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作物学报 ›› 2014, Vol. 40 ›› Issue (01): 174-180.doi: 10.3724/SP.J.1006.2014. 00174

• 研究简报 • 上一篇    下一篇

利用SSR标记分析野生小豆及其近缘野生植物的遗传多样性

刘长友1,范保杰1,曹志敏1,苏秋竹1,王彦1,张志肖1,程须珍2,田静1,*   

  1. 1 河北农林科学院粮油作物研究所 / 河北省作物遗传育种实验室, 河北石家庄 050031; 2 中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2013-07-01 修回日期:2013-09-16 出版日期:2014-01-12 网络出版日期:2013-10-22
  • 通讯作者: 田静, E-mail: nkytianjing@163.com, Tel: 0311-87670655
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-09),河北省自然科学基金(C2012301012)和河北省农林科学院青年基金(A2012060102)项目资助。

Genetic Diversity Analysis of Wild Adzuki Bean Germplasm and Its Relatives by Using SSR Markers

LIU Chang-You1,FAN Bao-Jie1,CAO Zhi-Min1,SU Qiu-Zhu1,WANG Yan1,ZHANG Zhi-Xiao1,CHENG Xu-Zhen2,TIAN Jing1,*   

  1. 1 Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences / Hebei Laboratory of Crop Genetic and Breeding, Shijiazhuang 050031, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-07-01 Revised:2013-09-16 Published:2014-01-12 Published online:2013-10-22
  • Contact: 田静, E-mail: nkytianjing@163.com, Tel: 0311-87670655

摘要:

利用28SSR引物对96份野生小豆资源、小豆近缘野生植物及栽培小豆进行遗传多样性分析,共检测到255个等位变异,平均每对SSR引物9.10个,多态信息含量的变异范围从0.3740.865,平均为0.722。野生小豆材料和近缘植物Vigna minima遗传变异丰富。来自不同地域的野生小豆材料具有大量特异等位变异,基于非加权成组配对算术平均法的聚类分析可将不同地理来源的野生小豆单独分群,且与主坐标分析的结果相一致。4份栽培小豆材料与日本野生小豆遗传距离较近,表明目前国内小豆育种中较多使用了含有日本血缘的小豆材料,以及国内野生小豆资源的搜集和利用工作落后于日本。本研究对国内野生小豆资源的搜集和保存具有指导意义,并可以为这些资源的评价、利用和优异基因的发掘提供参考。

关键词: 野生小豆, SSR, 小豆近缘植物, 遗传多样性

Abstract:

Wild Adzuki bean (V. angularis var. nipponensis) germplasm and its relatives are very important materials for adzuki bean breeding. In this paper, genetic diversity of 96 accessions of wild types, relatives and cultivated adzuki beans were analyzed by using 28 pairs of polymorphic SSR makers. The results showed that a total of 255 alleles were detected in all the accessions. The average number of allele per SSR locus was 9.10. The range of genetic diversity index (polymorphic information content, PIC value) was from 0.374 to 0.865, with an average of 0.722. The results revealed that there were abundant genetic variations between wild adzuki bean and its relatives Vigna minima. Both cluster and principal coordinate analysis (PCO analysis) suggested that there was a high genetic differentiation between wild adzuki beans and its relatives, and they could be distinguished from each other clearly according to their origins, suggesting that the locations of wild adzuki bean accessions affect their genetic variations. When analyzed the relationship between adzuki bean varieties and wild adzuki bean accessions, we found that their genetic distance was more closer to Japanese wild adzuki bean accessions than to domestic accessions, indicating that more Japanese adzuki bean accessions were used in Chinese adzuki bean breeding, and showing that our works for domestic wild adzuki bean resources collection and use is behind Japan. The present study provides some information in collection and conservation of domestic wild adzuki bean accessions, and the result is useful for evaluating and using these resources to find useful genes.

Key words: Wild adzuki bean, SSR, Relatives of adzuki bean, Genetic diversity

[1]郑卓杰. 中国食用豆类学. 北京: 中国农业出版社, 1997. pp 3–6



Zheng Z J. Food Legumes in China. Beijing: Chinese Agricultural Press, 1997. pp 3–6 (in Chinese)



[2]王述民, 曹永生, R J Redden, 胡家蓬. 我国小豆种质资源形态多样性鉴定与分类研究. 作物学报, 2002, 28: 727–733



Wang S M, Cao Y S, Redden R J, Hu J P, Usher T. The morphological diversity and classification of adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] germplasm resources in China. Acta Agron Sin, 2002, 28: 727–733 (in Chinese with English abstract)



[3]田静, 范保杰. 河北省小豆品种资源主要农艺性状的遗传变异分析. 河北农业大学学报, 2002, 25(4): 17–20



Tian J, Fan B J. Genetic variability of agronomic traits of adzuki bean in Hebei province. J agric univ Hebei, 2002, 25(4): 17–20 (in Chinese with English abstract)



[4]刘长友, 田静, 范保杰. 河北省小豆种质资源遗传多样性分析. 植物遗传资源学报, 2009, 10: 73–76



Liu C Y, Tian J, Fan B J. Genetic diversity analysis of adzuki bean germplasm in Hebei Province. J Plant Genet Resour, 2009, 10: 73–76 (in Chinese with English abstract)



[5]范树国, 张再君, 刘林, 刘鸿先, 梁承邺. 中国野生稻遗传资源的保护及其在育种中的利用, 生物多样性, 2000, 8: 198–207



Fan S G, Zhang Z J, Liu L, Liu H X, Liang C Y. Conservation of wild rice genetic resources in China and their utilization in breeding. Biodiversity, 2000, 8: 198–207 (in Chinese with English abstract)



[6]章琦, 赵炳宇, 赵开军, 王春连, 杨文才, 林世成, 阙更生, 周永力, 李道远, 陈成斌, 朱立煌. 普通野生稻的抗水稻白叶枯病(Xanthomonas oryzae pv. oryzae)新基因Xa-23(t)的鉴定和分子标记定位. 作物学报, 2000, 26: 536–542



Zhang Q, Zhao B Y, Zhao K J, Wang C L, Yang W C, Lin S C, Que G S, Zhou Y L, Li D Y, Chen C B, Zhu L H. Identifying and mapping a new gene Xa-23(t) for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Acta Agron Sin, 2000, 26: 536–542 (in Chinese with English abstract)



[7]王希, 李勇, 朱延明, 柏锡, 才华, 纪巍. 野生大豆胁迫应答膜联蛋白基因的克隆及胁迫耐性分析. 作物学报, 2010, 36: 1666–1673



Wang X, Li Y, Zhu Y M, Bai X, Cai H, Ji W. Cloning and tolerance analysis of gsANN gene related to response on stress in Glycine soja. Acta Agron Sin, 2010, 36: 1666–1673 (in Chinese with English abstract)



[8]Somta P, Kaga A, Tomooka N, Isemura T, Vaughan D A, Srinives P. Mapping of quantitative trait loci for a new source of resistance to bruchids in the wild species Vigna nepalensis Tateishi & Maxted (Vigna subgenus Ceratotropis). Theor Appl Genet, 2008, 117: 621–628



[9]Siriwardhane D, Egawa Y, Tomooka N. Cross compatibility of cultivated adzuki bean (Vigna angularis) and rice bean (V. umbellata) with their wild relatives. Plant Breed, 1991, 107: 320–325



[10]金文林, 文自翔, 濮绍京, 赵波. 应用RAPD分析小豆种质资源遗传多样性及遗传演化趋势. 中国农业科学, 2005, 38: 241–249



Jin W L, Wen Z X, Pu S J, Zhao B. Genetic diversity and evolution of adzuki bean germplasm resources based on RAPD markers. Sci Agric Sin, 2005, 38: 241–249 (in Chinese with English abstract)



[11]陶宛鑫, 濮绍京, 金文林, 赵波, 万平. 野生小豆种质资源植株形态性状多样性分析. 植物遗传资源学报, 2007, 8: 174–178



TaoW X, Pu S J, Jin W L, Zhao B, Wan P. The morphological diversity analysis of wild adzuki bean germplasm resources. J Plant Genet Resour, 2007, 8: 174–178 (in Chinese with English abstract)



[12]赵波, 叶剑, 金文林, 曾潮武, 吴宝美, 濮绍京, 潘金豹, 万平. 不同类型小豆种质SSR标记遗传多样性及性状关联分析.中国农业科学, 2011, 44: 673–682



Zhao B, Ye J,Jin W L, Zeng C W, Wu B M, Pu S J, Pan J B, Wan P. Analysis on genetic diversity and trait association of different types of adzuki bean (Vigna angularis) by SSR markers. Sci Agric Sin, 2011, 44: 673–682 (in Chinese with English abstract)



[13]宗绪晓, Duancan Vaughan, Norihiko Tomooka, Akito Kaga, 王新望, 关建平, 王述民. AFLP初析小豆栽培和野生变种(Vigna angularis var.angularis and var.nipponensis)间演化与地理分布关系. 中国农业科学, 2003, 36: 367–374



Zong X X,Vaughan D, Tomooka N, Kaga A, Wang X W, Guan J P, Wang S M. Preliminary study on geographical distribution and evolutionary relationships between cultivated and wild adzuki bean (Vigna angularis var.angularis and var. nipponensis) by AFLP analysis. Sci Agric Sin, 2003, 36: 367–374 (in Chinese with English abstract)



[14]Han O K, Kaga A, Isemura T, Wang X W, Tomooka N, Vaughan D A. A genetic linkage map for adzuki bean [Vigna angularis (Willd.) Ohwi and Ohashi]. Theor Appl Genet, 2005, 111: 1278–1287



[15]Nei M, Tajima F, Tateno Y. Accuracy of estimated phylogenetic trees from molecular data: II. Gene frequency data. J Mol Evol, 1983, 19: 153–170



[16]文自翔, 金文林, 濮绍京, 赵波. 遗传标记在小豆遗传育种研究中的应用. 北京农学院学报, 2003, 18(1): 62–68



Wen Z X, Jin W L, Pu S J, Zhao B. The application of genetic marker in genetics and breeding of adzuki beans. J Beijing Agric Coll, 2003, 18(1): 62–68 (in Chinese with English abstract)



[17]王述民, 张赤红. 利用AFLP标记鉴定小豆种质遗传多样性, 植物遗传资源学报, 2002, 3(3): 1–5



Wang S M, Zhang C H. Genetic diversity of adzuki bean germplasm revealed by AFLP marker. J Plant Genet Resour, 2002, 3(3): 1–5 (in Chinese with English abstract)



[18]宗绪晓, D Vaughan, A Kaga, N Tomooka, 王新望, 关建平, 王述民. AFLP分析小豆种(Vigna angularis)内遗传多样性. 作物学报, 2003, 29: 562–568



Zong X X, Vaughan D, Kaga A, Tomooka N, Wang X W, Guan J P, Wang S M. Genetic diversity in Vigna angularis revealed by AFLP analysis. Acta Agron Sin, 2003, 29: 562–568 (in Chinese with English abstract)



[19]粟生群, 荣廷昭, 余跃辉, 刘坚. 利用AFLP标记鉴定小豆栽培型种质遗传多样性. 四川农业大学学报, 2005, 23(2): 156–162



Su S Q, Rong T Z, Yu Y H, Liu J. Genetic diversity in cultivated adzuki bean germplasm resources revealed by AFLP markers. J Sichuan Agric Univ, 2005, 23(2): 156–162 (in Chinese with English abstract)



[20]Wang X W, Kaga A, Tomooka N, Vaughan D A. The development of SSR markers by a new method in plants and their application to gene flow studies in adzuki bean [Vigna angularis (Willd.) Ohwi and Ohashi]. Theor Appl Genet, 2004, 109: 352–360



[21]徐宁, 程须珍, 王丽侠, 王素华, 刘长友, 孙蕾, 梅丽. 用于中国小豆种质资源遗传多样性分析SSR分子标记筛选及应用.作物学报, 2009, 35: 219−227



Xu N, Cheng X Z, Wang L X, Wang S H, Liu C Y, Sun L, Mei L. Screening and application of SSR molecular markers for genetic diversity analysis of Chinese adzuki bean germplasm resources. Acta Agron Sin, 2009, 35: 219−227 (in Chinese with English abstract)



[22]王丽侠, 程须珍, 王素华, 梁辉, 赵丹, 徐宁. 应用SSR标记对小豆种质资源的遗传多样性分析. 作物学报, 2009, 35: 1858−1865



Wang L X, Cheng X Z, Wang S H, Liang H, Zhao D, Xu N. Genetic diversity of adzuki bean germplasm resources revealed by SSR markers. Acta Agron Sin, 2009, 35: 1858−1865 (in Chinese with English abstract)



[23]王丽侠, 程须珍, 王素华, 徐宁, 梁辉, 赵丹. 利用SSR标记分析小豆种质资源的遗传多样性. 中国农业科学, 2009, 42: 2661–2666



Wang L X, Cheng X Z, Wang S H, Xu N, Liang H, Zhao D. Genetic diversity among adzuki bean germplasm revealed by SSR markers. Sci Agric Sin, 2009, 42: 2661–2666 (in Chinese with English abstract)



[24]王丽侠, 程须珍, 王素华. 基于SSR标记分析小豆及其近缘植物的遗传关系. 生物多样性, 2011, 19: 17–23



Wang L X, Cheng X Z, Wang S H. Genetic diversity in adzuki bean and its relatives based on SSR markers. Biodivers Sci, 2011, 19: 17–23 (in Chinese with English abstract)



[25]Xu H X, Ting J, Kaga A, Tomooka N, Isemura T, Vaughan D A. Genetic diversity of cultivated and wild azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] as assessed by SSR markers. Genome, 2008, 51: 728–738



[26]Tomooka N, Kashiwaba K, Vaughan D A, Ishimoto M, Egawa Y. The effectiveness of evaluating wild species: searching for sources of resistance to bruchid beetles in the genus Vigna subgenus Ceratotropis. Euphytica, 2000, 115: 27–41

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