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作物学报 ›› 2014, Vol. 40 ›› Issue (01): 174-180.doi: 10.3724/SP.J.1006.2014. 00174

• 研究简报 • 上一篇    下一篇

利用SSR标记分析野生小豆及其近缘野生植物的遗传多样性

刘长友1,范保杰1,曹志敏1,苏秋竹1,王彦1,张志肖1,程须珍2,田静1,*   

  1. 1 河北农林科学院粮油作物研究所 / 河北省作物遗传育种实验室, 河北石家庄 050031; 2 中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2013-07-01 修回日期:2013-09-16 出版日期:2014-01-12 网络出版日期:2013-10-22
  • 通讯作者: 田静, E-mail: nkytianjing@163.com, Tel: 0311-87670655
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-09)，河北省自然科学基金(C2012301012)和河北省农林科学院青年基金(A2012060102)项目资助。

Genetic Diversity Analysis of Wild Adzuki Bean Germplasm and Its Relatives by Using SSR Markers

LIU Chang-You1,FAN Bao-Jie1,CAO Zhi-Min1,SU Qiu-Zhu1,WANG Yan1,ZHANG Zhi-Xiao1,CHENG Xu-Zhen2,TIAN Jing1,*   

  1. 1 Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences / Hebei Laboratory of Crop Genetic and Breeding, Shijiazhuang 050031, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-07-01 Revised:2013-09-16 Published:2014-01-12 Published online:2013-10-22
  • Contact: 田静, E-mail: nkytianjing@163.com, Tel: 0311-87670655

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摘要：

利用28对SSR引物对96份野生小豆资源、小豆近缘野生植物及栽培小豆进行遗传多样性分析，共检测到255个等位变异，平均每对SSR引物9.10个，多态信息含量的变异范围从0.374到0.865，平均为0.722。野生小豆材料和近缘植物Vigna minima遗传变异丰富。来自不同地域的野生小豆材料具有大量特异等位变异，基于非加权成组配对算术平均法的聚类分析可将不同地理来源的野生小豆单独分群，且与主坐标分析的结果相一致。4份栽培小豆材料与日本野生小豆遗传距离较近，表明目前国内小豆育种中较多使用了含有日本血缘的小豆材料，以及国内野生小豆资源的搜集和利用工作落后于日本。本研究对国内野生小豆资源的搜集和保存具有指导意义，并可以为这些资源的评价、利用和优异基因的发掘提供参考。

关键词: 野生小豆, SSR, 小豆近缘植物, 遗传多样性

Abstract:

Wild Adzuki bean (V. angularis var. nipponensis) germplasm and its relatives are very important materials for adzuki bean breeding. In this paper, genetic diversity of 96 accessions of wild types, relatives and cultivated adzuki beans were analyzed by using 28 pairs of polymorphic SSR makers. The results showed that a total of 255 alleles were detected in all the accessions. The average number of allele per SSR locus was 9.10. The range of genetic diversity index (polymorphic information content, PIC value) was from 0.374 to 0.865, with an average of 0.722. The results revealed that there were abundant genetic variations between wild adzuki bean and its relatives Vigna minima. Both cluster and principal coordinate analysis (PCO analysis) suggested that there was a high genetic differentiation between wild adzuki beans and its relatives, and they could be distinguished from each other clearly according to their origins, suggesting that the locations of wild adzuki bean accessions affect their genetic variations. When analyzed the relationship between adzuki bean varieties and wild adzuki bean accessions, we found that their genetic distance was more closer to Japanese wild adzuki bean accessions than to domestic accessions, indicating that more Japanese adzuki bean accessions were used in Chinese adzuki bean breeding, and showing that our works for domestic wild adzuki bean resources collection and use is behind Japan. The present study provides some information in collection and conservation of domestic wild adzuki bean accessions, and the result is useful for evaluating and using these resources to find useful genes.

Key words: Wild adzuki bean, SSR, Relatives of adzuki bean, Genetic diversity

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