研究所，贵州贵阳550006; 3贵州省油料研究所，贵州贵阳550006
  • 收稿日期:2011-12-16 修回日期:2012-04-15 出版日期:2012-08-12 网络出版日期:2012-05-11
  • 通讯作者: 阚建全, E-mail:ganjq1965@163.com, Tel:023-65250375; 李超, E-mail: gzlichao@126.com, Tel: 0851-3762995
  • 基金资助:

    本研究由国家科技部农业科技成果转化项目(2009GB2F200334), 国家现代农业产业体系建设项目(CARS-14-贵州综合试验站), 贵州省农业科技支撑项目(黔科合NY字[2011]3015), 贵州省农业攻关项目(黔科合NY字[2009]3004), 贵州省创新能力建设项目(黔科合院所创新[2010]4002)和贵州省农科院专项资金项目(黔农科院院专项[2009]044号)资助。

Genetic Diversity of Peanut Landraces in Guizhou Province

LIN Mao1,2,LI Zheng-Qiang2,ZHENG Zhi-Hong2,LÜ Jian-Wei2,MA Tian-Jin2,LI Chao3,*,KAN Jian-Quan1,*   

  1. 1 College of Food Scienc,Southwest University,Chongqing 400716, China; 2 Institute of Crops Germplasm Resources of Guizhou, Guiyang 550006, China;
    3 Institute of Oil Crops of Guizhou Province, Guiyang 550006, China
  • Received:2011-12-16 Revised:2012-04-15 Published:2012-08-12 Published online:2012-05-11
  • Contact: 阚建全, E-mail:ganjq1965@163.com, Tel:023-65250375; 李超, E-mail: gzlichao@126.com, Tel: 0851-3762995

PDF

1800

被引次数

11

摘要： 为进一步了解贵州花生地方品种的遗传多样性，合理、高效利用花生资源，用50对SSR引物评价了贵州不同地理来源的68份花生地方品种，结果多态性较好的41对引物共扩增出79个等位基因，平均每个引物1.93个；多态性信息量(PIC)变幅为0.045(PM43)~0.951(PM169)；Shannon信息指数变幅为0.2518(PM79)~0.6926(PM188)，平均0.5268；Nei遗传多样性指数变幅为0.1699(PM79)~0.4995(PM188)，平均0.3556。采用类平均法对欧氏距离聚类分析表明，在遗传距离为0.94时将68个花生地方品种分成2个大类，同一地理来源、同一粒型的地方品种的亲缘关系并不是最近的，表明地方品种间亲缘关系与地理来源关系不大。说明贵州花生地方品种具有丰富遗传多样性。

关键词: 贵州地方品种, 花生, 遗传多样性

Abstract: In order to assess the genetic diversity of peanut in Guizhou province and provide a theoretical basis for breeding, 68 accessions of peanut landracescollected from Guizhou province were evaluated by 50 SSR markers. Seventy-nine alleles were amplified by forty-one polymorphic SSR primer pairs. The average number of alleles per locus was 1.93 with a range from 0.045 to 0.951. Total Shannon information index per locus varied widely from 0.2518 (PM79) to 0.6826 (PM188) with an average value of 0.5268; total Nei’s genetic diversity index per locus varied widely between 0.1699(PM79) and 0.4995 (PM188) with an average value of 0.3556. Cluster analysis with UPGMA showed that the landraces could be divided into two groups and the genetic relationship among materials with similar seed sizes was not in accord with their geographical origins. The peanut landrace in Guizhou showed higher genetic diversity.

Key words: Guizhou landrace, Peanut, Genetic diversity

[1]Shandong Peanut Research Institute(山东花生研究所). Peanut Cultivation in China (中国花生栽培学). Shanghai: Shanghai Scientific and Technical Publishers, 1982. pp 7–8 (in Chinese)

[2]Li Z-Q(李正强). The dissemination and application of new peanut varieties bred in Guizhou. J Peanut Sci (花生学报), 2003, 32(4): 27–30 (in Chinese with English abstract)

[3]Subramanian V, Gurtu S, Rao R C N, Nigam S N. Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay. Genome, 2000, 43: 656–660

[4]Tang R H, Gao G Q, He L Q, Han Z Q, Shan S H, Zhong R C, Zhou C Q, Jiang J, Li Y R, Zhuang W J. Genetic diversity in cultivated groundnut based on SSR markers. J Genet Genom, 2007, 34: 449–459

[5]Han Z-Q(韩柱强), Gao G-Q(高国庆), Wei P-X(韦鹏宵), Tang R-H(唐荣华), Zhong R-C(钟瑞春). Analysis of DNA polymorphism and genetic relationships in cultivated peanut (Arachis hypogaea L.) using micro-satellite markers. Acta Agron Sin (作物学报), 2004, 30(11): 1097–1101 (in Chinese with English abstract)

[6]Hong Y-B(洪彦彬), Liang X-Q(梁炫强), Chen X-P(陈小平), Lin K-Y(林坤耀), Zhou G-Y(周桂元), Li S-X(李少雄), Liu H-Y(刘海燕). Genetic differences in peanut cultivated types (Arachis hypogaea L.) revealed by SSR polymorphism. Mol Plant Breed (分子植物育种), 2008, 6(1): 71–78 (in Chinese with English abstract)

[7]Naito Y, Suzuki S, I wata Y, Kuboyama T. Genetic diversity and relationship analysis of peanut germplasm using SSR markers. Breed Sci, 2008, 58(3): 293–300

[8]Jiang H F, Liao B S, Reng X P, Lei Y, Mace E, Fu T D, Crouch J H. Comparative assessment of genetic diversity of peanut (Arachis hypogaea L.) genotypes with various levels of resistance to bacteria wilt through SSR and AFLP analyses. J Genet Genom, 2007, 34: 544–554

[9]He G H, Channapatna P. Evaluation of genetic relationships among botanical varieties of cultivated peanut (Arachis hypogaea L.) using AFLP markers. Genet Resour Crop Evol, 2001, 48: 347–352

[10]He G H, Meng R H, Gao H, Guo B Z, Gao G Q, Melanie N, Roy N P, and Prakash C. S. Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L.). Euphytica, 2005, 142: 131–136

[11]Chen B-Y(陈本银), Jiang H-F(姜慧芳), Liao B-S( 廖伯寿), Ren X-P(任小平), Huang J-Q(黄家权), Lei Y(雷永), Wang S-Y(王圣玉). Genetic diversity analysis of arachis gerplasm by SSR. J Trop Subtrop Bot (热带亚热带植物学报), 2008, 16(4): 296–303 (in Chinese with English abstract)

[12]Jiang H-F(姜慧芳), Ren X-P(任小平), Liao B-S(廖伯寿), Huang J-Q(黄家权), Lei Y(雷永), Chen B-Y(陈本银), Holbpook C C, Upachyaya H D. Peanut core collection established in china and compared with ICRISAT mini core collection. Acta Agron Sin (作物学报), 2008, 34(1): 25–30 (in Chinese with English abstract)

[13]Jiang H-F(姜慧芳), Ren X-P(任小平), Zhang X-J(张晓杰), Huang J-Q(黄家权), Wang S-Y(王圣玉), Lei Y(雷永), Yan L-Y(晏立英), Liao B-S(廖伯寿). Genetic diversity of peanut mini core collection detected by SSR markers. Chin J Oil Crop Sci (中国油料作物学报), 2010, 32( 4): 472–478 (in Chinese with English abstract)

[14]Jiang H-F(姜慧芳), Ren X-P(任小平), Zhang X-J(张晓杰), Huang J-Q(黄家权), Lei Y(雷永), Yan L-Y(晏立英), Liao B-S(廖伯寿), Upachyaya H D, Holbpook C C. Comparison of genetic diversity between peanut mini core collections from China and ICRISAT by SSR markers. Acta Agron Sin (作物学报), 2010, 36(7): 1084–1091 (in Chinese with English abstract)

[15]Jiang H-F(姜慧芳), Ren X-P(任小平), Chen Y-N(陈玉宁), Zhang X-J(张晓杰), Huang J-Q(黄家权), Liao B-S(廖伯寿). Comparison of genetic diversity between landraces and breeding varieties in Chinese peanut mini core collection by SSR markers. Acta Bot Boreali-Occident Sin (西北植物学报), 2011: 31(8): 1551–1559 (in Chinese with English abstract)

[16]Chen J(陈静), Hu X-H(胡晓辉), Miao H-R(苗华荣), Shi Y-Q(石运庆), Chi Y-C(迟玉成), Cui F-G(崔凤高), Wu L-R(吴兰荣), Yu S-L(禹山林). SSR-based analysis of genetic diversity of peanut (Arachis hypogaea L.) varieties from the region tests of Shandong province. Chin Agric Sci Bull (中国农学通报), 2008, 1(24): 105–110 (in Chinese with English abstract)

[17]Wang L-L(王丽丽), Li S-X(李尚霞), Wang Y-F(王月福). Genetic diversity of Shandong peanut popularized in different times by coefficient of parentage. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(1): 46–50 (in Chinese with English abstract)

[18]Krishna G K, Zhang J, Burow M, Pittman R N, Delikostadinov S G, Lu Y Z, Puppala N. Genetic diversity analysis in peanut (Arachis hypogaea L.) using microsatellite markers. Cell Mol Biol Lett, 2004, 9: 685–697

[19]Ferguson M E, Bramel P J, Chandra S. Gene diversity among botanical varieties in peanut (Arachis hpogaea L.). Crop Sci, 2004, 44: 1847–1854

[20]Tang R-H(唐荣华), He L-Q(贺梁琼), Zhuang W-J(庄伟建), Hang Z-Q(韩柱强), Zhong R-C(钟瑞春), Zhou C-Q(周翠球), Gao G-Q(高国庆), Li Z(李忠). Phylogenetic relationships in the genus Arachis based on SSR molecular marker profiles. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(2): 142–147 (in Chinese with English abstract)

[21]Zhang J-C(张建成), Wang C-T(王传堂), Yang X-D(杨新道). SSR and STS polymorphisms in seed testing for peanut cultivars. J Plant Genet Resour (植物遗传资源学报), 2006, 7(2): 215–219 (in Chinese with English abstract)

[22]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 1979, 76: 5269–5273

[23]Yin D-M(殷冬梅), Wang Y(王允), Shang M-Z(尚明照), Cui D-Q(崔党群). Genetic diversity analysis of peanut genotypes based on molecular markers. Sci Agric Sin (中国农业科学), 2010, 43(11): 2220–2228 (in Chinese with English abstract)

[24]Jiang H-F(姜慧芳), Ren X-P(任小平), Zhang X-J(张晓杰), Huang J-Q(黄家权), Lei Y(雷永), Yan L-Y(晏立英), Liao B-S(廖伯寿). Comparison of genetic diversity between peanut mini core collections from china and ICRISAT by SSR markers. Acta Agron Sin (作物学报), 2010, 36(7): 1084–1091 (in Chinese with English abstract)

[25]Chen J(陈静), Hu X-H(胡晓辉), Miao H-R(苗华荣), Shi Y-Q(石运庆), Yu S-L(禹山林). Analysis of genetic diversity of peanut (Arachis hypogaea L.) varieties under the region tests of north China based on SSR marker. J Plant Genet Resour (植物遗传资源学报), 2009, 10(3): 360–366 (in Chinese with English abstract)

[26]Hu X-H(胡晓辉), Wu L-R(吴兰荣), Miao H-R(苗华荣), Shi Y-Q(石运庆), Cui F-G(崔凤高), Chen J(陈静). Genetic diversity of 33 peanut varieties. J Peanut Sci (花生学报), 2010, 39 (2): 24–28 (in Chinese with English abstract)

[27]Wang C-T(王传堂), Yang X-D(杨新道), Yu X(于翔), Zhang J-C(张建成), Liu G-Z(刘光臻), Xu J-Z(徐建芝). Utilization of DNA molecular marker technology in the discrimination of peanut cultivars. Acta Agric Boreali-Sin (华北农学报), 2006, 21: 110–113 (in Chinese with English abstract)
[1] 郑玉珍, 齐飞艳, 孙子淇, 刘华, 秦利, 石磊, 王娟, 汪蒙蒙, 韩锁义, 徐静, 苗利娟, 黄冰艳, 董文召, 郑峥, 张新友. 花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J]. 作物学报, 2026, 52(6): 1646-1657.
[2] 陆一楚, 李振影, 买春海, 赵晓蕊, 王利祥. 夜间复合体关键基因AhLUX1正向调控花生结瘤的功能研究[J]. 作物学报, 2026, 52(6): 1658-1668.
[3] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[4] 谷春苗, 王润风, 黄璐, 刘浩, 鲁清, 李海芬, 李少雄, 何双呈, 洪彦彬, 陈小平, 谭斌, 余倩霞. 花生WOX基因家族的全基因组分析及不定芽再生候选基因的鉴定[J]. 作物学报, 2026, 52(5): 1326-1340.
[5] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[6] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[7] 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
[8] 于天一, 王春晓, 肖丽, 钟召迪, 王宣仓, 赵勇, 路亚, 吴月, 吴正锋. 不同结瘤特性花生品种氮素累积、产量及品质特性对氮肥用量的响应[J]. 作物学报, 2026, 52(3): 881-894.
[9] 张胜忠, 李国卫, 戈立江, 王菲菲, 胡晓辉, 苗华荣, 李燕, 钟文, 陈静. 花生机械脱壳损伤相关农艺指标筛选与QTL定位[J]. 作物学报, 2026, 52(2): 644-652.
[10] 王菲菲, 张胜忠, 杨贵华, 苗华荣, 胡晓辉, 张则林, 刘莎莎, 乔利仙, 单世华, 陈静. 331份花生种质苗期耐盐性综合评价和强耐盐种质鉴选[J]. 作物学报, 2026, 52(1): 279-294.
[11] 金欣欣, 宋亚辉, 苏俏, 杨永庆, 王瑾. 高产高油高油酸花生品种的生长发育及干物质生产特征[J]. 作物学报, 2026, 52(1): 191-201.
[12] 迟晓元, 刘庆, 张君, 赵旭红, 李美, 于天一, 潘丽娟, 许静, 姜骁, 殷祥贞, 马俊卿, 陈娜. 不同花生品种(系)耐盐碱性田间鉴定及各性状指标相关性研究[J]. 作物学报, 2026, 52(1): 85-98.
[13] 孙辰硕, 张月, 田泽锴, 晏立英, 康彦平, 陈玉宁, 王欣, 淮东欣, 王前前, 姜慧芳, 罗怀勇, 黄莉, 廖伯寿, 王志慧, 雷永. 花生种质果柄强度的遗传分化与主要影响因子分析[J]. 作物学报, 2026, 52(1): 118-130.
[14] 梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370.
[15] 王天译, 杨绣娟, 赵佳佳, 郝宇琼, 郑兴卫, 武棒棒, 李晓华, 郝水源, 郑军. 山西小麦醇溶蛋白多样性及其对面粉品质效应研究[J]. 作物学报, 2025, 51(7): 1784-1800.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2