作物学报 ›› 2013, Vol. 39 ›› Issue (04): 626-631.doi: 10.3724/SP.J.1006.2013.00626
桑贤春,杜川,王晓雯,杨正林,凌英华,赵芳明,李云峰,何光华*
SANG Xian-Chun,DU Chuan,WANG Xiao-Wen,YANG Zheng-Lin,LING Ying-Hua,ZHAO Fang-Ming,LI Yun-Feng,HE Guang-Hua*
摘要:
利用EMS诱变籼型水稻恢复系缙恢10号,获得一个稳定遗传的矮化脆性突变体dbc1,苗期即表现矮化、叶片变脆,一直保持到成熟。与原始亲本相比,突变体的各节间均显著缩短,株高仅58.93 cm,略有包穗,属于dn型矮化变异,对赤霉素的敏感性显著下降,有效穗、千粒重和结实率无明显变化,穗长、穗粒数和实粒数则极显著下降。进一步分析发现,dbc1的茎秆和叶片的载荷强度极显著下降,纤维素含量无变化,木质素含量则略有下降,差异达显著水平。遗传分析表明该性状受1对隐性核基因调控,利用886株西农1A/dbc1的F2变异单株,最终把DBC1基因定位在第2染色体SSR标记RM13943和RM13952之间,物理距离仅197 kb,含有52个注释基因。这为下一步调控基因的克隆和dbc1材料的育种应用奠定了基础。
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