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作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1760-1765.doi: 10.3724/SP.J.1006.2012.01760

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

国家大豆区域试验品种(系) SSR位点纯合度分析

关荣霞1，方宏亮2，何艳琴3，常汝镇1，邱丽娟1,*   

  1. 1中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程 / 农业部北京大豆生物学重点实验室，北京100081；2蚌埠医学院，安徽蚌埠 233030；3农业部全国农业技术推广服务中心，北京 100026
  • 收稿日期:2012-01-06 修回日期:2012-06-10 出版日期:2012-10-12 网络出版日期:2012-07-27
  • 通讯作者: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-82105843
  • 基金资助:

    本研究由国家自然科学基金项目(30971801)和国家重点基础研究发展计划(973计划)项目(2009CB118400)资助。

Molecular Homozygosity Analysis of Soybean Varieties in Regional Test of China by Using SSR Markers

GUAN Rong-Xia1,FANG Hong-Liang2,HE Yan-Qin3，CHANG Ru-Zhen1,QIU Li-Juan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Bengbu Medical College, Bengbu 233030, China; 3 National Agriculture Technology Extension Center, Agriculture Ministry, Beijing 100026, China
  • Received:2012-01-06 Revised:2012-06-10 Published:2012-10-12 Published online:2012-07-27
  • Contact: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-82105843

PDF

1487

被引次数

20

摘要：

利用30对SSR引物，检测2005—2009年参加国家区域试验的1 068份大豆品种(系)的位点纯合度。每年区试品种(系)的平均纯合度为94.9%~97.6%，纯合度高于85%和90%的品种(系)所占比例分别为95%和91.4%，纯合度低于85%的品种(系)有42份(占3.93%)，主要为北方春大豆和黄淮夏大豆。位点纯合度低于85%的参试品种(系)的产量比较表明，只有11份品种(系)比对照增产5%以上，20份比对照减产0.04%~13.08%；与纯合度为100%的材料比较发现，位点纯合度较低的材料在区试中产量也较低。建议国家区试品种(系)纯合度标准不低于90%，以保证审定品种的特征特性，为大豆新品种的持续推广利用提供科技支撑。

关键词: 区域试验, 大豆, SSR位点, 纯合度

Abstract:

Genomic homozygosity of seeds is very important for protection of breeder’s right and sustainable production of agriculture. Highly informative microsatellite (SSR) markers, widely used in soybean genetic mapping and genetic diversity research, are useful for soybean genomic analysis. Thirty pairs of SSR primers were used to test the DNA of 1 068 soybean varieties (lines) from regional trials of China. The average SSR locus homozygosity was 94.9–97.6% each year. Among the 1 068 varieties (lines) in China regional test screened in five years, the soybean varieties (lines) with homozygosity higher than 85% and 90% accounted for 95% and 91.4%, respectively. Only 42 (3.93%) of the varieties (lines) had homozygosity less than 85%, which were mostly from North spring and Huanghuai summer soybean planting areas. Among the 42 varieties (lines) with homozygosity less than 85% only 11 had the yield 5% more than the controls, 20 had a yield reduction of 0.04% to 13.08%. Compared with those with 100% homozygosity, varieties with lower SSR homozygosity also had lower yield in regional trials. It is suggested that criterion of soybean homozygosity in the national regional test should not be less than 90% so as to insure the protection and sustainable utilization of new soybean varieties (lines) in production.

Key words: Regional test, Soybean [Glycine max (L.) Merr.], SSR loci, Homozygosity

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