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作物学报 ›› 2014, Vol. 40 ›› Issue (03): 457-465.doi: 10.3724/SP.J.1006.2014.00457

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用染色体片段代换系定位陆地棉株高QTL

何蕊1,2,石玉真2,张金凤2,梁燕2,张保才2,李俊文2,王涛2,龚举武2,刘爱英2,商海红2,巩万奎2,白志川1,*,袁有禄2,*   

  1. 1西南大学园艺园林学院, 重庆 400716; 2中国农业科学院棉花研究所 / 棉花生物学国家重点实验室, 河南安阳 455000
  • 收稿日期:2013-07-25 修回日期:2013-11-30 出版日期:2014-03-12 网络出版日期:2014-01-16
  • 通讯作者: 白志川, E-mail: baizhichuan@yahoo.com.cn, Tel: 13983634203; 袁有禄, E-mail: yuanyl@cricaas.com.cn, Tel: 0372-2525371
  • 基金资助:

    本研究由国家自然科学基金项目(31101188), 国家重点基础研究发展计划(973计划)项目(2010CB126006), 国家高技术研究发展计划(863计划)项目(2012AA101108)和中央级公益性科研院所基本科研业务专项(SJA1203)资助。

QTL Mapping for Plant Height Using Chromosome Segment Substitution Lines in Upland Cotton

HE Rui,SHI Yu-Zhen,ZHANG Jin-Feng,LIANG Yan,ZHANG Bao-Cai,LI Jun-Wen,WANG Tao,GONG Ju-Wu,LIU Ai-Ying,SHANG Hai-Hong,GONG Wan-Kui,BAI Zhi-Chuan1,*,YUAN You-Lu2,*   

  1. 1 Horticulture and Landscape College, Southwest University, Chongqing 400716, China; 2 State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
  • Received:2013-07-25 Revised:2013-11-30 Published:2014-03-12 Published online:2014-01-16
  • Contact: 白志川, E-mail: baizhichuan@yahoo.com.cn, Tel: 13983634203; 袁有禄, E-mail: yuanyl@cricaas.com.cn, Tel: 0372-2525371

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摘要：

以陆地棉中棉所36为轮回亲本和海岛棉海1为供体亲本, 构建染色体片段代换系。为了能检测到稳定的株高QTL，将三个代换系群体(BC5F3, BC5F3:4和BC5F3:5)在5个环境中种植，2009年和2010年分别在河南安阳种植BC5F3单株、BC5F3:4株行， 2011年分别在河南安阳、辽宁辽阳和新疆石河子种植BC5F3:4株系。结果表明，在不同群体环境中株高的超亲比例为53.43%~88.97%。从早期构建的总图距为5088.28 cM， 含有2280个SSR标记位点，覆盖26条染色体的遗传连锁图谱中筛选标记，对408个单株进行的SSR鉴定，结果检测到16个株高QTL，分布在10条染色体上。单个QTL解释的表型变异为7.35%~13.17%。有7个QTL在2个以上环境被检测到。与标记MUSS563紧密连锁的qPH-15-19在一个环境中被检测到，在前人的研究中也有报道。这些结果为进一步精细定位QTL、基因克隆、分子辅助选择等研究奠定基础。

关键词: 棉花, 染色体片段代换系, 株高, QTL

Abstract:

Chromosome segment substitution lines (BC5F3, BC5F3:4, and BC5F3:5) were developed with G. hirsutum CCRI36 as the recipient parent and G. barbadense Hai1 as the donor parent. In this study, plant height of BC5F3 individuals (2009 in Anyang Henan), and BC5F3:4 lines (2010 in Anyang Henan) and BC5F3:5 lines (2011 in Anyang Henan, Shihezi Xinjiang and Liaoyang Liaoning, respectively) were evaluated. SSR markers were wed to screen 408 BC5F3 individuals. QTLs for plant height were identified by QTL IciMapping V3.2 software. The  results indicated that the transgressive rate of plant height was from 53.43% to 88.97% and totally 16 QTLs for plant height were detected in three generations and five environments, which were mapped on 10 chromosomes, and explained the phenotypic variance from 7.35% to 13.17%. Seven QTLs were detected in at least two environments. qPH-15-19 was also reported in other researches. These stablly expressed QTLs could be applied in the marker assisted selection, fine QTL mapping and gene cloning.

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