作物学报 ›› 2013, Vol. 39 ›› Issue (04): 753-759.doi: 10.3724/SP.J.1006.2013.00753

• 研究简报 • 上一篇    



  1. 沈阳农业大学水稻研究所 / 农业部东北水稻生物学与遗传育种重点实验室 / 教育部与辽宁省共建北方粳稻遗传育种重点实验室,辽宁沈阳110866
  • 收稿日期:2012-09-10 修回日期:2012-12-11 出版日期:2013-04-12 网络出版日期:2013-01-28
  • 通讯作者: 陈温福, E-mail: wfchen5512@yahoo.com.cn
  • 基金资助:


Responses of Antioxidant System to Long-Term Cold Water Stress in New Rice Line J07-23 with Strong Cold Tolerance

WANG Guo-Jiao**,WANG Jia-Yu**,MIAO Wei,ZHAO Ming-Hui,CHEN Wen-Fu*   

  1. Rice Research Institute of Shenyang Agricultural University / Key Laboratory of Northeast Rice Biology, Genetics and Breeding, Ministry of Agriculture / Key Laboratory of Northern Japonica Rice Breeding, Ministry of Education, Shenyang 110866, China
  • Received:2012-09-10 Revised:2012-12-11 Published:2013-04-12 Published online:2013-01-28
  • Contact: 陈温福, E-mail: wfchen5512@yahoo.com.cn


为了阐明强耐冷性水稻对长期冷水胁迫抗性的生理基础, 以水稻新品系J07-23和冷敏感的栽培稻秀子糯为试验材料, 研究长期冷水胁迫(19℃)下参试材料在开花期剑叶活性氧代谢、抗氧化酶活性、抗氧化剂含量的变化以及成熟后的穗部性状。结果表明, 在长期冷水胁迫下, 秀子糯剑叶的O2产生速率、H2O2含量和MDA含量显著增加, 抗氧化系统中SOD、CAT和GR活性显著升高; J07-23剑叶H2O2含量显著增加, 而O2产生速率和MDA含量无显著变化。J07-23的抗氧化酶(SOD、CAT、POD、APX和GR)活性极显著升高, 抗氧化剂(AsA和GSH)含量及AsA/DHA值和GSH/GSSG值也显著增加。依据耐冷性评价标准, J07-23具有极强耐冷性, 冷水胁迫下高的抗氧化酶活性和抗氧化剂含量是其耐冷性的生理生化基础。

关键词: 水稻, 冷水胁迫, 活性氧, 抗氧化系统


 In order to clarify physiological basis of cold tolerance for rice (Oryza sativa L.) with strong cold tolerance under long-term cold water stress, new rice line J07-23 and chilling-sensitive cultivated rice Xiuzinuo were used to understand effects of long-term cold water stress (19℃) on reactive oxygen species metabolism, antioxidant enzyme activities, changes of antioxidants contents of flag leaves at flowering stage and panicle traits at maturity in this study. The results showed that O2?generating rate, H2O2 content and MDA content of flag leaves increased significantly in Xiuzinuo under long-term cold water stress and its SOD, CAT and GR activities also increased significantly. H2O2 content of flag leaves increased significantly in J07-23, but there were no significant changes in O2? generating rate and MDA content. Antioxidant enzyme (SOD, CAT, POD, APX, and GR) activities increased very significantly, and antioxidants (AsA and GSH) contents, and AsA/DHA ratio and GSH/GSSG ratio also increased significantly in J07-23. J07-23 has extremely strong tolerance to cold stress according to the evaluation standard of cold tolerance. High antioxidant enzyme activities and antioxidants contents are the physiological and biochemical basis of cold tolerance for J07-23 under cold water stress.

Key words: Rice (Oryza sativa L.), Cold water stress, Reactive oxygen species, Antioxidant system

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