欢迎访问作物学报,今天是

作物学报 ›› 2014, Vol. 40 ›› Issue (03): 476-486.doi: 10.3724/SP.J.1006.2014.00476

• 耕作栽培·生理生化 • 上一篇    下一篇

陆地棉苗期耐盐性的高效鉴定方法

彭振,何守朴,孙君灵,许菲菲,贾银华,潘兆娥,王立如,杜雄明*   

  1. 中国农业科学院棉花研究所 / 棉花生物学国家重点实验室,河南安阳455000
  • 收稿日期:2013-08-06 修回日期:2013-12-05 出版日期:2014-03-12 网络出版日期:2014-01-16
  • 通讯作者: 杜雄明, E-mail: dxm630723@163.com, Tel: 0372-2562252
  • 基金资助:

    本研究由国家科技基础条件平台(2012-012)项目和农业部农作物种质资源保护项目(NB2013-2130135)资助。

An Efficient Approach to Identify Salt Tolerance of Upland Cotton at Seedling Stage

PENG Zhen,HE Shou-Pu,SUN Jun-Ling,XU Fei-Fei,JIA Yin-Hua,PAN Zhao-E,WANG Li-RuDU Xiong-Ming*   

  1. Institute of Cotton Research, Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China
  • Received:2013-08-06 Revised:2013-12-05 Published:2014-03-12 Published online:2014-01-16
  • Contact: 杜雄明, E-mail: dxm630723@163.com, Tel: 0372-2562252

摘要:

利用2个耐盐和2个盐敏感的陆地棉品种,分别设置对照和4% (40 g L–1)浓度NaCl溶液处理三叶期幼苗,处理72 h后调查盐害指数,测定地上部分鲜重、根鲜重、叶片相对含水量、叶绿素荧光参数、相对电导率、丙二醛含量、抗氧化酶类活性等13个与耐盐性相关的重要指标。利用灰色关联聚类、主成分分析和逐步回归等方法综合评价陆地棉苗期耐盐性,认为最大光化学效率(Fv/Fm)可以作为鉴定陆地棉苗期耐盐性的关键指标,构建耐盐指数(y)方程y = 1.943x – 0.882(x = 最大光化学效率),同时结合另外2个耐盐和2个盐敏感品种所得方程y值对耐盐等级进行划分。进一步利用23个已知耐盐性的品种检验方程,计算结果与田间鉴定结果完全一致。因此选用最大光化学效率作为唯一指标鉴定陆地棉苗期耐盐性,高效准确,同时通过构建方程和划分耐盐等级,为未来大规模陆地棉品种资源耐盐性鉴定提供技术标准和研究基础。

关键词: 棉花, 苗期, 耐盐性鉴定, 最大光化学效率, 耐盐指数

Abstract:

Three-leaf cotton seedlings of two salt-tolerant varieties and two salt-sensitive varieties were treated by water and 4% (40 g L–1) NaCl solution, respectively. A total of 13 parameters related to salt tolerance including salt injury index (SII), shoot fresh weight (SRW), root fresh weight (RFW), leaf relative water content (RWC), chlorophyll fluorescence parameters (Fo, Fm, Fv/Fm), relative conductivity (RC), manlondialdehyde (MDA) content and the activity of antioxidant enzymes were monitored after 72h of treatment. Comprehensive assessment of salinity tolerance based on grey relation clustering method, principal component analysis and stepwise regression analysis indicated that themaximum quantum yield of PSII was the most significant correlated indices with salt tolerance in upland cotton, which could be used as a single parameter to assess salt tolerance based on the equation: y = 1.943x – 0.882 (where y is the salt tolerance index, x is the related value of maximum efficiency of photosystem II). The other two salt-tolerant varieties and two salt-sensitive varieties were used to rate the classification of salt tolerance. Salt tolerance index (y) of 23 varieties with known salt tolerance were calculated to validate the accuracy of the equation, the result was consistent with field investigation. In this study, the maximum efficiency of photosystem II was used as the main index to validate the salt tolerance in cotton, together with the construction of salt tolerance index equation and salt tolerance rating, which could greatly improve the efficiency of salinity tolerance evaluation for massive germplasm in future.

Key words: Upland cotton, Seedling stage, Salt tolerance evaluation, Maximum quantum yield of PSII, Index of salinity tolerance

[1]黄滋康, 季道藩, 潘家驹. 中国棉花遗传育种学. 济南: 山东科学技术出版社, 2003. pp 533–544



Huang Z K, Ji D F, Pan J J. Genetics and Breeding of Cotton in China. Jinan: Shandong Science and Technology Press, 2003. pp 533–544 (in Chinese)



[2]张国伟, 路海玲, 张雷, 陈兵林, 周治国.棉花萌发期和苗期耐盐性评价及耐盐指标筛选. 应用生态学报, 2011, 22: 2045–2053



Zhang G W, Lu H L, Zhang L, Chen B L, Zhou Z G. Salt tolerance evaluation of cotton (Gossypium hirsutum L.) at its germinating and seedling stages and selection of related indices. Chin J Appl Ecol, 2011, 22: 2045–2053 (in Chinese with English abstract)



[3]辛承松, 董合忠, 唐薇, 温四民. 棉花盐害与耐盐性的生理和分子机制研究进展. 棉花学报, 2005, 17: 309–313



Xin C S, Dong H Z, Tang W, Wen S M. Physiological and molecular mechanisms of salt injury and salt tolerance in cotton. Cotton Sci, 2005, 17: 309–313 (in Chinese with English abstract)



[4]Basal H. Response of cotton (Gossypium hirsutum L.) genotypes to salt stress. Pak J Bot, 2010, 42: 505–511



[5]Basel S. Salt stress alters physiological indicators in cotton (Gossypium hirsutum L.). Soil Environ, 2012, 31:113–118



[6]Higbie S M, Wang F, Stewart M D, Sterling T M, LindemannW C, Hughs E, Zhang J F. Physiological response to salt (NaCl) stress in selected cultivated tetraploid cottons. Intl J Agron, 2010, 1: 1–10



[7]Shaheen H L, Shahbaz M. Salt-induced effects on some key morpho-physiological attributes of cotton (Gossypium hirsutum L.) at various growth stages. Soil Environ, 2012, 31: 125–133



[8]孙小芳, 刘友良. 棉花品种耐盐性鉴定指标可靠性的检验. 作物学报, 2001, 27: 794–796



SunXF, Liu YL.Test on criteria of evaluating salt tolerance of cotton cultivars. Acta Agron Sin, 2001, 27: 794–796 (in Chinese with English abstract)



[9]李寒暝, 白灯莎•买买提艾力, 张少民, 阿依夏木•沙吾尔, 蒋平安. 新疆棉花品种的耐盐性综合评价. 核农学报, 2010, 24: 160–165



Li H M, Baidengsha M M T A L, Zhang S M, A Yixiamu S W E, Jiang P A. Evaluation of salt resistance of seven cotton (Gossypium hirsutum L.) varieties in Xinjiang. J Nucl Agric Sci, 2010, 24: 160–165 (in Chinese with English abstract)



[10]刘国强, 香黎明, 刘金定. 棉花品种资源耐盐性鉴定研究. 作物品种资源, 1993, 2: 21



Liu G Q, Lu L L, Liu J D. Salt tolerance of cotton germplasm identification. Crop Genet Resour, 1993, 2: 21 (in Chinese)



[11]张保青, 杨丽涛, 李杨瑞. 自然条件下甘蔗品种抗寒生理生化特性的比较. 作物学报, 2011, 37: 496–505



Hang B Q, Yang L T, Li Y R. Comparison of physiological and biochemical characteristics related to cold resistance in sugarcane under field conditions. Acta Agron Sin, 2011, 37: 496–505 (in Chinese with English abstract)



[12]刘少卿, 何守朴, 米拉吉古丽, 周忠丽, 孙君灵, 杜雄明. 不同棉花种质资源耐热性鉴定. 植物遗传资源学报, 2013, 14: 219–220



Liu S Q, He S P, Milajiguli, Zhou Z L, Sun J L, Du X M. Identification for the thermotolerance of different germplasm in cotton. J Plant Genet Resour, 2013, 14: 214–221 (in Chinese with English abstract)



[13]胡标林, 扬平, 万勇, 李霞, 罗世友, 罗向东, 谢建坤. 东乡野生稻BILs群体苗期抗寒性综合评价及其遗传分析. 植物遗传资源学报, 2013, 14: 249–255



Hu BL, Yang P, Wan Y, Li X, Luo S Y, LuoX D, Xie J K. Comprehensive assessment of drought resistance of BILs population derived from Dongxiang wild rice (Oryza rufupogon Griff.) at seedling stage and its genetic analysis. J Plant Genet Resour, 2013, 14: 249–256 (in Chinese with English abstract)



[14]胡标林, 余守武, 万勇, 张铮, 邱兵余, 谢建坤. 东乡普通野生稻全生育期抗旱性鉴定. 作物学报, 2007, 33:425–432



Hu B L, Yu S W, Wan Y, Zhang Z, Qiu B Y, Xie J K. Drought-resistance identification of dongxiang common wild rice (Oryza rufipogon Griff.) in whole growth period. Acta Agron Sin, 2007, 33: 425–432 (in Chinese with English abstract)



[15]孙晓东, 胡劲松, 焦玥. 基于主成分分析和灰色关联聚类分析的指标综合方法研究. 中国管理科学, 2005, 13: 18–22



SunX D, Hu J S, Jiao Y. Research on index integration method based on principal component analysis and grey relation clustering analysis. Chin J Manag Sci, 2005, 13: 18–22 (in Chinese with English abstract)



[16]杜雄明, 孙君灵, 周忠丽, 贾银华, 潘兆娥, 何守朴, 庞保印, 王立如. 棉花种质资源收集、保存评价与利用现状及未来. 植物遗传资源学报, 2012, 13: 163–168



Du X M, Sun J L, ZhouZ L, Jia Y H, Pan Z E, He S P, Pang B Y, Wang L R. Current situation and the future in collection, preservation, evaluation and utilization of cotton germplasm in China. J Plant Genet Resour, 2012, 13: 163–168 (in Chinese with English abstract)



[17]河北省地方标准. 棉花耐盐性鉴定评价技术规范(DB13/T 1339-2010). 唐山:河北省质量技术监督局, 2011



The local standard for Hebei Province. Rules for characterization and evaluation of cotton salt tolerance (DB13/T1339-2010). Tangshan: Hebei Provincial Administration of Quality and Technical Supervision, 2011 (in Chinese).



[18]陈永坤, 汪宇. PEG模拟干旱胁迫对漾濞核桃幼苗抗性物质的影响. 西南林业大学学报, 2013, 33:103–106



Chen Y K, Wang Y. Effects of drought stress simulated by PEG on resistant substances of Juglans sigillata seedlings. J Southwest For Univ, 2013, 33:103–106 (in Chinese with English abstract)



[19]Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol, 1995, 61: 32–42 (in Chinese)



[20]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000



Li H S. Plant physiological and Biochemical Experiment Principle and Technology. Beijing: Higher Education Press, 2000 (in Chinese)



[21]李忠光, 李江鸿, 杜朝昆, 黄号栋, 龚明. 在单一提取系统中同时测定五种植物抗氧化酶. 云南师范大学学报, 2002, 22: 44–45



Li Z G, Li J H, Du C K, Huang H D, Gong M. Simultaneous measurement of five antioxidant enzyme activities using a single extraction system. J Yunan Norm Univ (Nat Sci Edn), 2002, 22: 44–45 (in Chinese with English abstract)



[22]钮福祥, 华希新, 郭小丁, 邬景禹, 李洪民, 丁成伟.甘薯品种抗旱性生理指标及其综合评价初探. 作物学报, 1996, 22: 293–295



Niu F X, Hua X X, Guo X D, Wu J Y, Li H M, Ding C W. Studies on several physiological indexes of the drought resistance of sweet potato and its comprehensive evaluation. Acta Agron Sin, 1996, 22: 293–295 (in Chinese with English abstract)



[23]孙效功, 杨作升. 基于灰色关联度的聚类分析方法. 青岛海洋大学学报, 1995, 25: 229–231



Sun X G, Yang Z S. Cluster analysis based on the gray relational grades. Period Ocean Univ China, 1995, 25: 229–231 (in Chinese with English abstract)



[24]吴凤祥. 多指标评价中指标权重的计算方法研究. 河北林业科技, 1992, 3: 44–46



Wu F X. Multi-index evaluation index weight calculation method. J Hebei For Sci Technol, 1992, 3: 44–46 (in Chinese)



[25]Ashraf M, Ahmad S. Influence of sodium chloride onion accumulation, yield components and fibre characteristics in salt-tolerant and salt-sensitive lines of cotton (Gossypium hirsutum L.). Field Crops Res, 2000, 66: 115–127



[26]张锦伟, 许键, 杨改刚, 谭学林. 用不同浓度NaCl溶液筛选水稻苗期耐盐抗旱材料. 西南农业学报, 2004, 17: 81–83



Zhang J W, Xu J, Yang G G, Tan X L. Screening of rice lines in salt and drought tolerance with NaCl solutions. Southwest China J Agricl Sci, 2004, 17:81–83(in Chinese with English abstract)



[27]解松峰, Kansaye A, 杜向红, 聂小军, 方桂英. 30份引进大麦品种(系)苗期耐盐性综合分析.草业科学, 2010, 27: 127–133



Xie S F, Aly K, Du X H, Nie X J, Fang G Y. Comprehensive analysis of salt tolerance of 30 introduced barley varieties or lines in seedling period. Pratac Sci, 2010, 27: 127–133 (in Chinese with English abstract)



[28]张国新, 王秀萍, 鲁雪林, 刘雅辉. 隶属函数法鉴定水稻品种耐盐性. 安徽农学通报, 2011, 17: 36–39



Zhang G X, Wang X P, Lu X L, Liu Y H. Identification of membership functions salt tolerance of rice varieties. Anhui Agric Sci Bull, 2011, 17: 36–39 (in Chinese)



[29]刘雅辉, 王秀萍, 张国新, 鲁雪林, 张亚丽.棉花苗期耐盐生理指标的筛选及综合评价. 中国农学通报, 2012, 28: 73–78



Liu Y H, Wang X P, Zhang G X, Lu X L, Zhang Y L. Study on selection of physiological indices for salt tolerance and comprehensive evaluation of cotton during seedling stage. Chin Agric Sci Bull, 2012, 28: 73–78 (in Chinese with English abstract)



[30]Ashraf M. Breeding for salinity tolerance in plants. Critical Rev Plant Sci, 1994, 13:17–42



[31]王俊娟, 王德龙, 樊伟莉, 宋贵方, 王帅, 叶武威. 陆地棉萌发至三叶期不同生育阶段耐盐特性. 生态学报, 2011, 31: 3720–3726



Wang J J, Wang D L, Fan W L, Song G F, Wang S, Ye W W. The characters of salt-tolerance at different growth stages in cotton. Acta Ecol Sin, 2011, 31: 3720–3727 (in Chinese with English abstract)



[32]Powle S B. Photo inhibition of photosynthesis induced by visible light. Rev Plant Physiol, 1984, 35: 15–44



[33]林世青, 许春辉, 张其德, 徐黎, 毛大璋, 匡廷云. 叶绿素荧光动力学在植物抗性生理学、生态学和农业现代化中的应用. 植物学通报, 1992, 9: 1–16



Lin S Q, Xu C H, Zhang Q D, Xu L, Mao D Z, Kuang T Y. Some application of chlorophyll fluorescence kinetics to plant stress physiologyphy toecology and agricultural modernization. Chin Bull Bot, 1992, 9: 1–16 (in Chinese with English abstract)



[34]Kalaji H M, Govindjee, Bosa K, Ko?cielniak J, ?uk-Go?aszewska K. Effects of salt stress on photosystem II efficiency and CO2 assimilation of two Syrian barley landraces. Environ Exp Bot, 2011, 73: 64–72



[35]王仁雷, 华春, 刘友良. 盐胁迫对水稻光合特性的影响. 南京农业大学学报, 2002, 25: 11–14



Wang R L, Hua C, Liu Y L. Effect of salt stress on photosynthetic characteristics in rice. J Nanjing Agric Univ, 2002, 25: 11–14 (in Chinese with English abstract)



[36]Everard J D, Gucci R, Kann S C, Flore J A, Loescher W. H. Gas exchange and carbon partitioning in the leaves of celery (Aptium gravealens L.) at various levels of root zone salinity. Plant Physiol, 1994, 106: 281–292



[37]张国伟, 张雷, 唐明星, 周玲玲, 陈兵林, 周治国. 土壤盐分对棉花功能叶气体交换参数和叶绿素荧光参数日变化的影响. 应用生态学报, 2011, 22: 1772–1773



Zhang G W, Zhang L, Tang M X, Zhou L L, Chen B L, Zhou Z G. Diurnal variation of gas exchange and chlorophyll fluorescence parameters of cotton functional leaves under effects of soil salinity. Chin J Appl Ecol, 2011, 22: 1772–1773 (in Chinese with English abstract)



[38]Li G, Wan S W, Zhou J, Yang Z Y, Qin P. Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content, malondialdehyde and proline accumulation responses of castor bean (Ricinusco mmunis L.) seedlings to salt stress levels. Indust Crops Products, 2010, 31: 13–19

[1] 周静远, 孔祥强, 张艳军, 李雪源, 张冬梅, 董合忠. 基于种子萌发出苗过程中弯钩建成和下胚轴生长的棉花出苗壮苗机制与技术[J]. 作物学报, 2022, 48(5): 1051-1058.
[2] 孙思敏, 韩贝, 陈林, 孙伟男, 张献龙, 杨细燕. 棉花苗期根系分型及根系性状的关联分析[J]. 作物学报, 2022, 48(5): 1081-1090.
[3] 闫晓宇, 郭文君, 秦都林, 王双磊, 聂军军, 赵娜, 祁杰, 宋宪亮, 毛丽丽, 孙学振. 滨海盐碱地棉花秸秆还田和深松对棉花干物质积累、养分吸收及产量的影响[J]. 作物学报, 2022, 48(5): 1235-1247.
[4] 郑曙峰, 刘小玲, 王维, 徐道青, 阚画春, 陈敏, 李淑英. 论两熟制棉花绿色化轻简化机械化栽培[J]. 作物学报, 2022, 48(3): 541-552.
[5] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[6] 张艳波, 王袁, 冯甘雨, 段慧蓉, 刘海英. 棉籽油分和3种主要脂肪酸含量QTL分析[J]. 作物学报, 2022, 48(2): 380-395.
[7] 张特, 王蜜蜂, 赵强. 滴施缩节胺与氮肥对棉花生长发育及产量的影响[J]. 作物学报, 2022, 48(2): 396-409.
[8] 赵文青, 徐文正, 杨锍琰, 刘玉, 周治国, 王友华. 棉花叶片响应高温的差异与夜间淀粉降解密切相关[J]. 作物学报, 2021, 47(9): 1680-1689.
[9] 岳丹丹, 韩贝, Abid Ullah, 张献龙, 杨细燕. 干旱条件下棉花根际真菌多样性分析[J]. 作物学报, 2021, 47(9): 1806-1815.
[10] 曾紫君, 曾钰, 闫磊, 程锦, 姜存仓. 低硼及高硼胁迫对棉花幼苗生长与脯氨酸代谢的影响[J]. 作物学报, 2021, 47(8): 1616-1623.
[11] 马欢欢, 方启迪, 丁元昊, 池华斌, 张献龙, 闵玲. 棉花GhMADS7基因正调控棉花花瓣发育[J]. 作物学报, 2021, 47(5): 814-826.
[12] 许乃银, 赵素琴, 张芳, 付小琼, 杨晓妮, 乔银桃, 孙世贤. 基于GYT双标图对西北内陆棉区国审棉花品种的分类评价[J]. 作物学报, 2021, 47(4): 660-671.
[13] 赵佳佳, 乔玲, 武棒棒, 葛川, 乔麟轶, 张树伟, 闫素仙, 郑兴卫, 郑军. 山西省小麦苗期根系性状及抗旱特性分析[J]. 作物学报, 2021, 47(4): 714-727.
[14] 周冠彤, 雷建峰, 代培红, 刘超, 李月, 刘晓东. 棉花CRISPR/Cas9基因编辑有效sgRNA高效筛选体系的研究[J]. 作物学报, 2021, 47(3): 427-437.
[15] 卢合全, 唐薇, 罗振, 孔祥强, 李振怀, 徐士振, 辛承松. 商品有机肥替代部分化肥对连作棉田土壤养分、棉花生长发育及产量的影响[J]. 作物学报, 2021, 47(12): 2511-2521.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!