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作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1319-1324.doi: 10.3724/SP.J.1006.2013.01319

• 研究简报 • 上一篇    下一篇

外源甜菜碱对干旱胁迫下小麦幼苗叶绿体抗氧化酶及psbA基因表达的调节

侯鹏飞1,马俊青2,赵鹏飞1,张欢玲1,赵会杰1,刘华山1,赵一丹1,汪月霞1,*   

  1. 1河南农业大学生命科学学院, 河南郑州 450002;  2河南省林业科学研究院, 河南郑州 450008
  • 收稿日期:2012-12-03 修回日期:2013-03-11 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 汪月霞, E-mail: yxwang2100@126.com
  • 基金资助:

    本研究由国家自然科学基金项目(31000688)和河南省教育厅科学技术研究重点项目(13A210487)资助。

Effects of Betaine on Chloroplast Protective Enzymes and psbA Gene Expression in Wheat Seedlings under Drought Stress

HOU Peng-Fei1,MA Jun-Qing2,ZHAO Peng-Fei1,ZHANG Huan-Ling1,ZHAO Hui-Jie1,LIU Hua-Shan1,ZHAO Yi-Dan1,WANG Yue-Xia1,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 Henan Academy of Forestry, Zhengzhou 450008, China
  • Received:2012-12-03 Revised:2013-03-11 Published:2013-07-12 Published online:2013-04-23
  • Contact: 汪月霞, E-mail: yxwang2100@126.com

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9

摘要：

为了探究外源甜菜碱对干旱胁迫下小麦幼苗的生长调节作用，以优质高产、高抗旱的小麦品种矮抗58为材料，四叶期分别用0.1、1.0和10.0 mmol L−1的GB预处理小麦叶片，同时在根部施加30%聚乙二醇(PEG-6000)以模拟干旱环境，研究其对小麦超氧化物歧化酶(SOD)，过氧化氢酶(CAT)，过氧化物酶(POD)，丙二醛(MDA)、超氧阴离子自由基()产生速率、叶绿素含量及相对含水量的影响，并采用Real-time PCR测定叶绿体psbA基因表达水平的变化。结果表明，干旱胁迫明显减少小麦叶片相对含水量和叶绿素含量，降低SOD、CAT及POD活性，提升MDA含量和O2?产生速率，抑制psbA基因表达水平，而外施GB具一定浓度效应，在适当浓度下能明显缓解这些胁迫反应，调控干旱胁迫下小麦叶绿体抗氧化酶活性以清除多余活性氧，减缓相对含水量及叶绿素含量的降低，提升psbA基因的表达水平，从而加快受损D1蛋白的周转并提高小麦的抗干旱胁迫能力。

关键词: 小麦, 甜菜碱, psbA基因, 干旱胁迫, 叶绿体抗氧化酶

Abstract:

With the purpose of revealing the effect of exogenous glycine betaine (GB) on wheat seedlings, Aikang 58 was used as the experimental material, which has the characteristics of high yield and drought resistance. The leaves were sprayed with 0.1, 1.0, and 10.0 mmol L−1 of GB during four-leaf stage, respectively, while the root was applied by 30% polyethylene glycol (PEG-6000) to simulate the drought environment. The effects of exogenous GB treatment on the parameters of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) activities, superoxide anion radicals () production rate, malondialdehyde (MDA), chlorophyll content, and relative water content (RWC)under drought stress were tested three days after treatment. Simultaneously, the relative transcriptional expression of psbA inchloroplast was determined by real time PCR assay. The results showed that drought stress obviously decreased the RWC and chlorophyll content of wheat leaves, reduced activities of SOD, CAT, and POD, increased MDA content and the production rate of reactive oxygen species, and suppressed psbA expression. Nevertheless, such stress reactions were alleviated by exogenous GB treatment, and this regulation effect showed a great correlation with GB concentration. These results indicated thatexogenous GB could remove excess reactive oxygen, and retard the decrease of RWC and chlorophyll content by regulating antioxidant enzyme activities in wheat chloroplast under drought stress, enhance the transcriptional level of psbA gene, accelerate the turnover of D1 protein in wheat chloroplast, and finally improve the drought resistance in wheat.

Key words: Triticum aestivum, Betaine, psbA gene, Drought stress, Chloroplast antioxidant enzyme

[1]Pandey D M, Yeo U D. Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf. Biol Plant, 2008, 52: 291–298



[2]Liu W J, Yuan S, Zhang N H, Lei T, Duan H G, Liang H G, Lin H H. Effect of water stress on photosystem II in two wheat cultivars. Biol Plant, 2006, 50: 597–602



[3]Yuan L(袁琳), Karim Ali(克热木?伊力), Zhang L-Q(张利权). Effects of NaCl stress on active oxygen metabolism and membrane stabiliyty in Pistacia vera seedlings. Acta Phytoecol Sin (植物生态学报), 2005, 29(6): 985–991 (in Chinese with English abstract)



[4]Yamamoto Y, Aminaka R, Yoshioka M, Khatoon M, Komayama K, Takenaka D, Yamashita A, Nijo N, Inagawa K, Morita N, Sasaki T. Quality control of photosystem II: impact of light and heat stresses. Photosynth Res, 2008, 98: 589–608



[5]Zhao P-F(赵鹏飞), Wang L-H(王林华), Zhao H-J(赵会杰), Liang S-R(梁书荣), Lü S-M(吕淑敏), Qu X-F(曲小菲), Wang Y-X(汪月霞). Regulation of exogenous salicylic acid on expression of chloroplast gene psbA in wheat (Triticum aestivum) leaves under heat and high irradiance stress. Plant Physiol Commun (植物生理学通讯), 2010, 46(6): 537–540 (in Chinese with English abstract)



[6]Wang Y-X(汪月霞), Suo B(索标), Zhao P-F(赵鹏飞), Qu X-F(曲小菲), Yuan L-G(袁利刚), Zhao X-J(赵雪娟), Zhao H-J(赵会杰). Effect of abscisic acid treatment on psbA gene expression in two wheat cultivars during grain filling stage under drought stress. Acta Agron Sin (作物学报), 2011, 37(8): 1372–1377 (in Chinese with English abstract)



[7]Bourot S, Sire O, Trautwetter A . Glycine betaine assisted protein folding in a lysA mutant of Escherichia coli. J Biol Chem, 2000, 275: 1050–1056



[8]Prasad KVSK, Pardha-Saradhi P. Enhance tolerance to photoinhibition in transgenic plants through targeting of glycinebetaine biosynthesis into chloroplasts. Plant Sci, 2004, 166: 1197–212



[9]Allakhverdiev S I, Los D A, Mohanty P, Nishiyama Y, Murata N. Glycinebetaine alleviates the inhibitory effect of moderate heat stress on the repair of photosystem II during photoinhibition. Biochim Biophys Acta, 2007, 1767: 1363–1371



[10]Wang G P, Hui Z, Li F, Zhao M R, Zhang J, Wang W. Improvement of heat and drought photosynthetic tolerance in wheat by overaccumulation of glycinebetaine. Plant Biotechnol Rep, 2010, 4: 213–222



[11]Schonfeld M, Johnson R, Carver B, Mornhinweg D. Water relations in winter wheat as drought resistant indicators. Crop Sci, 1988, 28: 526–531



[12]Lichtenthaler H K. Chlorophylls and carotenoids: pigments of photosynthetic biomemranes. Methods Enzymol, 1987, 148: 350–382



[13]Xu P L, Guo Y K, Bai J G, Shang L, Wang X J. Effects of long-term chilling on ultrastructure and antioxidant activity in leaves of two cucumber cultivars under low light. Physiol Plant, 2008, 132: 467–478



[14]Zhang J H, Huang W D, Liu Y P, Pan Q H. Effects of temperature acclimation pretreatment on the ultrastructure of mesophyll cells in young grape plants (Vitis vinifera L. cv. Jingxiu) under cross-temperature stresses. J Int Plant Biol, 2005, 47: 959–970



[15]Wang A-G(王爱国), Luo G-H(罗广华). Quantitative relation between the reaction of hydroxylamine and superoxide amion radicals in plants. Plant Physiol Commun (植物生理学通讯), 1990, 26(6): 55–57 (in Chinese with English abstract)



[16]Kantar M, Lucas S, Budak H. miRNA expression patterns of Triticum aestivum in response to shock drought stress. Planta, 2010, 233: 471–484



[17]Kenneth J L, Thomas D S. Analysis of relative gene expression date using real-time quantitative PCR and the 2?ΔΔCT method. METHOD Sci, 2001, 25: 402–408



[18]Patykowski J, Urbanek H. Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea. J Phytopathol, 2003, 151: 153–161



[19]Cordoba-Pedregosa M C, Cordoba F, Villalba J M, Gonzales-Reyes J A. Zonal changes in ascorbate and hydrogen peroxide contents, peroxidase, and ascorbate-related enzyme activities in onion roots. Plant Physiol, 2003, 131: 696–706



[20]Zhao L-Y(赵丽英), Deng X-P(邓西平), Shan L(山仑). The response mechanism of active oxygen species removing system to drought stress. Acta Bot Boreali-Occident Sin (西北植物学报), 2005, 25(2): 413–418 (in Chinese with English abstract)



[21]Diego M C, Oliva M A, Martinez C A, Cambraiaz C A, Cambraia J. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot , 2003, 49: 69–76



[22]Edelman M, Mattoo A K. D1-protein dynamics in photosystem II: the lingering enigma. Photosynth Res, 2008, 98: 609–620



[23]Mulo P, Sakurai I, Aro E M. Strategies for psbA gene expression in cyanobacteria, green algae and higher plants: from transcription to PSII repair. Biochim Biophys Acta, 2012, 1817: 247–257



[24]Komayama K, Khatoon M, Takenaka D, Horie J, Yamashita A, Yoshioka M, Nakayama Y, Yoshida M, Ohira S, Morita N, Velitchkova M, Enami I, Yamamoto Y. Quality control of photosystem II: cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids. Biochim Biophys Acta, 2007, 1767: 838–846



[25]Qion Q M, Wei W, Yong H L, De Q L, Qi Z. Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliar-applied glycinebetaine. J Plant Physiol, 2006, 163: 165–167
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