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作物学报 ›› 2013, Vol. 39 ›› Issue (01): 184-189.doi: 10.3724/SP.J.1006.2013.00184

• 研究简报 • 上一篇    

蕾期低温及湿度胁迫对Bt棉杀虫蛋白表达量的影响

陈源,顾超,王桂霞,吕春花,刘晓飞,张祥,陈德华*   

  1. 陈德华, E-mail: dehuachen2002@yahoo.com.cn, Tel: 0514-87979357
  • 收稿日期:2012-04-18 修回日期:2012-09-05 出版日期:2013-01-12 网络出版日期:2012-11-14
  • 基金资助:

    本研究由国家自然科学基金项目(30971727, 31171479), 江苏省重点实验室重大项目(10KJA210057), 教育部博导基金(20113250110001), 江苏省青蓝工程和扬州大学新世纪学术带头人基金, 江苏省三新工程项目[Sx(2011)099]和江苏高校优势学科建设工程资助项目基金资助。

Effect on Stresses of 18°C and Different Relative Humidities on Bt Protein Expression at Squaring Stage in Bt Cotton

CHEN Yuan,GU Chao,WANG Gui-Xia,LÜ Chun-Hua,LIU Xiao-Fei,ZHANG Xiang,CHEN De-Hua*   

  1. 陈德华, E-mail: dehuachen2002@yahoo.com.cn, Tel: 0514-87979357
  • Received:2012-04-18 Revised:2012-09-05 Published:2013-01-12 Published online:2012-11-14

摘要:

Bt基因来源于美国和我国的常规棉花品种DP410B和泗抗1号、杂交种岱杂1号和泗抗3号为材料,应用盆栽试验, 探讨蕾期18低温及不同湿度不同时间胁迫对叶片Bt杀虫蛋白表达量的影响。结果表明,蕾期18下不同湿度6 h胁迫对叶片Bt蛋白的含量都没有显著影响,但48 h胁迫导致2个不同Bt来源常规品种的叶片Bt杀虫蛋白表达量显著下降,与对照相比,下降6.8%~7.2%,杂交种泗抗3号也明显下降,岱杂1号则未受影响;18下高湿度与低湿度胁迫间没有显著影响。因此蕾期长时低温会对Bt棉抗虫性有一定影响,影响程度与品种及类型密切相关。

关键词: Bt棉, 低温, 湿度, Bt蛋白含量

Abstract:

The objective of this study was to investigate the effect of stresses of 18°C and different relative humidities on the leaf resistance to the insect at squaring stage in Bt cotton. The two conventional cultivars (DP410B and Sikang 1) and two hybrids (Daiza 1 and Sikang 3) from the US and China, respectively, were used. The potted cotton plants were stressed at 18°C and different relative humidities (90%, 70%, and 50%) for 6 hours and 48 hours, respectively, then we determined leaf Bt protein contents. The results showed that the short period (6 hours) stresses of 18°C and different relative humidities obviously had no effect on the leaf Bt protein contents. but the leaf Bt protein contents sharply reduced in the two Bt conventional cultivars after the longer period (48 hours) stresses of 18°C and different relative humidities compared with the control, with the reduction from 6.8% to 7.2%. The leaf Bt protein content of hybrid Sikang 3 markedly decreased while that of hybrid Daiza 1 remained unchanged. There was no difference in the effect between the high relative humidity and low relative humidity at 18°C, so the low temperature duration may affect the Bt cotton resistance to insect at squaring stage, which is closely related to cultivars.

Key words: Bt cotton, Low temperature, Relative humidity, Bt protein content

[1]Guo X-M(郭香墨), Fan S-L(范术丽), Wang H-M(王红梅), Yang G-T(严根土). Achievements of technical innovation about cotton genetics and breeding in China. Cotton Sci (棉花学报), 2007, 19(5): 323–330 (in Chinese with English abstract)



[2]Li D-J(李德军). The development of transgenic Bt cotton, the survey of insect pest occurrence and the opportunity of pesticide industry. China Agrochems (中国农药), 2007, 6: 29–36 (in Chinese)



[3]Zhang J(张俊), Guo X-M(郭香墨), Ma L-H(马丽华). Study on effects of different foreign Bt and Bt+CpTI insect-resistant genes on bollworm resistance in upland cotton (G. Hirsutum L.). Cotton Sci (棉花学报), 2002, 14(3): 158–161 (in Chinese with English abstract)



[4]Finnegan E J, Liewellyn D J, Fitt G P. What’s Happening to the expression of the insect protection in field-grown ingard cotton? In: the 11th Australian Cotton Conference, 2002. pp 291–297



[5]Chen S(陈松), Wu J-Y(吴敬音), Zhou B-L(周宝良), Huan J-Q(黄骏麒), Zhang R-X(张荣铣). On the temporal and spatial expression of Bt toxin protein in Bt transgenic cotton. Acta Gossypii Sin (棉花学报), 2000, 12(4): 189–193 (in Chinese with English abstract)



[6]Xing C-Z(邢朝柱), Jing S-R(靖深蓉), Cui X-F(崔学芬), Guo L-P(郭立平), Wang H-L(王海林), Yuan Y-L(袁有禄). The spation-temporal distribution of Bt insecticidal protein and the effect of transgenic Bt cotton on bollworm resistance. Acta Gossypii Sin (棉花学报), 2001, 13(1): 11–15(in Chinese with English abstract)



[7]Chen D H, Ye G Y, Yang C Q, Chen Y, Wu Y K. Effect of introducing Bacillus thuringiensis gene on nitrogen metabolism in cotton. Field Crops Res, 2005: 1: 1–9



[8]Benedict J H, Sachs E S, Altman D W, Deaton W R, Kohel R J, Ring D R, Berberich S A. Field performance of cottons expressing transgenic CryIA insecticidal proteins for resistance to Helicoverpazca. J Econ Entomol, 1996, 89: 230–238



[9]Pettigrew W T, Adamczyk J J. Nitrogen fertility and planting date effects on lint yield and Cry1Ac (Bt) endotoxin production. Agron J, 98: 691–697



[10]Zhou D-S(周冬生), Wu Z-T(吴振廷), Wang X-L(王学林), Zheng H-J(郑厚今), Xia J(夏静). Influence of soil stress and temperature on resistance to cotton bollworm of transgenic Bt cotton. Cotton Sci (棉花学报), 2001, 13(4): 290–292 (in Chinese with English abstract)



[11]Zhou D-S(周冬生), Wu Z-T(吴振廷), Wang X-L(王学林), Ni C-G(倪春耕), Zheng H-J(郑厚今), Xia J(夏静). Effect of temperature and nitrogen fertilizer on insect resistance of Bt cotton. J Anhui Agric Univ (安徽农业大学学报), 2000, 27(4): 352–357 (in Chinese with English abstract)



[12]Luo Z, Dong H Z, Li W J, Zhao M, Zhu Y Q. Individual and combined effects of salinity and waterlogging on CrylAc expression and insecticidal efficacy of Bt cotton. Crop Protect, 2008, 27: 1485–1490



[13]Wang L-M(王留明), Wang J-B(王家宝), Sheng F-F(沈法富), Zhang X-S(张学坤), Liu R-Z(刘任重). Influences of waterlogging and drought on different transgenic Bt cotton cultivars. Acta Gossypii Sin (棉花学报), 2001, 13(2): 87–90 (in Chinese with English abstract)



[14]Wang B-M(王保民), He Z-P(何钟佩), Tian X-L(田晓莉). Preparation of monoclonal antibodies of CryIA insecticidal crystal protein and its application in determining toxin level in Bt cotton. Acta Gossypii Sin (棉花学报), 2000, 12(1): 34–39 (in Chinese with English abstract)



[15]Gasser C S, Fraley R T. Genetically engineering plants for crop improvement. Science, 1989, 244: 1293–1299



[16]Olsen K M, Daly J C. Plant-toxin interactions in transgenic Bt cotton and their effect on mortality of Helicoverpa armigera (Lepidoptera: Noctuidae). J Econ Entomol, 2000, 4: 1293–1299



[17]Stam M, Mol J N M, Kooter J M. The silence of genes in transgenic plants. Annal Bot, 1997, 79: 3–12



[18]Adamczyk J J, Meredith W R. Genetic basis for the variability of Cry1Ac expression among commercial transgenic Bacillus thuringiensis (Bt) cotton cultivars in the United States. J Cotton Sci, 2004, 8: 17–23



[19]Hallikeri S S, Halemani H L, Katageri I S, Patil B C, Patil V C, Palled Y B. Influence of sowing time and moisture regimes on cry protein concentration and related parameters of Bt-cotton. Karnataka J Agric Sci, 2009, 22: 995–1000



[20]Ian J R. Effect of genotype, edaphic, environmental conditions, and agronomic practices on CryIAc protein expression in transgenic cotton. J Cotton Sci, 2006, 10: 252–262



[21]Adamczyk J J, Hardee D D, Adams L C, Sumerford D V. Correlating differences in larval survival and development of bollworms (Lepidoptera: Noctuidae) and fall armyworms (Lepidoptera: Noctuidae) to differential expression of Cry1Ac delta-endotoxin in various plant parts among commercial cultivars of transgenic Bacillus thuringiensis cotton. J Econ Entomol, 2001, 94: 284–290



[22]Adamczyk J J, Meredith W R. Genetic basis for the variability of Cry1Ac expression among commercial transgenic Bacillus thuringiensis (Bt) cotton cultivars in the United States. J Cotton Sci, 2004, 8: 17–23



[23]Chen D H, Ye G Y, Yang C Q, Chen Y, Wu Y K. The effect of high temperature on the insecticidal properties of Bt cotton. Environ Exp Bot, 2005, 53: 333–342



[24]Zhang X, Ye G Y, Zhang L, WangY H, Chen Y, Chen D H. The impact of introducing the Bacillus thuringiensis gene into cotton on boll nitrogen metabolism. Environ Exp Bot, 2007, 61: 175–180

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